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Method for fast detecting pesticide residue

A pesticide residue and rapid technology, which is used in the preparation of test samples, material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of inability to quantitatively detect organophosphorus pesticides, difficult to store plant esterases, and high prices. The effect of less dosage and testing equipment, easy promotion and application, and easy operation

Active Publication Date: 2010-07-14
LUZHOU LAOJIAO GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The shortcoming of this method is: (1) the price of plant esterase is comparatively expensive; (2) plant esterase is not easy to preserve, needs to preserve under freezing condition; (3) this method can only realize qualitative detection DMMP, can't to organic phosphorus Quantitative detection of pesticides

Method used

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  • Method for fast detecting pesticide residue

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A method for rapid detection of organophosphorus pesticide residues, the specific method steps are as follows:

[0041] (1) Preparation of porphyrin solution

[0042] Taking tetraphenyl pentafluorozinc porphyrin as raw material, selecting organic solvent acetonitrile as fixed solvent, according to the mass of porphyrin: the volume ratio of organic solvent acetonitrile is a ratio of 1: 259.0, adding tetraphenyl pentafluorozinc porphyrin to organic solvent. In the solvent acetonitrile, after stirring and dissolving, a tetraphenyl pentafluorozinc porphyrin solution with a concentration of 0.004 g / L was prepared. Store in the refrigerator and store at 3°C ​​in the dark for future use.

[0043] (2) Preparation of known organophosphorus pesticide solutions

[0044] 1) Preparation of pesticide mother liquor

[0045] Select commercially available organophosphorus pesticide chlorpyrifos, add it to the organic solvent acetonitrile, stir evenly, the volume ratio of its organoph...

Embodiment 2

[0061] A method for rapid detection of organophosphorus pesticide residues, the concrete method steps of which are the same as those in Embodiment 1, wherein:

[0062] In the (1) step: take tetraphenylzinc porphyrin as raw material, by the quality of porphyrin: the volume ratio of organic solvent acetonitrile is the ratio of 1: 295.5, and the obtained concentration is the tetraphenylzinc porphyrin of 0.003g / L Phosphate solution. Store at 3.5°C in the dark.

[0063] In the (2)-1) step: the organophosphorus pesticide is dichlorvos, and its organophosphorus pesticide: the volume ratio of the organic solvent acetonitrile is 1:10 12 , the preparation concentration is 1×10 -4 μl / L of dichlorvos pesticide stock solution.

[0064] In the (2)-2) step: the volume of the porphyrin solution prepared in the (1) step: the volume ratio of the dichlorvos pesticide mother liquor prepared in the (2)-1) step is 1: 2, and the concentration is 4 × 10 -6 μl / L, 4×10 -5 μl / L, 4×10 -4 μl / L, 4×1...

Embodiment 3

[0071] A method for rapid detection of organophosphorus pesticide residues, the concrete method steps of which are the same as those in Embodiment 1, wherein:

[0072]In the (1) step: take tetraphenyl europium porphyrin as raw material, by the quality of porphyrin: the volume ratio of organic solvent acetonitrile is the ratio of 1: 262.0, and the obtained concentration is the tetraphenyl europium porphyrin of 0.004g / L Phosphate solution. Store at 4°C protected from light.

[0073] In the (2)-1) step: the organophosphorus pesticide is ammonium phosphine, and its organophosphorus pesticide: the volume ratio of the organic solvent acetonitrile is 1:10 10 , the preparation concentration is 1×10 -2 μl / L of Aminophos pesticide stock solution.

[0074] In the (2)-2) step: the volume of the porphyrin solution prepared in the (1) step: the volume ratio of the hydrophosphine pesticide mother liquor prepared in the (2)-1) step is 1: 2.5, and the concentration 5×10 -7 μl / L, 5×10 -6 ...

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Abstract

The invention provides a method for fast detecting pesticide residue, which relates to a fluoroscopic detection method of organic phosphor pesticide. The invention utilizes a fluorescence spectrophotometer with program, uses porphyrin compounds as fluorescence indicating agents and developers, and detects the organic phosphor pesticide in the liquid state porphyrin detection solution through preparing a porphyrin solution, an organic phosphor pesticide solution and a liquid state detection solution. The invention has the characteristics of high detection sensitivity, low detection limitation, high accuracy, simple and convenient operation, convenient and fast in-site detection, high economical efficiency, convenient popularization and application and the like. The invention can be widely used for the trace amount detection of foodgrain, food and the like which easily contain the organic phosphor pesticide residue, and can also be used in the security assessment of food such as vegetables, fruit and the like and the environment detection.

Description

technical field [0001] The invention belongs to the technical field of detection of chemical pesticides, and particularly relates to a fluorescence detection method for organophosphorus pesticides. technical background [0002] Organophosphorus pesticides are the most widely used chemical pesticides, and play an important role in agricultural production as the main means of production for the prevention and control of crop diseases and insect pests. The use of organophosphorus pesticides has greatly improved the yield of crops, but it not only brings economic benefits to human beings, but also causes harm to people's health and the external ecological environment. Organophosphorus pesticides remaining in the environment will not only affect various functions of the human body, but also pose potential harm to the human nervous system and other functional structures of the system, and even reduce the immune function of the body. carcinogenic, teratogenic, mutagenic). Since t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/28
Inventor 张良张明法焕宝张宿义侯长军霍丹群沈才洪傅深娜
Owner LUZHOU LAOJIAO GRP CO LTD
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