The invention relates to the technical field of measurement of effective components in traditional Chinese medicines, and discloses a derivative
synchronous fluorescence method for rapidly measuring the content of
flavonoid substances in a Chinese herbal
medicine extract, the most important parameter of the
synchronous fluorescence technology is selection of
delta lambda, and the selection directly affects the sensitivity and selectivity of the method; in order to select a proper
delta lambda in value,
synchronous fluorescence scanning is carried out on the sample liquid and the
flavonoid substance
system every 10 nm in the interval of 10-160 nm, and the result shows that when
delta lambda is equal to 56 nm, the
fluorescence spectrum resolutions of the sample liquid and the
flavonoid substance
system are the best. According to the derivative synchronous
fluorescence method for rapidly determining the content of the flavonoid substances in the Chinese herbal
medicine extract, first-order derivative treatment is performed on a synchronous
fluorescence spectrum, so that the analysis capability is further improved, and rapid and accurate quantitative analysis is realized. The Chinese herbal medicines such as
abrus cantoniensis hance and the like contain a large amount of flavonoid compounds, the flavonoid content can be rapidly measured by using the method, and the method has the characteristics of high sensitivity and high efficiency.