19 results about "Streptococcus mastitidis" patented technology

The application discloses a bocachito Nat10 antibacterial peptide, a coding sequence and use and a preparation method thereof, and relates to a method based on multiomics, a biological big data based on multiomics data of bocachito, screening and identifying an antibacterial peptide precursor gene sequence, and obtaining an amino acid sequence of the bocachito Nat10 antibacterial peptide through translation design, as shown in SEQ ID NO: 3. Through activity simulation prediction and in-vitro experiment verification, the bocachito Nat10 antibacterial peptide shows inhibitory effects on the growth activities of Streptococcus agalactiae, Salmonella enteritidis, Staphylococcus aureus, Vibrio anguillarum and Escherichia coli, especially on Staphylococcus aureus, and shows the best antibacterial effect at a concentration of 2.5 micromoles per liter. The bocachito Nat10 antibacterial peptide can be used for preparing various bactericidal preparations, including bacteriostatic agents, medicines or additives, and has good practical value and social ecological benefits.

The application discloses a recombinant strain and a preparation method thereof and application of the recombinant strain in preparation of a vaccine for Streptococcus agalactiae of tilapia, and the recombinant strain is successfully constructed by the following steps: designing an ORF4 primer, amplifying an ORF4 gene fragment from a DNA template of Streptococcus agalactiae, editing the ORF4 gene fragment into Escherichia coli by means of a CRISPR / Cas9 gene editing technology, and connecting a green fluorescent protein (GFP). The recombinant strain is subjected to immunoprotection research on the Streptococcus agalactiae of tilapia, and it is shown that the recombinant strain has a good protection effect, and can provide a solid theoretical basis and technical support for application and development of the vaccine for Streptococcus agalactiae of tilapia in the field of aquaculture.

The present application relates to the field of biotechnology, and more particularly to a SNP marker related to the level of anti-streptococcus agalactiae IgM antibody of tilapia and application thereof. The present application screens out QTLs, functional genes and key signal pathways significantly related to the level of anti-streptococcus agalactiae IgM antibody of tilapia by RNA-Seq and BSA-Seq technologies, and further identifies two SNP markers closely related to the level of anti-streptococcus agalactiae IgM antibody of tilapia, thereby providing important genetic targets and tools for molecular marker-assisted breeding of anti-streptococcus agalactiae of tilapia.

The invention belongs to the field of microorganisms, and discloses a bacterial strain having an antagonistic effect on pathogenic bacteria of cow mastitis and application of the bacterial strain, the bacterial strain SUD-8 having the antagonistic effect is paenibacillus polymyxa, and the bacterial strain SUD-8 has the antagonistic effect on the pathogenic bacteria of cow mastitis and application of the bacterial strain SUD-8 to the pathogenic bacteria of cow mastitis. The strain has an antagonistic effect on klebsiella pneumoniae, escherichia coli, staphylococcus aureus, streptococcus uberis and streptococcus dysgalactiae, and the growth speed in the cow dung back-mattress material is high.

The present application relates to the field of microbial technology, in particular to bacillus subtilis BS-1 and its application in water buffalo silage feed, the strain BS-1 of the present application has good bacteriostatic activity on escherichia coli, salmonella and streptococcus uberis, after mixing and fermenting according to a specific proportion with orange peel and cassava residue, the bacteriostatic spectrum of the obtained silage feed is significantly enhanced, which can simultaneously inhibit escherichia coli, campylobacter jejuni, salmonella, streptococcus uberis, staphylococcus aureus and streptococcus agalactiae that cause diarrhea and mastitis. In the feed, after mixing 60%-75% of orange peel and 25%-40% of cassava residue, the crude protein content of the fermentation product is significantly improved, and the fiber content is reduced. Feeding test shows that the feed can significantly improve the milk yield and milk quality of lactating water buffalo, and effectively alleviate diarrhea. The present application provides a safe, efficient and nutritious silage feed with disease prevention function.

The application belongs to the technical field of Chinese herbal medicines, and particularly relates to a Chinese herbal medicine composition for resisting Streptococcus agalactiae, a preparation method and application thereof. Raw materials for preparing the Chinese herbal medicine composition include cortex mali and cumin. The Chinese herbal medicine composition is easy to obtain and simple to prepare, has no chemical residues, is not prone to drug resistance, has significant bacteriostatic activity on Streptococcus agalactiae, and can be applied to the prevention and treatment of Streptococcus agalactiae disease of fish such as tilapia.

The invention discloses a condom added with sophocarpidine hyaluronic acid lubricating liquid and a preparation method of the condom, and belongs to the technical field of condom production. The core of the method is that after matrine is dissolved, the matrine is added into hyaluronic acid lubricating liquid for the condoms, the hyaluronic acid lubricating liquid containing the matrine is prepared through stirring and mixing, the concentration of the matrine in the hyaluronic acid lubricating liquid is 7.7-25.0 mg / g, when the condoms are subjected to inner packaging, the matrine-containing lubricating agent is added into the condoms, and when the condoms are subjected to inner packaging, the matrine-containing lubricating agent is added into the condoms. And preparing the condom added with the sophocarpidine-containing hyaluronic acid lubricating liquid. According to the condom, inflammation generated after vaginal epithelial cells are stimulated by bacterial structural components is improved through matrine, and the common pathogenic bacteria such as staphylococcus aureus, escherichia coli, enterococcus faecalis and streptococcus agalactiae of vaginitis are inhibited in the using process; and the up-regulation level of NF-kB phosphorylation generated after lipopolysaccharide and peptidoglycan stimulate vaginal epithelial cells is relieved.

The invention relates to immunogenic compositions comprising a capsular polysaccharide (CP) from Streptococcus agalactiae, commonly referred to as group B streptococcus (GBS), conjugated to a carrier protein, and optionally including an aluminum-based adjuvant. The invention further relates to methods for inducing an immune response in subjects against GBS and / or for reducing or preventing invasive GBS disease in subjects using the compositions disclosed herein. The resulting antibodies can be used to treat or prevent GBS infection via passive immunotherapy.

The application discloses a tilapia Npy antibacterial peptide, a coding sequence, and a use and a preparation method thereof, and relates to the following technical scheme: based on a multi-omics method, an antibacterial peptide precursor gene sequence is screened and recognized from a tilapia multi-omics data set based on biological big data, and then the tilapia Npy antibacterial peptide amino acid sequence is obtained through translation design, as shown in SEQ ID NO: 3; through activity simulation prediction and in-vitro experiment verification, the tilapia Npy antibacterial peptide shows inhibitory activity to gram-positive streptococcus agalactiae, staphylococcus aureus and gram-negative escherichia coli, especially to streptococcus agalactiae and staphylococcus aureus, and shows good antibacterial effect at a low concentration (2.5 μmol / L), and can be used for preparing antibacterial products, including preparation of an antibacterial agent, preparation of a medicine or preparation of an additive, and has good practical value and social ecological benefits.

The present application relates to oligosaccharide composition and its use. The present application particularly relates to the use of oligosaccharide composition in the preparation of products for preventing and / or treating aquaculture diseases caused by Vibrio parahaemolyticus, Vibrio harveyi and Streptococcus agalactiae, wherein the oligosaccharide composition is obtained by degrading polygalacturonan or pectin by a polygalacturonase as shown in SEQ ID No: 1, under the conditions of a polygalacturonase concentration of 0.045 mg / mL to 0.055 mg / mL, a polygalacturonan concentration of 7% to 9%, and a degradation time of 12 hours to 20 hours. The present application also relates to the use of oligosaccharide composition in aquaculture. The oligosaccharide composition of the present application has good bacteriostatic effect and disease prevention and control effect in aquaculture.

A method for rapidly identifying highly virulent strains of Streptococcus agalactiae, comprising: using a laser confocal Raman spectrometer to collect single-cell Raman spectra of clinical strains, and establishing a database; classifying data of III / ST17-type highly virulent GBS strains into a first group, named as GBS-HIGH, data of non-III / ST17-type GBS strains into a second group, named as GBS-OTHERS, and data of Escherichia coli into a third group, named as ECO; randomly dividing the three groups of data into a training set, a validation set and a test set in a ratio of 7:1:2; and on the basis of a ResNet18 residual neural network architecture, changing 2D convolution in a network to one-dimensional (1D) convolution suitable for a 1D Raman spectrum, determining optimal parameters by means of simulation experiments, training the weight of a model in the training set, and then selecting optimal hyperparameters by means of a result of the model on the verification set, so as to determine a trained model.

The application discloses a kind of nanobody targeting streptococcus agalactiae SIP protein and preparation and application thereof, it is related to biotechnology field, and the nanobody that the present application is screened by nanobody phage library construction, phage screening technology, and is efficiently and specifically combined with SIP protein Nanobody, the nanobody of streptococcus agalactiae SIP protein includes VHH fragment, with the amino acid sequence shown in SEQ ID NO:1.The nanobody can be combined with streptococcus agalactiae SIP protein high specificity, and the antibody can be targeted to SIP protein by the antibody, and the application discloses a kind of nanobody targeting streptococcus agalactiae SIP protein can be applied to fish disease diagnosis and treatment.

The invention discloses a method for screening crRNA based on molecular docking and application of double crRNA in improving group B streptococcus detection. A specific virulence gene in a streptococcus agalactiae genome is taken as a target spot, two potential crRNA candidates with the best detection effect are designed and screened out, and a double crRNA enhanced CRISPR detection system is established and is combined with RPA (recombinase polymerase amplification) amplification to be used for rapid and high-sensitivity detection of streptococcus agalactiae. The detection kit can complete detection from a sample to a result within one hour, the quantitative detection linear range is 103-107 cfu / mL, and the detection limit can reach 101 cfu / mL.

The present application belongs to the technical field of genetic engineering, and particularly relates to a Streptococcus agalactiae Mu-20 and a construction method and application thereof. The present application constructs a fish-derived Streptococcus agalactiae strain with a capsule polysaccharide deficiency, no hyaluronidase activity, no hemolysis and no trehalose fermentation, and the strain number is Mu-20. The strain was preserved in the Guangdong Microbial Culture Collection Center on June 13, 2022, and the preservation number is GDMCC No: 62540. The strain does not carry a chloramphenicol resistance marker gene, has high biological safety, has no risk of virulence returning to strength, has high immunogenicity, can protect immunized fish against the invasion of Streptococcus agalactiae, and is easy to cultivate, simple to use and low in production cost. The strain involved in the present application can be used to prepare a live attenuated vaccine for fish streptococcosis, and has extremely high commercial application value.

The invention discloses a freeze-dried reagent ball special for streptococcus agalactiae CRISPR detection aiming at a tilapia mossambica tissue matrix and application of the freeze-dried reagent ball, and belongs to the field of molecular diagnosis technology and on-site rapid detection of aquatic diseases. The freeze-drying reagent ball is a three-layer spherical freeze-drying body comprising an inner core layer, an isolating layer and a shell layer, the inner core layer contains an RAA amplification component aiming at a streptococcus agalactiae cfb gene, the shell layer contains a CRISPR-Cas12a detection component and a special anti-inhibition buffer system, and the isolating layer is a solid freeze-drying material layer of which the dissolution rate is slower than that of the shell layer. Through the dissolution time sequence control of the three-layer structure, the automatic sequence that an outer shell layer firstly dissolves and neutralizes an inhibitor and presets a detection system and an inner core layer then dissolves and starts amplification is realized, and multi-step reaction is integrated into one-step closed tube operation. During detection, only tilapia tissue crude extract needs to be added, and a result is interpreted through fluorescence after constant-temperature incubation. The method effectively overcomes the problems of aquatic product tissue matrix inhibition, unstable operation of an existing single-tube method and on-site pollution risk, and is high in sensitivity, extremely simple to operate and stable at normal temperature.

The invention provides streptococcus equi and application thereof in streptococcus equi gene editing, aims to improve the endogenous gene editing efficiency of a system on streptococcus equi, and relates to the technical field of bioengineering. According to the method, by introducing a streptococcus agalactiae RecT recombinase system, the editing efficiency of a streptococcus equi CRISPR-Cas9 gene editing system for high-yield HA is improved, and the problem that a streptococcus equi genome editing tool is low in efficiency is solved.

This invention discloses a triplet real-time PCR detection method for Streptococcus agalactiae, Streptococcus dolphinus, and Lactococcus gasseri. It utilizes three primer sets and probes: one for Streptococcus agalactiae, one for Streptococcus dolphinus, and one for Lactococcus gasseri. By optimizing the degradation temperature, primer dosage, and probe dosage, this invention effectively reduces the mutual interference of the three target pathogens, improving the accuracy of the detection results. This detection method has significant application value in fish farming. The reagent kit of this invention has low preparation cost, a simple preparation process, and is easily scalable for industrial production, thus possessing broad market prospects.

The present invention relates to compositions and methods to detect Group B Streptococcus (GBS, Streptococcus agalactiae) and to identify the most prevalent genes responsible for clindamycin resistant GBS such as ermB, ermTR, ermT, lsaC and lsaE by a multiplex real-time PCR assay.

This invention discloses an attenuated strain of Streptococcus agalactiae, a vaccine, and their applications, belonging to the field of veterinary biological products technology. This attenuated Streptococcus agalactiae strain is produced by knocking out the virulent Streptococcus agalactiae strain HN016. marR The genes were obtained later; among them, marR The nucleotide sequence of the gene is shown in SEQ ID NO:1. This invention utilizes the knockout of the highly virulent strain HN016 of *Streptococcus agalactiae*. marR After gene modification, a significantly reduced virulence strain of Streptococcus agalactiae was obtained. When this strain was used to prepare a vaccine, it significantly improved the survival rate of aquatic animals infected with Streptococcus agalactiae. Therefore, this strain of Streptococcus agalactiae has good application prospects in the preparation of drugs for the prevention and / or treatment of Streptococcus agalactiae infection in aquatic animals.