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Method for synchronous fluorescence detection of chloramphenicol based on Cu/UiO-66 metal organic framework quenching

A metal-organic framework and uio-66 technology, applied in the field of chemical sensors, can solve the problems of high cost, time-consuming and laborious, cumbersome operation process, etc., and achieve the effects of strong anti-interference, improved signal-to-background ratio, and high sensitivity

Active Publication Date: 2020-07-10
HUBEI UNIV FOR NATITIES
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  • Abstract
  • Description
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Problems solved by technology

[0003] At present, the qualitative and quantitative detection methods for antibiotic drug molecules mainly include chromatography and spectrometry, but most of these methods rely on the combination of multiple instruments, and the final data need to be calculated after complicated combinations. Get the type and content of the substance to be tested
Although the accuracy of the method of instrumentation is relatively high, such as the determination of chloramphenicol residues in animal-derived foods in the national standard method GB T 22338-2008 in the prior art, gas chromatography-mass spectrometry, liquid The detection limit of chloramphenicol by phase chromatography-mass spectrometry is 0.1 μg / kg, but the operation process is too cumbersome, time-consuming and labor-intensive, and the cost is relatively high, and there are certain requirements for the operator’s test level

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  • Method for synchronous fluorescence detection of chloramphenicol based on Cu/UiO-66 metal organic framework quenching
  • Method for synchronous fluorescence detection of chloramphenicol based on Cu/UiO-66 metal organic framework quenching
  • Method for synchronous fluorescence detection of chloramphenicol based on Cu/UiO-66 metal organic framework quenching

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Embodiment 1

[0043] Embodiment 1 A method for synchronous fluorescence detection of chloramphenicol based on Cu / UiO-66 metal-organic framework quenching, the specific steps are as follows:

[0044] (1) The CAP aptamer fluorescent probe labeled with a fluorophore is synthesized by a DNA synthesizer, and the sequence of the CAP aptamer probe used is: 5'-ACT TCA GTG AGT TGT CCC ACG GTC GGC GAG TCG GTG GTA GCC C-3', a ROX fluorescent group is attached to the 3' end, the aptamer probe is purified by high performance liquid chromatography, and its sequence correctness is checked by mass spectrometry;

[0045] (2) Dissolve the CAP aptamer fluorescent probe labeled with a fluorescent group prepared in step (1) in a Tris-HCl buffer solution with a concentration of 0.2mol / L containing 80mmol / L NaCl and a pH of 7.7, and configure it as a labeled The concentration of the CAP aptamer fluorescent probe with a fluorescent group is 2×10 -7 mol / L of reaction solution A;

[0046] (3) Cu / UiO-66 was synthes...

Embodiment 2

[0064]Embodiment 2 A method for synchronous fluorescence detection of chloramphenicol based on Cu / UiO-66 metal-organic framework quenching, the specific steps are as follows:

[0065] For the determination of the actual sample, the actual sample is first homogenized, and the fish sample is provided by the Food and Drug Administration Center, and it is confirmed that it is a fish sample completely free of chloramphenicol. Then the chloramphenicol standard solution of different concentrations is added in the fish sample of equal amount (5g) respectively, and homogenize, then, concrete steps are as follows:

[0066] The homogenized fish samples were crushed into surimi, mixed with 10 mL of ethyl acetate, and then centrifuged at 5000 r / min for 5 min to collect the supernatant. Then add 10 mL of ethyl acetate to the residue again for homogenization, repeat twice, transfer all the collected supernatant to a vial, blow dry with nitrogen, and then wash with a solution containing 10 v / ...

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Abstract

The invention relates to the field of chemical sensors, in particular to a synchronous fluorescence detection method for chloramphenicol based on Cu / UiO-66 metal organic framework quenching. The method comprises the following steps: 1, dissolving a chloramphenicol aptamer fluorescent probe marked with a fluorophore in a buffer solution to prepare a reaction solution A; 2, uniformly dispersing a copper-zirconium bimetal organic framework material Cu / UiO-66 into a buffer solution to obtain a reaction solution B; 3, mixing the prepared reaction solution A and reaction solution B with a proper amount of buffer solution to obtain a mixed solution C; and 4, adding a certain amount of a solution with chloramphenicol content to be detected into the mixed solution C, reacting for a period of time,and detecting the fluorescence intensity through synchronous fluorescence scanning to realize quantitative detection of chloramphenicol. The method for detecting chloramphenicol, which is implementedby using the method, has the advantages of simplicity, rapidness, high sensitivity, strong specificity, low detection limit and the like.

Description

technical field [0001] The invention belongs to the field of chemical sensors, in particular to a method for synchronous fluorescence detection of chloramphenicol based on Cu / UiO-66 metal-organic framework quenching. Background technique [0002] A series of antibiotic drug molecules such as chloramphenicol (Chloramphenicol, CAP) have good bactericidal and anti-inflammatory effects, and because of their wide application and relatively cheap price, they have been widely used in disease prevention and treatment, animal husbandry and in the aquaculture industry. However, long-term consumption of foods containing antibiotics will cause children's immunity to weaken, affect the intestinal flora, lead to malnutrition, reduce the body's resistance to pathogens, make some pathogenic bacteria resistant to drugs, and the disease is difficult to cure. In severe cases, it will cause liver and kidney damage . According to reports, due to the abuse and misuse of antibiotics, many "super...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/06C09K11/02
CPCG01N21/6428C09K11/06C09K11/02G01N2021/6432C09K2211/183C09K2211/188C09K2211/1007
Inventor 向东山鲁子敬汪鹏熊威威翟琨
Owner HUBEI UNIV FOR NATITIES
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