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Fluorescence spectrum identification method of Fushunliao schisandra chinensis

A technology of Liao Schisandra and fluorescence spectrum, which is applied in the field of fluorescence spectrum identification, can solve problems such as insufficient recognition ability, inability to identify Fushun Liao Schisandra alone, and lack of quality control methods for Fushun Liao Schisandra, and achieve the effect of enhanced protection

Inactive Publication Date: 2013-05-22
HEBEI UNIVERSITY
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Problems solved by technology

[0003] The existing spectral identification methods for Schisandra chinensis are mainly aimed at the identification of Schisandra chinensis from the north and south, but the recognition ability of Schisandra chinensis from a certain place of origin is obviously not enough
Fushun Liao Schisandra is an authentic medicinal material in Liaoning. It is mainly distributed in the eastern mountainous areas of Liaoning Province. It is famous for its thin skin, thick flesh, large grains, high medicinal ingredients, and full of pungent, sweet, sour, bitter, salty and five flavors. It is known as the "Five Flavors of Liao" In 2008, the General Administration of Quality Supervision, Inspection and Quarantine officially approved the implementation of national geographical indication product protection for Fushun Schisandra chinensis. The scope of protection covers Xinbin Manchu Autonomous County, Qingyuan Manchu Autonomous County, Fushun County, Shuncheng District, Wanghua District and Economic Development Zone Seven counties and districts currently govern the administrative areas. Due to the differences in chemical composition, content and efficacy of Schisandra from different origins, it is still impossible to identify Fushun Liao Schisandra separately, and there is a lack of information on Fushun Liao Schisandra. quality control means

Method used

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  • Fluorescence spectrum identification method of Fushunliao schisandra chinensis
  • Fluorescence spectrum identification method of Fushunliao schisandra chinensis
  • Fluorescence spectrum identification method of Fushunliao schisandra chinensis

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Embodiment 1

[0022] A. Establish a two-dimensional correlation synchronous fluorescence map of Fushun Liao Schisandra:

[0023] Take 10g of Schisandra schisandra fruit sample in Fushun and make it into a uniform powder. Weigh 0.05g of the powder and add it to 20ml of absolute ethanol, ultrasonically treat it for 30min, leave it to cool at room temperature and then centrifuge it. Take the centrifuged supernatant and filter it with a 0.45μm membrane Filter, discard the initial filtrate, take the subsequent filtrate as the analysis solution, scan according to the following scanning conditions, and obtain figure 1 The three-dimensional fluorescence map shown:

[0024] Excitation wavelength λ ex : 200-700nm;

[0025] Emission wavelength λ em : 200-800nm;

[0026] Voltage: 500V;

[0027] Scanning speed (SP): 12000nm / min;

[0028] Excitation and emission scanning slit: 5nm;

[0029] Scan step size: 5nm;

[0030] The three-dimensional fluorescence spectrum was exported by FL Solutions 2.1 ...

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Abstract

The invention discloses a fluorescence spectrum identification method of Fushunliao schisandra chinensis. The fluorescence spectrum identification method comprises the following steps of: firstly, obtaining a three-dimensional fluorescence spectrum of the Fushunliao schisandra chinensis and deriving the three-dimensional fluorescence spectrum in a data file ASCII (American Standard Code for Information Interchange) format to obtain original data of the three-dimensional fluorescence spectrum; extracting constant wavelength synchronous fluorescence signal data of emission wavelength and excitation wavelength with different wavelength intervals; obtaining a synchronous dynamic spectrum disturbed by different wavelength intervals between the related emission wavelength and excitation wavelength according to the data, so as to establish a two-dimensional related synchronous fluorescence spectrum of the Fushunliao schisandra chinensis; establishing a two-dimensional related synchronous fluorescence spectrum of unknown schisandra chinensis of the source area according to the same method; and comparing the two-dimensional related synchronous fluorescence spectrum with the two-dimensional related synchronous fluorescence spectrum of the Fushunliao schisandra chinensis to judge whether the sample is the Fushunliao schisandra chinensis. According to the method disclosed by the invention, the Fushunliao schisandra chinensis which is used as a geographical indication product and other non-Fushunliao schisandra chinensis can be directly and visually identified to enhance the protection on the Fushunliao schisandra chinensis.

Description

technical field [0001] The invention relates to a fluorescence spectrum identification technology, in particular to a fluorescence spectrum identification method of Fushun Liao schisandra. Background technique [0002] The traditional Chinese medicine Schisandra is the dried and ripe fruit of the Magnolia plant Schisandra. Clinical studies have found that Schisandra chinensis can significantly reduce the level of serum alanine aminotransferase in patients with hepatitis, and has anti-cancer and anti-AIDS activities. Schisandra plants are divided into two genera of Schisandra (ie, Northern Schisandra) and Southern Schisandra. At present, the identification of Schisandra chinensis is mainly the identification of Schisandra chinensis from the north and the south, and the spectral identification methods mainly include ultraviolet spectrophotometry, second derivative spectral fluorescence spectroscopy, infrared spectroscopy and so on. Among them, fluorescence spectroscopy has t...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 陈培云李红莲赵志磊孙汉文王献友庞艳苹李小亭田宇斯
Owner HEBEI UNIVERSITY
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