29 results about "Spectrofluorometer" patented technology

This invention discloses a method for determining fiber microplastics in aqueous textile solutions based on fluorescence spectrophotometry, belonging to the field of textile pollutant detection technology. The method includes: S1, collecting wastewater and filtering it through a filter membrane; S2, rinsing the filter membrane with sodium hypochlorite solution; S3, rinsing the fiber microplastics on the filter membrane into a separatory funnel; S4, pouring out a portion of the fiber microplastics from the upper end of the separatory funnel and collecting another portion from the lower end; S5, repeating S1-S4, rinsing the filter membrane with hydrochloric acid during this process, and collecting the remaining fiber microplastics; S6, dyeing the three solutions obtained; S7, preparing standard series solutions using polyester, polyamide, and polypropylene standard liner; S8, measuring and plotting a standard curve of fluorescence intensity versus fiber microplastic concentration using a fluorescence spectrophotometer; S9, measuring the fluorescence intensity of the three solutions obtained in S6. This invention is applicable to the field of aqueous textile solutions, enabling quantitative analysis of unknown samples, and can analyze both single and mixed samples with minimal detection bias.

The invention discloses a cyromazine concentration detection and analysis method and system, and relates to the technical field of pesticide residue detection.The method comprises the specific steps that a cyromazine detection chip is prepared, an integrated signal probe is prepared, a detection reaction system is constructed, triple cooperative response signals are collected, multiple signals are fused, and the cyromazine concentration is calculated; the cyromazine detection chip and the integrated signal probe are prepared, the fluorescence, electrochemistry and SERS triple synergistic response signal acquisition technology is combined, the accuracy of cyromazine concentration detection is improved, the gold nanoparticle modified substrate and the gold nanorod loaded fluorescence and electrochemical active substances are utilized, the response strength of the signal probe is enhanced, and the sensitivity of the signal probe is improved. The fluorescence spectrophotometer can detect cyromazine with lower concentration, meanwhile, the electrochemical workstation and the Raman spectrometer are introduced, so that the electrochemical peak current and SERS characteristic peak displacement are accurately acquired, signals from different sources are normalized, and errors existing among single signals are eliminated.

The invention discloses an activated sludge fluorescence online detection device based on a flow cell, and relates to the field of activated sludge biomass detection.The activated sludge fluorescence online detection device comprises a fluorescence spectrophotometer and a fluorescence spectrophotometer sample table arranged on the fluorescence spectrophotometer, and the fluorescence spectrophotometer is connected with a processor through a cable; the fluorescence spectrophotometer sample table is connected with a sample cup through a guide pipe, activated sludge is arranged in the sample cup, and a peristaltic pump is arranged on the guide pipe; the activated sludge continuously flows through the fluorescence spectrophotometer sample table through the guide pipe for three-dimensional fluorescence detection through the peristaltic pump, sludge sedimentation in a traditional cuvette is avoided, measurement is simple and convenient, and on-line real-time monitoring of the activated sludge biomass can be achieved.

The invention discloses a urease activity detection method based on a non-reversible reaction of dopamine and resorcinol. The urease activity detection method comprises the following steps: mixing a sample to be detected, a buffer solution with the pH value of 7.0 and a urea solution with a certain concentration; adding a certain amount of dopamine and resorcinol into the mixed solution, under the condition of 25-45 DEG C, catalyzing urea hydrolysis by urease, causing the pH value of the surrounding area of the urease to rise so as to form a local alkaline environment, further inducing the reaction of the dopamine and the resorcinol to generate a blue fluorescent substance, and adding excessive hydrochloric acid to terminate the reaction after the reaction is carried out for 40-120 minutes; and detecting the fluorescence intensity of the reaction liquid by using a fluorospectro photometer, and calculating the urease activity in the sample according to a fluorescence intensity-urease concentration standard curve. The urease activity detection method based on the reaction of dopamine and resorcinol has the advantages of high detection speed, high sensitivity, good selectivity and the like.

The present disclosure provides an integrating-sphere-assisted resonance synchronous (ISARS) spectroscopy method performed with an ISARS spectrophotometer, for example, a spectrofluorometer equipped with an integrating-sphere accessory. The methods and systems of the present disclosure utilize the ISARS spectrophotometer equipped with an integrating sphere to determine the ISARS spectral intensity, quantify the ISARS-based absorbance, and subsequently evaluate the sample's double-beam UV-vis absorbance spectrum (also known as the absorption extinction spectrum). This ISARS method enables quantitative separation of light absorption and scattering contribution to the sample UV-vis extinction spectrum measured with double-beam UV-vis spectrophotometer.

The utility model discloses a monitoring and compensating structure for eliminating the influence of EX spectrum fluctuation of a fluorospectro photometer, and particularly relates to the technical field of fluorescence analysis and detection, which comprises an EM light energy beam splitter, an EM wavelength energy matching tuner, a monitoring multiplier tube, an electronic negative feedback compensation control module and a measurement multiplier tube, the EM light energy beam splitter is arranged between a sample pool in the fluorescence spectrophotometer and the EM light energy beam splitter I, the EM light energy beam splitter splits EX light energy into two paths, the light beam I in the horizontal direction enters the fluorescence measurement channel to complete fluorescence testing, and the light beam II in the vertical direction enters the EX light energy monitoring channel to monitor the fluctuation of the EX light energy. According to the utility model, a monitoring light path structure of which the photoelectric characteristic of a monitoring light path is consistent with that of a measuring light path detector is designed to be embedded into a measuring light path and a circuit of an instrument, so that the correction compensation quantity of fluctuation quantity which is equal to sample fluorescence in quantity ratio and same in phase and synchronization is obtained in time to eliminate the error influence caused by EX energy fluctuation in a fluorescence test.

The invention discloses a preparation method and device of an intelligent biosensor. The preparation method comprises the following steps: preparing mixed solutions with different concentrations, transferring the mixed solutions to a cuvette of a fluorospectro photometer, and carrying out fluorescence intensity analysis on the mixed solutions in a wavelength range of 350-500 nm; establishing a linear relation of maximum fluorescence emission peak values corresponding to different concentrations; establishing a linear relation between chromatic values corresponding to different concentrations; photographing and extracting a chromatic value by using an RGB algorithm; and comparing the linear relationship to obtain the corresponding concentration. Important data support is provided for tumor diagnosis, chemotherapy drug resistance analysis and personalized treatment. The method combines an intelligent sensing technology and a portable platform, is suitable for intelligent medical equipment and cancer biomarker detection, and has a wide application prospect.

The invention discloses a DNA (Deoxyribose Nucleic Acid) probe ATP-3 and application thereof. The DNA (Deoxyribose Nucleic Acid) probe ATP-3 disclosed by the invention comprises DOX-3 and DOX-3-CP (Chlorinated Drug detection can be realized by using the DNA probe ATP-3 based on a non-label method DNA biosensor. Based on a double-strand competitive binding mechanism (DOX-3 and DOX specific binding leads to double-strand dissociation) of a DNA probe ATP-3, and in combination with a fluorescence switching characteristic (fluorescence enhancement during double-strand binding and quenching during dissociation) of an embedded fluorescent agent DAPI, trace detection of drugs is realized. By using a fluorospectro photometer as a detection auxiliary instrument, the method has the advantages of sensitive result, simplicity in operation, lower cost and the like.

This utility model discloses a fluorescence performance testing device for fluorescent composite fibers, relating to the field of composite fiber processing technology. The device includes a fluorescence spectrophotometer. A cover plate is connected to one side of the top of the spectrophotometer via a damping shaft. A first light-shielding strip, a second light-shielding strip, and two curved grooves are respectively provided on both sides of the cover plate. Both the first and second light-shielding strips have curved grooves and curved openings on their sides near the cover plate. A first sealing strip is connected to the bottom of the first light-shielding strip. Through the arrangement of the cover plate, adsorption block, electromagnet, first light-shielding strip, and first sealing strip, the device allows the first and second light-shielding strips to rotate during the opening and closing of the cover plate. The increased width of the cover plate allows it to directly rest on the top and surface of the fluorescence spectrophotometer, rather than being embedded inside, thus avoiding structural wear. This effectively prevents structural wear and light leakage caused by an improperly closed cover plate.

The invention belongs to the technical field of pesticide residue detection, and particularly discloses an ultraviolet-fluorescence spectrum fused cyromazine detection method based on a BOSS-PSO-BP neural network, and the method comprises the following steps: step 1, preparing a cyromazine sample with gradient concentration of 2.25 * 10 <-6 >-1.47 * 10 <-4 > mg / mL for training a neural network; 2, detecting the samples with different concentrations by using an LS55 fluorescence spectrophotometer and a UV-6300 ultraviolet spectrophotometer respectively, and completing data acquisition; 3, respectively carrying out maximum and minimum value normalization processing on the ultraviolet spectrum and the fluorescence spectrum to unify the data scale, and carrying out data preprocessing; step 4, screening key wavelengths by adopting a BOSS algorithm; step 5, optimizing the BP neural network by a PSO algorithm; and 6, after training is completed, evaluating indexes such as a decision coefficient, RMSE and a relative error, and after requirements are met, storing network parameters, and applying the network parameters to prediction of a to-be-tested sample. Compared with a single spectrum model, the precision of the detection method is improved by about 30%, and the detection accuracy of the trace pesticide in the complex water body is remarkably enhanced.

The utility model discloses an atomic fluorescence spectrophotometer, and particularly relates to the technical field of atomic fluorescence spectrophotometers, which comprises an atomic fluorescence spectrophotometer, an atomizer is arranged in the atomic fluorescence spectrophotometer, a sampling cavity is arranged on the surface of the front side of the atomizer, and an adjusting mechanism is arranged in the sampling cavity. A protection mechanism is arranged on the front side of the atomizer; the adjusting mechanism comprises an upper moving plate and a lower moving plate, and the upper moving plate and the lower moving plate are both arranged in the sampling cavity. By arranging the protection mechanism and the flushing mechanism, corrosive chemical liquid can be effectively prevented from being splashed out through the arrangement of the protection door, the safety of operators is protected, after measurement is completed, water is pumped into the connecting water pipe and the telescopic hose through the water pump, a solution remaining in the microsyringe is flushed, and the safety of the microsyringe is improved. The influence of the last solution residue on the next detection precision is effectively prevented, and the accuracy of each detection is ensured.

This manufacturing inspection system measures an object to be inspected, which is a product, through fluorescent fingerprint measurement in a product manufacturing process and outputs a manufacturing inspection result. The manufacturing inspection system comprises: a spectrofluorometer that measures fluorescent fingerprint data of the object to be inspected; a fluorescent fingerprint data calculation unit that analyzes the measured fluorescent fingerprint data using a prescribed calculation method; a pass criterion storage unit that stores a pass criterion for a product specification formulated from at least one of a design specification range defined by design information of the product and a manufacturing specification range defined by manufacturing information; and a pass / fail determination unit that determines pass / fail by comparing a result of analysis by the fluorescent fingerprint data calculation unit with the pass criterion. This makes it possible to nondestructively measure the object to be inspected and automatically determine whether or not a predetermined criterion is satisfied.

The application discloses a method for realizing synchronous detection of mercury ions and lead ions based on a non-labeled DNA biosensor and a DNA probe thereof. The method has the advantages of sensitivity, simple operation and low cost. In the detection range, the concentrations of the two ions and the fluorescence signals are in good linear relationship. The application has good specificity for the two ions, and various metal ions such as potassium, copper, nickel, magnesium and calcium have no interference effect on the detection. The detection limits of the mercury ions and the lead ions are 4.094 nM and 3.22 nM respectively, and the whole detection process can be completed at room temperature. The result can be directly obtained through a fluorescence spectrophotometer. The test of the actual tap water sample has been completed, the detection process is convenient and fast, and the method can be used for detecting the mercury ions and the lead ions in actual water bodies.

The present application belongs to the technical field of aluminum salt determination of injection products in the pharmaceutical industry, and particularly relates to a method for determining aluminum salt in peritoneal dialysis fluid by using a fluorescence spectrophotometer. 3+ The complex formed in ethyl acetate has good stability and strong fluorescence intensity, and the sample is treated with concentrated hydrochloric acid and extracted with 1,1-dimethylpropanone (MIPK for short), so that the interference of transition metal impurity ions is eliminated. At the same time, the special determination parameters are specified, and the method after the research is verified well, and meets the aluminum salt inspection of peritoneal dialysis fluid products.

To provide an agent which does not utilize the toxicity of metal ions and exhibits one or two or more of high antibacterial, antifungal and antiviral properties in spite of a light color tone.SOLUTION: An agent comprising La (OH) 3, La2O2CO3, and ZnO, wherein a ratio (I (384) / I (560)) of a fluorescence emission intensity (I (560)) at a wave length of 365nm to a fluorescence emission intensity (I (384)) at a wave length of 560nm is 0.40 or more when the agent is irradiated with light having a wave length of 384nm and subjected to spectrofluorometric analysis, and the agent is selected from antibacterial agents, antifungal agents, and antiviral agents.SELECTED DRAWING: None

The invention discloses a fluorescence detection method for exosome drug loading efficiency based on low-speed centrifugal separation. The method comprises the following steps: separating an exosome from a biological sample through low-speed centrifugation; labeling the drug with a fluorescent dye, and carrying the drug through a co-incubation or ultrasonic method; purifying the drug-loaded exosome by adopting low-speed centrifugation; and measuring the fluorescence intensity by using a fluorospectro photometer, and calculating the drug loading efficiency through a standard curve. According to the method, equipment is simple, operation is convenient, and rapid and low-cost detection can be achieved; by adopting low-speed centrifugal separation of the exosome and combining a fluorescence labeling technology, the equipment dependence and the operation complexity are reduced, so that a conventional laboratory without ultracentrifugation and liquid chromatography equipment can also efficiently detect the drug loading efficiency of the exosome, thereby solving the problems of high equipment requirement and complicated operation in the prior art, and improving the detection efficiency of the exosome. Meanwhile, the method is simple in step and easy to implement, and professional training requirements are reduced.

The invention provides a method for detecting aflatoxin B1 based on a magnetic immunofluorescence probe, which comprises the following steps: according to the specific recognition principle of an AFB1 antibody to AFB1, competitively recognizing AFB1 and a magnetic microballe-AFB1 conjugate in a sample through a QSH630-antibody conjugate, separating the QSH630-anti-AFB1-magnetic microsphere conjugate by using an external magnetic field, and detecting aflatoxin B1 by using a magnetic immunofluorescence probe. The fluorescence intensity of the red quantum dots in the system is reduced. The fluorescence intensity of the remaining red quantum dots is detected through a fluorospectro photometer, a linear standard curve of the fluorescence intensity and the concentration of the AFB1 is obtained, the concentration of the AFB1 and the fluorescence intensity are in a good linear correlation within the range of 1.25-4.75 pg / mL, and therefore high-sensitivity detection of the AFB1 is achieved. The invention establishes a simple, convenient, rapid and sensitive AFB1 specific analysis method, and has important significance on prevention and control of AFB1 pollution.

The present application provides a method for measuring zinc in a solution, the method comprising: preparing an indicator solution wherein the indicator solution comprises 8-hydroxy-5-quinolinesulfonic acid; introducing an indicator solution into the solution wherein the solution contains an amount of zinc and the introduction causes a fluorescence change in the solution in response to the indicator solution reacting with zinc; adding a moderator to the solution, wherein the moderator prevents the interferent from binding to the indicator; and measuring the amount of zinc in the solution by measuring a change in fluorescence intensity and comparing the change in intensity to a calibration curve generated based on conditions of the solution, where the conditions affect the fluorescence measurement of the solution, where the fluorescence intensity is proportional to the concentration of zinc in the solution, wherein low-range zinc detection is used, and the low-range zinc detection uses an emission scanning mode of a fluorospectro photometer. Other aspects are described and claimed.

The invention discloses a biological activity detection method and device based on deep learning, and relates to the technical field of biological detection.The biological activity detection method comprises the steps that firstly, a conjugated polymer probe containing triphenylamine and methylimidazole active units is prepared through Suzuki coupling reaction, and the aggregation-induced quenching effect is inhibited; carrying out standardized centrifugal impurity removal and dilution constant volume treatment on the sample, and collecting spectrum and RGB signals by combining a fluorospectro photometer and a portable device; and finally, realizing accurate detection and chain length distinguishing of the low-concentration pollutants by utilizing linear fitting and linear discriminant analysis algorithms. The device comprises a detection table, a multifunctional linkage mechanism and a self-adjusting protection mechanism are integrated, and accurate sample positioning, elastic buffering clamping and multi-angle posture adjustment are achieved through meshing transmission. The problems of low sensitivity, poor specificity, insufficient device suitability and the like of traditional detection are solved, the detection reliability and the scene suitability are improved, and the method is suitable for the fields of environment monitoring, food detection and the like.

This invention discloses a rapid detection method for lomefloxacin based on fluorescent carbon dots. The method applies fluorescent carbon dots to the detection of lomefloxacin drug concentration and includes the following steps: (1) preparing a carbon dot solution by mixing fluorescent carbon dots and water, then adding lomefloxacin standard solutions of different concentrations, using 395nm as the excitation wavelength, and measuring the emission intensity at 485nm wavelength using a fluorescence spectrophotometer to obtain a standard curve equation; (2) calculating the fluorescence intensity change value of the sample to be tested according to y = F - F0, and then substituting the measured fluorescence intensity change value of the sample to be tested into the standard curve to calculate the concentration of lomefloxacin to be tested. This invention provides a new pathway for the detection of lomefloxacin, with advantages such as convenient construction, high sensitivity, good specificity, and ease of use. It can be applied to the determination of drug concentration in lomefloxacin raw materials and their preparations, as well as in veterinary drug residues.

The application discloses a kind of in-situ measurement methods of aquatic primary productivity based on fluorescence excitation spectrum three-wavelength fitting model, belong to environmental monitoring and aquatic ecosystem technical field.The application includes: in-situ collection target water body water sample and filter to remove large particle impurities;Water sample is moved into fluorescence spectrophotometer sample cell, and after dark chamber balancing, the fluorescence intensity of 438nm, 485nm, 624nm three characteristic wavelengths is measured;According to water sample type, the integral area of fluorescence excitation spectrum is calculated using corresponding first-order linear fitting equation;Through the correlation model of integral area and primary productivity, the primary productivity of target water body is calculated.The application does not need sample pretreatment, simple and fast, measurement period is ≤30min, realizes in-situ non-invasive monitoring;Cover a variety of photosynthetic pigment characteristic absorption peak, wide application range, can tolerate a variety of stress conditions;Measurement precision is high, sensitivity is strong.

The application discloses a method for detecting sodium formaldehyde sulfoxylate based on nitrogen-doped graphene quantum dots ratio fluorescence, and belongs to the technical field of harmful compound detection in food. The method is characterized in that: a to-be-detected sample solution, a nitrogen-doped graphene quantum dots solution and a 1,4-naphthoquinone solution are mixed to react, after the reaction, ratio fluorescence response (I 420 / I 440 ) under an excitation wavelength of 360 nm is measured by a fluorescence spectrophotometer, the measured fluorescence intensity signal is introduced into a standard curve, and the concentration of sodium formaldehyde sulfoxylate in the to-be-detected sample is calculated and obtained. The application has the advantages of low cost, simple operation, visible detection result, no need of using large instruments and professional operators, and accurate and reliable detection result, and can be used for qualitative and quantitative detection of ivory white.

The invention relates to the technical field of drug screening, in particular to a method for screening G-quadruplex small molecule drug ligands. The method comprises the following steps: S1, preparing a Cu-TCPP / rGO nano composite solution; s2, the Cu-TCPP / rGO nano-composite solution is mixed with a fluorescein-labeled G-base-rich deoxyribonucleic acid solution, and a buffer solution is added to dilute the Cu-TCPP / rGO nano-composite solution and the G-base-rich deoxyribonucleic acid solution; s3, adding traditional Chinese medicine monomers with different concentrations into the mixed solution obtained in the step S2, and determining the fluorescence intensity of the mixed solution by using a fluorospectro photometer; and S4, screening the G-quadruplex small molecule drug ligand according to the fluorescence intensity of the mixed solution. According to the method for screening the G-quadruplex small molecule drug ligand, the application range of the 2D MOFs nano material can be expanded, and reference can be provided for screening of anti-tumor drugs.

The invention discloses a salmonella typhimurium fluorescence detection method based on dual signal amplification, and relates to the technical field of microbiological detection.The method comprises the specific steps that firstly, SNA1 and SNA2 probes are constructed, related chains are co-modified on the surfaces of gold nanoparticles through a freezing method, and part of sequences are closed; then preparing an Apt.-F compound; then incubating the sample and the compound; then starting a dual signal amplification reaction; and finally, detecting the fluorescence intensity by using a fluorospectro photometer, and quantifying the salmonella typhimurium according to the relationship between the fluorescence intensity and the bacterium concentration. According to the invention, two kinds of functionalized SNA probes are constructed, the aptamer and DNAzyme are arranged, a dual signal amplification pathway is established, target bacteria are accurately identified through the aptamer, a trigger chain is released to start chain replacement, a fluorophore is released, the detection sensitivity is improved, the probe design is optimized to ensure specificity, the process is simplified, and rapid quantification is realized; an efficient scheme is provided for trace pathogenic bacterium detection.

The invention relates to a method for measuring the filtering efficiency of a nanoscale filter material and a filter element, which comprises the following steps: preparing standard solutions with different fluorescent microsphere concentrations, the standard solutions comprising fluorescent microspheres and deionized water; measuring the fluorescence value of each standard solution by using a fluorospectro photometer to obtain a standard curve of different fluorescent microsphere concentrations and fluorescence values; preparing a test solution, wherein the test solution comprises fluorescent microspheres and deionized water; enabling the prepared test liquid to pass through the filter element to be tested by adopting a single pass method; measuring fluorescence values of the test liquid at an inlet and an outlet of the filter element; determining the fluorescent microsphere concentration of the test liquid at the inlet and the outlet according to the standard curve and the fluorescence values of the test liquid at the inlet and the outlet; and calculating the filtering efficiency according to a formula. According to the method, the requirement for cleanliness is low, the test efficiency is greatly improved, the cleaning time is shortened, and the cleaning cost is reduced; the test and maintenance cost of equipment and the requirement on the detection environment are obviously reduced, and test conditions are provided for the development of high-precision filter materials and filter elements.

This invention provides a quantitative method for microplastics in water, specifically utilizing the fluorescence properties of the benzene ring in polystyrene (PS) to quantify polystyrene microplastics in water, belonging to the field of environmental monitoring. The steps are as follows: determining the position of the fluorescence peak of polystyrene microplastics; preparing a standard polystyrene microplastic stock solution; measuring the fluorescence intensity of the suspension using a fluorescence spectrophotometer; plotting a standard curve of polystyrene microplastic fluorescence intensity versus mass concentration; and calculating the mass concentration of microplastics based on the fluorescence intensity of the actual water sample and the standard curve. This method can accurately quantify the concentration of polystyrene microplastics in water samples.

The invention discloses a method for detecting aflatoxin B1 in rice based on an up-conversion-gold-coated-silicon fluorescence quenching recovery mode, and belongs to the technical field of food safety detection. The detection method specifically comprises the following steps: taking a sample extracting solution to react with a UCNPs-Apt fluorescent probe, then adding a Si-coated AuNPs-cDNA quenching probe to continuously react, determining the fluorescence intensity by adopting a fluorospectro photometer after the reaction, and calculating the content of AFB1 in a sample to be detected according to a standard curve, according to the method, an up-conversion-gold-coated-silicon fluorescence quenching recovery mode is adopted, complex sample pretreatment and long-time separation and purification steps are not needed, and detection can be completed through simple mixed reaction and excitation determination; on the premise of ensuring the detection sensitivity and accuracy, the detection efficiency is improved, and the quick response requirement of the grain circulation link is met.

The utility model relates to the technical field of water detection, in particular to a pretreatment device for detecting a water sample by using a fluorospectro photometer, which comprises a base, a controller, a filtering mechanism, a diluting mechanism and a treatment mechanism, the diluting mechanism comprises a water storage tank, a liquid metering pump and a shunting assembly; the filtering mechanism comprises a plurality of filter membranes and a plurality of sample adding assemblies. According to the pretreatment device for detecting the water sample by the fluorospectro photometer, the water sample to be detected can be automatically filtered and diluted, so that the subsequent detection standard is met, manual filtering and filtering operation are not needed, a large number of steps are reduced, the pretreatment time is saved, and the detection efficiency is improved. The pretreatment efficiency of the water sample is improved, and the detection accuracy is improved.

The invention discloses a method for detecting total polar substances of edible oil based on a trithiophene type fluorescent probe, which comprises the following steps: dissolving the trithiophene type fluorescent probe in 1, 4-dioxane to prepare a probe solution; the method comprises the following steps: adding a probe solution dissolved in 1, 4-dioxane into edible oil containing different contents of total polar substances, recording fluorescence intensity through a fluorospectro photometer, and constructing a linear standard curve through a linear relation between the content of the total polar substances and the fluorescence intensity; and adding the probe solution into the edible oil, recording the change of the fluorescence intensity, and substituting the change into the standard curve to obtain the total polar substance content of the edible oil. The probe (Z)-3-(5 '-(4-(bis (4-methoxyphenyl) amino) phenyl)-[2, 2': 5 ', 2'-terthienyl]-5-yl)-2-(5-(trifluoromethyl) pyridine-2-yl) acrylonitrile selectively detects the polarity of the edible oil, red fluorescence is quenched after the polarity of the edible oil is increased, and the probe can be used as a fluorescent probe for detecting the polarity of the edible oil.