66 results about "Forensic Pharmacy" patented technology

The invention provides a method for rapidly identifying monoacetylmorphine and morphine in hair. The method sequentially comprises the following steps: 1, placing hair to be detected, an extract liquid, internal standard morphine-d3 and a billiard ball in a crusher, and carrying out micro-crushing treatment; 2, centrifuging a mixture obtained after the micro-crushing treatment; and 3, taking the supernatant of the centrifuged mixture, and carrying out liquid chromatography-tandem mass spectrometry analysis of the supernatant. The method for rapidly identifying monoacetylmorphine and morphine in hair can realize the simple and rapid separation and purification of monoacetylmorphine and morphine molecules in a complex biological matrix, has the advantages of strong specificity, strong selectivity and high sensitivity, can be used for detecting a practical drug addict hair sample, and can also be used for identifying and detecting heroin addict related cases in the forensic toxicological analysis field.

A command center includes at least one network communications interface configured for two-way communications with a plurality of sites remote from the command center and at least one display screen and user interface. Each of the plurality of sites includes at least one forensic field test device configured to identify individuals using DNA samples from the individuals. The display screen and user interface are configured to depict aspects of forensic field test devices of the plurality of sites, wherein the aspects include a site identifier for each of the forensic field test devices and one or more additional aspects.

The invention belongs to the technical field of forensic genetics, and particularly relates to a human Y chromosome STR and SNP genetic marker joint detection system and detection method based on NGS.In order to construct a Y chromosome database, the specific Y chromosome STR and Y chromosome SNP markers of men are selected to successfully construct a joint detection system of 50 Y-STR genetic markers and 150 Y-SNP genetic markers, so that various genetic information can be detected at one time, and technical support is provided for domestic forensic NGS technology detection and rapid development of related fields.

The invention relates to a method for rapidly quantifying synthetic cathinone drugs in urine. The method comprises the following steps: by taking methcathinone-D3 and SKF525A as internal standard substances, extracting synthetic cathinone drugs in urine by using hydrophobic magnetic beads through a magnetic dispersive solid-phase extraction (MDSPE), rapidly quantifying by combining a real-time direct analysis high-resolution mass spectrometry (DART-HRMS), and obtaining a quantifying result within one minute after sample introduction, wherein a to-be-detected substance is good in stability under various conditions, the correlation coefficient is larger than 0.99, the deviation of precision and accuracy is smaller than 15%, rapid quantification of the synthesized cathinone drugs in urine is achieved, detection is rapid and convenient, and a new and effective technical support can be provided for rapidly and quantitatively synthesizing cathinone drugs in forensic poison analysis.

Manually operated pipettors, widely used in clinical, forensics, pharmaceutical research, hospital and biotech laboratories to transfer small volumes of liquid, may be subject to positional errors, operator use errors and hidden performance degradation. Manual pipette performance cannot be accepted without monitoring and reporting. This invention concerns a computer controlled vision tracking and lighting system working in conjunction with a sensor controlled fluid dispensing device and controller to confirm pipette tip positional locations during aspiration and dispensing operations with automatic monitoring of liquids entering and leaving a pipette apparatus to digitally track a manual pipetting operation with a digital output file of validated liquid transfer results. The invention may also monitor possible error conditions and prevent improper liquid transfers during the manual process.

The invention provides a method for rapidly identifying monoacetylmorphine and morphine in hair. The method sequentially comprises the following steps: 1, placing hair to be detected, an extract liquid, internal standard morphine-d3 and a billiard ball in a crusher, and carrying out micro-crushing treatment; 2, centrifuging a mixture obtained after the micro-crushing treatment; and 3, taking the supernatant of the centrifuged mixture, and carrying out liquid chromatography-tandem mass spectrometry analysis of the supernatant. The method for rapidly identifying monoacetylmorphine and morphine in hair can realize the simple and rapid separation and purification of monoacetylmorphine and morphine molecules in a complex biological matrix, has the advantages of strong specificity, strong selectivity and high sensitivity, can be used for detecting a practical drug addict hair sample, and can also be used for identifying and detecting heroin addict related cases in the forensic toxicological analysis field.

The invention belongs to the field of forensic DNA examination and analysis and in particular relates to a miniSTR (short tandem repeat) typing kit used for trace degradation of biological samples. The kit adopts ZNA primers instead of traditional DNA primers and simultaneously carries out multiplex amplification on 12 miniSTR loci and a gender recognition locus, wherein the 12 miniSTR loci are D20S1082, D6S474, D12ATA63, D9S1122, D2S1776, D1S1627, D3S4529, D2S441, D4S2364, D3S3053, D17S1301 and D21S11; and the gender recognition locus is Amelogenin. The STR typing kit provided by the invention combines the miniSTR typing technology with ZNA primer amplification, is suitable for the Chinese population and the minority population, is suitable for trace and high degradation of biological samples and has the effect of greatly improving the typing sensitivity and accuracy.

The present invention provides a rapid, sensitive method for forensic drug testing in a post-mortem subject using oral fluid collected from the post-mortem subject. The method comprises collecting a sample of oral fluid from a post-mortem subject, analyzing the oral fluid sample qualitatively to detect the presence of one or more non-naturally occurring drugs, analyzing the oral fluid sample quantitatively to determine concentration of the one or more non-naturally occurring drugs in the post-mortem subject, and identifying the one or more non-naturally occurring drugs in the post-mortem subject. The detection and quantification in oral fluid is more sensitive and faster than detection and quantification of the non-naturally occurring drugs in blood, urine, bile, and liver tissue collected from the same post-mortem subject. Further, the qualitative and quantitative results are obtained in as little as three hours.

This invention relates to an evidence collection and storage apparatus for use in forensic examination and sample collection processes. The apparatus consists of a container and a swab, the swab including a handle portion, shaft portion and a removable head portion, wherein the container includes a cavity adapted to store an object or substance, a lid including a portion of nanomesh and a swab head portion removing means that engages the head portion of the swab within the cavity, enabling the head portion of the swab to be removed or partially removed from the shaft portion of the swab and stored in the container.

The invention relates to the technical field of medical refrigerating boxes, in particular to a vacuum suction storage forensic extract refrigerating box. The forensic extract refrigerating box comprises a refrigerating box shell and a storage box mounted in the refrigerating box shell, the storage box internally comprises a plurality of partition boxes, a storage cavity is formed in the surface of the side wall of each partition box, and a communicating air hole is formed in the center of the inner wall of each storage cavity; and a connecting pipe is arranged at the position, located at the edge of the communicating air hole, of the inner wall of the storage cavity, an air conveying pipe is installed on the communicating air hole in a threaded manner, a vacuum pipeline is installed at one end of the air conveying pipe in a threaded manner, an electromagnetic valve used for controlling connection and disconnection of the pipeline is installed on a branch pipe, and a vacuum pump is installed at one end of a main pipe through an air inlet pipe. The storage box is formed by combining the multiple partition boxes, the storage box is used for independently placing extracts, the partition boxes are conveniently and independently vacuumized while mutual pollution of the extracts is effectively avoided, and the problem that the vacuum environment in the storage box is damaged when the extracts are frequently taken and placed in the storage box is solved.

The invention is suitable for the technical field of forensic material evidence science, and provides a genetic relationship identification method and a terminal device. The method comprises the stepsof obtaining a first allele and a second allele of a to-be-identified person, and determining a first gene frequency of the first allele and a second gene frequency of the second allele; obtaining afirst occurrence frequency of the first allele in a controlled ancestor and a second occurrence frequency of the second allele in the controlled ancestor; obtaining a genetic relationship level of theto-be-identified person and the controlled ancestor; and according to the genetic relationship level, the first gene frequency, the second gene frequency, the first occurrence frequency and the second occurrence frequency, determining a first genetic relationship between the to-be-identified person and the controlled ancestor. Therefore, the identification process can be simplified.

The invention discloses a method for constructing an old bloodstain miRNA high-throughput sequencing library, including the steps of pretreatment of old bloodstain samples, miRNA extraction and miRNA sequencing library construction, wherein the library construction includes: ① connection of miRNA 5' and 3' adapters; ②Reverse transcription; ③PCR amplification; ④Agarose gel electrophoresis of PCR amplification products, gel cutting and recovery of 145-160bp fragments to obtain the sequencing library. The method of the present invention can be used to complete the construction of the old bloodstain miRNA library within 48 hours, at least only 5cm 2 Old blood stains (about 10ng‑100ng RNA) samples are sufficient. It is conducive to the reuse of old forensic evidence, fills the gap in the field of forensic science, and provides strong technical support for expanding the application of miRNA in forensic science.

A workflow for direct qPCR quantification of unprocessed forensic case samples is disclosed herein. 13 pg of DNA have been detected by direct amplification from paper substrates. Direct qPCR quantification of unprocessed forensic case samples and direct STR amplification of unprocessed forensic case samples collected on the same PE swabs will greatly increase the efficiency and capacity of forensic laboratories.