Method for acquiring optimal variable angle synchronous fluorescent spectrum

Abstract

A method includes using obtained 3-D fluorescent spectrum data to draw out contour projection figure of 3-D fluorescent spectrum, selecting scan path, obtaining fluorescent intensity by method of 2-D and three times of convolution-interpolation to draw out a variable angle synchronous fluorescent spectrum chart, selecting scan path repeatedly to have a numbers of spectrum charts and obtaining optimum variable angle synchronous fluorescent spectrum chart and its optimum scan path by comparing those obtained charts.

Description

technical field [0001] A method for obtaining optimal variable-angle simultaneous fluorescence spectra using three-dimensional fluorescence spectral data, while also determining the optimal scanning path. Background technique [0002] Fluorescence analysis is an analytical technique widely used in various fields such as industry, agriculture, medicine, health, forensic identification and scientific research. The traditional fluorescence analysis method refers to the qualitative or quantitative analysis of substances using the two-dimensional emission (or excitation) spectrum of the characteristic fluorescence of the substance. Compared with two-dimensional emission (or excitation) spectra, three-dimensional fluorescence spectra, in which fluorescence intensity varies with excitation and emission wavelengths, provide more information. Therefore, the use of three-dimensional fluorescence spectrum analysis as an effective test method for studying the geochemical properties of ...

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Problems solved by technology

[0003] In view of this, when using the variable-angle synchronous fluorescence method to analyze multi-component substances, a variable-angle synchronous fluorescence spectrometer that scans along a specific scanning path is usually designed for a specific substance according to the needs of specific analysis, and this scanning path is The optimal scanning path that satisfies the analysis requirements, and this scanning path is currently obtained by a relatively blind trial and error method, which is not only time-consuming, but also restricted by factors such as instrument performance and the subjective experience of the test personnel, so it is largely This restricts the selection of the optimal scanning path and greatly affects the quality of the variable-angle synchronous fluorescence spectrum. At the same time, this experimental method is also time-consuming to implement.

If a variety of different types of substances are measured simultaneously on a fluorescence spectrometer, it is necessary to determine the corresponding optimal scanning path for each type of substance, which is more complicated and time-consuming, which limits the popularization of the fluorescence spectrometer to some extent.

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