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Preparation of red fluorescent carbon nanodots, and method used for detecting ferric ions and ascorbic acid

A carbon nanodot, red fluorescence technology, applied in fluorescence/phosphorescence, chemical instruments and methods, measurement devices, etc., can solve problems such as detection limitations, and achieve the effect of simple and easy test method, high sensitivity and good selectivity

Active Publication Date: 2017-08-15
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide an application of using carbon dot surface functional groups to detect ferric ions in order to solve the limitations of the detection of molecules and ions in the existing fluorescence detection technology.

Method used

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  • Preparation of red fluorescent carbon nanodots, and method used for detecting ferric ions and ascorbic acid
  • Preparation of red fluorescent carbon nanodots, and method used for detecting ferric ions and ascorbic acid
  • Preparation of red fluorescent carbon nanodots, and method used for detecting ferric ions and ascorbic acid

Examples

Experimental program
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Effect test

example 1

[0042] The carbon quantum dots used in the present invention are prepared by the following steps:

[0043] (1) Add 0.58g of citric acid, 0.76g of thiourea and 10mL of acetone into a 20mL reactor, and react in an oven at 160°C for 8 hours.

[0044] (2) Take out the reacted solution and place it in a centrifuge tube, set the rotation speed at 9000 rpm, and centrifuge for 10 minutes to remove the insoluble matter from the reaction residue.

[0045] (3) An eluent with a volume ratio of petroleum ether and ethyl acetate of 3:1 was prepared to elute the obtained carbon nanodots, and the lower layer of precipitate was removed, and dried in an oven at 60°C.

[0046] (4) Grinding the dried solid into powder with a mortar to obtain carbon dots with reddish-brown powder as red fluorescence.

[0047] (5) A carbon quantum dot solution of 20 μg / mL was prepared, and its fluorescence properties were tested by a fluorescence spectrophotometer, and the optimum excitation wavelength was obtaine...

example 2

[0057] (1) A 20 μg / mL carbon quantum dot solution was prepared, and its fluorescence properties were tested by a fluorescence spectrophotometer, and the optimal excitation wavelength was obtained as 560 nm.

[0058] (2) Add 3 mL of the carbon quantum dot solution prepared above into the quartz fluorescence cuvette.

[0059] (3) Use a pipette gun to transfer FeCl 3 The solution was added to the above-mentioned carbon quantum dot solution so that the final concentration was 4 μM, stirred evenly with a pipette, and allowed to stand for 1 minute.

[0060] (4) Measure the fluorescence intensity of the above mixed solution with a fluorescence spectrophotometer, the excitation wavelength is selected as 560nm, and record the fluorescence intensity of the solution.

[0061] (5) Add ascorbic acid solutions of different concentrations to the mixed solution of carbon dots and ferric ions so that the final concentration of ascorbic acid is 0-2 μM, stir evenly with a pipette, and let stand...

example 3

[0069] (1) Add 10 μL of 1 mM Fe to 3 mL of carbon quantum dot solution containing 20 μg / mL 3+ , Li + 、Na + 、K + 、Ag + , Zn 2+ , Pb 2+ 、Ni 2+ , Mn 2+ , Fe 2+ 、Cu 2+ 、Co 2+ 、Cd 2+ 、Au 3+ 、Cr 3+ solution to obtain different mixed solutions, stirred evenly and allowed to stand for 1 minute.

[0070] (2) Measure the fluorescence intensity of the above mixed solution with a fluorescence spectrophotometer, select the excitation wavelength as 560nm, and record the change ΔF of the fluorescence intensity of the solution.

[0071] (3) from Figure 8 It can be seen that the carbon dot solution has good selectivity to ferric ions.

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Abstract

The invention discloses preparation of red fluorescent carbon nanodots, and a method used for detecting ferric ions and ascorbic acid, and belongs to the field of metal ion detection. According to the preparation, citric acid, thiourea, and acetone are taken as raw materials, are delivered into a hydro-thermal reaction kettle, and are reacted for 8h at 160 DEG C so as to obtain the red fluorescent carbon nanodots. The method comprises following steps: a carbon nanodot solution is prepared; ferric ion solutions of different concentrations are added, and the change of the fluorescence intensity is detected using a fluorospectro photometer; a curve used for representing the change of fluorescence intensity with ferric ion concentration is drawn, and fitting is carried out so as to obtain a fitting formula; reduction of ferric irons can be realized with ascorbic acid, so that ascorbic acid solutions of different concentrations are added to recovery fluorescence, a curve used for representing the change of fluorescence intensity with ascorbic acid concentration is drawn, and fitting is carried out. The method can be used for detecting the concentrations of iron ions and ascorbic acid, and is simple and convenient.

Description

technical field [0001] The invention belongs to the field of metal ion detection, in particular to a method for preparing and detecting ferric ion and ascorbic acid by using red fluorescent carbon quantum dots. Background technique [0002] Carbon dots are a new type of fluorescent nanomaterials with properties such as multicolor luminescence, low toxicity, superior biocompatibility and good photostability. Therefore, carbon dots have more and more applications in ion detection, biological imaging and photoelectric conversion. [0003] Iron ion is an indispensable element in living systems and plays an important role in many life processes. Iron stone constitutes an important component of hemoglobin, myoglobin and various enzymes. If iron is lacking in the body, it will affect the synthesis of hemoglobin and myoglobin, and reduce the activity of some enzymes, which will cause many physiological changes. disease. Therefore, maintaining a certain level of iron ions in the b...

Claims

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Application Information

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IPC IPC(8): C09K11/65G01N21/64
CPCC09K11/65G01N21/643G01N2021/6432
Inventor 苗湘孙再成
Owner BEIJING UNIV OF TECH
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