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Multiplex flow type detection device for micro-droplet fluorescence image and spectrum scanning

A fluorescent image and spectral scanning technology, which is applied in the field of optical detection, can solve the problem of not being able to detect the fluorescence spectrum and fluorescence image information of micro-droplets at the same time, and achieve the effect of improving the acquisition speed and detection throughput

Inactive Publication Date: 2016-12-14
TSINGHUA UNIV
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Problems solved by technology

[0006] In order to overcome the current problem that the fluorescence spectrum and fluorescence image information of micro-droplets cannot be detected at the same time, and to meet the application requirements of fluorescence multiplexing coding technology in micro-droplets, the present invention provides a method for micro-droplet fluorescence image and spectral scanning multiplexing flow detection device

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Embodiment Construction

[0018] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments. It should be emphasized that the following description is only exemplary and not intended to limit the scope of the invention and its application.

[0019] Microdroplets, as microreactors, have been successfully used in protein crystallization, material synthesis, molecular and cell biology, and analytical chemistry. The specific embodiment of the present invention will be further described below in conjunction with the laser-induced fluorescence detection in micro-droplets.

[0020] One embodiment is that the microfluidic system includes a water phase input device, an oil phase input device and a microchip for generating and focusing microdroplets 1 .

[0021] Step 1) Processing of microchips:

[0022] The processing of the microchips is done in a clean process room. The photoresist mold is processed by MEMS lithography. After cleaning and dr...

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Abstract

The invention relates to a multiplex flow type detection device for micro-droplet fluorescence image and spectrum scanning. A flowing focusing principle is utilized to generate micro-droplet in a microchip; a contracting runner is adopted for realizing the focusing of the micro-droplet; the laser emitted from a laser device is expanded by an expanding mirror and then is reflected by a dichroic mirror; an objective lens is adopted for focusing the single micro-droplet in the contracting runner; after a fluorescent material in the micro-droplet is excited, the emitted fluorescent light is collected by the objective lens and then passes through the dichroic mirror and is split into two beams by a beam splitter mirror; one beam passes through a light filter and then is imaged on EMCCD, and the fluorescence image is recorded; after the other beam passes through a transmission type diffraction grating, the light in different wavelengths is diffracted to different directions so as to form a frequency band; a fluorospectro photometer is used for collecting and realizing the spectrum scanning. The device provided by the invention can be used for realizing the multiplex flow type detection for micro-droplet fluorescence image and spectrum scanning in the microchip runner, the complete optical information of the micro-droplet can be acquired and the application requirement for the fluorescence multiplex encoding technique in the micro-droplet can be met.

Description

technical field [0001] The invention belongs to the technical field of optical detection, in particular to a multiplexing flow detection device for micro-droplet fluorescence image and spectrum scanning. Background technique [0002] Droplet microfluidics is a micro-total analysis technology emerging in the 21st century. In 2001, Quake et al. used a T-channel microfluidic chip to form monodisperse droplets for the first time. This bottom-up droplet generation method significantly improved the operability of droplets and promoted the development of droplet microfluidics. Rapid development (Thorsen T, Roberts R W, Arnold F H, et al. Dynamic pattern formation in a vesicle-generating microfluidic device [J]. Physical review letters, 2001, 86(18): 4163.). As a microreactor, microdroplets have the advantages of no cross-contamination, high generation frequency, fast response speed, and matching single-cell analysis. At present, the chip on-line analysis technology for micro-drop...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6417
Inventor 任大海王斌尤政
Owner TSINGHUA UNIV
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