The invention provides a micro-nano
optical fiber probe for biochemical component detection based on
infrared spectroscopy and detection equipment, and a preparation method of the micro-nano
optical fiber probe comprises the following steps: S1, selecting one section of a multimode
quartz optical fiber as a sensing area, and then removing a cladding layer and a
coating layer of the sensing area to
expose a
bare fiber; s2, dripping a mixed acid solution prepared from
hydrofluoric acid and
citric acid to the
bare fiber to corrode the
bare fiber, and then cleaning and blow-
drying; immersing the treated multimode
quartz optical
fiber into a
silane coupling agent solution, reacting for 0.5-1 hour, and then
drying; s3, immersing the multimode
quartz optical
fiber treated in the step S2 into a solution containing silver nanoparticles, and reacting for 10-20 minutes to obtain an optical
fiber modified by the silver nanoparticles; and S4,
sputtering a
gold layer on the surface of the silver-modified optical fiber by adopting a vacuum magnetron
sputtering technology to obtain the micro-nano
optical fiber probe with the silver-
gold film layer. After the surface of the silver
nanostructure is covered with the
gold film,
oxygen, water, corrosive molecules and the like are effectively prevented from making direct contact with silver. In addition, both the gold and the silver can support a
surface plasmon resonance (SPR) effect, and particularly, the prepared detection equipment can ensure the sensitivity of the SPR.