Preparation method for monodispersed and low-biotoxicity gold nanorods, and use for detection of allergen
A low biological toxicity, gold nanorod technology, applied in the detection of allergens, the preparation of low biological toxicity gold nanorods, the field of monodispersion, can solve the problems of unfavorable popularization, high cost, complex testing and testing methods, etc. The effect of stable layer structure, reduced usage, and avoidance of micellar structure damage
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Embodiment 1
[0036] Preparation of gold seeds: add 5mL 0.2M cetyltrimethylammonium bromide (CTMAB) solution to the flask, stir, add 5mL 0.5mM HAuCl dropwise 4 solution, injected with 0.01M NaBH 4 Solution 0.6mL, react for 2min; preparation of gold nanorods: Add the newly prepared growth solution containing 2mL 0.2M CTMAB and 0.12mM 4mM silver nitrate into the Erlenmeyer flask, add 1mL 14mM sodium dodecylbenzenesulfonate, stir , add 2mL 1mM HAuCl 4 and 0.18mL 0.0394M ascorbic acid solution, then add 0.04mL of the gold seed solution prepared above, mix, heat the reaction in a water bath for 10h, cool, and filter to remove the precipitated CTMAB precipitate; modification of gold nanorods: pipette 2.5mL of the above-prepared Put gold nanorods in a centrifuge tube, add pH 7.0, 10nmol PBS buffer solution and peanut allergen probe DNA solution modified with sulfhydryl groups, mix, and place in the refrigerator for 12h; the determination of allergens: take the above prepared Add 250 μL of gold n...
Embodiment 2
[0038] Preparation of gold seeds: add 5mL 0.2M cetyltrimethylammonium bromide (CTMAB) solution to the flask, stir, add 5mL 0.5mM HAuCl dropwise 4 solution, injected with 0.01M NaBH 4 Solution 0.6mL, react for 2min; preparation of gold nanorods: Add the newly prepared growth solution containing 2mL 0.2M CTMAB and 0.1mM 4mM silver nitrate into the Erlenmeyer flask, add 0.8mL 14mM sodium hexadecylbenzenesulfonate, Stir and add 2 mL of 1 mM HAuCl 4 and 0.18mL 0.0394M ascorbic acid solution, then add 0.04mL of the gold seed solution prepared above, mix, heat the reaction in a water bath for 10h, cool, and filter to remove the precipitated CTMAB precipitate; modification of gold nanorods: pipette 2.5mL of the above-prepared Put gold nanorods in a centrifuge tube, add pH 7.0, 10nmol PBS buffer solution and shrimp allergen probe DNA solution modified with sulfhydryl groups, mix, and place in the refrigerator for 12h; the determination of allergens: take the above prepared Add 250 μL...
Embodiment 3
[0040] Preparation of gold seeds: add 5mL 0.2M cetyltrimethylammonium bromide (CTMAB) solution to the flask, stir, add 5mL 0.5mM HAuCl dropwise 4 solution, injected with 0.01M NaBH 4 Solution 0.6mL, reacted for 2min; preparation of gold nanorods: Add the newly prepared growth solution containing 2mL 0.16M CTMAB and 0.14mM 4mM silver nitrate to the Erlenmeyer flask, add 0.8mL 14mM sodium dodecylbenzenesulfonate, Stir and add 2 mL of 1 mM HAuCl 4 and 0.18mL 0.0394M ascorbic acid solution, then add 0.04mL of the gold seed solution prepared above, mix, heat the reaction in a water bath for 10h, cool, and filter to remove the precipitated CTMAB precipitate; modification of gold nanorods: pipette 2.5mL of the above-prepared Put gold nanorods in a centrifuge tube, add pH 7.0, 10nmol PBS buffer solution and shrimp allergen probe DNA solution modified with sulfhydryl groups, mix, and place in the refrigerator for 12h; the determination of allergens: take the above prepared Add 250 μL...
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