The invention discloses an overexpression
antigen cell fixed dry-type preservation method for cellular
immunofluorescence, particularly relates to the technical field of
cell biology, and relates to an overexpression
antigen cell fixed dry-type preservation method for cellular
immunofluorescence. The overexpression
antigen cell fixed dry-type preservation method comprises the following steps: cleaning to-be-preserved cells by using a 1 * PBS solution, fixing by using a PFA stationary liquid, cleaning again by using the 1 * PBS solution, repairing by using an antigen repairing liquid, sealing by using a sealing liquid, carrying out
preservative treatment by using a preservation
preservative liquid, and carrying out air-
drying and
cryopreservation. According to the present invention, the
ammonium sulfate-based antigen repair liquid is introduced, the cells are subjected to mild treatment after fixation, and the antigen epitopes shielded due to
paraformaldehyde excessive cross-linking are reversibly opened by using the suitable
ion strength, such that the antigen and
antibody binding capacity is significantly recovered, and the
antigen binding preservation capacity of the overexpression antigen cells is improved.