Fluorescence detection method of polycyclic aromatic hydrocarbons (PAHs) in water body

Abstract

The invention relates to a fluorescence detection method of PAHs in a water body. The method includes processing a water sample, soaking a solid-phase extraction column and sieve plate with n-hexane, drying, grinding and shaking bamboo charcoal out, and adding the bamboo charcoal into the solid-phase extraction column after drying to constant weight to form a bamboo charcoal solid-phase extraction column; subjecting a detection water sample to the bamboo charcoal solid-phase extraction column and finally obtaining a test sample solution after elution through the n-hexane and nitrogen blowing; performing qualitative measurement, performing constant wavelength synchronous fluorescence spectrum mapping on the test sample solution, determining synchronous fluorescence spectrums of components, and comparing synchronous fluorescence characteristic peaks of PAH standards with tested samples for identification and rough determination of the PAHs; and performing the quantitative measurement, determining fluorescence intensity of components under the condition of the constant wavelength synchronous fluorescence spectrum mapping, performing quantitative analysis by the aid of regression equations of standard solutions through an external standard method, and calculating content of PAHs in the test sample solution. The fluorescence detection method has the advantages of being simple in device, rapid in operation, solvent-saving, accurate in quantification and the like.

Description

technical field [0001] The invention relates to a detection method for polycyclic aromatic hydrocarbons, in particular to a fluorescence detection method for polycyclic aromatic hydrocarbons in water. Background technique [0002] Polycyclic aromatic hydrocarbons are a class of persistent organic pollutants that are carcinogenic, teratogenic and mutagenic, and mainly exist in the atmosphere, water and soil. Today's global polycyclic aromatic hydrocarbon pollution is caused by the incomplete combustion of organic matter and fossil fuels, such as smoke from household and domestic stoves, industrial boilers, etc., or various industrial processes that produce and use tar, such as coking, petroleum cracking, and coal tar refining. , asphalt paving, etc. or various man-made open-air combustion engine fires or various motor vehicles and internal combustion engine exhaust. Although the content of such compounds in the environment is very low, they are extremely harmful to humans. H...

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Problems solved by technology

[0003] The above-mentioned analysis methods of polycyclic aromatic hydrocarbons generally include four process steps of sample extraction, enrichment and purification, separation and determination, such as "Journal of Analysis and Testing [J], 2009, 28(9): 1022-1026" Xiao Ronghui, Ma Jiping, The "gas chromatography-mass spectrometry technology" disclosed by Lu Wenhui et al. and the "high performance liquid chromatography separation and Fluorescence detection technology "and so on, all have strong separation and analysis capabilities, but they all have the following shortcomings: first, they all need cumbersome pretreatment operations, and the equipment is expensive, making it difficult to quickly screen; second, due to the Chromatographic separation increases the experimental steps and needs to be diluted with eluent, which will affect the detection sensitivity

[0006] The commercial C18 solid phase extraction column used in the previously reported solid phase extraction is relatively expensive and cumbersome to operate; the analysis and detection methods of samples are usually high performance liquid chromatography coupled with fluorescence spectroscopy and gas chromatography coupled with flame ionization or mass spectrometry , so the samples are required to undergo purification steps before analysis, which increases the total analysis time of the samples accordingly, and polycyclic aromatic hydrocarbons exist in trace amounts in water bodies, which will cause large losses after the purification steps and affect Accuracy of test results

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