39 results about "Membranous nephropathy" patented technology

A method of treating a glomerular disease selected from the group consisting of mesangial proliferative glomerulonephritis, collapsing glomerulopathy, proliferative lupus nephritis, crescentic glomerulonephritis and membranous nephropathy in a subject comprises administering to the subject an effective amount of a glucosylceramide synthase inhibitor.

A method of treating a glomerular disease selected from the group consisting of mesangial proliferative glomerulonephritis, collapsing glomerulopathy, proliferative lupus nephritis, crescentic glomerulonephritis and membranous nephropathy in a subject comprises administering to the subject an effective amount of a glucosylceramide synthase inhibitor.

The invention discloses a super-paramagnetic iron oxide nanoparticle compound as well as a preparation method and application thereof. The super-paramagnetic iron oxide nanoparticle compound is prepared by the following steps: preparing super-paramagnetic iron oxide nanoparticles by taking Fe3O4 with surface modified with a polyethylene glycol derivative as a core; and then modifying an IL-1 antibody on the surfaces of the super-paramagnetic iron oxide nanoparticles, so that the super-paramagnetic iron oxide nanoparticle compound is prepared. The super-paramagnetic iron oxide nanoparticle compound prepared by the invention can achieve specific affinity with high-expression IL-1 in mesangial area and glomerular area of chronic glomerulonephritis in vivo and can display and quantify the early lesions of the chronic glomerulonephritis in the forms of magnetic resonance images and data; therefore, the super-paramagnetic iron oxide nanoparticle compound can be used for the early diagnosis of diseases and for the dynamic monitoring of changes of nephropathy, and a magnetic resonance molecular imaging means for noninvasive and in vivo evaluation of membranous nephropathy is provided.

This invention provides organic biomolecule markers (e.g., proteins) useful for differentiating minimal change nephrotic syndrome (MCNS) from focal segmental glomerulosclerosis (FCS), membranous nephrothropy (MN), and membranoproliferative glomerulonephritis (MPGN). This invention also provides organic biomolecule markers useful for evaluating the therapeutic value of agents for treating kidney disease.

The invention provides a heterocyclic compound containing piperidine as shown in a formula (I). The compounds are used for treating diseases and diseases related to complement alternative pathway activation, such as paroxysmal nocturnal hemoglobinuria (PNH), IgA nephropathy (IgAN) including C3 glomerulopathy (C3G), membranous nephropathy (MN) and other glomerular diseases, atypical hemolytic uremic syndrome (aHUS), age-related macular degeneration (AMD), diabetic retinopathy (DR), and other diseases associated with abnormal activation of the alternative complement pathway by inhibiting / regulating a complement factor B.

The invention provides a method for detecting a biological sample. The method comprises the following steps: (1) separating the biological sample collected from a subject to obtain a sample to be detected; and (2) detecting one or more of IgG4, PLA2R, Nephrin and NPHS2 in the sample to be detected, and adopting the obtained detection result as a preliminary basis for judging that whether the subject needs renal biopsy or not. The detection method can be used to perform primary screening on membranous nephropathy, and provides reference data for whether renal biopsy puncture in the later stageis performed or not; and the detection method is noninvasive, and has the advantages of simplicity in operation and low detection cost.

The invention discloses a traditional Chinese medicine mixture for treating the pediatric membranous nephropathy. The traditional Chinese medicine holds that the pediatric membranous nephropathy belongs to the 'edema' range and relates to the lung, spleen and kidney three-viscera malfunction. Most of the pediatric membranous nephropathy is mainly characterized by insufficiency of both the spleen and the kidney, blood stasis obstructing the collaterals and internal stagnation of fluid-dampness. The operations of treating the root and strengthening the body resistance serve as the principal, and the operations of warming kidney and eliminating damp are assisted on the basis of invigorating spleen, supplementing qi and invigorating the circulation of blood. Therefore, the traditional Chinesemedicine mixture for treating the pediatric membranous nephropathy is prepared from the following traditional Chinese medicines of plantain seed, semen coicis, rust-coloured crotalaria herb with root, herba lycopi, the root of bidentate achyranthes, rhizoma sparganii, astragalus mongholicus, boschniakia rossica, herba epimedii, rhizoma polygonati and rhizoma atractylodis, wherein the traditional Chinese medicines have the efficacy on tonifying Qi, lifting yang, removing blood stasis, and inducing diuresis to alleviate edema. The obtained traditional Chinese medicine mixture is taken by patients. According to the clinical test of our hospital, the total effective rate of the traditional Chinese medicine mixture is 93.3%, and a treatment group is obviously superior to a control group on theaspects of lowering cholesterol (CHOL) and urine protein and improving albumin (ALB) and clinic efficiency.

The invention relates to an analyzing method of membranous nephropathy tri-methyl status differential expression genes. Chromatin immunoprecipitation-high through-put (Chip-seq) sequencing is used for analyzing histone H3K9 tri-methyl status change of peripheral blood mononuclear cells (PBMCs) of a membranous nephropathy patient to obtain the membranous nephropathy tri-methyl status differential expression genes. By intensive researching the differential expression genes, pathogenesis of membranous nephropathy can be further clarified, and new paths are provided for diagnosis and treatment of membranous nephropathy. The analyzing method is reasonable and feasible in design, can effectively help to build a spectrum model of differential expression genes of membranous nephropathy, and related information, as the middle result, of membranous nephropathy can be achieved.

The invention relates to a traditional Chinese medicinal composition for treating membranous nephropathy and a method for preparing the traditional Chinese medicinal composition for treating the membranous nephropathy. The traditional Chinese medicinal composition is prepared from the following raw materials in proportions by weight: 60 to 120 parts of radix astragali seu hedysari, 15 to 30 parts of radix rehmanniae recens, 15 to 30 parts of radix rehmanniae preparata, 15 to 30 parts of gorgon fruit, 15 to 30 parts of Chinese pyrola herb, 30 to 90 parts of rhizoma smilacis glabrae, 30 to 90 parts of herba hedyotidis diffusae, 15 to 30 parts of radix salviae miltiorrhizae, 0.5 to 3 parts of hirudo recens, 6 to 12 parts of herba schizonepetae and 6 to 12 parts of radix saposhnikoviae. The raw materials are extracted according to a certain technical method, and then are made, together with pharmaceutically acceptable accessories, into a traditional Chinese medicinal oral preparation. The traditional Chinese medicinal composition which is provided by the invention has the effects of tonifying spleen and kidney, supplementing qi, nourishing yin, removing dampness through diuresis and promoting blood circulation, and is used for treating various kinds of acute and chronic nephritis, and proteinuria and edema which are caused by a nephrotic syndrome.

The invention provides a method, system and device for predicting membranous nephropathy based on machine learning, and the method comprises the steps: data collection, data preprocessing, feature screening, prediction model construction, and prediction model testing and evaluation: obtaining the data information of a to-be-retrieved patient, inputting the data information of the to-be-retrieved patient into a prediction model, and carrying out the prediction of membranous nephropathy. The prediction model predicts the membranous nephropathy according to the data information of the patient to be retrieved, in a word, the prediction model for membranous nephropathy can be developed and verified by using a machine learning method, high-efficiency and high-accuracy prediction can be realized, and the method has important research significance and use value for early treatment and prevention of membranous nephropathy.

The invention discloses a traditional Chinese medicine preparation for treating membranous nephropathy. The traditional Chinese medicine preparation is prepared from angelica sinensis, prepared rehmannia root, ligusticum wallichii, parched white peony root, fructus psoraleae, teasel root, eucommia ulmoides, wolfberry fruits, herba epimedii, astragalus membranaceus, rhizoma curculiginis, rhizoma drynariae, cordyceps sinensis, fructus alpiniae oxyphyllae, cynomorium songaricum, amethyst, odobenus rosmarus, salvia miltiorrhiza, sargent gloryvine, dandelion, honeysuckle, lophatherum gracile, glossy privet fruits, mulberries and dogwood according to a certain weight ratio. The traditional Chinese medicine preparation has the efficacies of tonifying the liver and kidneys, tonifying the kidneys, invigorating yang, boosting essence and filling marrow, and is significant in treatment effect.

The present disclosure provides a method for treating and / or preventing nephrotic syndrome, such as but not limited to minimal change disease and membranous nephropathy, and conditions related to nephrotic syndrome, such as but not limited to, proteinuria and edema, as well as diabetic nephropathy, diabetes mellitus, lupus nephritis or primary glomerular disease. The present disclosure further provides methods for reducing proteinuria and other disease states as discussed herein. Such methods comprise the therapeutic delivery of an Angptl4 polypeptide or Angptl4 polypeptide derivative to a subject.

The present disclosure provides a method for treating and / or preventing a condition characterized as a nephrotic syndrome, such as but not limited to minimal change disease (MCD) and membranous nephropathy (MN), and conditions related to nephrotic syndrome, such as but not limited to, proteinuria and edema, as well as diabetic nephropathy, diabetes mellitus, lupus nephritis or primary glomerular disease. The present disclosure further provides methods for reducing proteinuria and other disease states as discussed herein. Such methods comprise the therapeutic delivery of an Angptl4 polypeptide or Angptl4 polypeptide derivative to a subject.

The invention discloses a traditional Chinese medicine preparation for treatment of membranous nephropathy. The traditional Chinese medicine preparation comprises, by weight, 10-30 parts of cortex magnoliae officinalis, 5-20 parts of cherokee rose, 10-35 parts of indian buead, 5-25 parts of large head atractylodes rhizome, 5-10 parts of flower of Japanese camellia, 5-15 parts of fortune eupatorium herb, 10-35 parts of radix astragali, 10-25 parts of dwarf lilyturf tuber, 10-20 parts of semen plantaginis, 5-25 parts of common andrographis herb, 5-25 parts of white Chinese peony root, 10-25 parts of mosla scabra, 10-20 parts of cortex eucommiae, 5-25 parts of all-grass of desertliving cistanche, 5-20 parts of stir-fried Chinese yam, 5-15 parts of nippon hawthorn fruit, 5-25 parts of danshen root, 5-15 parts of prepared rhizome of adhesive rehmannia, 5-15 parts of lalang grass rhizome, 15-20 parts of oriental waterplantain rhizome and 5-20 parts of caulis perillae. The traditional Chinese medicine preparation special for treating membranous nephropathy is free of toxic and side effects and has functions of curing, caring and nourishing kidney. As components of the traditional Chinese medicine preparation are supplementary to each other, therapeutic effect is improved while curative ratio is increased.

The present disclosure provides a method for treating and / or preventing nephrotic syndrome, such as but not limited to minimal change disease and membranous nephropathy, and conditions related to nephrotic syndrome, such as but not limited to, proteinuria and edema, as well as diabetic nephropathy, diabetes mellitus, lupus nephritis or primary glomerular disease. The present disclosure further provides methods for reducing proteinuria and other disease states as discussed herein. Such methods comprise the therapeutic delivery of an Angptl4 polypeptide or Angptl4 polypeptide derivative to a subject.

The invention discloses a new application of selepravastatin, namely an application of selepravastatin in preparation of a medicine for treating nephropathy. The application has the advantages that anew medicine for treating the nephropathy is provided, the effect of treating the membranous nephropathy is achieved through the immune effect, and finally animal experiments prove that the selaprivapeptide can reduce total urinary protein, urinary albumin and systolic pressure of membranous nephropathy model animals, so that the selaprivapeptide is a new immune agent and a novel therapeutic meansfor treating the membranous nephropathy.

A traditional Chinese medicine composition for treating membranous nephropathy, belonging to the technical field of membranous nephropathy treatment, comprising 5-10 parts of raw astragalus, 0.5-1 part of whole worm, 2-4 parts of fenugreek, and 3 parts of chuanxiong in terms of parts by mass. ‑5 parts, Zhi Licorice 2‑3 parts. The usage method of the traditional Chinese medicine composition is also disclosed. The traditional Chinese medicine composition mainly strengthens the spleen and kidney, activates blood and removes dampness, and attacks toxins and dredges collaterals. To treat membranous nephropathy, combined with the use method of the traditional Chinese medicine composition, the therapeutic effect is remarkable without any side effects.

The invention provides a use of an immune composition in the preparation of a reagent for noninvasive detection of membranous nephropathy. The immune composition comprises one or more of an anti-IgG4antibody, an anti-PLA2R antibody, an anti-Nephrin antibody and an anti-NPHS2 antibody, and the noninvasive detection of the membranous nephropathy comprises detection of the one or more of IgG4, PLA2R, Nephrin and NPHS2 in a biological sample collected from a subject. The immune composition is used for preparing the reagent for the noninvasive detection of membranous nephropathy, and a detection result highly consistent with renal biopsy can be obtained through the noninvasive detection, so the reagent has high detection accuracy for the membranous nephropathy; and the detection is noninvasive. The invention provides a rapid and noninvasive detection method for the differential diagnosis of the membranous nephropathy.

The present application discloses a pathological image classification method, device, equipment and storage medium for membranous nephropathy. The method includes: acquiring a renal biopsy glomerular pathological image; inputting the renal biopsy glomerular pathological image into a pre-trained The segmentation module of the MTLSC-Net neural network model obtains the segmentation mask image of the glomerular basement membrane and its surrounding immune complexes; the segmentation mask image is input into the classification module of the MTLSC-Net neural network model to obtain the membrane Classification results of pathological images of nephropathy. According to the pathological image classification method provided by the embodiment of the present application, based on the multi-task deep learning neural network model, the features related to the classification of the pathological images of membranous nephropathy are obtained by segmentation, and the pathological images of membranous nephropathy are quickly and accurately classified, so as to assist the pathologist to classify the pathological images of membranous nephropathy. The disease stage of membranous nephropathy is classified, which effectively improves the work efficiency of pathologists.

The present disclosure provides methods for treating lupus nephritis in an individual having lupus. In some embodiments, the methods comprise administering to the individual an effective amount of a type II anti-CD20 antibody. In other aspects, the disclosure provides methods for treating membranous nephropathy.

The invention discloses application of a reagent for quantitatively detecting the phosphorylation level of a T567 site of a protein EZR in preparation of a membranous nephropathy diagnostic reagent. In the research process, the applicant finds that the phosphorylation level of the EZR phosphorylation site T567 is obviously reduced in an IMN group compared with a control group, and the phosphorylation level is possibly gradually reduced along with disease progression. Downregulation of the phosphorylation level of T567 may participate in podocyte damage in the IMN disease progression process. And the statistical results of the immunohistochemical staining intensity and the cell positive rate of the slice further prove that the phosphorylation level of the EZRT567 site is indeed reduced in the renal biopsy tissue of the IMN patient, so that the membranous nephropathy can be diagnosed through the reagent capable of quantitatively detecting the phosphorylation level of the T567 site of the protein EZR.

Provide are a diagnostically useful carrier coated with a recombinant polypeptide comprising SEQ ID NO: 1, an isolated autoantibody binding specifically to a polypeptide having SEQ ID NO: 1, a kit comprising the carrier, a method for the diagnosis of a membranous nephropathy (MN) that includes detecting the presence or absence of an autoantibody binding specifically to a polypeptide having SEQ ID NO: 1 in a liquid sample comprising antibodies from a subject, a use of an autoantibody binding specifically to a polypeptide having SEQ ID NO: 1 or a polypeptide comprising SEQ ID NO: 1, and a aqueous solution comprising an autoantibody binding specifically to a polypeptide having SEQ ID NO: 1.

The invention discloses a usage of hematoxylin for preparing a drug for treating immunity glomerulonephritis. It is discovered from research to the immunity glomerulonephritis of a membranous nephropathy animal model that the hematoxylin achieves a purpose of treating membranous nephropathy through the following three aspects: 1) the hematoxylin has functions of reducing a urine protein, improvinglipid metabolism disorder, and improving a plasma albumin level; 2) the hematoxylin has the function of improving a kidney tissue pathomorphism injury; and 3) the hematoxylin has the function of reducing a vascular endothelial growth factor (VEGF) level in blood serum, through inhibiting VEGF secretion, glomerular filtration membrane permeability is adjusted, an excretion rate of the urine protein is reduced, and a renal function is protected. The usage is capable of acknowledging that the hematoxylin has the adjusting and treating functions to the immunity glomerulonephritis, and providing anew technical measure for therapy of the immunity glomerulonephritis, and has extensive application prospect in the field of the therapy of the immunity glomerulonephritis.

The present disclosure provides a polypeptide and method for treating and / or preventing nephrotic syndrome, such as but not limited to those associated with minimal change disease and membranous nephropathy, and conditions related to nephrotic syndrome, such as but not limited to, proteinuria and edema, as well as diabetic nephropathy, diabetes mellitus, lupus nephritis or primary glomerular disease. The present disclosure further provides methods for reducing proteinuria and other disease states as discussed herein. Such methods comprise the therapeutic delivery of an Angptl4 polypeptide or Angptl4 polypeptide derivative to a subject.

The invention relates to an analyzing method of membranous nephropathy tri-methyl status differential expression genes. Chromatin immunoprecipitation-high through-put (Chip-seq) sequencing is used for analyzing histone H3K9 tri-methyl status change of peripheral blood mononuclear cells (PBMCs) of a membranous nephropathy patient to obtain the membranous nephropathy tri-methyl status differential expression genes. By intensive researching the differential expression genes, pathogenesis of membranous nephropathy can be further clarified, and new paths are provided for diagnosis and treatment of membranous nephropathy. The analyzing method is reasonable and feasible in design, can effectively help to build a spectrum model of differential expression genes of membranous nephropathy, and related information, as the middle result, of membranous nephropathy can be achieved.

The invention provides a traditional Chinese medicine composition for treating nephropathy. The traditional Chinese medicine of the present invention is mainly made of the following components: ephedra, aconite, dried ginger, fried Atractylodes macrocephala, Poria cocos, and roasted licorice. Adding an extra burden to the patient's kidneys can also produce good clinical therapeutic effects, and at the same time, the effect is quicker.