Evolved immunoglobulin binding molecule, and its preparation method and uses

An immunoglobulin and binding molecule technology, applied in hybrid immunoglobulins, biochemical equipment and methods, botanical equipment and methods, etc., can solve the problem that the functional characteristics of LD5 are not further elaborated, and achieve improved sensitivity and accuracy. High performance, good effect, low cost effect
CN1966526AInactive Publication Date: 2007-05-23SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
Publication Date
2007-05-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relataes to novel evolutionary immunoglobulin binding molecules, as well as their preparation methods and applications. The invention discloses separated evolutionary immunoglobulin binding molecules, which are proteins with amino acid sequences as shown by SEQ ID NO : 1, or conservative variant proteins with immunoglobulin binding activity. The invention also discloses the gene encoding, genetic engineering preparation methods and applications of immunoglobulin binding molecules. The disclosed immunoglobulin molecule broad-spectrum combined with various immuneglobulin shows high immunoglobulin whole molecule binding activity, and can be used in large-scale purification of genetic engineering antibodies, purification of natural antibodies and monoclonal antibodies, enzyme-linked immunosorbent assay, and immuno-chromatography and immunohistochemical methods for immune antibody detection and diagnosis.
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Description

technical field

[0001] The present invention relates to a recombinant immunoglobulin binding molecule, in particular to an immunoglobulin binding molecule obtained by rearranging the Ig binding domain of Staphylococcus aureus protein and the surface protein of Streptococcus majoris, its preparation method and application. Background technique

[0002] Immunoglobulin-binding molecules (Ig binding proteins, IBP) are a class of proteins expressed on the surface of bacteria that can bind to specific parts of host Ig, regulate the host's immune response to bacteria, and are one of the important pathogenic factors of bacteria. Three main types of IBP have been studied the most: Staphylococcus aureus protein A (SpA), human streptococci (groups C and G) protein G (SpG) and surface protein L (PpL) of some Peptostreptococci magna.

[0003] SpA has a molecular weight of about 57KDa and is composed of about 524 amino acids. Its extracellular part contains five Ig-binding domains with a ...

Claims

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