46 results about "Vaginal secretion" patented technology

The invention discloses a multiple primer group for detecting pathogens of reproductive system diseases and a kit thereof. The kit comprises a combination of amplification primers and fluorescent probes of 10 pathogens, the pathogens comprise molluscum contagiosum virus, Snethia bacteria, gardnerella vaginalis, prevotella genus, campylobacter genus, giant globules genus, Atortobacter vaginalis, lactobacillus jensenii, lactobacillus crispatus and inert lactobacillus. The kit contains UDG enzyme and is used for preventing pollution of a PCR amplification product, and the accuracy of a detection result is ensured. The kit provided by the invention contains an internal reference control, can display the occurrence of false negative, and is used for monitoring the collection, transportation, extraction and amplification processes of a to-be-detected sample. According to the kit, the specificity is 100%, the detection sensitivity reaches 10 copies / mL, the specificity is good, and the detection efficiency is high. The kit is used for identifying the reproductive system disease pathogens of a to-be-detected sample, the to-be-detected sample can be vaginal secretion, the operation steps are simple, and the detection period is short.

The invention discloses lactobacillus mucilaginosus and application thereof in fermentation of sporoderm-broken ganoderma lucidum spore powder. The preservation number of the lactobacillus mucilaginosus is CGMCC (China General Microbiological Culture Collection Center) No.31453. The strain is separated and screened from vaginal secretions of healthy women at the childbearing age, is not hemolyzed, is sensitive to various common antibiotics and has strong lactic acid production capacity, and fermented supernate of the strain has strong growth inhibition capacity on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, salmonella typhimurium, shigella dysenteriae and gardnerella vaginalis and can be used for producing lactic acid. The compound has strong adhesion ability, growth inhibition ability and apoptosis promoting effect on human cervical cancer cells. When the strain is used for fermenting wall-broken ganoderma lucidum spore powder, the content of bioactive substances such as amino acids and derivatives thereof, fatty acids and steroids in fermentation liquor can be greatly increased, the strain has an obvious anti-inflammatory effect on DSS-induced mouse colitis, and the inflammatory factor level is remarkably reduced. The strain not only has significant application value in functional food and biological agents, but also opens up a new way for further development of ganoderma spores.

The invention discloses a method and system for recognizing the number of bacillus in a gynecological micro-ecological microscopic image, and belongs to the technical field of image recognition and micro-ecological analysis. Performing image enhancement processing on the image to improve the definition of the target area; pixel-level segmentation is carried out on the enhanced image based on the trained image segmentation model, and a suspected bacillus target area set is extracted; extracting a morphological characteristic parameter set for each target area; screening the suspected areas by combining a bacillus morphological feature discrimination model obtained by clinical labeling sample training, and removing false targets; counting the number of the effective areas, estimating the number of bacilli in the adhesion areas by adopting a skeleton endpoint analysis method, and finally outputting the total number of bacilli in the image; the method has the characteristics of high precision, high robustness and high automation degree, and is suitable for intelligent identification of the micro-ecological structure in the gynecological microscopic image.

The invention relates to a composite amplification detection system for identifying human skin based on microbial markers. The multiplex amplification detection system comprises a primer combination for specifically amplifying five human skin microorganism markers, wherein the human skin microorganism markers are staphylococcus epidermidis, staphylococcus hominis, staphylococcus capitis, staphylococcus aureus and propionibacterium acnes respectively. The invention also provides a kit containing the composite amplification detection system. The multiplex amplification detection system and the kit have relatively good recognition capability on skin of parts such as forehead, cheeks, hands, chest, navel periphery and groin of a human body. Besides, the method is an effective supplement for an existing body fluid identification method, and can effectively identify various common body fluid samples in forensic cases by flexibly combining with a multiple amplification system for saliva and vaginal secretion established by a previous team, so that the accuracy and reliability of identification results are improved, and case investigation and judicial trial are assisted.

The invention discloses lactobacillus crispatus for improving female genital tract microenvironment and immune aging and application of the lactobacillus crispatus, and belongs to the field of probiotics. The probiotic lactobacillus crispatus ProSci-38 disclosed by the invention can be used for effectively improving the symptoms such as pruritus vulvae, causalgia of vagina, abnormal vaginal secretion and the like of patients with vaginitis; mainly through the effect of intestinal tract-vaginal axis, the proportion of lactobacillus is increased, the proportion of harmful bacteria is reduced, the balance of vaginal flora is recovered, and the acidic environment of vagina is maintained; meanwhile, the inflammatory factor level can be reduced, the immune function of the vagina of a patient can be improved, so that the vagina environment is improved, the infection risk is reduced, and the preparation method has a great prospect in preparation of products for relieving and / or treating vaginitis.

The invention discloses a kit for vaginal microorganism fluorescence detection and a preparation method thereof, and belongs to the technical field of pathogenic microorganism fluorescence detection in medical clinical examination. The kit provided by the invention comprises a liquid A and a liquid B, the solution A comprises a fluorescent staining agent, a bacteriostatic agent, an anti-fluorescence decline agent, a counterstaining agent and purified water; the solution B comprises fluorescein, a buffer solution, a preservative, a stabilizer and purified water. According to the application, the liquid A and the liquid B are combined into the kit, pathogens in vaginal secretion can be marked, and the inflammation type can be judged more comprehensively and accurately, so that the whole kit is more accurate and sensitive.

According to the probiotic composition and the application of the probiotic composition in preparation of products for preventing and treating vagina inflammation, the probiotic composition composed of lactobacillus gasseri B13.52 and lactobacillus mucus B14.46 screened from vaginal secretion of healthy women can effectively inhibit growth of escherichia coli, gardnerella and prevotella; the secretion of proinflammatory factors in vaginal epithelial cells can be obviously reduced, so that an anti-inflammatory effect is achieved.

The invention discloses a gynecological clinical vaginal secretion sampling device, and relates to the technical field of medical instruments, the gynecological clinical vaginal secretion sampling device comprises at least two independent sampling devices, every two adjacent independent sampling devices are in vertical sliding connection, and each independent sampling device comprises a protective shell with a cavity structure; the sampling mechanism penetrates through the protective shell and comprises a manual operation part, a rotating rod vertically and upwards connected to the manual operation part and a sampling air bag connected to the top end of the rotating rod, and the sampling air bag is enlarged to be attached to the inner wall of the vagina by pushing the manual operation part upwards; the adjusting mechanism is arranged on one side of the sampling mechanism and is in transmission connection with the sampling mechanism through a transmission mechanism, so that the sampling air bag deviates towards the periphery and is used for attaching the sampling air bag to the inner wall of the vagina, and sampling is facilitated. According to the sampling device, the height and the angle of a sampling part can be flexibly adjusted, samples can be conveniently collected and stored, discomfort can be reduced to the maximum extent, and injuries can be avoided.

The invention discloses a strain of Shenjing pig combined lactobacillus and application thereof. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.31452. The strain is separated and screened from vaginal secretions of healthy women at the childbearing age and is sensitive to various common antibiotics, and fermented supernate of the strain shows relatively strong inhibition capability on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, salmonella typhimurium, salmonella paratyphi B and gardnerella vaginalis; the compound has strong adhesion ability, growth inhibition ability and apoptosis promoting effect on human cervical cancer cells. When the strain is used for fermenting wall-broken ganoderma lucidum spore powder, the content of amino acid and bioactive substances such as peptides, arachidonic acids, steroids and polyketones can be greatly increased, the strain has an obvious inhibition effect on DSS-induced mouse colitis, and the inflammatory factor level is remarkably reduced. The strain not only has significant application value in biological agents, but also opens up a new way for combined application of lactic acid bacteria and ganoderma spores.

The invention discloses a lactobacillus jensenii strain and application thereof in fermentation of wall-broken ganoderma lucidum spore powder. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.31484. The strain is separated and screened from vaginal secretions of healthy women at the childbearing age, is sensitive to various common antibiotics, and has strong lactic acid production capacity; a fermentation supernatant of the bacillus subtilis shows relatively strong inhibition capability on escherichia coli, pseudomonas aeruginosa, staphylococcus aureus, salmonella typhimurium, shigella dysenteriae, yersinia enterocolitica and gardnerella vaginalis, and has relatively strong adhesion capability, growth inhibition capability and apoptosis promotion effect on human cervical cancer cells. When the strain is used for fermenting wall-broken ganoderma lucidum spore powder, the content of amino acid and bioactive substances such as peptides, arachidonic acids, steroids and polyketones can be greatly increased, the strain has an obvious inhibition effect on DSS-induced mouse colitis, and the inflammatory factor level is remarkably reduced. The strain not only has significant application value in biological agents, but also opens up a new way for combined application of lactic acid bacteria and ganoderma spores.

The invention relates to the technical field of sampling instruments, in particular to a gynecological clinical vaginal secretion sampling device. The sampling device comprises a sampling handheld part and two bent connecting arms fixed at one end of the sampling handheld part, a reinforcing block is fixed at one ends of the two bent connecting arms, and a sampling mechanism is assembled between the sampling handheld part and the reinforcing block. Through the structural design of the sampling mechanism and the auxiliary mechanism, the device can go deep into a deeper part in the vagina when sampling vaginal secretion of a patient, so that secretion samples which are difficult to touch by a traditional method can be easily obtained, the vaginal secretion can be sucked in a mode of pressing an air blowing pipe, and the vaginal secretion sampling efficiency is improved. Vaginal secretion can be stored in the sampling cup, subsequent direct sample sending examination is facilitated, the accuracy of gynecological examination and the patient experience are greatly improved, and meanwhile, through the structural design of the limiting mechanism, the device can position the position of the center transmission rod.

The embodiment of the invention provides a female vaginal flora structure typing method, device and equipment based on a consensus matrix and a storage medium, and relates to the technical field of biological detection.The method comprises the steps that based on extracted DNA of a plurality of female vaginal secretion swabs, PCR amplification is conducted through a 16s rRNA primer, sequencing and clustering are conducted on each amplified DNA, and the DNA of the female vaginal secretion swabs is obtained; the method comprises the following steps: generating ASV representative sequences, classifying and annotating the generated ASV representative sequences to obtain relative abundance matrix data of female vaginal flora, repeatedly sampling samples and strains of the relative abundance matrix data according to a set proportion, and clustering a plurality of generated subsets by adopting a plurality of clustering methods to obtain a plurality of clustering results; adopting a consensus matrix method to obtain a co-occurrence rate of any two samples in the relative abundance matrix data in a plurality of clustering results; all the obtained co-occurrence rates are clustered, and a flora CST typing result is obtained, so that the robustness and accuracy of female vagina flora structure typing are improved.

The invention provides a vaginal secretion sampling and storing device, and relates to the field of medical instruments. A sampling tube cap is inserted into the upper part of the sample storage test tube; a sampling cotton swab is fixedly connected to the center of the bottom end surface of the sampling tube cap; the sampling cotton swab is arranged in the sample storage test tube; the bottom end surface of the sampling cotton swab is fixedly connected with a cotton swab head; the cotton swab head is arranged in the sample storage test tube; two storage bins are symmetrically formed in the lower part of the sample storage test tube; the two storage bins are aligned with the positions of the cotton swab heads; and the top end surface of the sampling tube cap is rotationally connected with a control knob. A user only needs to rotate a control knob, insert a sampling tube cap into a sample storage test tube, and then extrude a moisturizing block through a series of transmission, so that cell preservation liquid flows out of a storage bin to infiltrate a vaginal secretion sample, and the problems that once sampling time is too long, the sample is easy to dry, the biological activity of the sample is affected, resampling is needed, and the sampling time is short are solved. And the operation is relatively tedious.

The utility model discloses an AIE vaginal secretion dual fluorescent staining solution preparation sampling tube, which relates to the field of gynecological pathogen detection, and comprises a sampling tube body, pressing holes are arranged below two sides of the sampling tube body, and shielding components are arranged on two sides inside the sampling tube body. By arranging the shielding assembly, when the negative pressure ball needs to be pressed through the pressing hole, a worker pulls a pull rope through a pull ring, the pull rope pulls a sliding block to move upwards, the sliding block moves upwards to extrude a spring, so that the spring is stressed and compressed, meanwhile, a baffle is driven to move upwards, the baffle moves into the containing cavity, and at the moment, the baffle does not shield the pressing hole any more; a worker can press the negative pressure ball through the pressing hole, when the negative pressure ball does not need to be pressed, the pull ring is loosened, the spring is stretched to push the sliding block to move downwards to reset, the baffle is driven to move to reset, the baffle shields the pressing hole, and the situation that the sealing plug is moved out of the suction pipe when the negative pressure ball is pressed by fingers in the sampling pipe taking process is avoided.

The invention provides lactobacillus rhamnosus and application thereof. The lactobacillus rhamnosus is an original strain separated and purified from vaginal secretion of healthy women. The lactobacillus rhamnosus is identified by the China General Microbiological Culture Collection Center (CGMCC) and is classified and named as lactobacillus rhamnosus, and the preservation number is CGMCC No.33918. According to the lactobacillus rhamnosus disclosed by the invention, through optimization of growth conditions, a large amount of efficient antibacterial substances such as L-lactic acid and H2O2 can be generated in a fermentation culture solution of the lactobacillus rhamnosus, and the growth of gardneria vaginalis can be effectively inhibited. The lactobacillus rhamnosus disclosed by the invention is a microorganism capable of efficiently inhibiting gardneria vaginalis and relieving bacterial vaginosis of mice, and has a relatively high application value.

The application discloses a composite amplification detection system for body fluid tracing based on mRNA markers and application thereof, high-specificity mRNA markers can realize accurate tracing of five main body fluid types including peripheral blood, saliva, semen, menstrual blood and vaginal secretion, and can make up for the problem that in the previous body fluid tracing system, less markers lead to false positive or false negative problems caused by cross-reaction, and effectively improve the accuracy of single source and mixed body fluid, and old body fluid identification, and improve the applicability and feasibility of the system in forensic practice and biological research. Based on the fluorescence labeling technology, the application realizes stable, specific and efficient composite amplification reaction of multiple high-specificity mRNA markers at the same time, and the number of composite mRNA markers in the system is relatively large, and the identification efficiency of single source and mixed body fluid, and old body fluid is high.

The gynecological uterine cavity sampler comprises a sampling tube and a sampling hole formed in the end part of the sampling tube, and further comprises a piston, and the piston is arranged in the sampling tube; one end of the push-pull rod is positioned in the sampling tube and is fixed with the piston; the plugging assembly is arranged on the sampling tube, the plugging assembly is used for plugging the sampling hole, the sampling tube is moved into the uterine cavity, the plugging assembly is used for removing plugging, and the sampling hole is temporarily plugged through the sliding sleeve, so that when a medical worker uses the sampling tube to penetrate through the vagina of a patient, the plugging assembly is used for plugging the sampling hole and moving the sampling tube into the uterine cavity; the condition that secreta in the vagina enters the sampling tube from the sampling hole in the uterine cavity penetrating process is avoided, then the sampling purity is improved, the detection accuracy is improved, when the sampling hole is located in the uterine cavity of a patient, the handle is pulled to drive the arc-shaped piece to move, and therefore the sliding sleeve is driven to move to open the sampling hole, and the sampling tube is closed. And meanwhile, sampling operation is performed.

Provided is a product that enables easy monitoring of early rupture of membranes and relates to a wearing article provided with a test strip using immunochromatography, wherein the test strip is designed to detect amniotic fluid-derived insulin-like growth factor binding protein 1 in vaginal secretion, and wherein, when the test strip detects 0.3-25 mg / L of insulin-like growth factor binding protein 1, the result is considered positive.

The invention relates to the technical field of medical instruments, in particular to a vaginal lubricant and a preparation method thereof.The vaginal lubricant is prepared from, by mass, 0.05-0.5 part of tremella polysaccharide and 0.1-1.5 parts of soluble proteoglycan, all the ingredients are odorless, mild in property, free of vagina irritation and free of adverse reaction on the human body, and the vaginal lubricant is free of side effects on the human body. The tremella polysaccharide with the molecular weight of 1000000-150000 Da and the soluble proteoglycan with the molecular weight of 400000-600000 Da are adopted for compatibility, so that vaginal secretion can be fully absorbed, and the imaging effect of colposcopy is improved; the vaginal dilator has the advantages that the vaginal dilator is convenient to use, vagina injury caused by cervical forceps and other instruments can be quickly repaired, the elasticity of the dilated vagina can be quickly recovered, good lubricity is achieved, friction force between the instruments and a cavity can be reduced to a great extent, and mechanical injury is effectively avoided.

The invention relates to the technical field of medical apparatus and instruments, and particularly discloses an anti-pollution female perineal area urine collection device which comprises an isolation cover, a first liquid outlet hole and a second liquid outlet hole are formed in the outer surface of the upper end of the isolation cover in a penetrating mode, a rotating pipe is rotatably connected to the outer surface of the lower end of the first liquid outlet hole, and a connecting pipe is rotatably connected to the outer surface of the rotating pipe. A reducer pipe is fixedly connected to the lower end of the connecting pipe, a flow limiting assembly is arranged on the inner side of the connecting pipe and comprises a sealing plate fixedly connected with the inner surface of the connecting pipe, and a guide rod is fixedly connected to the outer surface of the upper end of the sealing plate. Through the arrangement of the separation assembly and the liquid absorption cotton, redundant liquid can be absorbed, so that the first liquid outlet hole and the second liquid outlet hole can be physically sealed, the probability that vaginal secretions are mixed with urine can be effectively reduced, the leaked urine can be absorbed through the protective edge, and the leakage of the urine is avoided. Therefore, the sealing effect of the urine collecting device can be further improved.

The utility model discloses a vaginal secretion collector relates to medical equipment technical field. Prior art adopts traditional glass tube or plastic tube as sampling tube, and it is easy to cause sample leakage. The utility model's sampling tube inside preset physiological saline, and sampling tube is set up elastic diaphragm self -sealed structure relative to physiological saline top, and sampling tube top detachable connecting pipe cover, and the quick sample storage cover is rotatably arranged on the pipe cover, and the elastic diaphragm self -sealed structure is directly passed through by the quick sample storage cover, and the elastic diaphragm self -sealed structure is symmetrically set up the intercommunication trapezoidal round hole on the two sides of center respectively, and the sampling cotton swab is convenient, and the trapezoidal round hole is quickly passed through through the sample storage hole of pipe cover when using, and then closes quick sample storage cover can seal. The utility model not only can guarantee sampling result accuracy, improve sampling efficiency, but also improve its sealing performance through multilayer sealing design, has very high practicality.

The invention discloses a detection method for forensic body fluid traceability. According to the method, peripheral blood, menstrual blood, vaginal secretion, semen and saliva are detected by using an RPA-CRISPR-Cas12a-LFD detection system and utilizing mRNA markers of HBB, MMP10, DKK4, AKAP4 and HTN3 genes. The detection method can realize accurate traceability of the sample and effective detection of the mixed body fluid, is short in detection time, can complete the test only in 40 minutes, is high in sensitivity, simple and convenient to operate and high in body fluid traceability efficiency, gets rid of dependence on large-scale equipment, and is beneficial to popularization and application in primary forensic DNA laboratories and biological research laboratories.

The invention relates to a capsule for treating gynecological diseases and a preparation method thereof. The capsule is prepared from polygonum cuspidatum, sargentgloryvine stem, coptis chinensis, rhizoma bletillae, radix gentianae, dragon's blood, cuttlebone, rheum officinale, aloe, catechu, astragalus membranaceus, chloramphenicol and purified water. The capsule is prepared through a powder preparation process, a conventional vaginal medicine filling method is adopted for administration, the capsule is dissolved by virtue of vaginal secretion to release medicine, and the medicine acts on the vagina, the cervix uteri and the endometrium and directly reaches a focus, so that the effects of diminishing inflammation, sterilizing, resisting viruses, diminishing swelling, removing stasis and repairing mucosa are achieved; the traditional Chinese medicine composition can be used for treating colpitis mycotica, infectious microbe vaginitis, cervical erosion, cervicitis, HPV infection and endometritis. The capsule is a proved recipe inherited by several generations, has a good curative effect, takes traditional Chinese medicines as main raw materials, is added with a small amount of western medicines, has the advantages of benefiting and eliminating disadvantages, has no side effect, no preservative and no irritation, has an effective rate of 99% and a cure rate of 98%, and is worthy of popularization.

The invention relates to a multiplex fluorescent quantitative PCR primer probe combination for detecting common pathogenic bacteria in vaginal secretion and application of the multiplex fluorescent quantitative PCR primer probe combination, and belongs to the technical field of biology. The primer probe combination provided by the invention contains any one of primer probe groups 1-7. A specific primer probe combination is designed for a cfb gene and a dits gene of streptococcus agalactiae, a sapB2 gene and a cadF gene of campylobacter fetus, a nuc gene of staphylococcus aureus, an hlyA gene of listeria monocytogenes and a ureaplasma ure gene, and a multiplex fluorescent quantitative PCR detection kit and method are established and used for screening common neonatal pathogenic bacteria carried in the birth canal of a puerpera. Seven pathogenic bacteria can be detected at the same time, the specificity is 100%, the sensitivity reaches 85% or above, the minimum detection limit reaches the femtogram level, clinical intervention and early intervention can be fed back in time, and the situation of neonatal infection is avoided.

The invention discloses a preparation method of a vaginal secretion hydrogen peroxide hypersensitive detection reagent. The preparation method comprises the following steps: preparing a buffer solution with the pH value of 6.8-7.2 and the concentration of 10-100 mmol / L; the buffer solution is a 3-(N-morpholine) propanesulfonic acid buffer solution, a phosphate buffer solution, a citric acid-dipotassium phosphate buffer solution or a citric acid-disodium hydrogen phosphate buffer solution; preparing a second solution for reducing the biological entropy; the second solution is a hydroxypropyl-beta-cyclodextrin solution, a beta-cyclodextrin solution, an agarose solution, a carboxylated chitosan solution, a sericin solution or a chitosan oligosaccharide solution; preparing a third solution required for combining the hydrogen donor and the color source, wherein the third solution is a POD-hydrogen donor solution; wherein the hydrogen donor is TOPS, TOOS, DHBS, ADOS, ADPS, ALPS, DAOS, HDAOS or MADB, and the hydrogen donor is a hydrogen donor. Preparing a fourth solution required for combining the hydrogen donor and the color source, wherein the fourth solution is a color source solution; and preparing H2O2 solid-phase liquid for preparing the vaginal secretion hydrogen peroxide hypersensitivity detection reagent based on the second solution, the third solution and the fourth solution. The invention also discloses a kit as well as a preparation method and application thereof.

The invention discloses a vagina micro-ecology automatic detection system and method based on immunofluorescence microscopic scanning and AI analysis. The system comprises a fluorescence imaging module, a motion control module, an image processing module and an AI intelligent analysis module. The motion control module is used for bearing a vaginal secretion slide sample and controlling the vaginal secretion slide sample to move in a three-dimensional space, and is matched with the fluorescence imaging module to carry out full-automatic scanning; the image processing module is used for enhancing and de-noising the collected original fluorescence image so as to improve the image quality; the AI analysis module performs multi-target recognition and quantitative analysis on the processed image based on a deep learning algorithm, and counts the number and proportion of various bacteria and cells; and finally, automatically generating a micro-ecology assessment report according to clinical rules. The full-flow automatic and intelligent detection of the vaginal secretion sample is realized, the problems of strong subjectivity and low efficiency of manual microscopic examination are effectively solved, and higher sensitivity, specificity and repeatability in vaginitis diagnosis are realized.

The invention tries to find out the coital timeline of a person via the gene mutations arising out of the parasites with demonstrated presence in semen and vaginal fluid. IT also tries to find the sexual timeline of a person by finding out the age of the parasites or mutations due to interactions with other cohabitants or in the other body, which possibly gets transferred with the semen during intercourse. This shall serve as an important tool for providing justice to rape victims. The mutations occurring due to interaction within the seminal fluid or in the body of the female are recorded and analyzed.

The application discloses a kind of based on attention mechanism and multi-scale fusion's vaginitis pathogen cell detection method and system.The method will be obtained in advance vaginal secretion immunofluorescence microscopic image input training completed optimal pathogen detection model, output the detection result of recognition trichomonad, leukocyte, epithelial cell, clue cell and spore.The application is based on deep learning algorithm to realize the automatic high-efficiency accurate detection of vaginitis pathogen cell, improve the confirmed diagnosis rate of vaginitis, greatly reduce the probability of missed detection, misdiagnosis phenomenon in diagnosis process.According to pathogen cell detection result, simulate clinical diagnosis output, realize pathogen cell classification judgment, provide reliable basis for the automation auxiliary diagnosis of vaginitis.