The invention discloses a rabbit
monoclonal antibody preparation method. The method comprises the following steps of 1, preparing immune serum; 2, affine purifying an
antibody; 3, obtaining a
mass spectrum; 4, obtaining an
antibody variable region gene spectrum; 5, obtaining the
monoclonal antibody, wherein, in step 1, heteromorphic rabbits with bas mutations and wild parents b9 are selected, subcutaneous
inoculation of freund's
complete adjuvant on the rabbits, immunization is performed
every three days, immunization is performed three times, injection is performed one time two weeks after immunization, one month after immunization, NG-XMTTM
protein is subjected to intravenous injection at the tails of the rabbits, a sterile small
test tube is used for collecting immunized rabbit blood, after marking, the rabbits are delivered to a preparation room, unimmunized rat serum is adopted as control, ELISA is adopted for determining antibody valence, the ELISA is used for determining the OD490 light absorption value quantification and qualitatively judging whether or not the immune serum is positive, and the positive immune serum is selected; the method is more efficient and rapider andhas high affinity.