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56 results about "Synchronous fluorescence" patented technology
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Synchronous fluorescence is used to describe the characteristics of multicomponent samples with its outstanding advantages of spectral simplification, light scattering reduction, and selectivity improvement over traditional fluorescence spectroscopy [25].
Spatial multichannel fiber coupler with laser induced synchronous fluorescence detection
A fiber coupler for synchronous test of spatial multi-channel aser adjust fluorescence inclues a laser beam coupled into an exciting fiber with equal power via a beam split mirror and a focussing lens. The output terminal of the exciting fiber is surrounded by exciting collect probes grouped by equal quantity of fluorescent collect fibers and housed with positioning liners. The fluorescent collect fibers in linear array are put in the liner diaphragm and aligned with the slit of spectrometer with a photoelectric transducer on its exit focus surface. This invention can guarantee the same test conditions for laser adjust fluorescent test spots with different spaces including same density of exciting light energy of the test spots, same fluorescent collect spatial angles and same fluorescent spectrom density with same samples.
Owner:SHANGHAI JIAO TONG UNIV
Oil species identification method by sea oil spill concentration auxiliary auxiliary parameter fluorescence spectrum
InactiveCN101458213AMaterial testing goodsFluorescence/phosphorescenceSpectral databaseFluorescence spectrometry
The invention discloses an oil type identification method. The oil spill fluorescence spectra of serial concentrations in a certain concentration range are taken as fingerprint identification indexes to identify oil types, and can be considered as a traditional fluorescence spectrum plus a dimensionality that is the concentration so as to obtain more fluorescence signals of an oil spill sample, thus improving capability of identifying the oil types. The identification method comprises the following steps: half-diluting the normal oil spill sample with a certain fixed original concentration to prepare extraction liquids of the serial concentrations, and measuring the fluorescence spectra of the extraction liquids; selecting a 3D fluorescence spectrum or a synchronous fluorescence spectrum according to requirements for detection precision and data processing speed, thus establishing a concentration parameter supplemental characteristic spectrum database of the normal oil spill samples of different oil sources sequentially. While identifying the oil type of a certain oil spill sample, the oil spill sample is extracted and diluted in the same way, and the corresponding characteristic spectrum is obtained and identified by being compared with the sample characteristic spectrum in the database, thus the optimal matched sample is the oil type of the oil spill sample.
Owner:WEIFANG UNIVERSITY
Method for rapidly detecting hogwash oil by synchronous fluorescence spectrum
InactiveCN102749315AThe result is accurateGood reproducibilityFluorescence/phosphorescenceFluorescenceSynchronous fluorescence
The invention relates to a method for rapidly detecting hogwash oil by synchronous fluorescence spectrum. The method is characterized by comprising the following steps of: preparing a sample; acquiring the synchronous fluorescence spectrum; establishing a model; and judging the unknown sample. Compared with the prior art, the method for rapidly detecting the hogwash oil by the synchronous fluorescence spectrum is based on a support vector machine algorithm, adopts the synchronous fluorescence spectrum technology, and establishes the judgment model judge the hogwash oil without pretreatment of the sample; no other chemical reagents are consumed during testing, the testing time is only 2-3 minutes, and the testing result is accurate and has good repeatability.
Owner:CHUZHOU UNIV
Synchronous fluorescence spectroscopy characteristic wavelength screening method based on particle swarm optimization algorithm
InactiveCN104062273AHigh precisionHigh speedBiological modelsFluorescence/phosphorescenceScreening methodSpectroscopy
The invention discloses a synchronous fluorescence spectroscopy characteristic wavelength screening method based on a particle swarm optimization algorithm. The synchronous fluorescence spectroscopy characteristic wavelength screening method comprises the steps of (1) analyzing a three-dimensional synchronous fluorescence spectrum by a parallel factor analysis method so as to determine an optimal wavelength difference delta(lambda) for detecting antibiotics; (2) performing spectral denoising preprocessing and spectral normalization on the synchronous fluorescence spectrum under the optimal wavelength difference delta(lambda); (3) screening out k synchronous fluorescence spectrum characteristic wavelengths by the particle swarm optimization algorithm; and (4) optimizing a kernel function parameter (c, g) of a support vector regression (SVR) model by the particle swarm optimization algorithm so as to construct an SVR forecasting model based on the particle swarm optimization algorithm. The synchronous fluorescence spectroscopy characteristic wavelength screening method based on the particle swarm optimization algorithm is beneficial to improving the precision and the speed of a model for forecasting antibiotic residues in food such as meat and meat products, and a novel method is provided for detecting the antibiotic residues in the food such as the meat and the meat products.
Owner:JIANGXI AGRICULTURAL UNIVERSITY
Fluorophototmeter capable of detecting light absorption or light transmission
The invention provides a fluorophototmeter capable of detecting light absorption or light transmission, which is improved on the basis of the original fluorophototmeter; the fluorescence detection and light absorption or light transmission detection are combined to achieve the purposes that not only the sample fluorescence spectrum, such as three-dimensional fluorescence and synchronous fluorescence can be detected, but also the absorption spectrum of the detection sample to the light, such as light absorbency or light transmittance and the like can be detected; and meanwhile, the fluorophototmeter can conveniently realize correction to the three-dimensional fluorescence spectrum. On the basis of the existing fluorophototmeter, by simply adding a reflecting system or a new detector, the fluorophototmeter can detect the transmission light transmitting a sample cell, and realizes the purposes of the invention.
Owner:CHINESE RES ACAD OF ENVIRONMENTAL SCI
Method and device for quickly detecting hogwash oil based on multi-source information fusion
InactiveCN103913435AQuick checkNon-destructiveFluorescence/phosphorescenceFluorescence spectrometryLaser-induced breakdown spectroscopy
The invention discloses a method and a device for quickly detecting hogwash oil based on multi-source information fusion. The method comprises the following steps: sequentially collecting a near-infrared spectrum, a three-dimensional synchronous fluorescence spectrum and a laser-induced breakdown spectrum of a sample; detecting the content of cholesterol and the content of triacylglycerol polymer in the sample according to information of the near-infrared spectrum, detecting the content of benzopyrene in the sample according to information of the three-dimensional synchronous fluorescence spectrum and detecting the content of heavy metal in the sample according to information of the laser-induced breakdown spectrum; and respectively comparing the contents of cholesterol, triacylglycerol polymer, benzopyrene and heavy metal of the sample with determined thresholds, and determining the sample as the hogwash oil when one content is greater than the determined threshold. The method can be used for quickly distinguishing and detecting the hogwash oil from different sources in the field.
Owner:JIANGXI AGRICULTURAL UNIVERSITY
A fluorescent mesoporous silicon oxide nanomaterial and its preparation method and application
InactiveCN102295926AGood dispersionUniform pore sizePharmaceutical non-active ingredientsIn-vivo testing preparationsDiseaseFluorescence
The invention discloses a fluorescent mesoporous silicon oxide nanomaterial and a preparation method and an application thereof. The material is characterized in that a silicon-rich microdomain formed by silicon clusters or silicon quantum dots is embedded in a framework of mesoporous silicon oxide. The preparation method of the material comprises the following steps: adding a water-soluble surfactant and a hydrolyst in water, agitating at 4-60 DEG C and dissolving fully; adding hypoxic silicate ester in the system, continuing agitating for 1-48 h, centrifuging, washing and drying; and calcining in inert atmosphere or vacuum condition to obtain a precursor material. As the fluorescent mesoporous silicon oxide nanomaterial prepared by the preparation method disclosed by the invention has good dispersibility, uniform nanometer size and adjustable aperture and adjustable luminescent performance, the fluorescent mesoporous silicon oxide nanomaterial integrates porous structure and fluorescence function, has double functions of drug loading and conveying and synchronous fluorescence imaging, and has potential application value in synchronous diagnosis and treatment of diseases.
Owner:SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
Auto-synchronous fluorescence detection method and apparatus
An apparatus for time-gated fluorescence or luminescence detection includes gating means (206) arranged to alternately permit light from an excitation source (242) to be directed to a sample (235) along a first communication path (231, 232), and then permit light emitted from the sample to be directed to a detector (246) along a second communication path (237, 238) while blocking the first communication path (231, 232): The gating means (206) may comprise a single chopper wheel or apertured disc, or a rotating or oscillating arm, and may further comprise one or more reflective facets (207). The gating means (206) may be driven via a magnetic rotor, with a ferrite bead placed to offset rotor magnets with respect to drive coils, when at rest, so as to assist with self starting.
Owner:CONNALLY RUSSELL
Method for measuring oil content of debris and core in oil logging
InactiveCN1632534AReduce workloadResolve discontinuityPreparing sample for investigationFluorescence/phosphorescenceSynchronous fluorescencePetroleum
This invention relates to a measurement method of the rock bits oil content in oil well, which comprises the following steps: first to choose the best simultaneous scanning wavelength difference ªñª™ in the same sample area; then to collect the simultaneous fluorescence spectrums within the density measurement range by this fix ªñª™; Then to make the insert process of the data by use of general math process software to form continuous three-dimension density simultaneous fluorescence spectrum database; to separately compare the simultaneous spectrum of the density sample to be measured with the spectrum data of the least square method, wherein, the density of the spectrum data of the least difference is the relative rock core or rock bits oil content.
Owner:OCEAN UNIV OF CHINA
Fast detection method of benzo(a) pyrene in high-fat foods
InactiveCN101881731AShorten the timeEasy to operateFluorescence/phosphorescencePotassium hydroxideEvaporation
The invention provides a fast detection method of benzo(a) pyrene in high-fat foods, relating to a detection method of benzo(a) pyrene, which has simple and fast operation and low cost. The method comprises the following steps of: placing a sample of high-fat foods in a ground suction bottle, adding a potassium hydroxide-methyl alcohol solution and normal hexane, extracting together with water in a microwave oven, taking out, cooling, flushing, taking out a clear liquid of normal hexane after settlement and stratification, drying by rotary evaporation, and determining the volume by using dichloromethane to obtain a sample solution to be detected; mapping the constant energy synchronous fluorescence spectra of the sample solution to be detected by using a fluorescence spectrophotometer with derivative-constant energy synchronous scanning for the identification and the rough detection of benzo(a) pyrene; and adding a second derivation function, mapping second derivate-constant energy synchronous fluorescence spectra, reading data by adopting a derivate baseline method, and determining the quantity of benzo(a) pyrene in the sample solution to be detected by using a continuous standard addition method.
Owner:XIAMEN UNIV
Homogeneous immunoassay based method for synchronous fluorescence detection of multiple disease markers
The invention relates to interdisciplinary fields of biology, medicine, material, chemistry and virology, and discloses a homogeneous immunoassay based method for synchronous fluorescence detection of multiple disease markers. The method comprises the specific steps of: first, covalently coupling different fluorescent dyes with antibodies of different disease markers, and dissolving in water or other solvents to obtain a reaction solution A; second, mixing the solution A with a graphene oxide solution to obtain a reaction solution B; third, mixing the reaction solution A, the reaction solution B and water or other solvents to obtain a solution C; and fourth, adding an antigen solution into the mixed solution C, transferring the mixed solution to a fluorescence cuvette, measuring synchronous fluorescence intensity, and conducting quantitative analysis and multi-index analysis and diagnosis. The method has advantages of simpleness, rapidness, high sensitivity, high specificity and low cost, and can realize high-throughput detection, multiple index analysis diagnosis and remote diagnosis.
Owner:WUHAN UNIV
Simultaneous and rapid detection method for benzo(a)pyrene, benzo(k)fluoranthene and anthracene in tea
InactiveCN101876638AReduce processing timeReduce lossesPreparing sample for investigationFluorescence/phosphorescenceFluorantheneSynchronous fluorescence
The invention discloses a simultaneous and rapid detection method for benzo(a)pyrene, benzo(k)fluoranthene and anthracene in tea, relating to a tea detection method. The method comprises the following steps of: grinding tea, carrying out ultrasonic extraction by adding n-hexane, separating to obtain a supernate, and carrying out ultrasonic extraction by adding dimethylsulfoxide to separate a dimethylsulfoxide extraction liquid to obtain a solution sample to be tested; scanning a first-order derivative non-linear variable angle synchronous fluorescence spectra of benzo(a)pyrene, benzo(k)fluoranthene and anthracene in the solution sample to be tested according to a scanning path by using a fluorospectro photometer with a derivative variable angle synchronous scanning function; reading a peak value with a scanning sequence between 84 and 87 for the benzo(a)pyrene, reading a peak value with a scanning sequence between 365 and 372 for the benzo(k)fluoranthene and reading the absolute value of positive and negative peak values with a scanning sequence between 930 and 1050 for the anthracene; and quantificationally calculating the concentrations of the benzo(a)pyrene, the benzo(k)fluoranthene and the anthracene in the tea solution to be tested by adopting a continuous standard adding method or a method for matching a matrix with a calibration curve.
Owner:XIAMEN UNIV
Preparation method for monodispersed and low-biotoxicity gold nanorods, and use for detection of allergen
ActiveCN103884693ADouble layer structure is stableAvoid destructionFluorescence/phosphorescenceWater bathsGold nanorod
The invention belongs to the chemical field, and provides a preparation method for monodispersed and low-biotoxicity gold nanorods, and use for detection of allergen. The preparation method comprises the steps of (1) adding cetyltrimethylammonium bromide (CTMBA) in a beaker, adding HAuCl4 and NaBH4 and stirring; (2) adding a growth solution and an anionic surfactant into the beaker, adding HAuCl4 and ascorbic acid with stirring, adding gold seeds prepared by the step (1), heating in a water bath, cooling, and filtering precipitated CTMAB; (3) taking the gold nanorods prepared by the step (2), adding a PBS solution and a probe DNA and putting in a refrigerator; and (4) taking a certain amount of the modified gold nanorods prepared by the step (3), adding a target DNA, measuring synchronous fluorescence and calculating the content of allergen according to a regression curve. According to the preparation method, the anionic surfactant is used as an additive, so that the usage amount of CTMAB is reduced. The prepared gold nanorods have good monodispersity and low biotoxicity. By using the gold nanorods in the detection of allergen, a detection limit and sensitivity of the method is obviously higher than that of a conventional technology.
Owner:JIANGNAN UNIV
Fluorescence spectrum identification method of Fushunliao schisandra chinensis
The invention discloses a fluorescence spectrum identification method of Fushunliao schisandra chinensis. The fluorescence spectrum identification method comprises the following steps of: firstly, obtaining a three-dimensional fluorescence spectrum of the Fushunliao schisandra chinensis and deriving the three-dimensional fluorescence spectrum in a data file ASCII (American Standard Code for Information Interchange) format to obtain original data of the three-dimensional fluorescence spectrum; extracting constant wavelength synchronous fluorescence signal data of emission wavelength and excitation wavelength with different wavelength intervals; obtaining a synchronous dynamic spectrum disturbed by different wavelength intervals between the related emission wavelength and excitation wavelength according to the data, so as to establish a two-dimensional related synchronous fluorescence spectrum of the Fushunliao schisandra chinensis; establishing a two-dimensional related synchronous fluorescence spectrum of unknown schisandra chinensis of the source area according to the same method; and comparing the two-dimensional related synchronous fluorescence spectrum with the two-dimensional related synchronous fluorescence spectrum of the Fushunliao schisandra chinensis to judge whether the sample is the Fushunliao schisandra chinensis. According to the method disclosed by the invention, the Fushunliao schisandra chinensis which is used as a geographical indication product and other non-Fushunliao schisandra chinensis can be directly and visually identified to enhance the protection on the Fushunliao schisandra chinensis.
Owner:HEBEI UNIVERSITY
Method for simultaneously and quickly detecting benzo[a]pyrene (BaP) and benzo[k]fluoranthene (BKP)
A method for simultaneously and quickly detecting benzo[a]pyrene (BaP) and benzo[k]fluoranthene (BKP) is characterized in that normal hexane is used as a solvent to prepare 16 kinds of 0.025ppm PAHs standard samples; a fluorescence spectrophotometer is used for scanning under the condition that the delta lambada is equal to 137nm, which is obtained from experimental optimization; only the fluorescence spectrograms with higher resolution of the BaP and BKF appear within the range of 230-350nm of emission wavelength; the synchronous fluorescence peak corresponds to the BaP in the position of 265.79nm while to the BKF in the position of 291.85nm. As the fluorescence spectrograms of the BaP and BKF overlap, the Lambert-Beer law can be adopted to derive the quantitative relation between the fluorescence intensity and the concentration of the two substances to obtain the equations set as above. Qualitative and quantitative detection is carried out on the BaP and BKF according to the fluorescence spectrograms of the BaP and BKF, which are obtained through scanning of the fluorescence spectrophotometer under the condition that the delta lambada is equal to 137nm and the equations set.
![Method for simultaneously and quickly detecting benzo[a]pyrene (BaP) and benzo[k]fluoranthene (BKP)](https://images-eureka.patsnap.com/patent_img/b02de9bb-6793-443b-89de-a047a12f51c4/A2009100135810009H1.PNG "Method for simultaneously and quickly detecting benzo[a]pyrene (BaP) and benzo[k]fluoranthene (BKP)")
![Method for simultaneously and quickly detecting benzo[a]pyrene (BaP) and benzo[k]fluoranthene (BKP)](https://images-eureka.patsnap.com/patent_img/b02de9bb-6793-443b-89de-a047a12f51c4/A2009100135810010H1.PNG "Method for simultaneously and quickly detecting benzo[a]pyrene (BaP) and benzo[k]fluoranthene (BKP)")
![Method for simultaneously and quickly detecting benzo[a]pyrene (BaP) and benzo[k]fluoranthene (BKP)](https://images-eureka.patsnap.com/patent_img/b02de9bb-6793-443b-89de-a047a12f51c4/A2009100135810002C1.PNG "Method for simultaneously and quickly detecting benzo[a]pyrene (BaP) and benzo[k]fluoranthene (BKP)")
Owner:DALIAN MARITIME UNIVERSITY
Method for synchronous fluorescence detection of chloramphenicol based on Cu/UiO-66 metal organic framework quenching
ActiveCN111398235AQuenching is strongDouble quenchingFluorescence/phosphorescenceLuminescent compositionsFluoProbesMetal-organic framework
The invention relates to the field of chemical sensors, in particular to a synchronous fluorescence detection method for chloramphenicol based on Cu / UiO-66 metal organic framework quenching. The method comprises the following steps: 1, dissolving a chloramphenicol aptamer fluorescent probe marked with a fluorophore in a buffer solution to prepare a reaction solution A; 2, uniformly dispersing a copper-zirconium bimetal organic framework material Cu / UiO-66 into a buffer solution to obtain a reaction solution B; 3, mixing the prepared reaction solution A and reaction solution B with a proper amount of buffer solution to obtain a mixed solution C; and 4, adding a certain amount of a solution with chloramphenicol content to be detected into the mixed solution C, reacting for a period of time,and detecting the fluorescence intensity through synchronous fluorescence scanning to realize quantitative detection of chloramphenicol. The method for detecting chloramphenicol, which is implementedby using the method, has the advantages of simplicity, rapidness, high sensitivity, strong specificity, low detection limit and the like.
Owner:HUBEI UNIV FOR NATITIES
Fluorescence detection method of polycyclic aromatic hydrocarbons (PAHs) in water body
InactiveCN102818791AShorten the timeLow pricePreparing sample for investigationFluorescence/phosphorescenceSynchronous fluorescenceSolvent
The invention relates to a fluorescence detection method of PAHs in a water body. The method includes processing a water sample, soaking a solid-phase extraction column and sieve plate with n-hexane, drying, grinding and shaking bamboo charcoal out, and adding the bamboo charcoal into the solid-phase extraction column after drying to constant weight to form a bamboo charcoal solid-phase extraction column; subjecting a detection water sample to the bamboo charcoal solid-phase extraction column and finally obtaining a test sample solution after elution through the n-hexane and nitrogen blowing; performing qualitative measurement, performing constant wavelength synchronous fluorescence spectrum mapping on the test sample solution, determining synchronous fluorescence spectrums of components, and comparing synchronous fluorescence characteristic peaks of PAH standards with tested samples for identification and rough determination of the PAHs; and performing the quantitative measurement, determining fluorescence intensity of components under the condition of the constant wavelength synchronous fluorescence spectrum mapping, performing quantitative analysis by the aid of regression equations of standard solutions through an external standard method, and calculating content of PAHs in the test sample solution. The fluorescence detection method has the advantages of being simple in device, rapid in operation, solvent-saving, accurate in quantification and the like.
Owner:LONGYAN UNIV
Simple preparation method and application of porous g-C3N4 (graphite-phase carbon nitride) nanosheet
InactiveCN108313990AImprove stabilityGood water solubilityNanotechnologyFluorescence/phosphorescenceFluorescenceCarbon nitride
The invention provides a simple preparation method and application of a porous g-C3N4 (graphite-phase carbon nitride) nanosheet. The simple preparation method comprises the following steps of (1) mixing dicyandiamide and ammonia chloride according to a certain mass ratio, and heating for 2 to 4h at the temperature of 500 to 600 DEG C, so as to obtain a yellow solid of g-C3N4; grinding into powder;(2) adding 50 to 200mg of g-C3N4 powder into 150 to 300mL of solvent, treating for 0.5 to 2h by ultrasonic waves, centrifuging for 5min at the rotation speed of 5000r / min, and removing the large-sizeg-C3N4, so as to obtain a white stable porous g-C3N4 nanosheet solution. The prepared porous g-C3N4 nanosheet has good stability and synchronous fluorescence property in water; under the condition ofexistence of silver ions, the synchronous fluorescence is greatly enhanced; the synchronous fluorescence has excellent selective response to the silver ions. The preparation method has the advantagesthat the preparation method is simple and convenient, and is easy to implement; the synthesizing time is short, the cost is low, and the efficiency is high; the prepared porous g-C3N4 nanosheet can be used for analyzing and detecting trace silver ions.
Owner:GUIZHOU MEDICAL UNIV
Method for acquiring optimal variable angle synchronous fluorescent spectrum
InactiveCN1645109AEasy accessOptimal scan pathFluorescence/phosphorescenceFluorescence spectrometrySynchronous fluorescence
A method includes using obtained 3-D fluorescent spectrum data to draw out contour projection figure of 3-D fluorescent spectrum, selecting scan path, obtaining fluorescent intensity by method of 2-D and three times of convolution-interpolation to draw out a variable angle synchronous fluorescent spectrum chart, selecting scan path repeatedly to have a numbers of spectrum charts and obtaining optimum variable angle synchronous fluorescent spectrum chart and its optimum scan path by comparing those obtained charts.
Owner:OCEAN UNIV OF CHINA
Method for discriminating offshore oil spill types based on multi-dimensional chemical fingerprint quantification model
ActiveCN110907625AHigh identification accuracyImprove reliabilityComponent separationColor/spectral properties measurementsAlkaneFluorescence
The invention relates to a method for discriminating offshore oil spill types based on a multi-dimensional chemical fingerprint quantification model, in particular to the method for discriminating theoffshore oil spill types based on the multi-dimensional chemical fingerprint quantification model, which can be used for quickly distinguishing fuel oil, Middle East crude oil and non-Middle East crude oil, and belongs to the field of marine environmental pollution monitoring and treatment. According to the invention, the method comprises steps of selecting n-alkanes, fluorescence characteristicsand Ni / V to construct multi-dimensional chemical fingerprints; analyzing the eight diagnosis ratios of the n-alkanes by using a partial least squares algorithm to extract three main components; carrying out 6-layer discrete wavelet transform analysis on the synchronous fluorescence spectrum by using a db7 wavelet basis, extracting 5 fluorescence information under d3, and finally screening out a first principal component of a diagnosis ratio by using an exhaustion method, and taking a wavelet coefficient of d3(332+ / -2nm) and Ni / V as modeling variables. The identification accuracy of the established model on a modeled oil sample reaches 100%, and the identification accuracy on non-modeled and weathered fuel oil and crude oil reaches 88.89% and 95.50% respectively.
Owner:DALIAN MARITIME UNIVERSITY
Method for simultaneously measuring hybrid pigment by synchronous fluorescence spectroscopy with RBF (Radial Basis Function) neural network
InactiveCN104359882AReduce overlapSimultaneous measurementBiological neural network modelsFluorescence/phosphorescenceNonlinear modelSpectroscopy
The invention discloses a method for simultaneously measuring hybrid pigment by synchronous fluorescence spectroscopy with an RBF (Radial Basis Function) neural network. The method disclosed by the invention comprises the steps of preparing for a sample, measuring by the synchronous fluorescence spectroscopy, denoising synchronous signals, establishing a nonlinear model of the RBF neural network, etc. Three-dimensional fluorescence spectrum is replaced by the synchronous fluorescence spectroscopy; with the help of the RBF neural network, spectrum can be narrowed; spectral overlap is reduced; and furthermore, synchronous measurement of hybrid pigment without any chemical separation can be realized by utilizing the nonlinear advantages of the neural network.
Owner:JIANGNAN UNIV
Bisphenol A content detecting method
ActiveCN103743715AGood forecastEliminate distractionsPreparing sample for investigationFluorescence/phosphorescenceConcentration ratioSynchronous fluorescence
The invention discloses a bisphenol A content detecting method relating to bisphenol A and provides a synchronous fluorescence spectrum and partial least squares combined bisphenol A content detecting method. The bisphenol A content detecting method comprises the following steps: (1) preparing a mixed standard solution as a sample calibration set and scanning a constant-wavelength synchronous fluorescence spectrum of the sample calibration set, wherein the mixed standard solution is composed of bisphenol A, phenol and octyl phenol in different concentration ratios; (2) establishing a quantitative calibration model between the content of the bisphenol A and the strength of the constant-wavelength synchronous fluorescence spectrum by using partial least squares; (3) predicting the artificially-synthesized sample calibration set by using the quantitative calibration model obtained in the step (2), and solving the linear fitting relationship between a predicted value of each sample and a known reference value; and (4) scanning the constant-wavelength synchronous fluorescence spectrum of the sample to be detected, and predicting through the quantitative calibration model to obtain the content of the bisphenol A in the sample to be detected. The bisphenol A content detecting method is good in effectiveness, high in reliability and good in predicting effect for the bisphenol A in the sample to be detected.
Owner:XIAMEN UNIV
Kit for detecting expression level of human ERCC1 (excision repair cross complementation 1) through PCR (polymerase chain reaction) method
InactiveCN103451303AStrong specificityEliminate non-experimental differencesMicrobiological testing/measurementFluorescence/phosphorescencePhosphateSynchronous fluorescence
Owner:JIANGSU KANGKE BIOTECH
Measurement method of biodegradable humic acid in leachate
ActiveCN106370633AAccurately predict biodegradabilityReduce processing costsFluorescence/phosphorescenceCorrelation analysisSynchronous fluorescence
A measurement method of biodegradable humic acid in leachate includes the steps of: collecting leachate samples in different bio-treatment steps; extracting and purifying leachate organic substances, and scanning the leachate organic substances to obtain infrared spectrum and fluorescent spectrum thereof; determining the number of benzene rings in and the content of the biodegradable humic acid according to parallel factor analysis of three-dimensional fluorescence spectrum of the leachate organic substances; and determining functional group composition characters of the biodegradable humic acid in the leachate through correlation analysis on synchronous fluorescent spectrum and infrared spectrum of the leachate organic substances. The method can be used for determining the composition and relative content of the biodegradable humic acid in the leachate, is simple in sample pre-treatment and has high analysis speed, and can provide the basis for technical optimization and scheme selection of treatment technology of the leachate.
Owner:CHINESE RES ACAD OF ENVIRONMENTAL SCI
Egg freshness detection device and method based on synchronous fluorescence
InactiveCN109507156ARealize non-destructive measurementFast processFluorescence/phosphorescenceFiberFluorescence
The invention relates to the technical field of egg detection, and discloses an egg freshness detection device and method based on synchronous fluorescence. The detection device comprises an underframe, a sample holder and a light shielding cover; the top of the underframe is provided with the sample holder, samples to be detected and covered by the light shielding cover are placed on the sample holder, the inferior of the said samples is a quartz glass window arranged at an opening of the top of the underframe, the lower side of the quartz glass window is vertically provided with a light source emitter and obliquely provided with a light receiving probe; the light source emitter is connected to a light source system by emitting fibers, the light receiving probe is connected to a light sensor by receiving fibers, the light source system and the light sensor are both connected to a controller, and the controller is connected to a computer. The egg freshness detection device and method have the advantages of fast and non-polluted process, nondestructive measurement of the egg freshness, low cost, convenience in operation, high quality and accuracy of detection, and potential for real-time online detection application.
Owner:山西师范大学
Spatial multichannel fiber coupler with laser induced synchronous fluorescence detection
A fiber coupler for synchronous test of spatial multi-channel aser adjust fluorescence inclues a laser beam coupled into an exciting fiber with equal power via a beam split mirror and a focussing lens. The output terminal of the exciting fiber is surrounded by exciting collect probes grouped by equal quantity of fluorescent collect fibers and housed with positioning liners. The fluorescent collect fibers in linear array are put in the liner diaphragm and aligned with the slit of spectrometer with a photoelectric transducer on its exit focus surface. This invention can guarantee the same test conditions for laser adjust fluorescent test spots with different spaces including same density of exciting light energy of the test spots, same fluorescent collect spatial angles and same fluorescent spectrom density with same samples.
Owner:SHANGHAI JIAO TONG UNIV
System and method for synchronized fluorescence capture
ActiveUS11487097B1Exact correlationAccurately correlatedTelevision system detailsColor television detailsEngineeringFluorophore
A system and method for high resolution multi-fluorescence imaging with synchronized image acquisition amongst sensors can be used to simultaneously capture fluorescence signals from multiple fluorophores over extremely large fields of view. The system can include an array of micro-cameras, along with a particular arrangement of fluorescent filters that can be fixed in one location or moved to new locations.
Owner:RAMONA OPTICS INC
Application of protein in predicting drug performance
The invention discloses application of a protein in predicting the drug performance, and belongs to the field of protein application. The protein is used for predicting the drug performance, and drugs include pesticides, human drugs and veterinary drugs. The application is implemented by the steps of preparing a 0.02 M phosphate buffer solution with the pH value of 7.4, and dissolving and diluting a protein solution by using the prepared buffer solution according to a signal value; and mixing the prepared protein diluent with a drug to be determined according to a molar ratio of 1: (1-300), and predicting the performance of the drug by using a fluorescence spectrum, or synchronous fluorescence, or three-dimensional fluorescence, or circular dichroism spectrum, or UV-Vis absorption spectrum, or linear spectrum, or banded spectrum. A BP neural network model among five indexes (a quenching constant, a binding site number, a binding constant, free energy and a binding distance) is obtained through a fluorescence spectrum and toxicity so as to predict the drug performance, and the application has important practical significance on the development of the drug research and development industry.
Owner:HEILONGJIANG UNIV
Auto-synchronous fluorescence detection method and apparatus
An apparatus for time-gated fluorescence or luminescence detection includes gating means (206) arranged to alternately permit light from an excitation source (242) to be directed to a sample (235) along a first communication path (231, 232), and then permit light emitted from the sample to be directed to a detector (246) along a second communication path (237, 238) while blocking the first communication path (231, 232): The gating means (206) may comprise a single chopper wheel or apertured disc, or a rotating or oscillating arm, and may further comprise one or more reflective facets (207). The gating means (206) may be driven via a magnetic rotor, with a ferrite bead placed to offset rotor magnets with respect to drive coils, when at rest, so as to assist with self starting.
Owner:CONNALLY RUSSELL
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