11 results about "Forensic dna" patented technology

A method and apparatus for collecting DNA samples from uncooperative suspects are provided. A modified bathroom fixture, including a toilet with an empty reservoir tank and a missing handle on the water fill valve, retains excreted material in the toilet bowl, allowing for easy collection of DNA samples. In an alternate embodiment, the toilet's reservoir tank is full, and the water fill valve is in the on position, but the waste flush is diverted to a holding tank. This enables DNA technicians to collect samples from the excreted material for forensic analysis. The invention eliminates the need for costly and time-consuming stakeouts, provides a quick and reliable method for DNA sample collection, reduces the risk of contamination, and allows for immediate sample collection, advancing forensic DNA analysis.

The application discloses a human DNA sample quantitative amplification system and application thereof, and the amplification system comprises a primer and probe compound for specifically amplifying four target sequences; wherein the four target sequences are two autosomal target genes CSF1PO and TPOX, one Y chromosome target gene SRY and one non-human synthetic oligonucleotide IPC. Advantages are as follows: (1) the amplification system and the kit thereof can be applied to all forensic DNA analysis, four different target genes can be simultaneously amplified in one tube, human total DNA and human male DNA can be quantitatively and qualitatively evaluated, and information about sample degradation and inhibitor condition is provided. The primer and probe are high in specificity and sensitivity, and the minimum detection limit can reach 1 pg level. (2) the kit effectively simplifies the steps of qPCR reaction operation, improves the accuracy and precision of the test, and is suitable for all collected sample sources in forensic DNA analysis.

The invention discloses a detection method for forensic body fluid traceability. According to the method, peripheral blood, menstrual blood, vaginal secretion, semen and saliva are detected by using an RPA-CRISPR-Cas12a-LFD detection system and utilizing mRNA markers of HBB, MMP10, DKK4, AKAP4 and HTN3 genes. The detection method can realize accurate traceability of the sample and effective detection of the mixed body fluid, is short in detection time, can complete the test only in 40 minutes, is high in sensitivity, simple and convenient to operate and high in body fluid traceability efficiency, gets rid of dependence on large-scale equipment, and is beneficial to popularization and application in primary forensic DNA laboratories and biological research laboratories.

The present invention discloses a hybrid DNA probability typing method for MH sequencing data. The method comprises the following steps: step 1: obtaining a priori parameters according to MH sequencing data of DNA from a single individual; step 2: obtaining characteristic information of MH sequencing data of a mixed DNA sample to be tested; step 3: filtering out noise sequences according to the sequencing information; step 4: constructing likelihood functions respectively under the prosecution hypothesis and the defense hypothesis according to the priori parameters and characteristic information, and calculating the likelihood ratio of the prosecution hypothesis and the defense hypothesis; step 5: sorting possible genotype combinations according to the value of the likelihood function; the present invention is used for the detection of common mixed DNA samples in forensic DNA analysis work, and can objectively and accurately analyze the results of MH sequencing data of mixed DNA, effectively reducing the uncertainty and subjective errors in the analysis process, and significantly improving the accuracy and scientificity of MH-MPS data analysis of forensic mixed DNA.

The invention discloses a high-performance autosome Multi-Indel genetic marker system as well as a detection primer group and a kit thereof, and belongs to the technical field of forensic medicine identification. The genetic marker system comprises 11 Multi-Indel loci, in addition, the invention also provides a primer for detecting the genetic marker system, and the sequence of the primer is as shown in SEQ ID NO.1-33. The invention also provides a kit for detecting the genetic marker system. According to the invention, a multi-PCR amplification technology and a capillary electrophoresis (CE) technology are combined, and a CE-based high-efficiency Multi-indels for forensic DNA analysis is constructed. The potential of the panel in forensic application is comprehensively explored, including sensitivity, species specificity, polymorphism, forensic parameters, degraded samples and mixed samples.

The present invention discloses a multiplex amplification system for forensic human DNA detection by qPCR and its application. The multiplex amplification system simultaneously amplifies four targets, namely HS, HL, HY and IPC, by qPCR. Among them, the HS target is used for total DNA quantification, the HL target is used for degradation degree evaluation, the HY target is used for sex ratio judgment, and the IPC target is used for inhibitor detection. The kit of the present invention detects four targets, namely HS, HL, HY and IPC, by qPCR technology to achieve DNA quantification and quality analysis, and the operation is simple. It has high sensitivity (the lowest detection limit is 1 pg / μL), strong anti-inhibition ability, good specificity, can accurately quantify male and female DNA, can also evaluate the DNA degradation degree, judge PCR inhibitors, is applicable to a variety of biological samples, provides an efficient and accurate solution for forensic DNA detection, and helps solve cases.

The present disclosure relates, generally, to nucleotide sequence data and, more particularly, to computer files and methods supporting forensic DNA analysis. In one illustrative embodiment, a method may comprise identifying a locus corresponding to each item of short tandem repeat (STR) profiling data stored in an existing computer file, wherein the STR profiling data stored in the existing computer file is repeat-based and / or length-based; identifying start and stop coordinates of an STR region of the corresponding locus for each item of STR profiling data stored in the existing computer file; creating an ambiguous text string corresponding to each item of STR profiling data stored in the existing computer file, wherein each ambiguous text string consists of a sequence of ambiguous characters extending from the start coordinate to the stop coordinate identified for the corresponding item of STR profiling data; and storing each ambiguous text string in a sequence-based computer file.

According to the full-automatic forensic DNA detection material treatment, extraction and detection integrated detection platform, a treatment reagent can be prepared through the reagent preparation equipment, the DNA detection material is treated through the detection material treatment equipment by adopting the treatment reagent, and the volume of liquid in a TIP head and the position of liquid in a consumable material are recognized through the visual recognition equipment; extraction and purification are carried out through DNA extraction and purification equipment, analysis is carried out through a nucleic acid amplification instrument and a real-time fluorescent quantitative QPCR analyzer, detection is achieved, consumable transfer among all the cabins is completed through respective consumable left and right transfer equipment, consumable transfer in all the cabins is achieved through respective mechanical arms, and the consumable transfer efficiency is improved. A user only needs to load a sample in the front, place consumables and set experimental parameters. According to the invention, full-process automation of DNA detection material treatment, extraction and detection can be realized, manual intervention is reduced, risks of operator infection, sample pollution and influence of manual misoperation on a detection result are reduced, and the accuracy of the detection result is improved.

1. Name of the product in this design: Fully Automated Forensic DNA Testing Integrated Detection Platform. 2. Purpose of this design: To realize a fully automated integrated forensic DNA testing platform. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention provides a blood separation device for forensic DNA extraction and a method thereof. The device comprises a separation box body, a liquid medicine tank, a rotating cavity and a separation driving mechanism, a rotating cavity is rotationally connected to the middle, close to the top end, in the separation box body, a plurality of test tube containing cavities are formed in the rotating cavity, test tubes containing blood are placed in the test tube containing cavities, liquid medicine tanks are further installed on the separation box body, and a separation driving mechanism is further installed at the position, below the rotating cavity, in the separation box body. The separation driving mechanism comprises a driving motor, a planetary gear, a first electromagnetic clutch, a driving shaft, an eccentric cycloid mechanism, an eccentric shaft and a second electromagnetic clutch. Through cooperation of the first electromagnetic clutch, the second electromagnetic clutch and the hydraulic rod, a blood sample in a test tube and liquid medicine are subjected to premixing treatment, uniform mixing treatment and high-speed centrifugal rotation treatment, blood separation operation can be completed conveniently and efficiently, and popularization and application are facilitated.

The present disclosure relates, generally, to nucleotide sequence data and, more particularly, to computer files and methods supporting forensic DNA analysis. In one illustrative embodiment, a method may comprise identifying a locus corresponding to each item of short tandem repeat (STR) profiling data stored in an existing computer file, wherein the STR profiling data stored in the existing computer file is repeat-based and / or length-based; identifying start and stop coordinates of an STR region of the corresponding locus for each item of STR profiling data stored in the existing computer file; creating an ambiguous text string corresponding to each item of STR profiling data stored in the existing computer file, wherein each ambiguous text string consists of a sequence of ambiguous characters extending from the start coordinate to the stop coordinate identified for the corresponding item of STR profiling data; and storing each ambiguous text string in a sequence-based computer file.