The invention discloses a highland barley beta-glucan extract, a frozen sweet dough, preparation methods therefor and sweet dough bread. The frozen sweet dough comprises the following raw materials: frozen dough mixed powder, water, the highland barley beta-glucan extract, fresh yeast, lactic acid, whole milk powder, butter, ghee, liquid whole egg, salt, a frozen dough improver, sugar, honey and citric acid. According to the invention, the highland barley beta-glucan extract is applied to the frozen sweet bread, so that the problem that the healthcare function of the highland barley beta-glucan cannot be exerted in the bread is solved, the effect of the highland barley beta-glucan is fully exerted, and the unnecessary mutual repulsion effect is reduced; and the provided frozen sweet breadcan meet the needs of people of different ages for safe, delicious and healthy functional baked foods.