53 results about "Spectrofluorometer" patented technology

The invention discloses a method for detecting fluorine ions in a water body based on a smart phone, and belongs to the technical field of fluorine ion detection. According to the portable detection equipment based on the smart phone, laser emitted by the laser pen is divided into two beams through the beam splitter, then a to-be-detected liquid is compared with a sample with a fixed concentration, the smart phone is used for shooting a picture, and then the RGB value of an effective area is identified, so that the G ratio of the to-be-detected liquid to the sample with the fixed concentration is obtained; according to the method, a linear relation between fluorine ions with different concentrations and G ratios is constructed, high-sensitivity detection of F <-> is realized, compared with traditional fluorospectro photometer detection, the sensitivity is higher, the technical problem of test result deviation caused by instability of a rechargeable light source used in the field detection process in the prior art is solved, and the method is suitable for large-scale popularization and application. According to the invention, on-site detection of the water sample to be detected can be realized through the smart phone.

This invention discloses a method for determining fiber microplastics in aqueous textile solutions based on fluorescence spectrophotometry, belonging to the field of textile pollutant detection technology. The method includes: S1, collecting wastewater and filtering it through a filter membrane; S2, rinsing the filter membrane with sodium hypochlorite solution; S3, rinsing the fiber microplastics on the filter membrane into a separatory funnel; S4, pouring out a portion of the fiber microplastics from the upper end of the separatory funnel and collecting another portion from the lower end; S5, repeating S1-S4, rinsing the filter membrane with hydrochloric acid during this process, and collecting the remaining fiber microplastics; S6, dyeing the three solutions obtained; S7, preparing standard series solutions using polyester, polyamide, and polypropylene standard liner; S8, measuring and plotting a standard curve of fluorescence intensity versus fiber microplastic concentration using a fluorescence spectrophotometer; S9, measuring the fluorescence intensity of the three solutions obtained in S6. This invention is applicable to the field of aqueous textile solutions, enabling quantitative analysis of unknown samples, and can analyze both single and mixed samples with minimal detection bias.

The invention discloses a cyromazine concentration detection and analysis method and system, and relates to the technical field of pesticide residue detection.The method comprises the specific steps that a cyromazine detection chip is prepared, an integrated signal probe is prepared, a detection reaction system is constructed, triple cooperative response signals are collected, multiple signals are fused, and the cyromazine concentration is calculated; the cyromazine detection chip and the integrated signal probe are prepared, the fluorescence, electrochemistry and SERS triple synergistic response signal acquisition technology is combined, the accuracy of cyromazine concentration detection is improved, the gold nanoparticle modified substrate and the gold nanorod loaded fluorescence and electrochemical active substances are utilized, the response strength of the signal probe is enhanced, and the sensitivity of the signal probe is improved. The fluorescence spectrophotometer can detect cyromazine with lower concentration, meanwhile, the electrochemical workstation and the Raman spectrometer are introduced, so that the electrochemical peak current and SERS characteristic peak displacement are accurately acquired, signals from different sources are normalized, and errors existing among single signals are eliminated.

The invention discloses an activated sludge fluorescence online detection device based on a flow cell, and relates to the field of activated sludge biomass detection.The activated sludge fluorescence online detection device comprises a fluorescence spectrophotometer and a fluorescence spectrophotometer sample table arranged on the fluorescence spectrophotometer, and the fluorescence spectrophotometer is connected with a processor through a cable; the fluorescence spectrophotometer sample table is connected with a sample cup through a guide pipe, activated sludge is arranged in the sample cup, and a peristaltic pump is arranged on the guide pipe; the activated sludge continuously flows through the fluorescence spectrophotometer sample table through the guide pipe for three-dimensional fluorescence detection through the peristaltic pump, sludge sedimentation in a traditional cuvette is avoided, measurement is simple and convenient, and on-line real-time monitoring of the activated sludge biomass can be achieved.

The invention belongs to the technical field of biology, and particularly relates to regulation and control of cinnamyl aldehyde on hyperuricemia related gene promoter region G quadruplex. According to the application disclosed by the invention, firstly, the capacity of cinnamyl aldehyde for regulating and controlling a G quadruplex in a hyperuricemia related gene promoter region is explored by utilizing circular dichroism, and the influence of cinnamyl aldehyde on regulation and control of a nucleic acid structure is further evaluated by utilizing a fluorospectro photometer, so that support is provided for a molecular mechanism of cinnamyl aldehyde for relieving hyperuricemia on a gene level. In conclusion, the invention discloses a brand new mechanism that cinnamyl aldehyde regulates and controls uric acid metabolism related genes through a targeted G quadruplex structure, a precise action target and a potential drug development direction are provided for treatment of hyperuricemia, and meanwhile, an established small molecule and G quadruplex interaction verification method provides technical reference for similar research.

The invention discloses a urease activity detection method based on a non-reversible reaction of dopamine and resorcinol. The urease activity detection method comprises the following steps: mixing a sample to be detected, a buffer solution with the pH value of 7.0 and a urea solution with a certain concentration; adding a certain amount of dopamine and resorcinol into the mixed solution, under the condition of 25-45 DEG C, catalyzing urea hydrolysis by urease, causing the pH value of the surrounding area of the urease to rise so as to form a local alkaline environment, further inducing the reaction of the dopamine and the resorcinol to generate a blue fluorescent substance, and adding excessive hydrochloric acid to terminate the reaction after the reaction is carried out for 40-120 minutes; and detecting the fluorescence intensity of the reaction liquid by using a fluorospectro photometer, and calculating the urease activity in the sample according to a fluorescence intensity-urease concentration standard curve. The urease activity detection method based on the reaction of dopamine and resorcinol has the advantages of high detection speed, high sensitivity, good selectivity and the like.

The present disclosure provides an integrating-sphere-assisted resonance synchronous (ISARS) spectroscopy method performed with an ISARS spectrophotometer, for example, a spectrofluorometer equipped with an integrating-sphere accessory. The methods and systems of the present disclosure utilize the ISARS spectrophotometer equipped with an integrating sphere to determine the ISARS spectral intensity, quantify the ISARS-based absorbance, and subsequently evaluate the sample's double-beam UV-vis absorbance spectrum (also known as the absorption extinction spectrum). This ISARS method enables quantitative separation of light absorption and scattering contribution to the sample UV-vis extinction spectrum measured with double-beam UV-vis spectrophotometer.

The invention discloses a method for detecting heavy metal ions in chemical sewage. The method comprises the following steps: 1, collecting a sample; 2, sample pretreatment, precipitate removal and pH adjustment; step 3, separating and enriching the pretreated water sample by adopting a solid-phase extraction (SPE) technology, eluting heavy metal ions by using an eluting solvent, and collecting an eluent; and 4, modifying an electrode by using the functionalized nano material, preparing a heavy metal ion sensor, and reacting the enriched sample with the sensor to detect heavy metal ions. 5, meanwhile, adding a specific fluorescent probe into the enriched liquid, and reacting at a certain temperature to form a compound of heavy metal ions and generate a fluorescent signal; measuring the fluorescence intensity by using a fluorospectro photometer, and establishing a standard curve; according to the method disclosed by the invention, high sensitivity, selectivity and rapid detection of heavy metal ions are realized by combining solid-phase extraction, multiple reactions and an electrochemical sensor technology. The operation is simple, economical and practical.

The invention provides a single-molecule fluorescent probe based on a BODIPY derivative BP-BNO2 as well as a synthesis method and application of the single-molecule fluorescent probe, and the specific Chinese name of the derivative is 5, 5-difluoro-1, 3, 7, 9-tetramethyl-10-(4-nitrophenyl)-5H-4 lambda 4, 5 lambda 4-dipyrrolo [1, 2-c: 2 ', 1'-f] [1, 3, 2] diazaborane. The invention also provides a synthesis method of the derivative and application of the derivative in detection of hypobromous acid in vitro, cells and nasal cavities of mice with allergic rhinitis. According to the method, on the basis of a derivative BP-BNO2 of BODIPY, the concentration change of hypobromous acid is detected through a fluorospectro photometer in a mixed solution of a phosphate buffer solution and acetonitrile (the volume ratio is 7: 3). The method also realizes noninvasive in-situ imaging at cell and mouse levels, and can be used for in-situ monitoring of hypobromous acid level in the nasal cavity of the mouse with allergic rhinitis and auxiliary diagnosis of allergic rhinitis.

The utility model provides a fluorescence spectrophotometer for measuring multiple parameters of water quality. The fluorescence spectrophotometer comprises a spectrophotometer main body, according to the fluorospectro photometer for water quality multi-parameter measurement, when the water quality is measured, a worker needs to adjust the position of the sample holder, so that a measured water quality sample can be aligned with a light path, and when the worker pulls the sample holder outwards through a pull ring, the sample holder can be accurately positioned due to large outward pulling force of the worker; according to the present invention, the water quality sample placed in the sample holder is easily splashed from the cuvette so as to cause the moisture in the device due to the influence of the water, or the device is corroded due to the splashing of the solution during the measurement of other solutions, and through the arrangement of the wiping cloth, when the water or the solution is splashed in the device, the wiping cloth can be removed; the sliding block is driven by the motor to slide left and right on the second sliding rod to adjust the position of the wiping cloth, and then the length of the wiping cloth is adjusted through the hydraulic cylinder, so that the wiping cloth can wipe water or a solution splashed out of the sample chamber through the sliding block and the hydraulic cylinder.

The present invention provides a blood lead detection method, and the blood lead detection method includes the following steps: (1) Mix a blood sample to be measured, a protease and a buffer solution and perform water bath, then mix with an acid and continue water bath, cool, mix with trichloroacetic acid and centrifuge, take the supernatant and adjust the pH to obtain a test solution; (2) Mix the test solution obtained in step (1) with an aqueous solution of gold nanoclusters, then perform detection using a fluorescence spectrophotometer, and calculate the blood lead concentration in the blood sample to be measured by combining with a standard curve. The blood lead detection method provided by the present invention is simple to operate, has a low detection limit, high sensitivity, and has little limitation on personnel requirements and site and instrument requirements.

The utility model discloses a monitoring and compensating structure for eliminating the influence of EX spectrum fluctuation of a fluorospectro photometer, and particularly relates to the technical field of fluorescence analysis and detection, which comprises an EM light energy beam splitter, an EM wavelength energy matching tuner, a monitoring multiplier tube, an electronic negative feedback compensation control module and a measurement multiplier tube, the EM light energy beam splitter is arranged between a sample pool in the fluorescence spectrophotometer and the EM light energy beam splitter I, the EM light energy beam splitter splits EX light energy into two paths, the light beam I in the horizontal direction enters the fluorescence measurement channel to complete fluorescence testing, and the light beam II in the vertical direction enters the EX light energy monitoring channel to monitor the fluctuation of the EX light energy. According to the utility model, a monitoring light path structure of which the photoelectric characteristic of a monitoring light path is consistent with that of a measuring light path detector is designed to be embedded into a measuring light path and a circuit of an instrument, so that the correction compensation quantity of fluctuation quantity which is equal to sample fluorescence in quantity ratio and same in phase and synchronization is obtained in time to eliminate the error influence caused by EX energy fluctuation in a fluorescence test.

The invention discloses a preparation method and device of an intelligent biosensor. The preparation method comprises the following steps: preparing mixed solutions with different concentrations, transferring the mixed solutions to a cuvette of a fluorospectro photometer, and carrying out fluorescence intensity analysis on the mixed solutions in a wavelength range of 350-500 nm; establishing a linear relation of maximum fluorescence emission peak values corresponding to different concentrations; establishing a linear relation between chromatic values corresponding to different concentrations; photographing and extracting a chromatic value by using an RGB algorithm; and comparing the linear relationship to obtain the corresponding concentration. Important data support is provided for tumor diagnosis, chemotherapy drug resistance analysis and personalized treatment. The method combines an intelligent sensing technology and a portable platform, is suitable for intelligent medical equipment and cancer biomarker detection, and has a wide application prospect.

The invention discloses an automatic navigation sea water component analysis system, and relates to the technical field of sea water component analysis, and the system comprises an upper computer which is used for controlling the initialization, preheating, cyclic detection and data storage processes of the whole system; the peristaltic pump is used for transmitting the seawater sample in the underway process into the quartz flow cell according to preset flow and time parameters; the fluorospectro photometer sample cell is made of a quartz flow cell, one end of the fluorospectro photometer sample cell is used for receiving the seawater sample transmitted by the peristaltic pump, and the other end of the fluorospectro photometer sample cell is used for discharging detected liquid; the waste liquid collecting pool is used for receiving waste liquid discharged after detection of the fluorospectro photometer, so that the purpose of replacing a sample without opening a cover to move the sample pool is achieved, the experiment efficiency is improved, human errors are reduced, the instrument can continuously extract navigation water for detection and analysis, human errors and operation errors are reduced, the efficiency is greatly improved, and the device is suitable for popularization and application. And labor and time costs are reduced.

The invention discloses a DNA (Deoxyribose Nucleic Acid) probe ATP-3 and application thereof. The DNA (Deoxyribose Nucleic Acid) probe ATP-3 disclosed by the invention comprises DOX-3 and DOX-3-CP (Chlorinated Drug detection can be realized by using the DNA probe ATP-3 based on a non-label method DNA biosensor. Based on a double-strand competitive binding mechanism (DOX-3 and DOX specific binding leads to double-strand dissociation) of a DNA probe ATP-3, and in combination with a fluorescence switching characteristic (fluorescence enhancement during double-strand binding and quenching during dissociation) of an embedded fluorescent agent DAPI, trace detection of drugs is realized. By using a fluorospectro photometer as a detection auxiliary instrument, the method has the advantages of sensitive result, simplicity in operation, lower cost and the like.

The invention discloses a method and kit for detecting hepatitis B virus DNA by a self-assembled label-free CRISPR biosensor based on signal amplification, (1) performing RCA amplification on the HBV nucleic acid sample to be detected; (2) mixing the amplified product of step (1) with Cas12a, crRNA and biotin-labeled ssDNA, incubating the reaction and then heating to inactivate Cas12a; (3) adding S1 to the reaction solution treated by step (2) for incubation and hybridization, then adding S2 for incubation and hybridization, adding streptavidin magnetic ball for oscillation incubation after completion, separating the magnetic ball after incubation, adding fluorescent dye SYBR Green I for incubation, and using a fluorescence spectrophotometer to measure the fluorescence intensity after incubation. Due to the powerful signal output performance brought by multiple signal amplification, the designed method has the advantages of high sensitivity and wide linear range.

The present invention provides a method for detecting the oil content rate of drilling fluid based on spectrometry, which solves the technical problem that when incident light hits turbidity particles during the fluorescence detection of existing drilling fluid, the turbidity particles themselves will absorb to a certain extent and scatter in all directions, resulting in the attenuation of the incident light; therefore, the light intensity irradiated onto the oil component becomes weak, thus affecting the final emitted fluorescence intensity. It can be widely applied to the field of spectral detection; specifically, it includes the following steps: mixing the drilling fluid sample with water and oscillating to obtain a solution to be measured; irradiating the solution to be measured with an ultraviolet light source, detecting the spectral image of the solution to be measured through a fluorescence spectrophotometer, and obtaining the fluorescence intensity at the corresponding wavelength according to the oil types contained in the drilling fluid; obtaining the current oil content rate according to the oil content rate - fluorescence intensity relationship model; detecting the turbidity value of the solution to be measured, and obtaining the standard oil content rate of the drilling fluid according to the turbidity compensation model.

The utility model relates to the technical field of automatic sample injectors, in particular to an automatic sample injector of an atomic fluorescence spectrophotometer, which comprises a box body, a transverse plate is fixedly connected below the inner wall of the box body, a controller is fixedly connected below one side of the box body, a shell is fixedly connected at the top of the box body, and the transverse plate is fixedly connected with the shell. A material pipe is fixedly connected to the inner wall of one side of the top of the shell, the inner wall of the bottom of the material pipe is fixedly connected with a water inlet pipe of the conveying pump, and a valve is fixedly connected to the inner wall of the lower portion of one side of the material pipe. Through cooperation of a box body, a transverse plate and a fixing structure, when the device is used, a sample tube can be placed between two clamping plates, a lead screw is rotated, the lead screw drives a sliding block to move, the sliding block drives the clamping plate on one side to move, then the two clamping plates are made to be close to each other, a rubber block is attached to the sample tube, and then the sample tube is fixed; the sample bottles with different sizes can be conveniently fixed, so that the sample injection work can be conveniently carried out on the sample bottles with different sizes.

This utility model discloses a fluorescence performance testing device for fluorescent composite fibers, relating to the field of composite fiber processing technology. The device includes a fluorescence spectrophotometer. A cover plate is connected to one side of the top of the spectrophotometer via a damping shaft. A first light-shielding strip, a second light-shielding strip, and two curved grooves are respectively provided on both sides of the cover plate. Both the first and second light-shielding strips have curved grooves and curved openings on their sides near the cover plate. A first sealing strip is connected to the bottom of the first light-shielding strip. Through the arrangement of the cover plate, adsorption block, electromagnet, first light-shielding strip, and first sealing strip, the device allows the first and second light-shielding strips to rotate during the opening and closing of the cover plate. The increased width of the cover plate allows it to directly rest on the top and surface of the fluorescence spectrophotometer, rather than being embedded inside, thus avoiding structural wear. This effectively prevents structural wear and light leakage caused by an improperly closed cover plate.

The invention belongs to the technical field of pesticide residue detection, and particularly discloses an ultraviolet-fluorescence spectrum fused cyromazine detection method based on a BOSS-PSO-BP neural network, and the method comprises the following steps: step 1, preparing a cyromazine sample with gradient concentration of 2.25 * 10 <-6 >-1.47 * 10 <-4 > mg / mL for training a neural network; 2, detecting the samples with different concentrations by using an LS55 fluorescence spectrophotometer and a UV-6300 ultraviolet spectrophotometer respectively, and completing data acquisition; 3, respectively carrying out maximum and minimum value normalization processing on the ultraviolet spectrum and the fluorescence spectrum to unify the data scale, and carrying out data preprocessing; step 4, screening key wavelengths by adopting a BOSS algorithm; step 5, optimizing the BP neural network by a PSO algorithm; and 6, after training is completed, evaluating indexes such as a decision coefficient, RMSE and a relative error, and after requirements are met, storing network parameters, and applying the network parameters to prediction of a to-be-tested sample. Compared with a single spectrum model, the precision of the detection method is improved by about 30%, and the detection accuracy of the trace pesticide in the complex water body is remarkably enhanced.

The invention provides a method for detecting mercury ions in wastewater, which comprises the following steps of: preparing a detection solution by taking a carbon quantum dot material as a detection reagent, mixing the detection solution with mercury ion solutions with different volumes to prepare a standard detection solution with gradient concentration, performing fluorescence test on the standard detection solution with gradient concentration, drawing a standard curve, and determining the mercury ions in the wastewater according to the standard curve. Mixing the detection solution with to-be-detected wastewater, detecting the absorbance of the to-be-detected wastewater, and calculating the concentration of mercury ions in the to-be-detected wastewater by using the standard curve. According to the method disclosed by the invention, detection can be completed by taking the quantum dots as an auxiliary detection reagent and adopting a fluorospectro photometer, so that the use of expensive detection equipment in inductively coupled plasma mass spectrometry and atomic fluorescence spectrometry is avoided, the detection cost can be effectively reduced, and the method also has the advantages of easiness in operation and high applicability.

The application provides a triphenylamine-benzopyrylium salt derivative NIR-BT-P and a synthesis method and application thereof.The Chinese name of the derivative NIR-BT-P is (E)-6-(diethylamino)-4-(4(diphenylamino)benzylidene)-1,2,3,4 tetrahydroxanthylium, and the English name is (E)-6-(diethylamino)-4-(4(diphenylamino)benzylidene)-1,2,3,4 tetrahydroxanthylium.The derivative NIR-BT-P is used as a fluorescent probe, different fluorescent intensity signals are achieved in different organic solvents through a fluorescence spectrophotometer, and the polarity is detected.The method utilizes the difference between cancer cells and normal cells in a living body, realizes near-infrared fluorescence imaging, and is used for distinguishing and detecting tumor tissues and normal tissues in the living body.

The invention discloses a sound field evaluation system and an evaluation method, and belongs to the field of ultrasonic transducer evaluation. A cuvette unit comprises a cuvette, and a macrophage suspension containing a sound-sensitive agent and an active oxygen probe is arranged in the cuvette; the ultrasonic unit comprises an ultrasonic transducer and an adjusting device, and the relative position of the ultrasonic transducer and the cuvette is adjusted through the adjusting device; and the evaluation unit is used for generating active oxygen in the macrophages and combining the active oxygen with the fluorescent probe under the ultrasonic action, exciting the active oxygen probe by the fluorospectro photometer, receiving an emitted light signal, obtaining a fluorescence intensity value, and outputting an evaluation result according to the fluorescence intensity value. According to the invention, two problems that desired experimental data cannot be obtained by single medicine and data measured by single engineering is long in time and complex in calculation are solved.

The utility model relates to the technical field of photometers, in particular to a fluorescence spectrophotometer. The device comprises a main body, a sample frame and a jacking assembly, a controller and a display screen are arranged on the front side of the device, a connecting plate and a fixing plate are arranged on the side face of the device, a sampling pipe is arranged at the bottom of the connecting plate and connected with a liquid pump in the main body, and the sample frame is arranged on the fixing plate. A jacking assembly aligned with the sampling tube is further arranged on the fixing plate, and the jacking assembly is provided with a jacking mechanism used for jacking and sampling a sample. According to the utility model, the rotatable sample frame is arranged, the sample changing mechanism is used for driving the sample frame to intermittently rotate at a fixed angle, so that samples can be aligned with the sampling tubes one by one without manually changing positions, meanwhile, the jacking assembly is arranged, and test tubes aligned with the sampling tubes in the sample frame can be driven by the jacking assembly to ascend; and the sampling tube is inserted into the test tube, so that sampling is realized, the whole operation is simple, and the sampling efficiency is high.

Faceted Benchtop Spectrofluorometer with Curved Front Panel

The invention relates to a self-assembled three-dimensional ball-flower type bimodal DNA sensor based on a rolling ring amplification technology, which is used for detecting target DNA of microorganisms in an early fouling stage. The sensor consists of a lock ring probe, target DNA (Deoxyribose Nucleic Acid) and a fluorescent nanogold probe. When the target DNA exists, the locked loop probe specifically recognizes the target DNA, rolling circle amplification is carried out under the action of T4DNA ligase and phi29 DNA polymerase, and a stable nano flower ball structure is formed through self-assembly. One end of the fluorescent signal probe modifies the fluorescent nanogold, and the other end of the fluorescent signal probe is complementary with a rolling circle amplification product to form a complete sensor after combination. The fluorescence spectrophotometer is used for measuring a fluorescence signal, the intensity of the fluorescence signal is positively correlated with the concentration of the target DNA, and qualitative and quantitative detection of the target DNA is realized. The nanometer flower ball structure enables a fluorescence signal to be enhanced and a color signal to be weakened, and self-calibration is achieved through comparison of the fluorescence signal and the color signal. The specific sequence of the sulfate reducing bacteria is taken as the target gene, so that the kit has high selectivity and anti-interference capability, and is suitable for rapid detection of early-stage fouling microorganisms.

The utility model discloses an atomic fluorescence spectrophotometer, and particularly relates to the technical field of atomic fluorescence spectrophotometers, which comprises an atomic fluorescence spectrophotometer, an atomizer is arranged in the atomic fluorescence spectrophotometer, a sampling cavity is arranged on the surface of the front side of the atomizer, and an adjusting mechanism is arranged in the sampling cavity. A protection mechanism is arranged on the front side of the atomizer; the adjusting mechanism comprises an upper moving plate and a lower moving plate, and the upper moving plate and the lower moving plate are both arranged in the sampling cavity. By arranging the protection mechanism and the flushing mechanism, corrosive chemical liquid can be effectively prevented from being splashed out through the arrangement of the protection door, the safety of operators is protected, after measurement is completed, water is pumped into the connecting water pipe and the telescopic hose through the water pump, a solution remaining in the microsyringe is flushed, and the safety of the microsyringe is improved. The influence of the last solution residue on the next detection precision is effectively prevented, and the accuracy of each detection is ensured.

This manufacturing inspection system measures an object to be inspected, which is a product, through fluorescent fingerprint measurement in a product manufacturing process and outputs a manufacturing inspection result. The manufacturing inspection system comprises: a spectrofluorometer that measures fluorescent fingerprint data of the object to be inspected; a fluorescent fingerprint data calculation unit that analyzes the measured fluorescent fingerprint data using a prescribed calculation method; a pass criterion storage unit that stores a pass criterion for a product specification formulated from at least one of a design specification range defined by design information of the product and a manufacturing specification range defined by manufacturing information; and a pass / fail determination unit that determines pass / fail by comparing a result of analysis by the fluorescent fingerprint data calculation unit with the pass criterion. This makes it possible to nondestructively measure the object to be inspected and automatically determine whether or not a predetermined criterion is satisfied.

The application discloses a method for realizing synchronous detection of mercury ions and lead ions based on a non-labeled DNA biosensor and a DNA probe thereof. The method has the advantages of sensitivity, simple operation and low cost. In the detection range, the concentrations of the two ions and the fluorescence signals are in good linear relationship. The application has good specificity for the two ions, and various metal ions such as potassium, copper, nickel, magnesium and calcium have no interference effect on the detection. The detection limits of the mercury ions and the lead ions are 4.094 nM and 3.22 nM respectively, and the whole detection process can be completed at room temperature. The result can be directly obtained through a fluorescence spectrophotometer. The test of the actual tap water sample has been completed, the detection process is convenient and fast, and the method can be used for detecting the mercury ions and the lead ions in actual water bodies.

The present invention proposes a method for diagnosing industrial wastewater mixing points based on fluorescence spectroscopy, specifically collecting water samples from inspection wells before and after possible mixing points, filtering with a filter membrane, detecting with an ultraviolet-visible spectrophotometer, and obtaining an EEM matrix by fluorescence spectrophotometer detection. The EEM matrix is ​​corrected for the fluorescence inner filter effect using ultraviolet-visible absorption spectrum data, and the primary and secondary Raman scattering and Rayleigh scattering signals are deducted from the EEM matrix. The EEM matrix is ​​peak-picked and numerically normalized. The EEM matrix of the inspection well water sample after the possible mixing point is subtracted from the EEM matrix before the possible mixing point to obtain a spectral residual matrix, which is compared with the spectral library to determine whether it is an actual mixing point, and the mixed water is traced. The present invention can better adapt to the characteristics of rainwater and sewage mixing in industrial areas, such as complex water quality, large differences in the position and intensity of fluorescence characteristic peaks, intermittent and sudden emission of pollution sources, etc., and improve the accuracy of diagnosis.