59 results about "Forensic Pharmacy" patented technology

The invention discloses a 26 Multi-InDel genetic marker system for highly degraded sample typing, a detection primer and application of the 26 Multi-InDel genetic marker system, and belongs to the technical field of forensic medicine identification. According to the invention, through bioinformatics screening and experimental verification, a multi-InDel genetic marker system of which the lengths of 26 amplicons do not exceed 125 bp is constructed. The problem that degradation detection material detection and high system efficiency are difficult to consider at the same time is solved. The random matching probability of the system in Han population in Hunan reaches 2.25 * 10 <-17 >, the cumulative paternity exclusion rate is 0.999925, complete typing of highly degraded DNA can be achieved, triad paternity test can be completed, and a new technical scheme which is efficient and compatible with a capillary electrophoresis platform is provided for forensic practice.

The invention relates to the technical field of forensic genomics, in particular to a primer group and a kit for detecting genetic markers of 365 Y chromosomes and application of the primer group and the kit. According to the invention, an amplification primer group for specific 57 Y-STRs and 308 Y-SNPs of Chinese population is optimally designed, and a joint detection method with high sensitivity and high resolution is constructed. Y-STR haplotype analysis and Y-SNP haplogroup accurate typing can be synchronously realized, and the problems of high family checking false positive rate, insufficient geographic ancestor inference resolution and the like caused by single site type detection in the traditional technology are solved. The method is suitable for diversified biological samples such as blood, seminal stains and saliva, and provides a novel technical scheme of high-throughput, low-cost and multi-dimensional paternal genetic information analysis for forensic practice. Comprising construction of a Y-STR and Y-SNP composite amplification system based on next-generation sequencing and application of the Y-STR and Y-SNP composite amplification system in forensic medicine individual recognition, family investigation and biogeography ancestor inference.

A method and apparatus for collecting DNA samples from uncooperative suspects are provided. A modified bathroom fixture, including a toilet with an empty reservoir tank and a missing handle on the water fill valve, retains excreted material in the toilet bowl, allowing for easy collection of DNA samples. In an alternate embodiment, the toilet's reservoir tank is full, and the water fill valve is in the on position, but the waste flush is diverted to a holding tank. This enables DNA technicians to collect samples from the excreted material for forensic analysis. The invention eliminates the need for costly and time-consuming stakeouts, provides a quick and reliable method for DNA sample collection, reduces the risk of contamination, and allows for immediate sample collection, advancing forensic DNA analysis.

This invention relates to the field of gene detection technology and discloses a high-throughput gene sequencing data multivariate quality control system, including a sample information management module, a sequencing data acquisition module, a multivariate quality control analysis module, a result output and traceability module, an equipment collaborative control module, and a data storage module. Each module communicates bidirectionally via a data bus to achieve real-time data transmission and interaction. This invention addresses the dual needs of clinical medical testing and forensic evidence identification, adapting to multiple application scenarios and solving the problem of single-scenario application in existing systems. It can simultaneously meet the quality control requirements for non-invasive prenatal testing, reproductive genetic testing, tumor gene testing, and forensic kinship identification, eliminating the need for multiple quality control systems, reducing equipment costs and operational complexity, and improving laboratory efficiency.

This invention discloses a method for predicting sudden cardiac death based on forensic autopsy data using machine learning and its forensic applications, belonging to the fields of machine learning, statistics, and forensic identification. This invention provides a method for screening independent predictors of forensic diagnosis of sudden cardiac death using LASSO regression and logistic regression. Using 10-fold cross-validation, the method selects the minimum lambda(λ) to identify 14 risk factors. Logistic regression ultimately identifies 9 independent predictors, including age, heart weight, left ventricular wall thickness, right ventricular wall thickness, interventricular septum thickness, aortic valve circumference, mitral valve circumference, liver weight, and left kidney weight. A nomogram and a web-based calculator are constructed for predicting sudden cardiac death in forensic practice, thereby determining sudden cardiac death using objective indicators. This method has not been reported worldwide.

The invention relates to the technical field of biological information acquisition, and discloses a biological information acquisition system for forensic physical evidence, which comprises a multi-dimensional acquisition module and an intelligent management module. According to the system, management data, environmental parameters and development data of all physical evidences are acquired through the multi-dimensional acquisition module and are classified to form a data set, and the intelligent management module analyzes the characteristics of each physical evidence, predicts the development effect, judges the retention state of fingerprint residues and generates penetration scores, so that invalid acquisition is avoided; the intelligent management module is used for judging whether the environmental parameters meet the reaction conditions or not, evaluating the development effect of the physical evidence and generating a reaction score, so that the multi-dimensional analysis perception capability is high; the intelligent management module judges whether the material evidence meets the detection condition of the silver nitrate appearing method, outputs the material evidence grade, then judges the solution type and outputs the corresponding biological information collection suggestion, and the intelligent collection success rate is high.

The invention discloses an interpretation method and system for mixed DNA diallelic SNP data based on WGA-WGS, and relates to the technical field of forensic genetics, and the specific steps are as follows: obtaining intersection SNP sites based on WGA-WGS typing data of a mixed sample and a target individual sample, and generating an SNP site set; determining the number N of mixed contributors and a single allele typing error rate; calling a preset integrated typing error observation probability model based on the application scene, and calculating a total likelihood ratio based on the number N of mixed contributors and a single allele typing error rate on the basis of the SNP site set; the mixed sample is explained based on the total likelihood ratio, the whole process is operated based on a preset mathematical model and objective parameters, and potential bias caused by manual intervention and subjective judgment is reduced to the maximum extent; the scientific preciseness of evidence explanation is ensured, and the repeatability and verifiability of an analysis result are also ensured.

The invention pertains to the use of a specific biomarker of elastin degradation (desmosine) that measures the extent and progression of chronic obstructive pulmonary disease (COPD). In addition to potentially serving as a screening procedure for COPD, it provides a real-time measure of COPD drug efficacy and may therefore supersede the use of less sensitive tests such as pulmonary function studies and computed tomography. Equally important, the current invention constitutes a marked improvement for measuring desmosine in tissues and body fluids by greatly shortening the time for detection of this molecule in liquid chromatography-tandem mass spectrometry (LC-MS-MS) assays that are the gold standard for such measurements. Unlike previous methods, the invention allows for the use of LC-MS-MS without modification, so the method can be applied to any laboratory that uses this equipment for other measurements. This would include forensic facilities that need to determine if undiagnosed COPD played a role in the loss of life.

The present invention provides a rapid method for forensic drug testing in a post-mortem individual using muscle tissue and fluid associated with the muscle tissue obtained from remains of the post-mortem individual. The method comprises obtaining muscle tissue and associated fluid from a post-mortem individual, collecting the fluid associated with the muscle tissue, analyzing the fluid associated with the muscle tissue to detect and quantify one or more non-naturally occurring drugs in the post-mortem individual, and identifying the one or more non-naturally occurring drugs in the post-mortem individual. The detection and quantification of non-naturally occurring drugs in muscle tissue and associated fluid is surprisingly faster than detection and quantification of the non-naturally occurring drugs in muscle tissue obtained from the same post-mortem individual and prepared as muscle tissue homogenates using the LC-MS / MS method, with results obtained in as soon as three hours.

This invention discloses a cSNP primer composition, kit, and its application for body fluid tracing based on next-generation sequencing technology, relating to the fields of forensic biological evidence and molecular identification technology. The cSNP primer composition includes primer pairs amplifying 34 cSNP genetic markers. The primer composition for body fluid tracing based on next-generation sequencing technology provided by this invention relates to 34 cSNP markers for identifying five types of body fluids. This invention has developed and established a kit capable of determining the body fluid type and genotyping the body fluid donor cSNPs. Using the primer composition or kit provided by this invention, highly consistent genotyping results can be obtained, with high sensitivity and good tissue specificity. It provides an effective detection tool for directly associating common body fluid types and body fluid donors, and is of great significance for further research on body fluid identification and achieving accurate and efficient forensic identification related to body fluids.

The utility model discloses a forensic dissection work platform relates to the technical field of dissection table, it includes elevating seat, dissection table is set up in elevating seat, the top of dissection table is opened with recessed cavity, the inner wall of recessed cavity is opened with a plurality of through -opening, the inside of dissection table is opened with chamber relative to the position below recessed cavity, negative pressure component sets up in the bottom of dissection table for forming negative pressure in the chamber, thereby can reduce the diffusion of corpse smell, reduce the inhale of forensic doctor, cause the discomfort, simultaneously, the liquid produced in the dissection process of corpse also can enter into the chamber through through -opening.

1. The name of the design product: liquid chromatography tandem mass spectrometry system. 2. The use of the design product: general analysis instrument, mainly used in clinical detection, biological medicine, food safety, agricultural product detection, environmental monitoring, forensic toxicology and scientific research fields, used for checking various organic molecules, carrying out high-throughput quantitative analysis of test molecules, and giving the accurate content of test substances in samples. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

Automated Saliva- Based Biomarker Detection and Forensic Assessment Device

Compositions, assays, methods, diagnostic methods, kits, and diagnostic kits are disclosed for the specific and differential detection of SARS-COV-2 and / or other viruses from samples, including veterinary samples, clinical samples, food samples, forensic sample, environmental samples (e.g., obtained from soil, garbage, sewage, air, water, food processing and manufacturing surfaces, or likewise), or biological sample obtained from a human or non-human animal.

The invention relates to the technical field of molecular detection, in particular to forensic body fluid identification based on GC-MS metabolites. By means of the identified metabolite, body fluid type identification under the low sample size in a forensic identification scene can be achieved, the method has the advantages of being high in sensitivity, good in reproducibility, nondestructive in detection and the like, and a brand new thought is provided for forensic physical evidence identification.

The application discloses a mixed DNA biallelic SNP data interpretation method and system based on WGA-WGS, and relates to the technical field of forensic genetics, and the specific steps are as follows: obtaining intersection SNP sites based on WGA-WGS typing data of a mixed sample and a target individual sample, and generating a SNP site set; determining the number N of mixed contributors and a single allele typing error rate; calling a preset observation probability model of integrated typing errors based on an application scenario, calculating a total likelihood ratio based on the number N of mixed contributors and the single allele typing error rate on the basis of the SNP site set; and interpreting the mixed sample based on the total likelihood ratio, wherein the whole process in the application is operated based on a preset mathematical model and objective parameters, so that potential bias caused by artificial intervention and subjective judgment is minimized, the scientific rigor of evidence interpretation is ensured, and the repeatability and verifiability of analysis results are also ensured.

The utility model relates to the technical field of medical instruments, in particular to a multifunctional portable forensic inspection container. Comprising a container body, and the container body is a cylindrical container made of a flexible material and is divided into an upper wall folding part and a bottom end rotating part; the outer peripheral wall of the upper wall folding part is provided with a telescopic crease which can be recessed inwards, the side wall is provided with vertical indication scales, the inner wall is slidably connected with a partition plate assembly, and the inner wall of the upper wall folding part is fixedly connected with two symmetrically-arranged suspension hooks. The inner wall side of the bottom end rotating part is provided with a shrinkage crease capable of rotating inwards, the bottom end rotating part is provided with an opening and closing type water filtering opening in a penetrating mode, and the opening and closing type water filtering opening is connected with a sealing cover in a clamped mode. The utility model aims to solve the technical problems that a traditional lung floating experiment container is not portable and cannot meet various operation requirements of forensic doctors in special occasions.

The invention is suitable for the technical field of forensic dentition science, and provides an automatic forensic dentition science individual identification method based on a 3D computer vision algorithm, and the method comprises the steps: carrying out the filtering, denoising and down-sampling processing of an initial 3D dentition model, so as to remove the noise and reduce the data size; segmenting a dental crown region as a region of interest through an automatic algorithm, and removing gingiva and palatal plica soft tissues; and performing rigid registration on the segmented ROI model by adopting an iterative nearest point algorithm, and calculating a root-mean-square error and a registration fitting degree after registration. According to the method, the steps of filtering, denoising and down-sampling preprocessing are added before model segmentation, the 3D dental model segmentation, registration and comparison method is designed based on an open source 3D vision algorithm, full-process automatic processing from model preprocessing, model segmentation, model registration to model comparison is achieved, the registration and comparison precision can be guaranteed, and meanwhile the registration and comparison efficiency is improved. Registration efficiency is greatly improved, computing resources are saved, and subjective errors caused by manual segmentation of a model are avoided.

The invention discloses an autopsy bed special for a forensic. Comprising a first supporting assembly used for supporting the upper body and a second supporting assembly used for supporting the legs. The first supporting assembly comprises a plurality of first telescopic plates which are adjacent in sequence, the second supporting assembly comprises a plurality of second telescopic plates, all the second telescopic plates are arranged side by side to form two groups, all the second telescopic plates of each group of second telescopic plates are adjacent in sequence, and the two groups of second telescopic plates are arranged side by side beside the same first telescopic plate; the two short edges of all the first telescopic plates and the two short edges of all the second telescopic plates are supported on the ground through the lifting supports, and the heights and the horizontal inclination angles of the first supporting assemblies and the second supporting assemblies are adjusted by changing the heights of the lifting supports. All the lifting supports are the same in structure, and each lifting support comprises a telescopic motor, a ball joint bearing and a longitudinal beam. Adjusting functions of lifting, inclining and the like of the bed body are added, and the working difficulty of a forensic is reduced.

The invention belongs to the technical field of judicial expertise, and discloses a forensic clinical identification scar area measuring device and a forensic clinical identification scar area measuring method, and the specific technical scheme is that the forensic clinical identification scar area measuring device and the forensic clinical identification scar area measuring method construct a hardware system, a binocular vision acquisition module, a data processing and control unit and an auxiliary calibration and positioning assembly; establishing an accurate stereoscopic vision coordinate system; according to the method, left and right images of a scar area are synchronously collected through a binocular camera and stereo matching is carried out, a three-dimensional point cloud model is generated, three-dimensional coordinates of all points on the surface of the scar are calculated, curved surface gridding processing is carried out, the real curved surface form of the scar is restored, and the total area is obtained by accumulating the area of a single sheet. The method has the advantages of being high in operation speed and accuracy, and meanwhile has the characteristics of being non-contact, low in cost and suitable for complex curved surfaces of the human body, so that the requirements for objectivity and traceability of judicial expertise are met.

FORENSIC SAMPLE HANDLING MACHINE

The invention discloses a primer combination for body fluid detection based on an RT-LAMP technology, a composite system and application, and belongs to the technical field of forensic medicine identification. The kit comprises a housekeeping gene, a specific mRNA (messenger ribonucleic acid) site and a primer combination for amplifying a corresponding site, each group of primers in the primer combination comprises two inner primers, two outer primers and two loop primers, and the specific sequences of the primers are as shown in SEQ ID NO.1-60. The LAMP primer is designed aiming at the body fluid specific mRNA site, the expression level of mRNA is detected, and then a body fluid sample is recognized. The method is simple to operate, high in specificity, strong in tolerance, strong in recognition capability and short in time consumption, can obtain a body fluid type result within 30 minutes, and can be applied to the field of forensic body fluid identification and the like.

The invention discloses a genetic marker system containing 31 high-performance autosomal microhaplotypes as well as a detection primer and application thereof, and belongs to the technical field of forensic medicine identification. The genetic marker system comprises 31 loci, namely, mh02ZL-022, mh03ZL-014, mh03ZL-015, mh03ZL-016, mh03ZL-017, mh03ZL-018, mh04ZL-014, mh10ZL-010, mh10ZL-01, mh10ZL-012 and the like, and is characterized in that the molecular weight of the genetic marker system is 8000-9000, and the molecular weight of the genetic marker system is 8000-9000. On the basis of traditional micro-haplotype screening, InDels is incorporated into micro-haplotypes for whole genome screening, and an amplification system of a novel composite genetic marker is constructed. The system has the advantages of short fragment, short amplicon, low mutation rate and the like, is wide in applicability, and has good application potential in individual recognition, genetic relationship analysis and mixed sample detection of forensic detection.

The invention provides a preparation method of hydroxycotinin-D3, and aims to solve the problem that existing hydroxycotinin-D3 lacks a reference substance and a deuterated substance and hinders forensic poison identification, 3-(pyrrolidine-2-yl) pyridine is used as a raw material, a product is prepared through deuterium methylation, oxidation, hydroxylation and refining, a final finished product is colorless needle-shaped crystals, and after the colorless needle-shaped crystals are dried at 45 DEG C for 72 hours, the hydroxycotinin-D3 is obtained. According to the present invention, the yield is 56% based on the crude product, the HPLC detection results show that the purity is more than 99.7%, the single impurity content is less than 0.1%, the total impurity content is less than 0.3%, the whole product has high stability and high reaction specificity from the intermediate to the finished product, the final finished product completely meets the strict requirements of the reference substance for forensic poison identification, the prepared hydroxycotinin-D3 has advantages of high reaction efficiency, good product quality and the like.

The invention discloses a detection method for forensic body fluid traceability. According to the method, peripheral blood, menstrual blood, vaginal secretion, semen and saliva are detected by using an RPA-CRISPR-Cas12a-LFD detection system and utilizing mRNA markers of HBB, MMP10, DKK4, AKAP4 and HTN3 genes. The detection method can realize accurate traceability of the sample and effective detection of the mixed body fluid, is short in detection time, can complete the test only in 40 minutes, is high in sensitivity, simple and convenient to operate and high in body fluid traceability efficiency, gets rid of dependence on large-scale equipment, and is beneficial to popularization and application in primary forensic DNA laboratories and biological research laboratories.

The present application relates to the field of forensic pathology, forensic genetics and molecular identification technology, in particular to a primer composition for evaluating genetic susceptibility of unexplained sudden death, products and applications thereof. The primer composition provided by the present application comprises nucleotide sequences such as amplification primers shown in SEQ ID NO. 1-512, that is, 256 pairs of primers related to 238 quantitative trait loci. The present application further provides products and methods for evaluating genetic susceptibility of unexplained sudden death of individuals. Experimental results show that the primer composition or kit provided by the present application can detect consistent and highly repeatable genotyping results, and on this basis, the genetic susceptibility of unexplained sudden death can be further evaluated by calculating the polygenic risk score, which has important significance for the genetic background research of unexplained sudden death and the accurate cause of death identification in sudden death related cases in forensic identification work.

When the image acquisition module is used, the left-right position of the camera can be adjusted through the transverse movement mechanism, the vertical position of the camera can be adjusted through the vertical movement mechanism, and the front-back position of the camera can be adjusted through the longitudinal movement mechanism; the camera rotating mechanism drives the camera to rotate in the front-back direction, and the bottom light source and the side light source are matched, so that the consumable on the consumable frame, the liquid image in the consumable and the liquid image in the suction head of the automatic pipettor can be collected. The image acquisition device can acquire images of consumables and liquid in the consumables and images of liquid in a suction head of an automatic pipettor, is suitable for a forensic biochemical test full-automatic extraction and detection platform, can complete monitoring and recording of various automatic pipetting processes in cooperation with an image identification module, and improves test accuracy and reliability.

This invention relates to the interdisciplinary field of artificial intelligence and forensic medicine, and discloses a system for analyzing and reasoning about the injury mechanism of craniocerebral injury by integrating a multimodal large-scale model. The system includes a multimodal data preprocessing and alignment module, a multimodal large-scale model inference engine, an intelligent report generation module, and an interactive natural language query interface. This system, by constructing a multimodal data preprocessing and alignment module, for the first time spatially registers biomechanical simulation stress cloud maps as an independent visual modality with cranial CT images in a standard anatomical coordinate system. Combined with forensic autopsy text and case descriptions, a multimodal encoder is used to achieve deep fusion of heterogeneous data from three sources—image, mechanical, and text—in a shared semantic space. This effectively breaks down the barriers of isolated information and semantic fragmentation among different modalities in traditional forensic analysis, providing a structured and aligned input foundation for subsequent high-precision injury mechanism inference.

The application belongs to the technical field of forensic medicine, and discloses an East Asian group fine division AIM-SNP typing system and application. The application is based on a machine learning algorithm, systematically screens and optimizes a set of biogeographical ancestral tracing markers in the whole genome range, optimizes 58 high ancestral information amount SNP sites from 2135 candidate di-allele SNPs, provides a core feature set for accurate tracing, and further screens 2 Y chromosome deletion / insertion sites and 1 tooth enamel gene for gender identification. The feature set can accurately trace to five major continental populations, namely, Africa, Europe, East Asia, South Asia and America (excluding mixed American descent). In particular, the problem of insufficient tracing resolution in the East Asian population is solved, and the ancestral resolution capability of the East Asian population is significantly improved, making it more applicable and feasible in Chinese forensic practice.

A portable repositioning evidence support stand for supporting forensic evidence primarily for 3D scanning applications. The evidence support stand features a base plate containing a number of mounting apertures, a number of support members, and a number of fasteners. A method for adjusting support of a piece of forensic evidence for 3D scanning, featuring: positioning a plurality of support members of an evidence support stand into a predetermined position, adjusting the height of each support member, securing each support member to the base plate with a fastener, and providing the piece of forensic evidence to bumper pads on the support members.