73 results about "Preservation fluid" patented technology

In one embodiment, a preservation apparatus is described that includes a storage compartment. The storage compartment is configured to hold an organ or tissue and a preservation fluid. A cover assembly is configured to engage the storage compartment. The cover assembly includes a support element, wherein the support element together with the storage compartment define a storage chamber. The cover assembly also includes a lid and a gas permeable membrane disposed between the lid and the support element. The gas permeable membrane and the support element together define a perfusion chamber configured to hold preservation fluid and an organ or tissue during use.

A system and method for retaining a tissue specimen obtained via a collection tube is provided. Embodiments provide a system including a collection device removably and serially disposed between a collection tube and a suction tube so as to allow fluid communication therebetween via the collection device. The collection device includes a screen for retaining a tissue specimen drawn into the collection device by and towards the suction tube while allowing any fluid accompanying the tissue to be drawn through the collection device and into the suction tube. The system also includes one or more end caps for sealing the collection device such that the collection device may store and / or transport the retained tissue specimen in a preservation fluid. A system for identifying and organizing collection devices retaining tissue specimens from various anatomical regions is also provided.

In one embodiment, a preservation apparatus is described that includes a storage compartment. The storage compartment is configured to hold an organ or tissue and a preservation fluid. A cover assembly is configured to engage the storage compartment. The cover assembly includes a support element, wherein the support element together with the storage compartment define a storage chamber. The cover assembly also includes a lid and a gas permeable membrane disposed between the lid and the support element. The gas permeable membrane and the support element together define a perfusion chamber configured to hold preservation fluid and an organ or tissue during use.

In one embodiment, a preservation apparatus is described that includes a storage compartment. The storage compartment is configured to hold an organ or tissue and a preservation fluid. A cover assembly is configured to engage the storage compartment. The cover assembly includes a support element, wherein the support element together with the storage compartment define a storage chamber. The cover assembly also includes a lid and a gas permeable membrane disposed between the lid and the support element. The gas permeable membrane and the support element together define a perfusion chamber configured to hold preservation fluid and an organ or tissue during use.

The invention relates to the technical field of organ transplantation, and particularly discloses perfusion preservation fluid for an isolated liver. The perfusion preservation fluid for the isolated liver comprises donor whole blood, albumin, antibiotics, insulin, heparin, compound amino acid, glucose, dexamethasone, sodium chloride, sodium bicarbonate, normal saline, ATP-MgCl2 and S-adenosyl methionione which are mixed according to proportion. The isolated liver is preserved in the perfusion preservation fluid for 50 hours, the damage to liver cells is less, no complication occurs, the requirements of organ transplantation can be met, the survival time of a graft can be prolonged, and the survival rate of the graft in a host is improved; the perfusion preservation fluid is low in production cost and has the practical application value.

The invention discloses a fresh amnion preservation fluid, a fresh amnion preservation method and application thereof. The fresh amnion preservation fluid is made by adding water for injection to 9.0-12.0g of DMEM culture medium, 10.0-25.0g of chondroitin sulfate, 0.5-1.0g of sodium hyaluronate, 4.0-5.0g of HEPES, 10.0-15.0g of dextran, 1.0-6.0ml of gentamycin, 24.0-30.0mg of dexamethasone, 10.0-15.0ml of non-essential amino acid, 0.50-0.95g of glutathione, 0.0-50.0ml of fetal calf serum to 1000ml. The fresh amnion preservation method comprises the following steps: separating the amnion by a blunt, rinsing the amnion clean, and then processing the amnion by an antibiotic; cutting and packaging the amnion into a container with the amnion preservation fluid; storing the amnion preservation fluid in a refrigerator with the temperature of 4 DEG C, and rinsing hands clean with aseptic phosphate buffer before use. The fresh amnion preservation fluid has the following advantages: 1. the amnion preservation fluid is only stored in the refrigerator with the temperature of 4 DEG C; 2. the shelf life is prolonged to at least 20-30 days; 3. the amnion preservation fluid can be in batch processing and centralized storage, and 4. the amnion preservation fluid can be applied to various amnion transplantation repairs, for example (1) ocular surface reconstruction as a conjunctival substitute, (2) amnion transplantation for treating symblepharon, and (3) treatment of corneal ulcer caused by various reasons and the like.

A head unit is inverted so that an outflow port is in the lowest position. Because the top wall of the buffer tank slopes downwards towards the outflow port, all remaining cleaning fluid that was introduced during manufacture is removed. Also, a notch is provided in the bottom end of an ink introduction port. The notch faces towards the outflow port so that bubbles in the ink exit from the notch and are guided towards the outflow port. The head unit is shipped while the buffer tank is filled with preservation fluid that has high wettability and any openings to the outside air are closed off with covers. When the head unit is used, the preservation fluid is sucked by a purging device and then ink, which has a high affinity for the preservation fluid, is smoothly introduced. Also, the inner surface of the filters are treated to increase the wettability by ink after the filters are fixed to the bottom lid. Afterwards the bottom lid is fixed to the top lid to form the buffer tank.

The invention provides a blood RNA preservation tube. The blood RNA preservation tube comprises a tube body, a tube cap and an RNA preservation fluid, wherein the blood RNA preservation tube has negative pressure, the volume ratio of the negative pressure to the RNA preservation fluid is (1:1)-(10:1), and the RNA preservation fluid comprises a denaturant. A method for preserving blood by use of the blood RNA preservation tube comprises steps as follows: (1) blood collection with an anticoagulation blood collection tube; (2) blood transfer: the anticoagulation blood collection tube is communicated with the blood RNA preservation tube, and the negative pressure in the blood RNA preservation tube forces blood to enter the blood RNA preservation tube; (3) pretreatment: the blood RNA preservation tube is centrifuged at a high speed for 1 min, then left to stand at the room temperature for 10 min and preserved at the required temperature. Degradation of RNA in blood preserved with the bloodRNA preservation tube can be remarkably inhibited, so that the preservation stability and the extraction rate of blood RNA are increased.

A kit for the packaging and shipping of components of an explanted medical device. The kit includes sealable containers to individually package the components of the explanted medical device. The kit also includes dressing presoaked in a preservation fluid, the dressing being provided in at least one sealed container prior to the removal of the explanted medical device to maintain the dressing a presoaked, sterile condition. The amount of preservation fluid applied to the dressing is controlled, thereby eliminating the need for a reservoir of fluid to be provided.

The invention discloses specimen preservation fluid good in preservation effect and a preparation method of the specimen preservation fluid. The preservation fluid is mainly prepared from 50% glutaraldehyde, polyoxyethylene aliphatic alcohol ether, industrial alcohol, benzalkonium bromide, phenol, glycerinum, ethylenediamine tetraacetic acid disodium salt and water. The prepared preservation fluid is free of formaldehyde, slow in volatilization, free of pungent gas, quite low in toxicity, high in safety, good in preservation effect and capable of making a specimen good in color and luster, preventing the specimen from mildew after long-term preservation and making the specimen soft in texture.

A multipurpose SCF device comprises: a raw material feeding assembly which comprises a raw material storage tank and a feeding pump; an SCF control assembly which comprises a carbon dioxide storage tank, a normal pressure pump, a booster pump and first to fifth SCF connecting assemblies, wherein the carbon dioxide storage tank, the normal pressure pump and the booster pump are sequentially connected in series; a heat preservation control assembly which comprises a heat preservation fluid storage tank, a pump, and first to fifth heat preservation connecting assemblies, wherein the heat preservation fluid storage tank and the pump are sequentially connected in series; an SCF tank body assembly which comprises at least five SCF tank bodies, wherein each SCF tank body comprises an upper cover,a lower cover, a tank main body and a heat preservation jacket; and a product collecting assembly which comprises one or more product storage tanks used for collecting products from the first to fifth SCF tank bodies, wherein the upper cover and the upper part of each SCF tank body of the SCF tank body assembly are combined in a quick release manner, and the lower cover and the lower part of eachSCF tank body of the SCF tank body assembly are combined in a quick release manner.

A method and kit for preserving a fuel system of an aircraft engine outside of an engine test cell in preparation for a period of inactivity of the aircraft engine are disclosed. The method comprises: supplying preservation fluid to the fuel system of the aircraft engine; generating a signal to cause the opening of a valve of the fuel system using a control device other than the electronic engine controller of the aircraft engine; and driving a fuel pump of the fuel system to cause some of the preservation fluid to flow through the open valve and in at least part of the fuel system of the aircraft engine.

The invention relates to an erythrocyte preservation fluid. The erythrocyte preservation fluid comprises the following components: 0.2-0.6mg / ml adenine, 20-50mg / ml glucose, 5-15mg / ml sodium citrate, 0.02-0.06mg / ml sodium dihydrogen phosphate, 1.0-3.0mg / ml anticoagulation agents, and 5-20mg / ml film protective agents, wherein the anticoagulation agents are selected from one or more of EDTA-3K and EDDHA salts. By the aid of the erythrocyte preservation fluid, the coagulability of erythrocytes in the preservation process can be reduced, erythrocyte preservation time can be prolonged, and erythrocyte hemolysis can be reduced.

The invention provides aqueous solution for preserving tissues and organs. The aqueous solution is extracellular, purified polyethylene glycol 35000 in molecular weight. Compared with preservation fluid currently on sale, the aqueous solution improves biochemical parameter and characteristics of functional parameters of perfused organs.

The invention discloses automatic tissue specimen sampling equipment which comprises a bottom plate. A collecting mechanism, a liquid filling mechanism, a capping mechanism and a conveying mechanism are sequentially arranged on the bottom plate; the collecting mechanism is used for collecting tissue specimens on biopsy forceps into specimen bottles; the liquid filling mechanism is used for automatically filling quantitative preservative liquid into the specimen bottles; the capping mechanism is used for automatically capping bottle caps at the open ends of the specimen bottles; and the conveying mechanism is used for sequentially conveying the specimen bottles to corresponding stations of the collecting mechanism, the liquid filling mechanism and the capping mechanism, so that automatic collection, liquid filling and packaging of the tissue specimens are realized. The automatic tissue specimen sampling equipment disclosed by the invention is simple in structure, the processes of separation, collection, filling, capping and the like of the tissue specimens are integrated and automatically completed, and manual operation is not needed in the period, so that the automation degree is high, and the packaging efficiency is high.

The invention discloses a C-reactive protein preservation fluid, a calibration product, a quality control product and a detection kit. The C-reactive protein preservation fluid comprises a buffer solution, 0.9-9.0 wt% of sodium chloride, 10-30 wt% of sorbitol, 0.5-5 wt% of sucrose, 0.05-0.2 wt% of a surfactant, 0.05-0.1 wt% of a preservative and 0.5-5 wt% of a protein protective agent. The C-reactive protein preserving fluid can prolong the shelf life of low-temperature preservation of C-reactive protein, the effect of normal-temperature preservation can be achieved through mutual cooperationof all the components, waste caused by improper low-temperature preservation can be avoided by using the C-reactive protein preserving fluid, and the detection cost is reduced.

The invention discloses a placenta preservation method, placenta preservation fluid and a placenta preservation fluid preparing method. The placenta preservation fluid contains cord plasma and / or glucose, penicillin-streptomycin and low molecular dextran-40. The placenta preservation fluid preparing method comprises the step of adding 3-5 v / v% of cord plasma, 5 m / v% of glucose solution, 1X-3X of penicillin-streptomycin and low molecular dextran glucose injection to PBS, wherein the final concentration of low molecular dextran-40 is 1 m / v%. According to the placenta preservation method, placentas are soaked in the placenta preservation fluid to be preserved in a dark place at 2-4 DEG C. An ion buffer system and a nutrition system of the preservation fluid are improved, constituents and content of constituents in the preparation are optimized, long-time preservation of in vitro placentas can be achieved, the vigor of placental mesenchymal stem cells can be well maintained, and functional variation of cells is avoided.

The invention provides a special normal-temperature preservation fluid for a dog mesenchymal stem cell, which is prepared from dog blood albumin, normal saline, glucose, citric acid, and sodium chloride. The invention further provides an application method of the special normal-temperature preservation fluid for the dog mesenchymal stem cell. The method includes steps of adding the a dog mesenchymal stem cell performed with transportation pre-treatment in the special normal-temperature preservation fluid for the dog mesenchymal stem cell; adjusting cell density to 5x105-6 / ml; taking 1 ml cellsuspending fluid in 2 ml EP pipe and sealing. The special normal-temperature preservation fluid for the dog mesenchymal stem cell is easy to prepare and low in preparation cost; the fluid has the effect of storing the dog mesenchymal stem cell under the constant temperature, and can effectively prevent the dog mesenchymal stem cell from death or clustering.

The invention discloses an umbilical cord preservation fluid and an umbilical cord preservation method. The umbilical cord preservation fluid is prepared from 15-20 mmol of hyperbranched polyglycerol,99-101 mmol of lacturonic acid, 24-26 mmol of monopotassium phosphate, 4-6 mmol of magnesium sulfate, 4-6 mmol of adenosine, 2-4 mmol of glutathione reduction type, 6-10 mg of dexamethasone, 0.5-1.5mmol of allopurinol, 50-70 mmol of sucrose, 40-60 g of dextran, 18-22 mg of verapamil, 4-6 ml of sodium hydroxide and 19-21 ml of potassium hydroxide. When an umbilical cord is preserved , the cleanedumbilical cord is cut into small sections with the length of 3-5 cm, then immersed into a preservation liquid and preserved in an environment with the temperature of 3-5 DEG C. By adopting the preservation liquid, the problem that the umbilical cord cannot be preserved for a long time after being isolated can be effectively solved.

A system and method for retaining a tissue specimen obtained via a collection tube is provided. Embodiments provide a system including a collection device removably and serially disposed between a collection tube and a suction tube so as to allow fluid communication therebetween via the collection device. The collection device includes a screen for retaining a tissue specimen drawn into the collection device by and towards the suction tube while allowing any fluid accompanying the tissue to be drawn through the collection device and into the suction tube. The system also includes one or more end caps for sealing the collection device such that the collection device may store and / or transport the retained tissue specimen in a preservation fluid. A system for identifying and organizing collection devices retaining tissue specimens from various anatomical regions is also provided.

The invention discloses a chocolate syrup pump which consists of a gear pump, a cycloid reducer, a motor, and a frame. The gear pump consists of a pump cover, a pump body and a pair of meshing gears. Serving as an output shaft of the gear pump, a shaft of a driving gear in the pump body is linked to an input shaft of the cycloid gear reducer via a coupler, an output shaft of the cycloid reducer is linked to an input shaft of the motor via a coupler, and the gear pump, the cycloid reducer and the motor are all fixed to the frame. In the manner, the transmission of a heat-preservation fluid medium can be performed in an all-weather way by virtue of a gear extrusion principle and a hot-water heat preservation function of a jacket. The chocolate syrup pump has a compact, reasonable, stable and reliable structure, and a low failure rate. In fact, an inlet and an outlet on the left and right sides of the pump can be adjusted freely according to special requirements of production sites, thereby greatly facilitating installation of the pump by a user.

The invention discloses a kit and method for detecting the PD-1 and PD-L1 expression quantity through the RNA level. The kit comprises a house-keeping-geneamplification primer, a PD-l amplification primer and a PD-L1amplification primer. By using the kit or method, the PD-1 / PD-L1 expression level can be detected through the RNA level; for tissue samples which cannot be detected with a conventionalimmunohistochemistry (IHC) of cancer patients, particularlyfor tissue samples or puncture samples soaked intissue preservation fluid, as the application range of the kit and the method is wide, a small quantity of samples are needed, FFPE samples and tissue samples of the RNA can be extracted, detection can be carried out, the kit or the method can detect the PD-1 / PD-L1 expression level of the samples.

An improved non-formaldehyde-based preservative fluid is provided, comprising deionized water, a food-grade preservative, selected from the group consisting of sodium erythorbate and stereoisomers of ascorbic acid; and a humectant, selected from the group consisting of glycerin, glycerol, propylene glycol, glyceryl triacetate, and similar hygroscopic materials. Optional lanolin, dyes, or fragrances may be added as desired. Use of the improved fluid results in a more life-like appearance of the body, better tissue preservation, low odor, and a safer and environmentally sound alternative to conventional preservation fluids.

The invention relates to a dispensing unit for dispensing fluid into an external container. The dispensing unit comprises: a connector, a reagent cylinder for storing the fluid, and a locking ring. The connector comprises an upper fastener, and a bottom wall with at least one passage therethrough. The reagent cylinder comprises at least one compartment for storing the fluid, which compartment comprises at least one opening through a cylinder bottom. The at least one opening is located so that for at least one mutual orientation of the reagent cylinder and the connector, fluid can flow from the compartment to the external container via the at least one opening and the at least one passage. The locking ring comprises a second fastener which fastens to the upper fastener of the connector, so that when the locking ring is tightened, the reagent cylinder is pressed towards the interior bottom of the connector.

The invention discloses a transport preservation fluid for umbilical cord mesenchymal stem cells. The transport preservation fluid is prepared from the following raw materials: a sodium chloride injection, alpha-mangostin, hesperidin, parthenolide, glucose, adenosine and sodium dihydrogen phosphate. The transport preservation fluid for umbilical cord mesenchymal stem cells takes the sodium chloride injection as a main component, does not contain blood products such as serum, human serum albumin and the like, and has clear and safe components. Three components of alpha-mangostin, hesperidin and parthenolide are also added as protective agents, so that the cell membrane is protected, the situation that the cell internal space structure is disordered due to the fact that the local electrolyte concentration of the cell is increased after the cell is cooled is avoided, the cell is enabled to quickly adapt to a low-temperature environment, and cell inactivation is avoided. The invention further provides a transport preservation method of the umbilical cord mesenchymal stem cells, by adopting the transport preservation fluid, short-term preservation of the cells at 4-8 DEG C is achieved, the survival rate of the cells after being preserved for 3 days is 90% or above, and clinical use requirements are fully met.

The invention discloses freeze preservation and thawing methods for three-dimensional retina tissue. The freeze preservation method includes the main steps that the three-dimensional retina tissue suitable for freeze preservation is selected, suspended in an RDM culture solution and then transferred into a freeze preservation tube, 2*freeze preservation fluid is added according to an isovolumetricratio, part of supernate is sucked away after the three-dimensional retina tissue sinks to the bottom of the tube, the freeze preservation tube is sealed and put in the environment at the temperatureof minus 80 DEG C and stays overnight to be cooled, and then the freeze preservation tube is transferred into liquid nitrogen to be stored. Finally, the stored retina tissue is thawed and then can beput into a culture box to be subjected to suspending culturing. The methods are good in repeatability and high in success rate, the survival rate of thawing is up to 80-90%, the growing process of the three-dimensional retina tissue is controllable and reliable in storage and transportation, and it is guaranteed that the thawed tissue is complete in structure and good in activity.

The invention belongs to the field of biomedicine and discloses a mesenchymal stem cell preservation solution and its preparation method and application. The mesenchymal stem cell preservation solution contains human AB type umbilical cord blood plasma and 70- 80AXaIU / ml calcium low molecular weight heparin; the human AB type umbilical cord blood plasma and mesenchymal stem cells come from the same provider. The mesenchymal stem cell preservation solution of the present invention can prolong the activity maintenance time of mesenchymal stem cells (maintain more than 90% of the activity for 72 hours at 4-15°C), and the mesenchymal stem cells are stored in the preservation solution of the present invention for 72 hours. , the cell morphology is normal, does not affect its proliferation and expansion ability, and does not affect the phenotypic characteristics of mesenchymal stem cells. The mesenchymal stem cell preservation solution of the present invention is safe and reliable in clinical application, and the AB-type umbilical cord blood plasma and umbilical cord mesenchymal stem cells are derived from the same placenta and belong to autologous plasma, which reduces the possibility of exogenous virus contamination.

The invention discloses a novel preservation solution for microbial nucleic acid in cerebrospinal fluid. The fluid comprises the following components of a nuclease inhibitor, a buffer solution, a protein denaturation inhibitor, trisodium citrate, a bacteriostatic agent and a stabilizer, and the pH value of the novel preservation solution for the microbial nucleic acid in the cerebrospinal fluid is7-8. According to the novel preservation solution for the microbial nucleic acid in the cerebrospinal fluid, the activity of nuclease can be rapidly and effectively inhibited, the nucleic acid in a cerebrospinal fluid sample is prevented from being degraded, the content of the nucleic acid in the cerebrospinal fluid is guaranteed, and meanwhile, the cell lysis is avoided, the detection backgroundnoise is reduced, and the accuracy of cerebrospinal fluid detection is improved; and the preservation fluid is relatively simple in components and low in cost, and meanwhile, the stability of the free nucleic acid can be maintained at room temperature, and the fluid is extremely suitable for wide clinical application.

The invention discloses a cell washing container, and a method and system for cell collecting and washing. The method includes pretreatment. A filter membrane is disposed in the cell washing containerand divides the inner cavity of the container into an upper cavity and a lower cavity. Negative pressure devices form negative pressure in the upper cavity to allow a cell suspension to be washed tobe in the upper cavity; liquid in the upper cavity is subjected to suction filtration to enter the lower cavity; normal saline is added into the upper cavity to resuspend cells; the abovementioned steps are repeated by a plurality of times until liquid meets a standard; a pre-cold cell preservation fluid is quantificationally added into the upper cavity and the cells are resuspended to complete cell washing. The system for cell collecting and washing includes the cell washing container, a normal saline container, a cell suspension container, an air filter, the first negative pressure device and the second negative pressure device. Beneficial effects are that: a manner combining negative pressure suction and the filter membrane is adopted to replace the cell suspension to finish cell enrichment and washing, the washing speed is high, and the efficiency of enrichment and washing is higher than that of traditional centrifugation and natural sedimentation manners and cell damage is lower.

The invention discloses a fluid heat-preservation granary which belongs to the field of food storage equipment and comprises a granary cover and a granary body, wherein the upper part of the granary body is connected to the granary cover; the heat-reservation granary also comprises a heat-preservation fluid; the granary body is an annular granary body which is composed through layered stacking of multiple annular structural parts; both the granary cover and the annular structural parts are shell bodies which are sealed internally and externally and composed of double-layer materials; upper and lower ends of the bottom of the granary cover and the annular structural parts are equipped with fluid exchange holes; the granary cover communicates with the granary body by the fluid exchange holes; a fluid inlet is formed in the top of the granary cover; and a fluid outlet is formed in the bottom of the granary body. The granary disclosed by the invention has the advantages that multiple construction materials can be used conveniently and quickly to splice a food storage device with a fluid thermal insulation and heat preservation functions; and the granary can be applied conveniently under conditions such as different food quantities, different climates and different geological conditions.