Detecting method for aflatoxins

Abstract

The invention relates to a assaying method for the aflatoxin, specifically saying, it is a method of adopting immunity affinity column and fluorophotometer prepared by monoclonal antibody of aflatoxin to assayt the aflatoxin in the food, which belongs to biocytology and microbacteria toxic metabololic products assaying method technical field.The method is as following: first, preparing monoclonal antibody by the steps involving: immunizing mouse, cell fusion, screening crossing tumour and obtaining the cell line of the crossing tumourc, gathering monoclonal antibody, etc, then using the abovementioned monoclonal antibody to prepare the immunoaffinity column, last, after milling the food and feed, extracting the aflatoxin out of it with methanol / water solution, after filtering, letting the liquid sample flow through the affinity column to be refined, successively, eluting the AFT from the affinity column with methanol, and assaying the content of aflatoxin in the sample with a fluorophotometer.

Description

Technical field [0001] The present invention relates to a method for detecting aflatoxin, more specifically, it is a method for detecting aflatoxin in food and feed by using an immunoaffinity column prepared by monoclonal antibody of aflatoxin and a fluorometer. It belongs to biological cells The field of science and technology for the detection of toxic metabolites of microorganisms. Background technique [0002] Aflatoxin is a group of secondary metabolic toxic products with similar chemical structures of common molds, Aspergillus flarus and A. parasiticus. AFB has been isolated and identified 1 , AFB 2 , AFG 1 , AFG 2 , AFM 1 Wait 12 kinds. Aflatoxins are often found in various nuts such as peanuts and walnuts. They are also often found in soybeans, corn, rice, milk, dairy products, edible oils and grain and oil products, especially in humid tropical and subtropical areas where AFT pollution More serious. [0003] Aflatoxin (AFT) has a strong damaging effect on human and anima...

AI Technical Summary

Problems solved by technology

Although TLC is simple and economical, it has many steps and low sensitivity

HPLC has high sensitivity, but the sample processing is cumbersome, the operation is complicated, and the instrument is expensive

ELISA has poor repeatability and reagent life is short

In addition, these determination methods need to use highly toxic AFT as a standard substance during operation, and toxic and odorous organic solvents are required for pretreatment. These substances not only poison operators, but also pollute the environment

At present, the ELISA kits used in the detection of AFT in my country rely on imports, which are expensive and the detection cost is quite high. Therefore, under the influence of heavy economic burden, most enterprises do not pay attention to the supervision and detection of AFT.