The invention discloses a new traditional Chinese medicine composition for treating the disease of pyelonephritis and a preparation method thereof. The composition mainly comprises the following raw medicines: the root of kudzu vine, gypsum, rhizoma anemarrhenae, phragmites communis, mongolian snakegourd root, cape jasmine, rheum officinale, rehmanniae radix, radix scrophulariae, tree peony bark,honeysuckle, taraxacum officinale root, semen euryales, root-bark of Chinese wolfberry, amur corktree bark, oriental waterplantain rhizome, tuckahoe, belvedere fruit, pyrrosia lingua, cochinchnese asparagus root, plantain seed, ricepaperplant pith, Japanese climbing fern spore, ophiopogon japonicus, the fruit of Chinese magnoliavine, turtle shell, tortoise plastron, cornus officinalis, cherokee rose fruit and cuttlebone. The composition can be prepared into any one common oral preparation according to the conventional traditional Chinese medicine preparation method. The composition can obviously improve the sign and symptom indexes of the patients suffering from pyelonephritis and such symptoms as fever, increase of white blood cells, complications infection, uronephrosis, renal cyst, edema of four limbs, renal function asthenia, urine protein, hematuria and the like, and features confirmed clinical effects, obvious and quick effects. The composition basically combines the medicinal and editable medicines specified in the State Pharmacopoeia, thus having the advantages of low cost and no toxic side effects basically, etc.