Methods and apparatus for measuring the light absorbance of a substance in a solution

US20150153272A1Active Publication Date: 2015-06-04CYTIVA SWEDEN AB
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Patent Information

Authority / Receiving Office
US · United States
Current Assignee / Owner
CYTIVA SWEDEN AB
Publication Date
2015-06-04

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Abstract

Disclosed is an apparatus for measuring the absorbance of a substance in a solution, comprising: i) a sample cell (30) of known path length (b) for containing said solution (S), said cell being transparent to light of a predefined wavelength spectrum; ii) plural LED's each being independently operable by means of a controller (25) each for emitting light, within said predefined wavelength spectrum, along a light path; iii) a band pass filter (22) in the light path; iv) a beam splitter (24) for dividing light from said source propagating along the path into a first portion and a second portion, said first portion being directable by the beam splitter toward a reference detector (42) and said second portion being directable into the cell (30); v) a reference detector (42) for detecting the intensity of said first portion of light directed by said beam splitter; and vi) a sample detector (40) for detecting the intensity of the second portion propagating from the cell; the apparatus allowing a sample in the cell to be inexpensively subjected to more than one wavelength of light for quicker or more accurate analysis.
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Description

[0001] The present invention relates to methods and apparatus for measuring the absorbance of light of a substance in a solution using visible or non visible light, the substance having a capacity to absorb light at a specific wavelength or wavelengths. The methods and apparatus have particular utility in detecting the concentration of proteins and nucleic acids for example where such proteins or nucleic acids are refined during liquid chromatography.FIELD OF THE INVENTION

[0002] The invention relates to methods and apparatus for measuring the absorbance of a substance in a solution, typically a substance exhibiting UV light absorption at a wavelength of 400 nm or less.BACKGROUND OF THE INVENTION

[0003] Many substances absorb ultra violet or visible light due to their chemical composition. The absorption of light by substances has been used as the basis for detecting the presence of, and measuring the concentration of, such substances for many years. The concentration of the substance ca...

Claims

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