71 results about "Forensic Pharmacy" patented technology

The present invention provides a single self-contained device for collecting, extracting, on-site testing, and transferring for forensic confirmatory analysis, a wide variety of substances including, but not limited to, drugs of abuse, explosives, weapons of mass destruction, food toxins and industrial wastes. Samples can be obtained from a surface by swabbing a suspect area or the testing of solid materials (pills, capsules, powders), air samples and biological and non-biological fluids by placing the substance in the device. The device includes a swab, a retention well including a wash, and analysis technologies that can be, for example, a lateral flow testing system. The swab is rinsed with a wash prior to testing thereby not compromising the chemistry of the detection technologies and allowing for a wide variety of applications under a number of field conditions. Also, the device is a single self-contained unit instead of having a separate reagent droppers or sprays, making it compact and easy to use. Moreover, the device is designed to not only collect and test samples but to seal the originally target analyte, not affected by testing procedures, in a specially designed cap for shipping under chain of custody documentation to a forensic laboratory for confirmatory testing.

The invention provides a method for determining twenty elements in serum and a matched kit. The kit is mainly used for pretreatment and quality control of required samples when the content of twenty elements in serum is determined through an ICP-MS (inductively coupled plasma mass spectrometer). The kit comprises a diluent, a standard solution containing twenty microelements, a quality control sample, an internal standard stock solution and the like. The kit is easy to use during pretreatment of the required samples, low in cost, high in accuracy and precision, good in stability and suitable for detection of clinical high-flux samples. The ICPMS detection kit can be used for detecting the content of toxic elements in human serum, trace toxin pollution to environment and trace stimulant ingredients as well as forensic toxicological detection for human bodies or corpses.

The invention provides a method for rapidly identifying monoacetylmorphine and morphine in hair. The method sequentially comprises the following steps: 1, placing hair to be detected, an extract liquid, internal standard morphine-d3 and a billiard ball in a crusher, and carrying out micro-crushing treatment; 2, centrifuging a mixture obtained after the micro-crushing treatment; and 3, taking the supernatant of the centrifuged mixture, and carrying out liquid chromatography-tandem mass spectrometry analysis of the supernatant. The method for rapidly identifying monoacetylmorphine and morphine in hair can realize the simple and rapid separation and purification of monoacetylmorphine and morphine molecules in a complex biological matrix, has the advantages of strong specificity, strong selectivity and high sensitivity, can be used for detecting a practical drug addict hair sample, and can also be used for identifying and detecting heroin addict related cases in the forensic toxicological analysis field.

The invention belongs to the field of forensic medicine, and discloses a microhaplotype genetic marker for forensic detection and a kit thereof. The invention provides a genetic marker combination consisting of 21 microhaplotypes located on autosomal chromosomes. The genetic marker combination can be effectively applied to forensic detection. Compared with the traditional detection kit, the microhaplotype genetic marker for forensic detection and the kit thereof provided by the invention have high sensitivity, accurate detection result and high applicability in individual identification, can realize individual identification of human biological samples in a relatively short period of time, and wins valuable time for solving of forensic cases.

A method of determining time of death is disclosed. Cardiac troponin I (cTnI) is the most specific marker for the determination of myocardial infarction (MI). Cardiac troponin I has a distinctive temporal degradation profile after death, which is key to its use as a time of death marker in forensic medicine. The method consists of sampling cardiac tissue, extracting a cardiac protein from a homogenate via methods which may include the magnetic microparticles or magnetic microparticles linked to anti-cTnI antibodies, eluting the proteins, separating proteins by electrophoresis, transferring by western blot the different bands of proteins to paper. A comparison / analysis of the concentration and kinetics of degradation of the protein at a given temperature(s) against known standards after time of death provides an accurate measurement of the time of death. The present invention is more accurate and reliable than the rudimentary time of death techniques currently used by medical examiners.

The present invention provides a method and a matched kit for measuring trace elements in human urine, the kit comprises a dilution solution, a standard solution containing 21 trace elements, a quality control product, an internal standard storage solution, and the like. The kit has the advantages of simple sample pretreatment, low cost, high accuracy and the precision and good stability, and is suitable for the detection of high throughput samples in clinical. The kit can be used for detection of nutrient elements and toxic elements in the human urine, environmental supervision, occupation metal exposure, environmental toxic trace pollution detection, microstimulant detection, and forensic toxicological detection of human bodies or corpses.

Disclosed are devices and methods for collection and analyzing biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature of a test subject, while keeping the sample and signature associated with each other. Such devices and methods are used in forensic, human identification, screening, and access control technologies to rapidly process an individual's identity or determine the identity of an individual.

The present disclosure relates to specimen collection devices comprising stabilizing compositions for the collection, preservation, transport, and analysis of evidence samples for forensic analysis.

The invention provides a method for extracting DNA (deoxyribonucleic acid) by alkaline cracking. The method comprises the following steps of: 1, putting testing material into NaOH (sodium hydroxide) solution of which the concentration is 0.05-0.6M; 2, carrying out incubation on solution, wherein the incubation temperature is 90-99 DEG C and the incubation time is 5-10 minutes; 3, oscillating the incubated solution in an oscillator to oscillate; 4, adding Tris-HCl (tris(hydroxymethyl)aminomethane hydrochloride) solution of which the concentration is 0.01-0.1M and the pH value is 5-8 and carrying out centrifugal oscillation, and 5, freezing and storing extract at the temperature of 20 DEG C. Compared with the prior art, the requirements of existing various conventional forensic biological test materials can be met by changing parameters such as the concentration, the pH value, the incubation time, the incubation temperature and the like of reagent, and automatic DNA extraction work can be carried out at the same time by the same equipment under the same experiment conditions.

The invention relates to an agent formula used for collecting and recovering trace amount of DNA by a one-step method and a corresponding kit. The agent formula is mainly characterized in that the agent which is used for collecting and recovering trace amount of DNA and consists of a pH buffer system, salt ions, a surfactant, chelate resin and prolease is adopted, can furthest recover spot forensic material evidence DNA and has good effect on the recovery of sweat fingerprint DNA residual on different materials. The collected DNA can be directly used for the subsequent analysis without the steps of DNA extraction and purification. Compared with the traditional methods, the method has the characteristics of high DNA recovery ratio, simple and convenient operation, low cost, high accuracy and good repeatability, and the developed kit based on the formula can be directly used for collecting DNA samples of the crime scene in the first time.

A command center includes at least one network communications interface configured for two-way communications with a plurality of sites remote from the command center and at least one display screen and user interface. Each of the plurality of sites includes at least one forensic field test device configured to identify individuals using DNA samples from the individuals. The display screen and user interface are configured to depict aspects of forensic field test devices of the plurality of sites, wherein the aspects include a site identifier for each of the forensic field test devices and one or more additional aspects.

The invention discloses a multiplex system, a method for detecting unbalanced mixed samples through the multiplex system and application of the method. The multiplex system comprises primers for amplifying 18 SNP-STR (single nucleotide polymorphism-short tandem repeat) sites, each site comprises a common reverse primer and two specific forward primers for increasing mutation, and the specific sequences of the primers are as shown in the SEQ ID NO.1-54 in the specification. The SNP-STR (single nucleotide polymorphism-short tandem repeat) multiplex amplification system has the advantages that the operation process is simplified, the system can be widely used on the basis of existing forensic evidence conventional inspection equipment, mixed samples of two persons at a forensic scene are detected, the same STR (short tandem repeat) typing samples are distinguished, non-invasive prenatal diagnosis and non-invasive prenatal paternity test are realized, a new technical means is provided, andclues and evidence are provided for solving a case.

The invention belongs to the technical field of forensic genetics, and particularly relates to a human Y chromosome STR and SNP genetic marker joint detection system and detection method based on NGS.In order to construct a Y chromosome database, the specific Y chromosome STR and Y chromosome SNP markers of men are selected to successfully construct a joint detection system of 50 Y-STR genetic markers and 150 Y-SNP genetic markers, so that various genetic information can be detected at one time, and technical support is provided for domestic forensic NGS technology detection and rapid development of related fields.

The invention discloses a construction method of a dated bloodstain miRNA high-throughput sequencing library. The method comprises the steps of pretreatment of a dated bloodstain sample, extraction ofmiRNA and construction of a miRNA sequencing library, wherein the library construction includes: (1) connection of miRNA 5' and 3' junctions; (2) reverse transcription; (3) PCR (Polymerase Chain Reaction) amplification; (4) agarose gel electrophoresis on a PCR amplification product, gel cutting and recovery of fragments of 145 to 160 bp to obtain the sequencing library. By adopting the method disclosed by the invention, construction of the dated bloodstain miRNA library can be finished within 48 hours, and only a 5cm<2> dated bloodstain (containing about 10 to 100ng RNA) sample is needed at least. Reuse of dated forensic evidence is facilitated, the reuse of dated forensic evidence appears in the field of forensic medicine for the first time, and strong technical support is provided for expanding the application of miRNA in forensic medicine.

The invention belongs to the field of new research of forensic medicine, and particularly relates to a composite amplification typing detection system kit and a detection method for 35 new insertion / deletion (InDel) sites in forensic individual identification research. According to the present invention, the primers of InDel sites are respectively subjected to fluorescent labeling with four fluorescent substances (FAM, HEX, TAMRA and ROX), such that the 35 sites are subjected to composite amplification in the same amplification system, and are detected synchronously; the InDel sites are the length polymorphism molecular genetic markers, can be typed by using the capillary electrophoresis method, and are easily applied and promoted in the basic forensic department; the kit is compatible with various biological samples, and has advantages of high sensitivity, strong specificity and simple and quick operation; and the 35 InDel sites have high cumulative individual recognition rate in unrelated healthy individuals, can meet the needs of forensic individual identification, and further can provide new and effective detection reagents for forensic practice.

The invention relates to a method for rapidly quantifying synthetic cathinone drugs in urine. The method comprises the following steps: by taking methcathinone-D3 and SKF525A as internal standard substances, extracting synthetic cathinone drugs in urine by using hydrophobic magnetic beads through a magnetic dispersive solid-phase extraction (MDSPE), rapidly quantifying by combining a real-time direct analysis high-resolution mass spectrometry (DART-HRMS), and obtaining a quantifying result within one minute after sample introduction, wherein a to-be-detected substance is good in stability under various conditions, the correlation coefficient is larger than 0.99, the deviation of precision and accuracy is smaller than 15%, rapid quantification of the synthesized cathinone drugs in urine is achieved, detection is rapid and convenient, and a new and effective technical support can be provided for rapidly and quantitatively synthesizing cathinone drugs in forensic poison analysis.

Manually operated pipettors, widely used in clinical, forensics, pharmaceutical research, hospital and biotech laboratories to transfer small volumes of liquid, may be subject to positional errors, operator use errors and hidden performance degradation. Manual pipette performance cannot be accepted without monitoring and reporting. This invention concerns a computer controlled vision tracking and lighting system working in conjunction with a sensor controlled fluid dispensing device and controller to confirm pipette tip positional locations during aspiration and dispensing operations with automatic monitoring of liquids entering and leaving a pipette apparatus to digitally track a manual pipetting operation with a digital output file of validated liquid transfer results. The invention may also monitor possible error conditions and prevent improper liquid transfers during the manual process.

The invention belongs to the field of forensic new technology research, and particularly relates to a composite amplification typing detection system kit and detection method for suitable for forensicdegradation sample individual identification and sex confirmation application research of 43 insertion / deletion and 1 amelogenin loci. The four different colors of fluorescent substances (FAM, HEX, TAMRA and ROX) are used for carrying out fluorescence labeling grouping on the primers of 44 loci, so that the 44 loci can be simultaneously subjected to composite amplification in the same amplification system. The InDels loci provided by the invention can be used for typing detection of degradation samples due to the fact that amplified fragments are short. The 44 loci contained in the system kithave high accumulated individual identification rate in irrelevant healthy individuals, and can meet the needs of individual identification of forensic degradation biological samples.

The invention provides a method for rapidly identifying monoacetylmorphine and morphine in hair. The method sequentially comprises the following steps: 1, placing hair to be detected, an extract liquid, internal standard morphine-d3 and a billiard ball in a crusher, and carrying out micro-crushing treatment; 2, centrifuging a mixture obtained after the micro-crushing treatment; and 3, taking the supernatant of the centrifuged mixture, and carrying out liquid chromatography-tandem mass spectrometry analysis of the supernatant. The method for rapidly identifying monoacetylmorphine and morphine in hair can realize the simple and rapid separation and purification of monoacetylmorphine and morphine molecules in a complex biological matrix, has the advantages of strong specificity, strong selectivity and high sensitivity, can be used for detecting a practical drug addict hair sample, and can also be used for identifying and detecting heroin addict related cases in the forensic toxicological analysis field.

The invention belongs to the technical field of forensic examination, and specifically relates to a forensic fluorescence composite detection kit based on 9 slow-mutation Y chromosome STR genetic markers. Aiming at the problem that high mutation rate of a conventional Y chromosome STR is not conducive to evolutionary researches and low resolution of a Y chromosome SNP is not conducive to forensicapplication, the invention provides a forensic fluorescence composite detection kit based on 9 slow mutation Y chromosome STR genetic markers, which comprises a composite amplification primer mixtureof 9 slow mutation Y chromosome STR loci, a composite amplification reaction mixture, an allele genotyping standard substance and a standard DNA 9948. The 9 slow mutation Y chromosome STR loci are DYS593, DYS443, DYS645, DYS388, DYS531, DYS596, DYS426, DYS454 and DYS455. The kit of the invention has high sensitivity, good stability and strong repeatability, and can be effectively applied to researches in the fields of forensic family search, population history, population migration evolution and the like.

The invention provides a primer group and a kit for simultaneously amplifying 37 Y-STR gene loci of human and application thereof, and belongs to the technical field of molecular genetics. In the invention, the 37 Y-STR gene loci comprise 36 Y chromosome STR gene loci and 1 Y-indel gene locus; 37 gene loci can be amplified in one reaction at the same time, the compatibility of current public security DNA database comparison is fully met, the amplification time is shortened, the identification efficiency and the detection material adaptability of the kit are improved, and the multifunctional STR identity identification requirements of current forensic identity identification, forensic DNA database construction and judicial genetic relationship identification can be met.

The present invention is related to a method for obtaining dental pulp and root cement in the forensic dentistry field, wherein the method comprises the steps of: (a) obtaining a tooth; (b) taking a digital radiography to the tooth; (c) external rehydrating of the tooth; (d) perforating the rehydrated tooth; (e) internal rehydrating of dentin pulp complex (f) obtaining rehydrated root cement; (g) obtaining rehydrated dental pulp content with a low speed rotation tool; and (h) storing, preservation, processing and / or analyses of the rehydrated dental pulp content and rehydrated root cement, and the use of this method and kits thereof for forensic identification, estimation of post mortem interval (early and late) and determination of possible causes of death.

The invention discloses a primer combination for detecting 617 SNPs and InDel and application of the primer combination in forensic identification and genetic relationship identification. The invention provides a primer group. The primer group is composed of 1234 primers, 1234 primers are sequentially shown as sequences 1 to 1234 in the sequence table. The invention also discloses application of any one of the primer groups in preparation of a forensic identification kit. The invention also protects the application of any one of the primer groups in forensic identification. The invention alsoprotects the application of any one of the primer groups in genetic relationship identification. The amplification product length of the multiplex amplification primer group is 56-225 bp, the averageamplification product length is 176 bp, the number of sites below the average amplification product length is 422, so that the multiplex amplification primer group is more suitable for forensic degradation samples. The multiple amplification primer group is good in balance, and the detection uniformity is about 83%. The method has application value for forensic identification and genetic relationship identification.

A sexual assault evidence collection kit that may be used by the victim or other non-professionals. The kit may be simplified compared to kits for professional use, and may be usable with no specialized training. Victims may therefore be able to collect evidence themselves after an assault, instead of searching for a facility with personnel trained in forensic evidence collection, and enduring a long, intrusive examination. Embodiments may include a package (for example a clear bag) and the following components inside the package: gloves, swabs, swab containers, water, evidence collection bags, evidence bag sealing tape, evidence collection tape, a permanent marker, and instructions on how to identify, collect, seal, document, and store evidence.

The present invention provides a rapid, sensitive method for forensic drug testing in a post-mortem subject using oral fluid collected from the post-mortem subject. The method comprises collecting a sample of oral fluid from a post-mortem subject, analyzing the oral fluid sample qualitatively to detect the presence of one or more non-naturally occurring drugs, analyzing the oral fluid sample quantitatively to determine concentration of the one or more non-naturally occurring drugs in the post-mortem subject, and identifying the one or more non-naturally occurring drugs in the post-mortem subject. The detection and quantification in oral fluid is more sensitive and faster than detection and quantification of the non-naturally occurring drugs in blood, urine, bile, and liver tissue collected from the same post-mortem subject. Further, the qualitative and quantitative results are obtained in as little as three hours.

A longstanding challenge in forensics is maximizing the number of “mini”—short tandem repeat amplicons within the limited confines of fluorescent channels and run length afforded by capillary electrophoresis. The instant disclosure overcomes this longstanding challenge by fusing mini-STR amplicons in a locus specific manner with a DNA construct of defined length. Staggering the lengths of the DNA constructs allows for staggering the mini-STRs in a locus specific manner. Beyond maximizing mini-STRs, the disclosed methods, compositions and kits can dramatically increase throughput of forensic samples by capillary electrophoresis.

The present invention relates to a forensic system and method, including a methodology, for analyzing medical and pharmacy data to determine the legitimacy of controlled substance prescription and use, and to detect fraud, abuse, and / or diversion of controlled substances. In one aspect, said method provides objective evidentiary data that shows with high certainty whether a suspected medical practitioner has been issuing controlled substance prescriptions “outside the usual course of medical practice” and / or “for other than legitimate medical purposes”. Said method rearranges, organizes, and simplifies the complex data in patients' medical and PDMP charts, and provides a series of three sequential work products in spreadsheet form, a Forensic Chronology chart, a Forensic Summary chart, and a Standard of Care Summary chart, via which medical data is effectively translated into objective criteria from which a legal conclusion can be determined by legal scholars, prosecutors, defense attorneys, and juries.