The application discloses a
human DNA sample quantitative amplification
system and application thereof, and the amplification
system comprises a primer and probe compound for specifically amplifying four target sequences; wherein the four target sequences are two autosomal target genes CSF1PO and TPOX, one
Y chromosome target gene SRY and one non-human synthetic
oligonucleotide IPC. Advantages are as follows: (1) the amplification
system and the kit thereof can be applied to all
forensic DNA analysis, four different target genes can be simultaneously amplified in one tube, human total
DNA and human male
DNA can be quantitatively and qualitatively evaluated, and information about sample degradation and inhibitor condition is provided. The primer and probe are high in specificity and sensitivity, and the minimum
detection limit can reach 1 pg level. (2) the kit effectively simplifies the steps of qPCR reaction operation, improves the
accuracy and precision of the test, and is suitable for all collected sample sources in
forensic DNA analysis.