82 results about "Bupleurum" patented technology

The application belongs to the technical field of traditional Chinese medicines, and particularly relates to a preparation method of a traditional Chinese medicine composition for intractable chronic cough and application thereof; and particularly discloses a traditional Chinese medicine composition for treating intractable chronic cough, which comprises, by weight fraction, 10-40 parts of Poria cocos, 3-25 parts of fried licorice, 3-20 parts of Schisandra chinensis, 1-20 parts of dried ginger, 1-12 parts of Asarum, 3-20 parts of prepared pinellia, 3-20 parts of bitter apricot kernel, 5-40 parts of white mulberry bark, 3-25 parts of bupleurum, 3-25 parts of wind-protecting plant, 5-40 parts of north-sand peony and 1-5 pieces of centipede. The application is a pure traditional Chinese medicine prescription, has high safety and good efficacy, and can quickly relieve symptoms of intractable chronic cough patients, and is suitable for clinical popularization and application due to wide drug source.

The application belongs to the technical field of traditional Chinese medicines, and discloses a traditional Chinese medicine composition for treating abdominal distension and diarrhea of cold-heat complex type and a preparation method thereof. The technical scheme points of the traditional Chinese medicine composition are as follows: the traditional Chinese medicine composition is composed of the following raw medicinal materials in parts by weight: 3-6 parts of perilla, 3-10 parts of dried ginger, 3-12 parts of eucommia, 3-10 parts of scrophularia, 2-6 parts of coptis, 10-20 parts of fried coix seed, 6-10 parts of clear pinellia, 3-10 parts of dried tangerine or orange peel, 10-15 parts of poria, 6-10 parts of wheat-cooked atractylodes, 6-10 parts of vinegar-cured corydalis, 6-10 parts of vinegar-cured bupleurum, 6-10 parts of pericarpium zephyritae, 6-10 parts of chuanxiong, 6-10 parts of magnolia officinalis, 6-10 parts of fried hawthorn fruit, 3-6 parts of platycodon, and 3-10 parts of fried licorice. The traditional Chinese medicine composition and the traditional Chinese medicine water pill prepared from the traditional Chinese medicine composition have the effect of treating abdominal distension and diarrhea of cold-heat complex type.

The invention discloses a traditional Chinese medicine composition for treating sepsis as well as a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from 1-15 parts of sulfur, 3-25 parts of calamitas urinae hominis, 1-20 parts of wine-treated rhubarb, 10-30 parts of fructus cannabis, 10-50 parts of radix bupleuri, 5-30 parts of scutellaria baicalensis, 5-25 parts of folium isatidis, 5-25 parts of peach kernels, 5-30 parts of salvia miltiorrhiza, 5-30 parts of angelica sinensis, 5-30 parts of sophora flower charcoal, 5-25 parts of fructus gardeniae praeparatus and 5-60 parts of wine-treated fructus corni. According to the invention, through a combined mode of'monarch drug targeted oral administration and synergistic decoction of minister, assistant and guide drugs', the whole-course intervention of'upstream inhibition, midstream blocking and downstream regulation 'on sepsis immune disorder can be realized, meanwhile, the functions of warming yang, dispelling cold, cooling blood and detoxifying are taken into account, and the pro-inflammatory / anti-inflammatory balance is successfully recovered to a physiological state; the action mode is superior to that of a traditional single anti-inflammatory strategy and a traditional Chinese medicine application mode of whole-prescription decoction. In a standard animal model, the traditional Chinese medicine composition definitely shows the dose-dependent extremely remarkable effects of anti-inflammation, immunoregulation, multi-organ protection and overall condition improvement, the curative effect of a high-dose group of the traditional Chinese medicine composition is equivalent to that of a classic potent medicine, and the traditional Chinese medicine composition has more advantages in the aspects of recovering immune homeostasis, warming yang and strengthening the body resistance.

The invention discloses a traditional Chinese medicine composition for treating acute pharyngitis as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The technical problems to be solved are that the traditional Chinese medicine composition for treating acute pharyngitis is not suitable for long-term administration, is not suitable for sensitive people and is easy to damage spleen and stomach. The invention provides a traditional Chinese medicine composition for treating acute pharyngitis. The traditional Chinese medicine composition is prepared from the following raw materials: honeysuckle flower, fructus forsythiae, radix isatidis, herba menthae, radix bupleuri, fructus arctii, schizonepeta spike, gypsum, radix scutellariae, fructus gardeniae, radix platycodonis, radix paeoniae rubra, rhizoma phragmitis, semen armeniacae amarae, herba lophatheri, fructus aurantii, radix saposhnikoviae, medicated leaven, stiff silkworm and liquorice root. The traditional Chinese medicine composition disclosed by the invention can inhibit inflammatory reaction by inhibiting phosphorylation levels of PI3K / AKT and RAS / MAPK signal pathways AKT and MAPK, so that acute pharyngitis is improved.

The invention provides a traditional Chinese medicine composition for improving the survival rate of piglets and a preparation method and application thereof, and belongs to the technical field of feed additives. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 6 to 10 parts of fructus forsythiae, 15 to 22 parts of radix isatidis, 15 to 22 parts of radix scutellariae, 8 to 12 parts of honeysuckle flower, 5 to 8 parts of herba taraxaci, 10 to 15 parts of rhizoma atractylodis macrocephalae, 8 to 12 parts of poria cocos, 6 to 10 parts of rhizoma dioscoreae, 12 to 18 parts of radix astragali seu hedysari, 8 to 12 parts of radix bupleuri, 5 to 8 parts of liquorice root and 3 to 6 parts of semen ziziphi spinosae. Based on the traditional Chinese medicine theory of supporting healthy energy and giving consideration to both symptoms and root causes, a three-dimensional synergistic system of clearing heat, resisting bacteria, invigorating spleen, benefiting intestines, tonifying qi and resisting stress is constructed to adapt to the physiological characteristics of weaned piglets, so that the problems of low survival rate, slow growth and substandard quality in weaned piglet breeding are solved, and the survival rate of the weaned piglets is remarkably improved.

The application discloses a kind of oil-containing water body separation membrane for refining process of Bupleurum volatile oil and preparation method and application.The preparation method of the application is first mixed and dissolved according to proportion with cellulose acetate and cellulose nitrate powder, form casting membrane liquid, and the base membrane is prepared using non-solvent phase conversion method;Second, the film is deacetylated;After that, it is soaked in acyl chloride n-hexane solution for crosslinking;Finally, by in-situ hydrolysis of silane coupling agent on the membrane surface, a hydrophobic surface is prepared, and an oil-containing water body separation membrane for refining process of Bupleurum volatile oil is obtained.This method forms a hydrophobic layer on the surface of the membrane, which strengthens the rapid passage of Bupleurum volatile oil molecules and inhibits the penetration of water;Using the oil-containing water body separation membrane avoids the formation of emulsion during oil-water separation, showing high separation efficiency and stability, and widening the application of cellulose acetate membrane in traditional Chinese medicine preparation process.

The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating chronic refractory cough and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following fifteen traditional Chinese medicines in specific parts by weight: blackberry lily, roasted ephedra, asarum, bamboo juice pinellia ternate, raw liquorice, radix bupleuri, fried scutellaria baicalensis, fructus aurantii, platycodon grandiflorum, roasted perillaseed, almond, wild buckwheat, Japanese metaplexis pericarp, calyx seu fructus physalis and pericarpium trichosanthis. Aiming at the core pathogenesis of the chronic refractory cough, such as residual evil and turbidity and lung-qi obstruction, evil and turbidity are separately eliminated by a method of separately eliminating and discharging, and the lung-qi is diffused and restored. The traditional Chinese medicine composition is reasonable in component and remarkable in curative effect, clinical data shows that the traditional Chinese medicine composition can effectively improve core symptoms such as cough, expectoration and throat itching, no obvious adverse reaction is found, and safety is high.

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for relieving depression and clearing collaterals and a preparation, a pharmaceutical composition and application thereof. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 6-12 parts of radix bupleuri, 9-15 parts of radix curcumae, 6-12 parts of rhizoma acori graminei, 3-9 parts of fructus gardeniae, 3-9 parts of polygala tenuifolia, 9-15 parts of radix paeoniae rubra and 9-20 parts of cortex albiziae. The traditional Chinese medicine composition provided by the invention can be used for remarkably improving depression-like behaviors of mice with depression, reversing synaptic injury and improving the expression of synaptic associated proteins SYP and PSD-95. The anti-depression mechanism of the traditional Chinese medicine composition is that the traditional Chinese medicine composition has the effects of synergistically inhibiting activation of microglial cells and reducing the level of proinflammatory cytokines such as Tnf alpha, Il1beta and Il6, so that neuroinflammation is remarkably inhibited, and the traditional Chinese medicine composition has the advantages of novel formula, definite mechanism and definite curative effect for treating depression, particularly patients with anxiety and dysphoria symptoms.

The invention discloses a traditional Chinese medicine composition for chronic obstructive pulmonary disease and a preparation method thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by mass: main medicines: 15-30 parts of radix rehmanniae preparata and 3-9 parts of semen allii tuberosi; auxiliary medicines: 10-30 parts of ginseng, 15-30 parts of astragalus membranaceus, 6-12 parts of ganoderma sinense, 6-12 parts of bighead atractylodes rhizome and 3-6 parts of schisandra chinensis; adjuvant drugs: 6-12 parts of radix ophiopogonis, 6-10 parts of rhizoma anemarrhenae, 6-10 parts of radix cyathulae and 6-10 parts of platycodon grandiflorum; and conductant drugs: 3-6 parts of radix bupleuri and 3-6 parts of rhizoma cimicifugae. The composition directly refers to the core pathogenesis of lung-kidney deficiency, the adjuvants reinforce lung and kidney tonifying, spleen strengthening and superficies consolidating, the adjuvants have the effects of nourishing yin, moistening dryness, reducing phlegm and activating blood, and the medicines prevent tonifying and stagnation, are orderly in lifting and descending and promote synergistic efficacy.

The traditional Chinese medicine comprises (1) radix puerariae, (2) radix angelicae sinensis, (3) radix salviae miltiorrhizae, (4) radix codonopsis, (5) radix astragali, (6) fructus perillae, (7) fructus ziziphi jujubae, (8) radix bupleuri, (9) radix scutellariae, (10) flos carthami, (11) radix curcumae, (12) radix et rhizoma rhei, (13) pericarpium zanthoxyli, (14) radix glycyrrhizae, (15) radix ophiopogonis, (16) fructus schizandrae, (17) radix aconiti carmichaeli, (18) radix ginseng, (19) poria cocos, (20) gypsum, (21) oyster, (22) cassia twig and (23) semen plantaginis. Or extracts of the above components, the amount of the extracts being equivalent to the amount of the raw materials of the corresponding components. The invention aims at the clinical curative effect of amyotrophic lateral sclerosis diseases with different pathological manifestations. The medicine can be combined with western medicines for use.

The present application belongs to the technical field of traditional Chinese medicine, and relates to a traditional Chinese medicine composition for treating subacute thyroiditis, which comprises the following traditional Chinese medicine raw materials in a mass ratio: Bupleurum 9-12 parts; Red Peony Root 10-15 parts; Indigowood Leaf 10-15 parts; Scrophularia 9-12 parts; Glechoma 10-15 parts; Limnophila 15-30 parts; Millettia 3-6 parts; Omphalopsis 6-9 parts; and Aquilaria 10-15 parts. The present application also provides a traditional Chinese medicine preparation for treating subacute thyroiditis: the above traditional Chinese medicine raw materials are taken according to the ratio, and a pharmaceutically acceptable carrier is added to form a traditional Chinese medicine preparation, wherein the traditional Chinese medicine preparation comprises an ointment and an external use patch. The traditional Chinese medicine composition and the traditional Chinese medicine preparation for treating subacute thyroiditis have the advantages of being simple and easy to use, rapid in effect, and green and safe.

The invention discloses a compound traditional Chinese medicinal material feed additive for stress conditioning of calves as well as preparation and application of the compound traditional Chinese medicinal material feed additive. Comprising the following raw materials: 1-3 parts of schizonepeta, 1-3 parts of cassia twig, 3-9 parts of ephedra, 2-6 parts of coptis chinensis, 1-3 parts of scutellaria baicalensis, 3-9 parts of cape jasmine, 1-3 parts of poria cocos, 5-15 parts of astragalus membranaceus, 1-3 parts of bighead atractylodes rhizome, 5-15 parts of platycodon grandiflorum, 5-15 parts of radix stemonae, 3-9 parts of white mulberry root-bark, 3-9 parts of hawthorn, 3-9 parts of massa medicata fermentata fujianensis, 5-15 parts of malt, 4-12 parts of radix bupleuri, 3-9 parts of mangnolia officinalis, 2-6 parts of semen lepidii, 1-3 parts of fructus forsythiae, 3-9 parts of ligusticum wallichii, 1-3 parts of houttuynia cordata Through the synergistic effect of multiple traditional Chinese medicines, the stress of the calves can be relieved from multiple dimensions of resisting viruses, regulating intestinal flora, enhancing immunity and the like.

The application belongs to the technical field of veterinary drugs, and specifically discloses a traditional Chinese medicine composition for preventing and treating diarrhea of calves, a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials in parts by weight: Bupleurum 5-10 parts, Paeonia 1-5 parts, Fructus Aurantii 1-5 parts, Alisma 1-5 parts, Atractylodes 5-10 parts, Astragalus 5-10 parts, Scutellaria 1-5 parts, Saposhnikovia 1-5 parts, Poria 1-5 parts, Pericarpium Citri Reticulatae 1-5 parts, Gardenia 1-5 parts, and Glycyrrhiza 1-5 parts. The traditional Chinese medicine composition provided by the application can be taken by the calf as soon as diarrhea occurs, helps the calf to supplement qi and consolidate the exterior, support the healthy qi and eliminate the pathogenic factors, and improve the immunity, and then, while the feeding quality is ensured, the traditional Chinese medicine composition has the effects of increasing the weight of the calf, improving anemia of the calf, improving the liver and kidney functions of the calf, and regulating and enhancing the immunity.

The invention discloses a traditional Chinese medicine composition for regulating qi and relieving distension as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The composition is prepared from the following raw material medicines in parts by weight: 10-20 parts of radix bupleuri, 10-20 parts of rhizoma cyperi, 5-15 parts of pericarpium citri reticulatae viride, 5-15 parts of ligusticum wallichii, 10-30 parts of radix paeoniae alba, 10-20 parts of semen raphani, 5-15 parts of pericarpium citri reticulatae, 5-15 parts of fructus aurantii immaturus, 5-15 parts of cortex magnoliae officinalis and 5-15 parts of fingered citron. The preparation method comprises the following steps: drying, mixing and crushing the raw material medicines, and sieving with a 80-100-mesh sieve to obtain medicine powder; before use, the medicine powder and ginger juice are mixed into paste, and the paste is applied to the Shenque acupoint. The traditional Chinese medicine application composition has the effects of soothing liver and regulating qi, invigorating spleen to promote digestion and promoting qi circulation and removing stagnation, clinical tests show that the traditional Chinese medicine application composition has a remarkable curative effect on qi stagnation type abdominal distension, can effectively reduce the VAS score of the abdominal distension and improve the symptoms such as belching and vector qi, and is safe and convenient to use and free of skin irritation.

The present application belongs to the technical field of aquaculture, and particularly relates to a plant source composite preparation for reducing antibiotic residues in cultured aquatic products, and a preparation method and use method thereof. The plant source composite preparation comprises the following raw materials in parts by weight: 20.0-35.0 parts of a composite plant extract, 0.5-2.0 parts of glucose oxidase, and 0.8-3.0 parts of bile acid; wherein the composite plant extract comprises extract of dried orange peel, extract of licorice, extract of sea buckthorn, extract of sophora fruit, extract of mulberry leaf, extract of bupleurum, and extract of green tea. The plant source composite preparation is prepared by stepwise mixing, and then mixed with feed at a ratio of 0.4-1:100 and fed for 5-10 days, so as to accelerate the metabolism and excretion of antibiotics, shorten the drug holiday, and ensure the quality and safety of aquatic products.

The present application relates to a kind of traditional Chinese medicine technical field, especially to a kind of antiviral traditional Chinese medicine composition, antiviral traditional Chinese medicine formula granule and its preparation method and application.The antiviral traditional Chinese medicine composition includes the following weight parts of component: 8 parts to 18 parts of Magnolia officinalis, 10 parts to 20 parts of Trichosanthes, 5 parts to 15 parts of Scutellaria, 5 parts to 15 parts of Bupleurum, 2 parts to 10 parts of Atractylodes, 2 parts to 10 parts of Ginger, 2 parts to 10 parts of North Adenophora, 3 parts to 11 parts of Red Shaoyao.The antiviral traditional Chinese medicine composition of the present application has good antiviral effect.

The present application relates to the technical field of oral liquid, in particular to a kind of oral liquid for preventing and treating perinatal energy metabolism disorder disease of dairy cow and preparation method thereof, it is characterized in that, the oral liquid for preventing and treating perinatal energy metabolism disorder disease of dairy cow is made of the following components, by weight parts: artemisia capillaris 200~300 parts, gardenia 150~200 parts, bupleurum 100~150 parts, polygonatum 50~100 parts, radix paeoniae alba 50~100 parts, liquorice 10~50 parts, nicotinic acid 1~10 parts, propylene glycol 200~300 parts, fructose diphosphate sodium 1~10 parts, pure water 200~400 parts.The oral liquid for preventing and treating perinatal energy metabolism disorder disease of dairy cow can prevent and treat ketosis, fatty liver and liver damage at the same time, comprehensively improve the constitution of new cow, improve immunity, improve physical indicators and reproductive performance, increase economic benefits.

The invention discloses a traditional Chinese medicine composition for treating perimenopausal dry eyes and a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine formulas. The traditional Chinese medicine composition for treating the perimenopausal dry eye comprises herba epimedii, prepared rehmannia root, rhizoma anemarrhenae, cortex phellodendri, radix paeoniae alba, angelica sinensis, radix bupleuri, poria cocos and honey-fried licorice root. The traditional Chinese medicine composition for treating perimenopausal dry eye, namely the kidney-warming dry eye formula, has the functions of warming kidney, nourishing yin, soothing liver and strengthening spleen, can effectively improve perimenopausal symptoms such as dry eye, photophobia, asthenopia, foreign body sensation, burning sensation, soreness and weakness of waist and knees, dysphoria, irritability, hot sweating, insomnia, amnesia and the like of patients with perimenopausal dry eye, improves the estrogen level, and has the efficacy of treating perimenopausal dry eye. The ocular surface inflammatory response is inhibited, and the tear film stability is improved.

The invention discloses an anti-cervical cancer nano targeting tablet based on a traditional Chinese medicine compound and a preparation method thereof. The nano targeting tablet is prepared from the following raw materials in parts by weight: centipede, bungarus parvus, syngnathus, scorpio, nidus vespae, leech, ground beeltle, frankincense, myrrh, dandelion, rhizoma cyperi, radix bupleuri, fructus aurantii, cortex phellodendri, angelica sinensis, radix curcumae, poria cocos, bighead atractylodes rhizome, radix paeoniae alba, cortex albiziae, Chinese yam, oldenlandia diffusa and selfheal. According to the invention, active ingredients of the traditional Chinese medicinal materials are retained and enriched to the maximum extent through a modern extraction technology, the curative effect of the original formula is ensured to be retained to the maximum extent, and through a folic acid receptor, CD44 receptor and pH sensitive triple targeting mechanism, the medicine is enriched in cervical cancer tissues, the local medicine concentration is improved, the system toxicity is reduced, and accurate targeting delivery is realized.

The invention discloses a pharmaceutical composition for adjuvant therapy of nodular diseases and a preparation method thereof, and relates to the technical field of medicines.The pharmaceutical composition is prepared from, by weight, 1-10 parts of cordycepin, 10-20 parts of spica prunellae extract, 10-30 parts of plant extract for softening hardness to dissipate stagnation and 15-30 parts of functional auxiliary materials; the cordyceps nucleoside is a nucleoside component of which the purity is greater than or equal to 98%; the mass ratio of oleanolic acid to ursolic acid in the prunella vulgaris extract is 3: 1, and the total content of oleanolic acid and ursolic acid is greater than or equal to 12%; the hard lump resolving plant extract is an extract of thunberg fritillary bulb, radix scrophulariae, oyster powder, seaweed, kelp, radix curcumae and radix bupleuri; the functional auxiliary material is formed by compounding at least three of a filling agent, a disintegrating agent, a suspending aid and an adhesive according to an equal mass ratio; the multiple raw materials are scientifically proportioned to play a synergistic role, the preparation process is standardized, active ingredients are reserved, indexes of finished products are stable, and large-scale production and adjuvant therapy of the nodular diseases can be achieved.

The invention belongs to the technical field of bioengineering, and particularly relates to a DNA fingerprint spectrum database for identifying stamen choerospondias and application of the DNA fingerprint spectrum database. According to the invention, the genome is subjected to enzyme digestion by using the combined restriction enzyme, and the DNA fragments of 314-394 bp are specifically selected, so that an efficient and stable library building scheme is established. According to the scheme, the development efficiency and quality of the molecular marker are remarkably improved, and a reliable basis is provided for large-scale population genetic analysis. The method can be further used for constructing DNA fingerprint databases of different species and screening core germplasm resources, and provides key technical support for germplasm identification and breeding research.

The invention relates to the technical field of traditional Chinese medicine, and particularly discloses a traditional Chinese medicine composition for treating different syndrome types of stomach diseases and a preparation method thereof.The traditional Chinese medicine composition is prepared from, by weight, 15-25 parts of fried szechwan chinaberry fruit, 8-12 parts of radix bupleuri and 15-35 parts of fried fructus perillae. The traditional Chinese medicine composition is prepared from 40-60 parts of trogopterus dung, 15-35 parts of herba patriniae, 8-12 parts of radix scutellariae, 15-25 parts of radix codonopsis, 30-50 parts of fructus jujubae, 15-25 parts of radix paeoniae alba, 15-25 parts of pericarpium citri reticulatae, 2-6 parts of radix et rhizoma rhei, 8-12 parts of radix glycyrrhizae preparata and 10-35 parts of herba agrimoniae. The traditional Chinese medicine further comprises 15-35 parts of cuttlebone, 3-5 parts of calcined concha arcae, 40-60 parts of trogopterus dung, 8-12 parts of roasted rhizoma atractylodis macrocephalae, 15-25 parts of poria cocos, 4-8 parts of Chinese prickly ash, 8-12 parts of fructus amomi, 8-12 parts of fructus amomi rotundus and 5-10 parts of fructus evodiae. A complementary treatment system is formed for the four clinical common syndromes of spleen and stomach qi deficiency and water dampness, cold-dampness and qi stagnation and cold-heat intermingling, liver-stomach disharmony, cold-heat intermingling type reflux disease and phlegm-blood stasis type gastric polyp respectively and accurately.

The invention belongs to the technical field of quality control of traditional Chinese medicine preparations, and relates to a method for determining the content of ester saikosides a and d in radix bupleuri, which comprises the following steps of: performing ultrasonic treatment on a radix bupleuri sample to be detected by taking methanol as a solvent to obtain a test solution I; performing heating reflux extraction on a radix bupleuri sample to be detected by taking methanol containing ammonia water as a solvent to obtain a test solution II; respectively detecting the contents of saikoside a and saikoside d in the test solution I and the test solution II by adopting a high performance liquid chromatography-ultraviolet detector; and calculating the difference between the two values to obtain the contents of ester-type saikosaponin a and d. According to the method, a high performance liquid chromatography-ultraviolet detector is adopted for determining the content of saikoside a and saikoside d after free and ester saikoside in radix bupleuri encounters alkali and ester bonds are broken and hydrolyzed, the content of the ester saikoside is obtained indirectly through a differential method, and the problem that quantitative determination can still be carried out under the condition that no reference substance exists is solved; a reference is provided for deeply researching chemical components of the radix bupleuri medicinal material.

This invention discloses an auxiliary device for thin-layer chromatography identification of Bupleurum chinense leaves, relating to the field of traditional Chinese medicine detection technology. It includes a single-slot developing assembly, with a base assembly fixedly mounted at the bottom and a developing solvent containing mechanism nested at the top. A mixing drive mechanism is jointly provided on the top and inside of the developing solvent containing mechanism, and a dual triggering mechanism is provided on the outside of the mixing drive mechanism. This invention reduces the difficulty of preparing the developing solvent for Bupleurum chinense leaf thin-layer chromatography identification, eliminates the need for manual input of the developing solvent, and allows the silica gel thin-layer plate to complete pre-saturation during suspension. Therefore, it eliminates the need for separate slots for placing the developing solvent and silica gel thin-layer plate, saving manpower, reducing the operational difficulty of Bupleurum chinense leaf thin-layer chromatography identification, and improving the efficiency of Bupleurum chinense leaf thin-layer chromatography identification.

This invention provides a traditional Chinese medicine composition for treating anxiety, depression, and / or sleep disorders, its preparation method, and its application, belonging to the field of traditional Chinese medicine technology. The traditional Chinese medicine composition includes the following raw materials: *Aconitum carmichaelii* root slices, calcined magnetite, stir-fried jujube seed, prepared polygala tenuifolia root, prepared pinellia tuber, tangerine peel, stir-fried immature bitter orange, ginger-processed bamboo shavings, albizia bark, poria cocos, coix seed, bamboo leaf bupleurum root, oyster shell, licorice root, and dragon bone. The preparation method includes: first decocting the *Aconitum carmichaelii* root slices, then adding the remaining medicinal ingredients and decocting with water; concentrating the filtrate into a clear paste, adding excipients, and compressing into tablets. Animal experiments show that, compared with the model group, the traditional Chinese medicine composition of this invention can significantly increase the number of times mice enter the central region and the dwell time in the open field test, and improve the number of times mice enter the open arms of the elevated cruciate maze and the dwell time; hippocampal Nielsen staining shows that neurons are neatly arranged and cell density is well restored. This invention has the effects of warming the kidney and suppressing yang, resolving phlegm and calming the mind, and can be used to prepare drugs for treating anxiety, depression, and / or sleep disorders.

The invention discloses a preparation method and application of a traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following components in parts by weight: 10 to 15 parts of radix bupleuri, 7 to 10 parts of rhizoma chuanxiong, 7 to 10 parts of rhizoma cyperi, 15 to 20 parts of radix salviae miltiorrhizae, 25 to 30 parts of cortex albiziae, 7 to 10 parts of radix paeoniae alba and 7 to 10 parts of rhizoma atractylodis macrocephalae. The preparation method of the traditional Chinese medicine composition comprises the following steps: mixing radix bupleuri, ligusticum wallichii, rhizoma cyperi, salvia miltiorrhiza, cortex albiziae, radix paeoniae alba and bighead atractylodes rhizome with an ethanol water solution, soaking, extracting, collecting an alcohol extract, concentrating, drying, crushing and sieving the alcohol extract, thereby obtaining the traditional Chinese medicine composition. According to the preparation method of the traditional Chinese medicine composition, the transfer rate of the total amount of the index components is high.

The invention relates to the technical field of traditional Chinese medicine preparations, and particularly discloses a healthy energy supporting, yang warming and meridian dredging paste which is technically characterized by being prepared from honey-fried ephedra, wine-processed cornus, safflower, red paeony root, stevia rebaudiana, prepared common monkshood daughter root, Chinese yam, kudzuvine root, prepared rhizome of rehmannia, platycodon grandiflorum, pollen typhae, ligusticum wallichii, angelica sinensis, radix achyranthis bidentatae, astragalus membranaceus, asarum, radix bupleuri, liquorice, peach kernel, lignum dalbergiae odoriferae and fructus aurantii according to a specific weight part ratio. According to the ointment formula, the theory of Zheng method of Yandezhi teaching professor and the experience of Radix Astragali-Radix Puerariae formula of Guizhongguang national medicine master are fused, a treatment system which warms yang, benefits qi, promotes blood circulation, removes blood stasis, strengthens the body resistance and dredges collaterals is established, and the ointment formula accurately aims at the core pathogenesis of yang deficiency and blood stasis type coronary heart disease angina pectoris and chronic heart failure. Clinical tests prove that the ointment is remarkably superior to conventional treatment (Plt; 0.05) in the aspects of improving traditional Chinese medicine syndromes and improving heart functions, and is good in safety; moreover, the preparation is an oral ointment, is moist and sweet in quality, is convenient to take for a long time, and has remarkable clinical application value and popularization prospect.

The present application relates to a kind of traditional Chinese medicine composition for treating calculus, raw material is: walnut kernel 25-35 parts, black fungus 10-20 parts, giant knotweed 15-25 parts, powder 25-35 parts of yam, sea gold sand 25-35 parts, 10-20 parts of yujin, 25-35 parts of huashi grass, 25-35 parts of shaddock, 10-20 parts of goose tube stone, 25-35 parts of yiwren, 10-20 parts of magnolia officinalis, 25-35 parts of xuanming powder, 10-20 parts of rhubarb, 10-20 parts of plantago, 10-20 parts of bupleurum, 10-20 parts of small chrysanthemum, 5-10 parts of cumin, 10-20 parts of Sichuan donkey's hide, 5-10 parts of raw licorice.The present application has the effect of clearing heat and promoting bile secretion, dissolving stone and stopping bleeding cooling blood, through the concept of dissolving stone and expelling stone, large stone is small, small stone is small, gallstones are discharged with bile into intestine, kidney stones, ureter and bladder stones are discharged with urine, adjust liver, gallbladder, kidney and other qi stagnation, and be supplemented with constitution conditioning syndrome differentiation treatment, it has good curative effect on various stones of liver, gallbladder, kidney and urinary tract, and it is both the treatment of principle and secondary, relieve the suffering of the majority of patients.

The present application relates to the technical field of bupleurum tissue culture, and discloses a bupleurum tissue culture device and culture method, which comprises an exhaust main mechanism, a cabinet door mechanism is movably installed on the surface of the exhaust main mechanism, a pump liquid mechanism is installed in the cabinet door mechanism, constant temperature heating mechanisms are installed inside the two sides of the exhaust main mechanism, and a light source mechanism is installed inside the back side of the exhaust main mechanism. The present application can pump the black oily liquid in the cabinet door mechanism through the pump liquid mechanism when observing, so that the inside of the liquid guide hollow glass plate is filled with nitrogen, the light transmission of the liquid guide hollow glass plate is ensured, and observation can be performed. When culturing, the inside of the liquid guide hollow glass plate is filled with black oily liquid pushed by the pump liquid mechanism, so that external light is difficult to penetrate, the device is ensured to be illuminated by the internal light source mechanism, so that light pollution from the outside is prevented from shining into the device during non-observation time, and the control effect of internal illumination variables is improved.