The invention provides a fast detection method of benzo(a)
pyrene in high-fat foods, relating to a detection method of benzo(a)
pyrene, which has simple and fast operation and low cost. The method comprises the following steps of: placing a sample of high-fat foods in a ground suction
bottle, adding a
potassium hydroxide-methyl
alcohol solution and normal
hexane, extracting together with water in a
microwave oven, taking out, cooling, flushing, taking out a
clear liquid of normal
hexane after settlement and stratification,
drying by rotary
evaporation, and determining the volume by using
dichloromethane to obtain a sample solution to be detected; mapping the constant energy
synchronous fluorescence spectra of the sample solution to be detected by using a
fluorescence spectrophotometer with derivative-constant energy synchronous scanning for the identification and the rough detection of benzo(a)
pyrene; and adding a second derivation function, mapping second derivate-constant energy
synchronous fluorescence spectra, reading data by adopting a derivate baseline method, and determining the quantity of benzo(a) pyrene in the sample solution to be detected by using a continuous
standard addition method.