Method for synchronous fluorescence detection of chloramphenicol based on Cu/UiO-66 metal organic framework quenching
A metal-organic framework and uio-66 technology, applied in the field of chemical sensors, can solve the problems of high cost, time-consuming and laborious, cumbersome operation process, etc., and achieve the effects of strong anti-interference, improved signal-to-background ratio, and high sensitivity
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Embodiment 1
[0043] Embodiment 1 A method for synchronous fluorescence detection of chloramphenicol based on Cu / UiO-66 metal-organic framework quenching, the specific steps are as follows:
[0044] (1) The CAP aptamer fluorescent probe labeled with a fluorophore is synthesized by a DNA synthesizer, and the sequence of the CAP aptamer probe used is: 5'-ACT TCA GTG AGT TGT CCC ACG GTC GGC GAG TCG GTG GTA GCC C-3', a ROX fluorescent group is attached to the 3' end, the aptamer probe is purified by high performance liquid chromatography, and its sequence correctness is checked by mass spectrometry;
[0045] (2) Dissolve the CAP aptamer fluorescent probe labeled with a fluorescent group prepared in step (1) in a Tris-HCl buffer solution with a concentration of 0.2mol / L containing 80mmol / L NaCl and a pH of 7.7, and configure it as a labeled The concentration of the CAP aptamer fluorescent probe with a fluorescent group is 2×10 -7 mol / L of reaction solution A;
[0046] (3) Cu / UiO-66 was synthes...
Embodiment 2
[0064]Embodiment 2 A method for synchronous fluorescence detection of chloramphenicol based on Cu / UiO-66 metal-organic framework quenching, the specific steps are as follows:
[0065] For the determination of the actual sample, the actual sample is first homogenized, and the fish sample is provided by the Food and Drug Administration Center, and it is confirmed that it is a fish sample completely free of chloramphenicol. Then the chloramphenicol standard solution of different concentrations is added in the fish sample of equal amount (5g) respectively, and homogenize, then, concrete steps are as follows:
[0066] The homogenized fish samples were crushed into surimi, mixed with 10 mL of ethyl acetate, and then centrifuged at 5000 r / min for 5 min to collect the supernatant. Then add 10 mL of ethyl acetate to the residue again for homogenization, repeat twice, transfer all the collected supernatant to a vial, blow dry with nitrogen, and then wash with a solution containing 10 v / ...
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