Magnetic molecularly imprinted polymer-fluorescence analysis method
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A technology of magnetic molecular imprinting and fluorescence analysis, which is applied in the field of analysis, can solve the problems such as the difficulty of preparing small molecule natural antibodies, and achieve the effect of good linear range and rapid detection
Active Publication Date: 2014-02-05
BEIJING PURKINJE GENERAL INSTR
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[0006] The present invention solves the problem that the small molecule natural antibody is difficult to prepare in t
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specific Embodiment approach 1
[0022] Specific embodiment 1: In this embodiment, the magnetic molecularly imprinted polymer-fluorescence analysis method is specifically carried out according to the following steps:
[0023] 1. FeCl 3 ·6H 2 O and FeSO 4 ·7H 2 Dissolve O in 100mL deionized water, under the condition of nitrogen protection, heat the water bath to the temperature of 60℃~70℃, add alkali solution until black precipitate is formed, keep it under stirring condition for 20min~40min, then add polyethylene glycol and oleic acid , to obtain the modified magnetic nanoparticles;
[0024] Among them, FeCl 3 ·6H 2 The mass of O is 2.7g, FeSO 4 ·7H 2 The quality of O is 1.7g, the concentration of alkali solution is 1.0mol / L, the quality of polyethylene glycol is 1g, and the volume of oleic acid is 2mL;
[0025] 2. Mix 1mmol permethazine, 4mmol methacrylic acid, 2mL solvent and 30mL porogen, sonicate for 30min, control the temperature at 4°C under dark conditions, keep it for 10h~12h, then add 2mL cr...
specific Embodiment approach 2
[0027] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the alkaline solution in step 1 is ammonia water or NaOH solution. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0028] Specific embodiment three: the difference between this embodiment and specific embodiment one is: in step two, the solvent is acetonitrile. Others are the same as in the first embodiment.
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Abstract
The invention discloses a magnetic molecularly imprinted polymer-fluorescence analysis method, relates to a rapid detection of pesticides, and aims to solve the problem of difficulty in preparation of small molecular natural antibody in the conventional quick detection method. The method comprises the following steps: 1, preparing polyethylene glycol and oleic acid-coated magnetic nanoparticles; 2, preparing a magnetic molecularly imprinted polymer of triazine pesticides; 3, determining a fluorescence signal by using a fluorescence spectrophotometer. The common natural antibody which is difficult to prepare in the normal quick detection method is substituted by using a magnetic molecularly imprinted biomimetric material; quick detection of a non-immune method of the triazinemicromoleuclar pesticides is technically realized through competitive combination of the pesticide molecules and the fluorescence probe on the magnetic imprinting. The method is used for quickly detecting the residue of the triazine pesticides.
Description
technical field [0001] The present invention relates to analytical methods. Background technique [0002] Triazine pesticides are important herbicides. Nearly 40 species have been developed, and more than 10 species are commonly used in my country. The annual output, trade share, and usage all rank first among all types of herbicides. With the widespread use of triazine herbicides, the harm caused by their improper use or excessive use has become increasingly apparent. The residues of triazine herbicides and their metabolites in soil and water not only have a great impact on the growth of subsequent crops, but also enter the food chain as they accumulate in crop products, causing chronic or acute poisoning of humans and animals. In particular, atrazine and other chlorotriazine-containing pesticides have been identified as environmental endocrine disruptors by many countries due to their mutagenic effects, and their residues have received widespread attention and attention. ...
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