41 results about "Forensic dna" patented technology

Described herein are methods and devices for nucleic acid quantification and, in particular, to microfluidic methods and devices for nucleic acid quantification. In certain embodiments methods of quantifying a target nucleic acid without the need for amplification are provided. The methods involve, in some embodiments, allowing a binding agent to become immobilized with respect to the target nucleic acid. In some cases, the binding agent comprises a signaling moiety that can be used to quantify the amount of target nucleic acid. In another aspect, the quantification can be carried out rapidly. For example, in certain embodiments, the quantification can be completed within 5 minutes. In yet another aspect, samples containing a low amount of target nucleic acid can be quantified. For instance, in some cases, samples containing less than 100 nanograms per microliter may be quantified. Also described are devices and kits for performing such methods, or the like.

The invention belongs to the field of forensic DNA examination and analysis and in particular relates to a miniSTR (short tandem repeat) typing kit used for trace degradation of biological samples. The kit adopts ZNA primers instead of traditional DNA primers and simultaneously carries out multiplex amplification on 12 miniSTR loci and a gender recognition locus, wherein the 12 miniSTR loci are D20S1082, D6S474, D12ATA63, D9S1122, D2S1776, D1S1627, D3S4529, D2S441, D4S2364, D3S3053, D17S1301 and D21S11; and the gender recognition locus is Amelogenin. The STR typing kit provided by the invention combines the miniSTR typing technology with ZNA primer amplification, is suitable for the Chinese population and the minority population, is suitable for trace and high degradation of biological samples and has the effect of greatly improving the typing sensitivity and accuracy.

The invention discloses a short tandem repeat (STR) parting standard substance, which is a deoxyribonucleic acid (DNA) mixture and comprises 31 DNA segments with the nucleotide sequences as 1) to 31). In the invention, all allelic genes in the bits of Chinese people groups are researched and studied through focusing on the discovered STR gene bits in the existing forensic molecular genetics, and in addition, the biostatistics technology is used for studying the gene frequency distribution of each allelic gene. On the basis, the experiments techniques such as molecular genetics, molecule biology, cell biology, genetic engineering and the like are integrally utilized, the allelic gene DNA information base is developed and built for the first time, all of the allelic gene DNA segments such as STR bits are included, further, the forensic DNA standard reference system independently developed by China is further built, and the special artificially synthesized STR standard substance is formed.

The invention belongs to the field of biotechnology, and relates to a compound amplification system, a kit and an application of rapidly mutating Y chromosome short tandem repeat sequences. The compound amplification system includes 16 pairs of primers, which can simultaneously amplify 16 STR sites: DYS630, DYS464, DYF403S1b, DYF399S1, DYS518, DYF403S1a, DYS527, DYS713, DYS612, DYS626, DYS627, DYS526, DYF404S1, DYF387S1, DYS449, DYS547. Compared with the prior art, the present invention has the following advantages: 1. Contains 16 STRs with high mutation rate of human male Y chromosome, which is unique and novel, with large amount of information and good compatibility. 2. It has wide adaptability, strong directivity and high precision, and is suitable for forensic DNA analysis in all cases involving male cellular evidence. All biological samples containing male cells, such as blood stains, semen spots, saliva, hair, nails, cartilage and other human tissues, can be identified. 3. The system has good specificity and stability. After repeated verifications, no non-specific amplification products are produced, and the signal intensity is stable. 4. High sensitivity, the amount of DNA template as low as 15 pg can be accurately typed.