The invention discloses a pretreatment
toxin detection method and device based on agricultural products, and relates to the field of trace detection.The detection method comprises the following steps that S1, an agricultural product sample is homogenized, mixed with an
ethanol-water solution containing
amylase, subjected to an enzymolysis reaction and subjected to ultrasonic treatment, and a sample extracting solution is obtained; s2, adding an inorganic salt
demulsifier, mixing, and centrifuging to obtain a sample filtrate; s3, loading the sample to a cation exchange
solid-phase extraction column, sequentially leaching and eluting, and collecting an eluent; and S4, concentrating and redissolving, reacting with a color developing agent, measuring the
absorbance at a specific
wavelength, and calculating the
toxin content. Alpha-
amylase specific enzymolysis is adopted to destroy a
starch net structure,
toxin wrapping is avoided, the extraction efficiency is improved,
starch colloid is effectively removed through
salting-out demulsification and low-temperature
centrifugation, a high-
clarity extracting solution is obtained, matrix interference is effectively removed, and high-sensitivity and high-reliability visible
light detection of trace toxins in high-
starch agricultural products is achieved.