2 results about "Complete adjuvant" patented technology

The application discloses a specific antibody targeting a lysine K1106 site of an ITSN1-S protein lactylation and a preparation method and application thereof, and the preparation method comprises the following steps: mixing and emulsifying an ITSN1-S-K1106 lactylation antigen with Freund's complete adjuvant and Freund's incomplete adjuvant respectively to obtain a first immunization liquid drop and a booster immunization liquid drop, performing animal immunization by using the first immunization liquid drop and the booster immunization liquid drop respectively, and separating the specific antibody targeting the lysine K1106 site of the ITSN1-S protein lactylation from blood of the immunized animal.The antibody provided by the application can specifically detect the lactylation modification condition of the human ITSN1-S protein from the cell and tissue levels in actual application, and is helpful to explore the action mechanism of post-translational modification of the ITSN1-S protein in tumor proliferation, migration and the like, and provides a potential action target for clinical diagnosis and treatment of tumor diseases.

This invention utilizes an insect-baculovirus expression system to prepare the recombinant protein SADS-CoV-S of porcine acute diarrhea syndrome coronavirus, which is then used to prepare egg yolk antibodies. By optimizing the composition of the subunit vaccine composition for immunizing laying hens, IL-2 and ZnCl2 solution are added as immunostimulants to the conventional adjuvant, increasing the antibody concentration in the egg yolk antibody extract and improving the neutralizing antibody titer and protective efficacy. When IL-2 and zinc chloride aqueous solution are added to the immunization composition simultaneously, the resulting egg yolk antibody aqueous solution exhibits the highest protein concentration, and at the same protein concentration, the egg yolk antibody has the highest neutralizing titer. Compared to the immunization composition using Freund's complete adjuvant alone, the neutralizing titer is increased by more than 60 times.