Fluorescence spectrum identification method of Fushunliao schisandra chinensis
A technology of Liao Schisandra and fluorescence spectrum, which is applied in the field of fluorescence spectrum identification, can solve problems such as insufficient recognition ability, inability to identify Fushun Liao Schisandra alone, and lack of quality control methods for Fushun Liao Schisandra, and achieve the effect of enhanced protection
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[0022] A. Establish a two-dimensional correlation synchronous fluorescence map of Fushun Liao Schisandra:
[0023] Take 10g of Schisandra schisandra fruit sample in Fushun and make it into a uniform powder. Weigh 0.05g of the powder and add it to 20ml of absolute ethanol, ultrasonically treat it for 30min, leave it to cool at room temperature and then centrifuge it. Take the centrifuged supernatant and filter it with a 0.45μm membrane Filter, discard the initial filtrate, take the subsequent filtrate as the analysis solution, scan according to the following scanning conditions, and obtain figure 1 The three-dimensional fluorescence map shown:
[0024] Excitation wavelength λ ex : 200-700nm;
[0025] Emission wavelength λ em : 200-800nm;
[0026] Voltage: 500V;
[0027] Scanning speed (SP): 12000nm / min;
[0028] Excitation and emission scanning slit: 5nm;
[0029] Scan step size: 5nm;
[0030] The three-dimensional fluorescence spectrum was exported by FL Solutions 2.1 ...
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