36 results about "Vaginal secretion" patented technology

The invention discloses lactobacillus mucilaginosus and application thereof in fermentation of sporoderm-broken ganoderma lucidum spore powder. The preservation number of the lactobacillus mucilaginosus is CGMCC (China General Microbiological Culture Collection Center) No.31453. The strain is separated and screened from vaginal secretions of healthy women at the childbearing age, is not hemolyzed, is sensitive to various common antibiotics and has strong lactic acid production capacity, and fermented supernate of the strain has strong growth inhibition capacity on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, salmonella typhimurium, shigella dysenteriae and gardnerella vaginalis and can be used for producing lactic acid. The compound has strong adhesion ability, growth inhibition ability and apoptosis promoting effect on human cervical cancer cells. When the strain is used for fermenting wall-broken ganoderma lucidum spore powder, the content of bioactive substances such as amino acids and derivatives thereof, fatty acids and steroids in fermentation liquor can be greatly increased, the strain has an obvious anti-inflammatory effect on DSS-induced mouse colitis, and the inflammatory factor level is remarkably reduced. The strain not only has significant application value in functional food and biological agents, but also opens up a new way for further development of ganoderma spores.

The invention discloses a method and system for recognizing the number of bacillus in a gynecological micro-ecological microscopic image, and belongs to the technical field of image recognition and micro-ecological analysis. Performing image enhancement processing on the image to improve the definition of the target area; pixel-level segmentation is carried out on the enhanced image based on the trained image segmentation model, and a suspected bacillus target area set is extracted; extracting a morphological characteristic parameter set for each target area; screening the suspected areas by combining a bacillus morphological feature discrimination model obtained by clinical labeling sample training, and removing false targets; counting the number of the effective areas, estimating the number of bacilli in the adhesion areas by adopting a skeleton endpoint analysis method, and finally outputting the total number of bacilli in the image; the method has the characteristics of high precision, high robustness and high automation degree, and is suitable for intelligent identification of the micro-ecological structure in the gynecological microscopic image.

According to the probiotic composition and the application of the probiotic composition in preparation of products for preventing and treating vagina inflammation, the probiotic composition composed of lactobacillus gasseri B13.52 and lactobacillus mucus B14.46 screened from vaginal secretion of healthy women can effectively inhibit growth of escherichia coli, gardnerella and prevotella; the secretion of proinflammatory factors in vaginal epithelial cells can be obviously reduced, so that an anti-inflammatory effect is achieved.

The invention discloses a strain of Shenjing pig combined lactobacillus and application thereof. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.31452. The strain is separated and screened from vaginal secretions of healthy women at the childbearing age and is sensitive to various common antibiotics, and fermented supernate of the strain shows relatively strong inhibition capability on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, salmonella typhimurium, salmonella paratyphi B and gardnerella vaginalis; the compound has strong adhesion ability, growth inhibition ability and apoptosis promoting effect on human cervical cancer cells. When the strain is used for fermenting wall-broken ganoderma lucidum spore powder, the content of amino acid and bioactive substances such as peptides, arachidonic acids, steroids and polyketones can be greatly increased, the strain has an obvious inhibition effect on DSS-induced mouse colitis, and the inflammatory factor level is remarkably reduced. The strain not only has significant application value in biological agents, but also opens up a new way for combined application of lactic acid bacteria and ganoderma spores.

The invention discloses a lactobacillus jensenii strain and application thereof in fermentation of wall-broken ganoderma lucidum spore powder. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.31484. The strain is separated and screened from vaginal secretions of healthy women at the childbearing age, is sensitive to various common antibiotics, and has strong lactic acid production capacity; a fermentation supernatant of the bacillus subtilis shows relatively strong inhibition capability on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, salmonella typhimurium, shigella dysenteriae, yersinia enterocolitica and gardnerella vaginalis, and has relatively strong adhesion capability, growth inhibition capability and apoptosis promotion effect on human cervical cancer cells. When the strain is used for fermenting wall-broken ganoderma lucidum spore powder, the content of amino acid and bioactive substances such as peptides, arachidonic acids, steroids and polyketones can be greatly increased, the strain has an obvious inhibition effect on DSS-induced mouse colitis, and the inflammatory factor level is remarkably reduced. The strain not only has significant application value in biological agents, but also opens up a new way for combined application of lactic acid bacteria and ganoderma spores.

The embodiment of the invention provides a female vaginal flora structure typing method, device and equipment based on a consensus matrix and a storage medium, and relates to the technical field of biological detection.The method comprises the steps that based on extracted DNA of a plurality of female vaginal secretion swabs, PCR amplification is conducted through a 16s rRNA primer, sequencing and clustering are conducted on each amplified DNA, and the DNA of the female vaginal secretion swabs is obtained; the method comprises the following steps: generating ASV representative sequences, classifying and annotating the generated ASV representative sequences to obtain relative abundance matrix data of female vaginal flora, repeatedly sampling samples and strains of the relative abundance matrix data according to a set proportion, and clustering a plurality of generated subsets by adopting a plurality of clustering methods to obtain a plurality of clustering results; adopting a consensus matrix method to obtain a co-occurrence rate of any two samples in the relative abundance matrix data in a plurality of clustering results; all the obtained co-occurrence rates are clustered, and a flora CST typing result is obtained, so that the robustness and accuracy of female vagina flora structure typing are improved.

The invention provides a vaginal secretion sampling and storing device, and relates to the field of medical instruments. A sampling tube cap is inserted into the upper part of the sample storage test tube; a sampling cotton swab is fixedly connected to the center of the bottom end surface of the sampling tube cap; the sampling cotton swab is arranged in the sample storage test tube; the bottom end surface of the sampling cotton swab is fixedly connected with a cotton swab head; the cotton swab head is arranged in the sample storage test tube; two storage bins are symmetrically formed in the lower part of the sample storage test tube; the two storage bins are aligned with the positions of the cotton swab heads; and the top end surface of the sampling tube cap is rotationally connected with a control knob. A user only needs to rotate a control knob, insert a sampling tube cap into a sample storage test tube, and then extrude a moisturizing block through a series of transmission, so that cell preservation liquid flows out of a storage bin to infiltrate a vaginal secretion sample, and the problems that once sampling time is too long, the sample is easy to dry, the biological activity of the sample is affected, resampling is needed, and the sampling time is short are solved. And the operation is relatively tedious.

The utility model discloses an AIE vaginal secretion dual fluorescent staining solution preparation sampling tube, which relates to the field of gynecological pathogen detection, and comprises a sampling tube body, pressing holes are arranged below two sides of the sampling tube body, and shielding components are arranged on two sides inside the sampling tube body. By arranging the shielding assembly, when the negative pressure ball needs to be pressed through the pressing hole, a worker pulls a pull rope through a pull ring, the pull rope pulls a sliding block to move upwards, the sliding block moves upwards to extrude a spring, so that the spring is stressed and compressed, meanwhile, a baffle is driven to move upwards, the baffle moves into the containing cavity, and at the moment, the baffle does not shield the pressing hole any more; a worker can press the negative pressure ball through the pressing hole, when the negative pressure ball does not need to be pressed, the pull ring is loosened, the spring is stretched to push the sliding block to move downwards to reset, the baffle is driven to move to reset, the baffle shields the pressing hole, and the situation that the sealing plug is moved out of the suction pipe when the negative pressure ball is pressed by fingers in the sampling pipe taking process is avoided.

The invention provides lactobacillus rhamnosus and application thereof. The lactobacillus rhamnosus is an original strain separated and purified from vaginal secretion of healthy women. The lactobacillus rhamnosus is identified by the China General Microbiological Culture Collection Center (CGMCC) and is classified and named as lactobacillus rhamnosus, and the preservation number is CGMCC No.33918. According to the lactobacillus rhamnosus disclosed by the invention, through optimization of growth conditions, a large amount of efficient antibacterial substances such as L-lactic acid and H2O2 can be generated in a fermentation culture solution of the lactobacillus rhamnosus, and the growth of gardneria vaginalis can be effectively inhibited. The lactobacillus rhamnosus disclosed by the invention is a microorganism capable of efficiently inhibiting gardneria vaginalis and relieving bacterial vaginosis of mice, and has a relatively high application value.

The application discloses a composite amplification detection system for body fluid tracing based on mRNA markers and application thereof, high-specificity mRNA markers can realize accurate tracing of five main body fluid types including peripheral blood, saliva, semen, menstrual blood and vaginal secretion, and can make up for the problem that in the previous body fluid tracing system, less markers lead to false positive or false negative problems caused by cross-reaction, and effectively improve the accuracy of single source and mixed body fluid, and old body fluid identification, and improve the applicability and feasibility of the system in forensic practice and biological research. Based on the fluorescence labeling technology, the application realizes stable, specific and efficient composite amplification reaction of multiple high-specificity mRNA markers at the same time, and the number of composite mRNA markers in the system is relatively large, and the identification efficiency of single source and mixed body fluid, and old body fluid is high.

The gynecological uterine cavity sampler comprises a sampling tube and a sampling hole formed in the end part of the sampling tube, and further comprises a piston, and the piston is arranged in the sampling tube; one end of the push-pull rod is positioned in the sampling tube and is fixed with the piston; the plugging assembly is arranged on the sampling tube, the plugging assembly is used for plugging the sampling hole, the sampling tube is moved into the uterine cavity, the plugging assembly is used for removing plugging, and the sampling hole is temporarily plugged through the sliding sleeve, so that when a medical worker uses the sampling tube to penetrate through the vagina of a patient, the plugging assembly is used for plugging the sampling hole and moving the sampling tube into the uterine cavity; the condition that secreta in the vagina enters the sampling tube from the sampling hole in the uterine cavity penetrating process is avoided, then the sampling purity is improved, the detection accuracy is improved, when the sampling hole is located in the uterine cavity of a patient, the handle is pulled to drive the arc-shaped piece to move, and therefore the sliding sleeve is driven to move to open the sampling hole, and the sampling tube is closed. And meanwhile, sampling operation is performed.

Provided is a product that enables easy monitoring of early rupture of membranes and relates to a wearing article provided with a test strip using immunochromatography, wherein the test strip is designed to detect amniotic fluid-derived insulin-like growth factor binding protein 1 in vaginal secretion, and wherein, when the test strip detects 0.3-25 mg / L of insulin-like growth factor binding protein 1, the result is considered positive.

The invention relates to the technical field of medical instruments, in particular to a vaginal lubricant and a preparation method thereof.The vaginal lubricant is prepared from, by mass, 0.05-0.5 part of tremella polysaccharide and 0.1-1.5 parts of soluble proteoglycan, all the ingredients are odorless, mild in property, free of vagina irritation and free of adverse reaction on the human body, and the vaginal lubricant is free of side effects on the human body. The tremella polysaccharide with the molecular weight of 1000000-150000 Da and the soluble proteoglycan with the molecular weight of 400000-600000 Da are adopted for compatibility, so that vaginal secretion can be fully absorbed, and the imaging effect of colposcopy is improved; the vaginal dilator has the advantages that the vaginal dilator is convenient to use, vagina injury caused by cervical forceps and other instruments can be quickly repaired, the elasticity of the dilated vagina can be quickly recovered, good lubricity is achieved, friction force between the instruments and a cavity can be reduced to a great extent, and mechanical injury is effectively avoided.

The invention relates to the technical field of medical apparatus and instruments, and particularly discloses an anti-pollution female perineal area urine collection device which comprises an isolation cover, a first liquid outlet hole and a second liquid outlet hole are formed in the outer surface of the upper end of the isolation cover in a penetrating mode, a rotating pipe is rotatably connected to the outer surface of the lower end of the first liquid outlet hole, and a connecting pipe is rotatably connected to the outer surface of the rotating pipe. A reducer pipe is fixedly connected to the lower end of the connecting pipe, a flow limiting assembly is arranged on the inner side of the connecting pipe and comprises a sealing plate fixedly connected with the inner surface of the connecting pipe, and a guide rod is fixedly connected to the outer surface of the upper end of the sealing plate. Through the arrangement of the separation assembly and the liquid absorption cotton, redundant liquid can be absorbed, so that the first liquid outlet hole and the second liquid outlet hole can be physically sealed, the probability that vaginal secretions are mixed with urine can be effectively reduced, the leaked urine can be absorbed through the protective edge, and the leakage of the urine is avoided. Therefore, the sealing effect of the urine collecting device can be further improved.

The utility model discloses a vaginal secretion collector relates to medical equipment technical field. Prior art adopts traditional glass tube or plastic tube as sampling tube, and it is easy to cause sample leakage. The utility model's sampling tube inside preset physiological saline, and sampling tube is set up elastic diaphragm self -sealed structure relative to physiological saline top, and sampling tube top detachable connecting pipe cover, and the quick sample storage cover is rotatably arranged on the pipe cover, and the elastic diaphragm self -sealed structure is directly passed through by the quick sample storage cover, and the elastic diaphragm self -sealed structure is symmetrically set up the intercommunication trapezoidal round hole on the two sides of center respectively, and the sampling cotton swab is convenient, and the trapezoidal round hole is quickly passed through through the sample storage hole of pipe cover when using, and then closes quick sample storage cover can seal. The utility model not only can guarantee sampling result accuracy, improve sampling efficiency, but also improve its sealing performance through multilayer sealing design, has very high practicality.

The invention discloses a detection method for forensic body fluid traceability. According to the method, peripheral blood, menstrual blood, vaginal secretion, semen and saliva are detected by using an RPA-CRISPR-Cas12a-LFD detection system and utilizing mRNA markers of HBB, MMP10, DKK4, AKAP4 and HTN3 genes. The detection method can realize accurate traceability of the sample and effective detection of the mixed body fluid, is short in detection time, can complete the test only in 40 minutes, is high in sensitivity, simple and convenient to operate and high in body fluid traceability efficiency, gets rid of dependence on large-scale equipment, and is beneficial to popularization and application in primary forensic DNA laboratories and biological research laboratories.

The invention relates to a capsule for treating gynecological diseases and a preparation method thereof. The capsule is prepared from polygonum cuspidatum, sargentgloryvine stem, coptis chinensis, rhizoma bletillae, radix gentianae, dragon's blood, cuttlebone, rheum officinale, aloe, catechu, astragalus membranaceus, chloramphenicol and purified water. The capsule is prepared through a powder preparation process, a conventional vaginal medicine filling method is adopted for administration, the capsule is dissolved by virtue of vaginal secretion to release medicine, and the medicine acts on the vagina, the cervix uteri and the endometrium and directly reaches a focus, so that the effects of diminishing inflammation, sterilizing, resisting viruses, diminishing swelling, removing stasis and repairing mucosa are achieved; the traditional Chinese medicine composition can be used for treating colpitis mycotica, infectious microbe vaginitis, cervical erosion, cervicitis, HPV infection and endometritis. The capsule is a proved recipe inherited by several generations, has a good curative effect, takes traditional Chinese medicines as main raw materials, is added with a small amount of western medicines, has the advantages of benefiting and eliminating disadvantages, has no side effect, no preservative and no irritation, has an effective rate of 99% and a cure rate of 98%, and is worthy of popularization.

The invention relates to a multiplex fluorescent quantitative PCR primer probe combination for detecting common pathogenic bacteria in vaginal secretion and application of the multiplex fluorescent quantitative PCR primer probe combination, and belongs to the technical field of biology. The primer probe combination provided by the invention contains any one of primer probe groups 1-7. A specific primer probe combination is designed for a cfb gene and a dits gene of streptococcus agalactiae, a sapB2 gene and a cadF gene of campylobacter fetus, a nuc gene of staphylococcus aureus, an hlyA gene of listeria monocytogenes and a ureaplasma ure gene, and a multiplex fluorescent quantitative PCR detection kit and method are established and used for screening common neonatal pathogenic bacteria carried in the birth canal of a puerpera. Seven pathogenic bacteria can be detected at the same time, the specificity is 100%, the sensitivity reaches 85% or above, the minimum detection limit reaches the femtogram level, clinical intervention and early intervention can be fed back in time, and the situation of neonatal infection is avoided.

The invention discloses a vagina micro-ecology automatic detection system and method based on immunofluorescence microscopic scanning and AI analysis. The system comprises a fluorescence imaging module, a motion control module, an image processing module and an AI intelligent analysis module. The motion control module is used for bearing a vaginal secretion slide sample and controlling the vaginal secretion slide sample to move in a three-dimensional space, and is matched with the fluorescence imaging module to carry out full-automatic scanning; the image processing module is used for enhancing and de-noising the collected original fluorescence image so as to improve the image quality; the AI analysis module performs multi-target recognition and quantitative analysis on the processed image based on a deep learning algorithm, and counts the number and proportion of various bacteria and cells; and finally, automatically generating a micro-ecology assessment report according to clinical rules. The full-flow automatic and intelligent detection of the vaginal secretion sample is realized, the problems of strong subjectivity and low efficiency of manual microscopic examination are effectively solved, and higher sensitivity, specificity and repeatability in vaginitis diagnosis are realized.

The application discloses a kind of based on attention mechanism and multi-scale fusion's vaginitis pathogen cell detection method and system.The method will be obtained in advance vaginal secretion immunofluorescence microscopic image input training completed optimal pathogen detection model, output the detection result of recognition trichomonad, leukocyte, epithelial cell, clue cell and spore.The application is based on deep learning algorithm to realize the automatic high-efficiency accurate detection of vaginitis pathogen cell, improve the confirmed diagnosis rate of vaginitis, greatly reduce the probability of missed detection, misdiagnosis phenomenon in diagnosis process.According to pathogen cell detection result, simulate clinical diagnosis output, realize pathogen cell classification judgment, provide reliable basis for the automation auxiliary diagnosis of vaginitis.

This invention belongs to the field of biomedicine and discloses the application of HMOX1 inhibitors in the preparation of drugs for treating senile vaginitis. This invention is the first to discover that the content of the metabolite MESV1 (Guggulsterone) is significantly elevated in the vaginal secretions of patients with senile vaginitis. MESV1 induces ferroptosis in vaginal epithelial cells by upregulating HMOX1 protein expression, thereby driving the disease. Based on this, this invention proposes a novel therapeutic strategy targeting HMOX1. The inhibitors include shRNAs targeting HMOX1 (such as SEQ ID NO: 1-3), which can be prepared into vaginal gels, creams, and other dosage forms. Simultaneously, MESV1 can also be used to prepare auxiliary diagnostic kits. This invention provides a novel target and precise treatment approach for non-hormonal treatment of senile vaginitis.

The application discloses a composition for preventing and / or treating candidal vaginitis, and belongs to the field of medicine. It is found for the first time that a composition containing volatile oil of pachystachys jutna and total alkaloids of Sophora flavescens can significantly inhibit the adhesion and invasion ability of Candida albicans to VK2 / E6E7 cells. Meanwhile, in a mouse candidal vaginitis model, the composition can effectively reduce the vaginal fungal load of the mouse candidal vaginitis model, improve the pH value of vaginal secretion, and improve the inflammatory cell infiltration of the vaginal tissue, and has clinical popularization and application value.

The invention provides a quality control product for detecting vaginal secretion and a preparation method of the quality control product. The quality control product comprises a negative quality control product and a positive quality control product; the positive quality control product comprises 20-200 mM of a second basic buffer solution, 1-5% of a second enzyme protective agent, 0-0.04% of lactic acid, 0-0.005% of hydrogen peroxide, 0.01-0.1 U / mL of a second leukocyte esterase item, 0.02-0.1 U / mL of sialidase, 0.05-0.5 U / mL of a second N-acetylglucosaminidase item, 50-200 U / mL of a second oxidase item, 2-40 U / mL of a second coagulase item, 50-200 U / mL of beta-glucuronidase, 5-20 U / mL of proline aminopeptidase, 1-5 U / mL of a second alkaline phosphatase item and 0.2-0.2. And 1% of bovine serum albumin.

The invention relates to the technical field of medical apparatus and instruments, and discloses a urine sample collection device for a clinical laboratory, the urine sample collection device comprises a supporting bottom plate, the middle part of the upper surface of the supporting bottom plate is fixedly connected with a collection block, and the collection block is internally provided with an adjusting assembly. The driving motor is started to drive the second rotating shaft to rotate, and after the second rotating shaft rotates, the sampling support plate presents an inclined angle through a series of transmission, so that the radian of urine is controlled, the initial horizontal speed of the urine can be inhibited in the sampling process, and the sampling accuracy is improved. Skin debris, clothing fibers or environmental pollutants are prevented from entering a sample due to splashing, meanwhile, a vertical drop point can be optimized during female sampling, the contact between a urethral orifice and the inner wall of a container is reduced, the risk of mixing vaginal secretion or vulva pollutants is reduced, and when elderly patients are sampled, the sampling time is shortened, and the sampling efficiency is improved. Therefore, cross infection and health risks caused by the problems of vision decline and hand flexibility weakening of the elderly patients can be avoided.

The application discloses Lactobacillus gasseri and a composition thereof for simultaneously improving female private health and skin aging, and belongs to the technical field of microorganisms.The application screens a Lactobacillus gasseri CCFM1313 from vaginal secretions of healthy women, the Lactobacillus gasseri CCFM1313 and the composition containing the Lactobacillus gasseri CCFM1313 and Paracaseiclovor CCFM1316 or a fermentation liquor thereof have the effects of repairing oxidative damage related symptoms caused by vaginitis and relieving skin aging.Therefore, the Lactobacillus gasseri CCFM1313 and the composition containing the Lactobacillus gasseri CCFM1313 and Paracaseiclovor CCFM1316 or the fermentation liquor thereof have great application prospects in products for improving and repairing oxidative damage caused by vaginitis and skin health problems caused by vaginitis.

The invention discloses a multiplex amplification detection system for body fluid traceability based on mRNA markers and application of the multiplex amplification detection system, and high-specificity mRNA markers can realize accurate traceability of five main body fluid types including peripheral blood, saliva, semen, menstrual blood and vaginal secretion. The method solves the problem that false positive or false negative caused by cross reaction cannot be interpreted due to few marks in the conventional body fluid traceability system, effectively improves the identification accuracy of single-source and mixed body fluid and old body fluid, and improves the applicability and feasibility of the system in forensic practice and biological research. Based on a fluorescence labeling technology, stable, specific and efficient composite amplification reaction for a plurality of high-specificity mRNA markers is realized at the same time, the number of composite mRNA markers in the system is large, and the identification efficiency for single-source mixed body fluid and old body fluid is high.

The invention relates to the technical field of gynecological medical instruments, in particular to a vaginal secretion sampling device which comprises an intubation tube, a sampling assembly, a plurality of expansion assemblies, a plurality of push plates, a limiting frame and an adjusting assembly. One end of the intubation tube is open, and the sampling assembly sleeves the outer surface of the intubation tube. The plurality of expansion assemblies are arranged in the insertion pipe, and the plurality of push plates are arranged between the insertion pipe and the sampling assembly. The limiting frame is fixedly arranged at the open end of the insertion pipe and comprises a guide cylinder. The adjusting assembly comprises a position adjusting rod, the position adjusting rod is arranged in the guide cylinder and can slide along the inner wall of the guide cylinder, an ejector rod is fixedly arranged at the end, close to the insertion pipe, of the position adjusting rod, and the central axis of the guide cylinder, the central axis of the position adjusting rod and the central axis of the ejector rod are collinear with the central axis of the insertion pipe. When the device is used, the guide cylinder can limit the position adjusting rod to move along the central axis of the cannula all the time, the ejector rod cannot incline, and the sampling assembly can be uniformly stretched, so that the normal sampling process can be ensured, and the comfort of a female patient is improved.

The invention discloses a method for identifying menstrual blood and peripheral blood by using an expression quantity relationship of miRNA based on RNU6b as a reference gene, and relates to the technical field of biology. According to the invention, three miRNAs (miR-144-5p, miR-203-3p and miR-451a) with high expression difference are screened from vaginal blood and peripheral blood on the basis of a high-throughput sequencing technology for the first time, and the difference of screening indexes is further verified in other three body fluids (semen, saliva and vaginal secretion); meanwhile, six reference genes (RNU6b, U6, miR-103a-3p, miR-191-5p, miR-92a-3p and miR-484) are screened to evaluate comparison results, and the RNU6b with the highest stability is finally selected as the reference gene, so that an efficient and accurate identification method system is finally established, or the method will become a powerful tool for body fluid plaque tissue sources of forensic physical evidence, and can be used as a kit for identifying body fluid plaque tissue sources of forensic physical evidence. And more scientific and reasonable auxiliary evidences are provided for qualitative and detection of cases.