39 results about "Spectrofluorometer" patented technology

This invention discloses a method for determining fiber microplastics in aqueous textile solutions based on fluorescence spectrophotometry, belonging to the field of textile pollutant detection technology. The method includes: S1, collecting wastewater and filtering it through a filter membrane; S2, rinsing the filter membrane with sodium hypochlorite solution; S3, rinsing the fiber microplastics on the filter membrane into a separatory funnel; S4, pouring out a portion of the fiber microplastics from the upper end of the separatory funnel and collecting another portion from the lower end; S5, repeating S1-S4, rinsing the filter membrane with hydrochloric acid during this process, and collecting the remaining fiber microplastics; S6, dyeing the three solutions obtained; S7, preparing standard series solutions using polyester, polyamide, and polypropylene standard liner; S8, measuring and plotting a standard curve of fluorescence intensity versus fiber microplastic concentration using a fluorescence spectrophotometer; S9, measuring the fluorescence intensity of the three solutions obtained in S6. This invention is applicable to the field of aqueous textile solutions, enabling quantitative analysis of unknown samples, and can analyze both single and mixed samples with minimal detection bias.

The invention discloses a cyromazine concentration detection and analysis method and system, and relates to the technical field of pesticide residue detection.The method comprises the specific steps that a cyromazine detection chip is prepared, an integrated signal probe is prepared, a detection reaction system is constructed, triple cooperative response signals are collected, multiple signals are fused, and the cyromazine concentration is calculated; the cyromazine detection chip and the integrated signal probe are prepared, the fluorescence, electrochemistry and SERS triple synergistic response signal acquisition technology is combined, the accuracy of cyromazine concentration detection is improved, the gold nanoparticle modified substrate and the gold nanorod loaded fluorescence and electrochemical active substances are utilized, the response strength of the signal probe is enhanced, and the sensitivity of the signal probe is improved. The fluorescence spectrophotometer can detect cyromazine with lower concentration, meanwhile, the electrochemical workstation and the Raman spectrometer are introduced, so that the electrochemical peak current and SERS characteristic peak displacement are accurately acquired, signals from different sources are normalized, and errors existing among single signals are eliminated.

The invention discloses an activated sludge fluorescence online detection device based on a flow cell, and relates to the field of activated sludge biomass detection.The activated sludge fluorescence online detection device comprises a fluorescence spectrophotometer and a fluorescence spectrophotometer sample table arranged on the fluorescence spectrophotometer, and the fluorescence spectrophotometer is connected with a processor through a cable; the fluorescence spectrophotometer sample table is connected with a sample cup through a guide pipe, activated sludge is arranged in the sample cup, and a peristaltic pump is arranged on the guide pipe; the activated sludge continuously flows through the fluorescence spectrophotometer sample table through the guide pipe for three-dimensional fluorescence detection through the peristaltic pump, sludge sedimentation in a traditional cuvette is avoided, measurement is simple and convenient, and on-line real-time monitoring of the activated sludge biomass can be achieved.

The invention belongs to the technical field of biology, and particularly relates to regulation and control of cinnamyl aldehyde on hyperuricemia related gene promoter region G quadruplex. According to the application disclosed by the invention, firstly, the capacity of cinnamyl aldehyde for regulating and controlling a G quadruplex in a hyperuricemia related gene promoter region is explored by utilizing circular dichroism, and the influence of cinnamyl aldehyde on regulation and control of a nucleic acid structure is further evaluated by utilizing a fluorospectro photometer, so that support is provided for a molecular mechanism of cinnamyl aldehyde for relieving hyperuricemia on a gene level. In conclusion, the invention discloses a brand new mechanism that cinnamyl aldehyde regulates and controls uric acid metabolism related genes through a targeted G quadruplex structure, a precise action target and a potential drug development direction are provided for treatment of hyperuricemia, and meanwhile, an established small molecule and G quadruplex interaction verification method provides technical reference for similar research.

The invention discloses a urease activity detection method based on a non-reversible reaction of dopamine and resorcinol. The urease activity detection method comprises the following steps: mixing a sample to be detected, a buffer solution with the pH value of 7.0 and a urea solution with a certain concentration; adding a certain amount of dopamine and resorcinol into the mixed solution, under the condition of 25-45 DEG C, catalyzing urea hydrolysis by urease, causing the pH value of the surrounding area of the urease to rise so as to form a local alkaline environment, further inducing the reaction of the dopamine and the resorcinol to generate a blue fluorescent substance, and adding excessive hydrochloric acid to terminate the reaction after the reaction is carried out for 40-120 minutes; and detecting the fluorescence intensity of the reaction liquid by using a fluorospectro photometer, and calculating the urease activity in the sample according to a fluorescence intensity-urease concentration standard curve. The urease activity detection method based on the reaction of dopamine and resorcinol has the advantages of high detection speed, high sensitivity, good selectivity and the like.

The present disclosure provides an integrating-sphere-assisted resonance synchronous (ISARS) spectroscopy method performed with an ISARS spectrophotometer, for example, a spectrofluorometer equipped with an integrating-sphere accessory. The methods and systems of the present disclosure utilize the ISARS spectrophotometer equipped with an integrating sphere to determine the ISARS spectral intensity, quantify the ISARS-based absorbance, and subsequently evaluate the sample's double-beam UV-vis absorbance spectrum (also known as the absorption extinction spectrum). This ISARS method enables quantitative separation of light absorption and scattering contribution to the sample UV-vis extinction spectrum measured with double-beam UV-vis spectrophotometer.

The invention provides a single-molecule fluorescent probe based on a BODIPY derivative BP-BNO2 as well as a synthesis method and application of the single-molecule fluorescent probe, and the specific Chinese name of the derivative is 5, 5-difluoro-1, 3, 7, 9-tetramethyl-10-(4-nitrophenyl)-5H-4 lambda 4, 5 lambda 4-dipyrrolo [1, 2-c: 2 ', 1'-f] [1, 3, 2] diazaborane. The invention also provides a synthesis method of the derivative and application of the derivative in detection of hypobromous acid in vitro, cells and nasal cavities of mice with allergic rhinitis. According to the method, on the basis of a derivative BP-BNO2 of BODIPY, the concentration change of hypobromous acid is detected through a fluorospectro photometer in a mixed solution of a phosphate buffer solution and acetonitrile (the volume ratio is 7: 3). The method also realizes noninvasive in-situ imaging at cell and mouse levels, and can be used for in-situ monitoring of hypobromous acid level in the nasal cavity of the mouse with allergic rhinitis and auxiliary diagnosis of allergic rhinitis.

The utility model discloses a monitoring and compensating structure for eliminating the influence of EX spectrum fluctuation of a fluorospectro photometer, and particularly relates to the technical field of fluorescence analysis and detection, which comprises an EM light energy beam splitter, an EM wavelength energy matching tuner, a monitoring multiplier tube, an electronic negative feedback compensation control module and a measurement multiplier tube, the EM light energy beam splitter is arranged between a sample pool in the fluorescence spectrophotometer and the EM light energy beam splitter I, the EM light energy beam splitter splits EX light energy into two paths, the light beam I in the horizontal direction enters the fluorescence measurement channel to complete fluorescence testing, and the light beam II in the vertical direction enters the EX light energy monitoring channel to monitor the fluctuation of the EX light energy. According to the utility model, a monitoring light path structure of which the photoelectric characteristic of a monitoring light path is consistent with that of a measuring light path detector is designed to be embedded into a measuring light path and a circuit of an instrument, so that the correction compensation quantity of fluctuation quantity which is equal to sample fluorescence in quantity ratio and same in phase and synchronization is obtained in time to eliminate the error influence caused by EX energy fluctuation in a fluorescence test.

The invention discloses a preparation method and device of an intelligent biosensor. The preparation method comprises the following steps: preparing mixed solutions with different concentrations, transferring the mixed solutions to a cuvette of a fluorospectro photometer, and carrying out fluorescence intensity analysis on the mixed solutions in a wavelength range of 350-500 nm; establishing a linear relation of maximum fluorescence emission peak values corresponding to different concentrations; establishing a linear relation between chromatic values corresponding to different concentrations; photographing and extracting a chromatic value by using an RGB algorithm; and comparing the linear relationship to obtain the corresponding concentration. Important data support is provided for tumor diagnosis, chemotherapy drug resistance analysis and personalized treatment. The method combines an intelligent sensing technology and a portable platform, is suitable for intelligent medical equipment and cancer biomarker detection, and has a wide application prospect.

The invention discloses a DNA (Deoxyribose Nucleic Acid) probe ATP-3 and application thereof. The DNA (Deoxyribose Nucleic Acid) probe ATP-3 disclosed by the invention comprises DOX-3 and DOX-3-CP (Chlorinated Drug detection can be realized by using the DNA probe ATP-3 based on a non-label method DNA biosensor. Based on a double-strand competitive binding mechanism (DOX-3 and DOX specific binding leads to double-strand dissociation) of a DNA probe ATP-3, and in combination with a fluorescence switching characteristic (fluorescence enhancement during double-strand binding and quenching during dissociation) of an embedded fluorescent agent DAPI, trace detection of drugs is realized. By using a fluorospectro photometer as a detection auxiliary instrument, the method has the advantages of sensitive result, simplicity in operation, lower cost and the like.

The utility model relates to the technical field of automatic sample injectors, in particular to an automatic sample injector of an atomic fluorescence spectrophotometer, which comprises a box body, a transverse plate is fixedly connected below the inner wall of the box body, a controller is fixedly connected below one side of the box body, a shell is fixedly connected at the top of the box body, and the transverse plate is fixedly connected with the shell. A material pipe is fixedly connected to the inner wall of one side of the top of the shell, the inner wall of the bottom of the material pipe is fixedly connected with a water inlet pipe of the conveying pump, and a valve is fixedly connected to the inner wall of the lower portion of one side of the material pipe. Through cooperation of a box body, a transverse plate and a fixing structure, when the device is used, a sample tube can be placed between two clamping plates, a lead screw is rotated, the lead screw drives a sliding block to move, the sliding block drives the clamping plate on one side to move, then the two clamping plates are made to be close to each other, a rubber block is attached to the sample tube, and then the sample tube is fixed; the sample bottles with different sizes can be conveniently fixed, so that the sample injection work can be conveniently carried out on the sample bottles with different sizes.

This utility model discloses a fluorescence performance testing device for fluorescent composite fibers, relating to the field of composite fiber processing technology. The device includes a fluorescence spectrophotometer. A cover plate is connected to one side of the top of the spectrophotometer via a damping shaft. A first light-shielding strip, a second light-shielding strip, and two curved grooves are respectively provided on both sides of the cover plate. Both the first and second light-shielding strips have curved grooves and curved openings on their sides near the cover plate. A first sealing strip is connected to the bottom of the first light-shielding strip. Through the arrangement of the cover plate, adsorption block, electromagnet, first light-shielding strip, and first sealing strip, the device allows the first and second light-shielding strips to rotate during the opening and closing of the cover plate. The increased width of the cover plate allows it to directly rest on the top and surface of the fluorescence spectrophotometer, rather than being embedded inside, thus avoiding structural wear. This effectively prevents structural wear and light leakage caused by an improperly closed cover plate.

The invention belongs to the technical field of pesticide residue detection, and particularly discloses an ultraviolet-fluorescence spectrum fused cyromazine detection method based on a BOSS-PSO-BP neural network, and the method comprises the following steps: step 1, preparing a cyromazine sample with gradient concentration of 2.25 * 10 <-6 >-1.47 * 10 <-4 > mg / mL for training a neural network; 2, detecting the samples with different concentrations by using an LS55 fluorescence spectrophotometer and a UV-6300 ultraviolet spectrophotometer respectively, and completing data acquisition; 3, respectively carrying out maximum and minimum value normalization processing on the ultraviolet spectrum and the fluorescence spectrum to unify the data scale, and carrying out data preprocessing; step 4, screening key wavelengths by adopting a BOSS algorithm; step 5, optimizing the BP neural network by a PSO algorithm; and 6, after training is completed, evaluating indexes such as a decision coefficient, RMSE and a relative error, and after requirements are met, storing network parameters, and applying the network parameters to prediction of a to-be-tested sample. Compared with a single spectrum model, the precision of the detection method is improved by about 30%, and the detection accuracy of the trace pesticide in the complex water body is remarkably enhanced.

The invention provides a method for detecting mercury ions in wastewater, which comprises the following steps of: preparing a detection solution by taking a carbon quantum dot material as a detection reagent, mixing the detection solution with mercury ion solutions with different volumes to prepare a standard detection solution with gradient concentration, performing fluorescence test on the standard detection solution with gradient concentration, drawing a standard curve, and determining the mercury ions in the wastewater according to the standard curve. Mixing the detection solution with to-be-detected wastewater, detecting the absorbance of the to-be-detected wastewater, and calculating the concentration of mercury ions in the to-be-detected wastewater by using the standard curve. According to the method disclosed by the invention, detection can be completed by taking the quantum dots as an auxiliary detection reagent and adopting a fluorospectro photometer, so that the use of expensive detection equipment in inductively coupled plasma mass spectrometry and atomic fluorescence spectrometry is avoided, the detection cost can be effectively reduced, and the method also has the advantages of easiness in operation and high applicability.

The application provides a triphenylamine-benzopyrylium salt derivative NIR-BT-P and a synthesis method and application thereof.The Chinese name of the derivative NIR-BT-P is (E)-6-(diethylamino)-4-(4(diphenylamino)benzylidene)-1,2,3,4 tetrahydroxanthylium, and the English name is (E)-6-(diethylamino)-4-(4(diphenylamino)benzylidene)-1,2,3,4 tetrahydroxanthylium.The derivative NIR-BT-P is used as a fluorescent probe, different fluorescent intensity signals are achieved in different organic solvents through a fluorescence spectrophotometer, and the polarity is detected.The method utilizes the difference between cancer cells and normal cells in a living body, realizes near-infrared fluorescence imaging, and is used for distinguishing and detecting tumor tissues and normal tissues in the living body.

Faceted Benchtop Spectrofluorometer with Curved Front Panel

The utility model discloses an atomic fluorescence spectrophotometer, and particularly relates to the technical field of atomic fluorescence spectrophotometers, which comprises an atomic fluorescence spectrophotometer, an atomizer is arranged in the atomic fluorescence spectrophotometer, a sampling cavity is arranged on the surface of the front side of the atomizer, and an adjusting mechanism is arranged in the sampling cavity. A protection mechanism is arranged on the front side of the atomizer; the adjusting mechanism comprises an upper moving plate and a lower moving plate, and the upper moving plate and the lower moving plate are both arranged in the sampling cavity. By arranging the protection mechanism and the flushing mechanism, corrosive chemical liquid can be effectively prevented from being splashed out through the arrangement of the protection door, the safety of operators is protected, after measurement is completed, water is pumped into the connecting water pipe and the telescopic hose through the water pump, a solution remaining in the microsyringe is flushed, and the safety of the microsyringe is improved. The influence of the last solution residue on the next detection precision is effectively prevented, and the accuracy of each detection is ensured.

This manufacturing inspection system measures an object to be inspected, which is a product, through fluorescent fingerprint measurement in a product manufacturing process and outputs a manufacturing inspection result. The manufacturing inspection system comprises: a spectrofluorometer that measures fluorescent fingerprint data of the object to be inspected; a fluorescent fingerprint data calculation unit that analyzes the measured fluorescent fingerprint data using a prescribed calculation method; a pass criterion storage unit that stores a pass criterion for a product specification formulated from at least one of a design specification range defined by design information of the product and a manufacturing specification range defined by manufacturing information; and a pass / fail determination unit that determines pass / fail by comparing a result of analysis by the fluorescent fingerprint data calculation unit with the pass criterion. This makes it possible to nondestructively measure the object to be inspected and automatically determine whether or not a predetermined criterion is satisfied.

The application discloses a method for realizing synchronous detection of mercury ions and lead ions based on a non-labeled DNA biosensor and a DNA probe thereof. The method has the advantages of sensitivity, simple operation and low cost. In the detection range, the concentrations of the two ions and the fluorescence signals are in good linear relationship. The application has good specificity for the two ions, and various metal ions such as potassium, copper, nickel, magnesium and calcium have no interference effect on the detection. The detection limits of the mercury ions and the lead ions are 4.094 nM and 3.22 nM respectively, and the whole detection process can be completed at room temperature. The result can be directly obtained through a fluorescence spectrophotometer. The test of the actual tap water sample has been completed, the detection process is convenient and fast, and the method can be used for detecting the mercury ions and the lead ions in actual water bodies.

The present application belongs to the technical field of aluminum salt determination of injection products in the pharmaceutical industry, and particularly relates to a method for determining aluminum salt in peritoneal dialysis fluid by using a fluorescence spectrophotometer. 3+ The complex formed in ethyl acetate has good stability and strong fluorescence intensity, and the sample is treated with concentrated hydrochloric acid and extracted with 1,1-dimethylpropanone (MIPK for short), so that the interference of transition metal impurity ions is eliminated. At the same time, the special determination parameters are specified, and the method after the research is verified well, and meets the aluminum salt inspection of peritoneal dialysis fluid products.

To provide an agent which does not utilize the toxicity of metal ions and exhibits one or two or more of high antibacterial, antifungal and antiviral properties in spite of a light color tone.SOLUTION: An agent comprising La (OH) 3, La2O2CO3, and ZnO, wherein a ratio (I (384) / I (560)) of a fluorescence emission intensity (I (560)) at a wave length of 365nm to a fluorescence emission intensity (I (384)) at a wave length of 560nm is 0.40 or more when the agent is irradiated with light having a wave length of 384nm and subjected to spectrofluorometric analysis, and the agent is selected from antibacterial agents, antifungal agents, and antiviral agents.SELECTED DRAWING: None

The utility model is suitable for the technical field of measurement, and provides controllable illumination equipment matched with a fluorescence spectrophotometer and a fluorescence spectrophotometric measurement system. The equipment comprises a shell provided with a clamping part, and the clamping part is used for being clamped with a sample placement bin of the spectrophotometer, so that the shell and the spectrophotometer integrally form a closed shading environment for a sample to be detected; the light-emitting panel is embedded in the shell and is used for generating radiation light rays for irradiating the sample to be detected; the light shielding plate is movably connected with the shell and used for changing the area, exposed in the external environment, of the light-emitting panel so as to shield the light-emitting panel; and a control panel. According to the application, the to-be-tested sample is not interfered by the external environment in the catalysis or fluorescence excitation process, the packaging of the original sample placing bin does not need to be damaged, the to-be-tested substance is subjected to light source irradiation in a convenient manner, the in-situ spectrophotometric test is provided, and the measurement precision and the operation convenience of the substance fluorescence phenomenon are improved.

The invention discloses a fluorescence detection method for exosome drug loading efficiency based on low-speed centrifugal separation. The method comprises the following steps: separating an exosome from a biological sample through low-speed centrifugation; labeling the drug with a fluorescent dye, and carrying the drug through a co-incubation or ultrasonic method; purifying the drug-loaded exosome by adopting low-speed centrifugation; and measuring the fluorescence intensity by using a fluorospectro photometer, and calculating the drug loading efficiency through a standard curve. According to the method, equipment is simple, operation is convenient, and rapid and low-cost detection can be achieved; by adopting low-speed centrifugal separation of the exosome and combining a fluorescence labeling technology, the equipment dependence and the operation complexity are reduced, so that a conventional laboratory without ultracentrifugation and liquid chromatography equipment can also efficiently detect the drug loading efficiency of the exosome, thereby solving the problems of high equipment requirement and complicated operation in the prior art, and improving the detection efficiency of the exosome. Meanwhile, the method is simple in step and easy to implement, and professional training requirements are reduced.

The invention provides a method for detecting aflatoxin B1 based on a magnetic immunofluorescence probe, which comprises the following steps: according to the specific recognition principle of an AFB1 antibody to AFB1, competitively recognizing AFB1 and a magnetic microballe-AFB1 conjugate in a sample through a QSH630-antibody conjugate, separating the QSH630-anti-AFB1-magnetic microsphere conjugate by using an external magnetic field, and detecting aflatoxin B1 by using a magnetic immunofluorescence probe. The fluorescence intensity of the red quantum dots in the system is reduced. The fluorescence intensity of the remaining red quantum dots is detected through a fluorospectro photometer, a linear standard curve of the fluorescence intensity and the concentration of the AFB1 is obtained, the concentration of the AFB1 and the fluorescence intensity are in a good linear correlation within the range of 1.25-4.75 pg / mL, and therefore high-sensitivity detection of the AFB1 is achieved. The invention establishes a simple, convenient, rapid and sensitive AFB1 specific analysis method, and has important significance on prevention and control of AFB1 pollution.

The present application provides a method for measuring zinc in a solution, the method comprising: preparing an indicator solution wherein the indicator solution comprises 8-hydroxy-5-quinolinesulfonic acid; introducing an indicator solution into the solution wherein the solution contains an amount of zinc and the introduction causes a fluorescence change in the solution in response to the indicator solution reacting with zinc; adding a moderator to the solution, wherein the moderator prevents the interferent from binding to the indicator; and measuring the amount of zinc in the solution by measuring a change in fluorescence intensity and comparing the change in intensity to a calibration curve generated based on conditions of the solution, where the conditions affect the fluorescence measurement of the solution, where the fluorescence intensity is proportional to the concentration of zinc in the solution, wherein low-range zinc detection is used, and the low-range zinc detection uses an emission scanning mode of a fluorospectro photometer. Other aspects are described and claimed.

The invention discloses a biological activity detection method and device based on deep learning, and relates to the technical field of biological detection.The biological activity detection method comprises the steps that firstly, a conjugated polymer probe containing triphenylamine and methylimidazole active units is prepared through Suzuki coupling reaction, and the aggregation-induced quenching effect is inhibited; carrying out standardized centrifugal impurity removal and dilution constant volume treatment on the sample, and collecting spectrum and RGB signals by combining a fluorospectro photometer and a portable device; and finally, realizing accurate detection and chain length distinguishing of the low-concentration pollutants by utilizing linear fitting and linear discriminant analysis algorithms. The device comprises a detection table, a multifunctional linkage mechanism and a self-adjusting protection mechanism are integrated, and accurate sample positioning, elastic buffering clamping and multi-angle posture adjustment are achieved through meshing transmission. The problems of low sensitivity, poor specificity, insufficient device suitability and the like of traditional detection are solved, the detection reliability and the scene suitability are improved, and the method is suitable for the fields of environment monitoring, food detection and the like.

This invention discloses a rapid detection method for lomefloxacin based on fluorescent carbon dots. The method applies fluorescent carbon dots to the detection of lomefloxacin drug concentration and includes the following steps: (1) preparing a carbon dot solution by mixing fluorescent carbon dots and water, then adding lomefloxacin standard solutions of different concentrations, using 395nm as the excitation wavelength, and measuring the emission intensity at 485nm wavelength using a fluorescence spectrophotometer to obtain a standard curve equation; (2) calculating the fluorescence intensity change value of the sample to be tested according to y = F - F0, and then substituting the measured fluorescence intensity change value of the sample to be tested into the standard curve to calculate the concentration of lomefloxacin to be tested. This invention provides a new pathway for the detection of lomefloxacin, with advantages such as convenient construction, high sensitivity, good specificity, and ease of use. It can be applied to the determination of drug concentration in lomefloxacin raw materials and their preparations, as well as in veterinary drug residues.

The application discloses a kind of in-situ measurement methods of aquatic primary productivity based on fluorescence excitation spectrum three-wavelength fitting model, belong to environmental monitoring and aquatic ecosystem technical field.The application includes: in-situ collection target water body water sample and filter to remove large particle impurities;Water sample is moved into fluorescence spectrophotometer sample cell, and after dark chamber balancing, the fluorescence intensity of 438nm, 485nm, 624nm three characteristic wavelengths is measured;According to water sample type, the integral area of fluorescence excitation spectrum is calculated using corresponding first-order linear fitting equation;Through the correlation model of integral area and primary productivity, the primary productivity of target water body is calculated.The application does not need sample pretreatment, simple and fast, measurement period is ≤30min, realizes in-situ non-invasive monitoring;Cover a variety of photosynthetic pigment characteristic absorption peak, wide application range, can tolerate a variety of stress conditions;Measurement precision is high, sensitivity is strong.

The application discloses a method for detecting sodium formaldehyde sulfoxylate based on nitrogen-doped graphene quantum dots ratio fluorescence, and belongs to the technical field of harmful compound detection in food. The method is characterized in that: a to-be-detected sample solution, a nitrogen-doped graphene quantum dots solution and a 1,4-naphthoquinone solution are mixed to react, after the reaction, ratio fluorescence response (I 420 / I 440 ) under an excitation wavelength of 360 nm is measured by a fluorescence spectrophotometer, the measured fluorescence intensity signal is introduced into a standard curve, and the concentration of sodium formaldehyde sulfoxylate in the to-be-detected sample is calculated and obtained. The application has the advantages of low cost, simple operation, visible detection result, no need of using large instruments and professional operators, and accurate and reliable detection result, and can be used for qualitative and quantitative detection of ivory white.

The application provides a coumarin-benzopyrylium salt derivative MI-BP-CC and a synthesis method and application thereof. The derivative MI-BP-CC has a Chinese name of 4-((4-chloro-7-(diethylamino)-2-oxo-2H-chromen-3-yl)-6-(diethylamino)-1,2,3,4-tetrahydroxanthylium chromenylium perchlorate and an English name of 4-((4-chloro-7-(diethylamino)-2-oxo-2H-chromen-3-yl)methylene)-6-(diethylamino)-1,2,3,4-(tetrahydroxanthylium)chromenylium perchlorate. The derivative MI-BP-CC is used as a fluorescent probe, and specific detection of trace viscosity in glycerol / ethanol (v / v) solutions of different proportions is realized through a fluorescence spectrophotometer. The detection method is simple, sensitive, rapid, and has high accuracy of detection results.