Simultaneous and rapid detection method for benzo(a)pyrene, benzo(k)fluoranthene and anthracene in tea

A detection method and a benzo-based technology, which are applied to the simultaneous rapid detection of benzofluoranthene and anthracene, in the field of benzopyrene in tea, can solve the problems of complex tea substrates, shorten sample processing time, reduce losses, and are economical and simple. Effect

Inactive Publication Date: 2010-11-03
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity of the tea substrate, conventional pretreatment methods ...

Method used

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  • Simultaneous and rapid detection method for benzo(a)pyrene, benzo(k)fluoranthene and anthracene in tea
  • Simultaneous and rapid detection method for benzo(a)pyrene, benzo(k)fluoranthene and anthracene in tea
  • Simultaneous and rapid detection method for benzo(a)pyrene, benzo(k)fluoranthene and anthracene in tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Detection of 3 kinds of polycyclic aromatic hydrocarbons in a commercially available Tieguanyin tea

[0040] 1) After the tea leaves are evenly ground, weigh about 1.0g of the sample into a 25mL serum bottle, add 5mL of n-hexane, ultrasonically extract for 10min, power 59KHz, 100%; repeat 3 times, combine the extracts (15mL in total) and add 10mL of dimethylformamide Sulfoxide, ultrasonically extracted for 15 minutes, the conditions are the same as above. Dilute 0.5mL of dimethyl sulfoxide extract to 2mL and measure the first-order derivative nonlinear variable-angle synchronous fluorescence spectrum on a fluorescence spectrophotometer, such as Figure 9 Show. The three components in the sample were quantitatively analyzed by continuous standard addition method, that is, 2 microliters of standard solutions of benzo(a)pyrene, benzo(k)fluoranthene and anthracene (benzo(a)pyrene) were continuously added to the sample solution. , benzo (k) fluoranthene and anth...

Embodiment 2

[0044] Example 2: Detection of 3 kinds of polycyclic aromatic hydrocarbons in 3 kinds of commercially available Tieguanyin tea leaves

[0045] Three kinds of commercially available Tieguanyin tea were purchased, the quality of which was quite different, and they were named samples No. 1, No. 2 and No. 3 respectively. After the sample is ground evenly, weigh about 0.5g into a 25mL serum bottle, add 5mL of n-hexane, ultrasonically extract for 10min, power 59KHz, 100%; repeat 3 times, combine the extracts (15mL in total) and add 5mL of dimethyl sulfoxide, Ultrasonic extraction for 15 minutes under the same conditions as above. Take 0.3mL dimethyl sulfoxide extract and dilute to 2mL and measure the first derivative variable angle synchronous fluorescence spectrum on a fluorescence spectrophotometer. The quantitative analysis method is the same as that in Example 1, using the continuous standard addition method. Three sets of experiments were performed in parallel for each sample,...

Embodiment 3

[0048] Example 3: Detection of 3 polycyclic aromatic hydrocarbons in a commercial oolong tea bag

[0049] After the tea leaves are ground evenly, weigh 0.6g into a 25mL serum bottle, add 7mL of n-hexane, ultrasonic extraction for 15min, power 59KHz, 100%; Extract for 10min, power 59KHz, 100%. Dilute 1.0 mL of dimethyl sulfoxide extract to 2 mL and measure its fluorescence value on a fluorescence spectrophotometer. The continuous standard addition method was used for quantification, and the results showed that the contents of benzo(a)pyrene, benzo(k)fluoranthene and anthracene in tea were 27.3ng / g, 80.1ng / g, and 390.3ng / g, respectively.

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Abstract

The invention discloses a simultaneous and rapid detection method for benzo(a)pyrene, benzo(k)fluoranthene and anthracene in tea, relating to a tea detection method. The method comprises the following steps of: grinding tea, carrying out ultrasonic extraction by adding n-hexane, separating to obtain a supernate, and carrying out ultrasonic extraction by adding dimethylsulfoxide to separate a dimethylsulfoxide extraction liquid to obtain a solution sample to be tested; scanning a first-order derivative non-linear variable angle synchronous fluorescence spectra of benzo(a)pyrene, benzo(k)fluoranthene and anthracene in the solution sample to be tested according to a scanning path by using a fluorospectro photometer with a derivative variable angle synchronous scanning function; reading a peak value with a scanning sequence between 84 and 87 for the benzo(a)pyrene, reading a peak value with a scanning sequence between 365 and 372 for the benzo(k)fluoranthene and reading the absolute value of positive and negative peak values with a scanning sequence between 930 and 1050 for the anthracene; and quantificationally calculating the concentrations of the benzo(a)pyrene, the benzo(k)fluoranthene and the anthracene in the tea solution to be tested by adopting a continuous standard adding method or a method for matching a matrix with a calibration curve.

Description

technical field [0001] The invention relates to a detection method of tea, in particular to a simultaneous rapid detection method of benzo(a)pyrene, benzo(k)fluoranthene and anthracene in tea. Background technique [0002] In the world, tea is the second most popular drink after water. In my country, tea is recognized as the "national drink". Tea contains more than 600 kinds of organic compounds such as caffeine, tea polyphenols, flavonoids, amino acids and vitamins, as well as 28 kinds of inorganic elements such as sodium, potassium and fluorine. At present, a large number of studies have shown that drinking tea is beneficial to health, and has the effects of anti-cancer, anti-aging and lowering blood sugar. However, in the process of tea growth, polycyclic aromatic hydrocarbons (PAHs) in the air will be enriched, and trace amounts of polycyclic aromatic hydrocarbons (PAHs) will also be formed in the production process, such as roasting and drying. PAHs are harmful to huma...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N1/28
Inventor 李耀群李秀英李呐林丽容黄维
Owner XIAMEN UNIV
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