Preparation method for monodispersed and low-biotoxicity gold nanorods, and use for detection of allergen

A low biological toxicity, gold nanorod technology, applied in the detection of allergens, the preparation of low biological toxicity gold nanorods, the field of monodispersion, can solve the problems of unfavorable popularization, high cost, complex testing and testing methods, etc. The effect of stable layer structure, reduced usage, and avoidance of micellar structure damage

Active Publication Date: 2014-06-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a preparation method of monodisperse, low biotoxicity gold nanorods and a method for all

Method used

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  • Preparation method for monodispersed and low-biotoxicity gold nanorods, and use for detection of allergen

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Experimental program
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Effect test

Embodiment 1

[0036] Preparation of gold seeds: add 5mL 0.2M cetyltrimethylammonium bromide (CTMAB) solution to the flask, stir, add 5mL 0.5mM HAuCl dropwise 4 solution, injected with 0.01M NaBH 4 Solution 0.6mL, react for 2min; preparation of gold nanorods: Add the newly prepared growth solution containing 2mL 0.2M CTMAB and 0.12mM 4mM silver nitrate into the Erlenmeyer flask, add 1mL 14mM sodium dodecylbenzenesulfonate, stir , add 2mL 1mM HAuCl 4 and 0.18mL 0.0394M ascorbic acid solution, then add 0.04mL of the gold seed solution prepared above, mix, heat the reaction in a water bath for 10h, cool, and filter to remove the precipitated CTMAB precipitate; modification of gold nanorods: pipette 2.5mL of the above-prepared Put gold nanorods in a centrifuge tube, add pH 7.0, 10nmol PBS buffer solution and peanut allergen probe DNA solution modified with sulfhydryl groups, mix, and place in the refrigerator for 12h; the determination of allergens: take the above prepared Add 250 μL of gold n...

Embodiment 2

[0038] Preparation of gold seeds: add 5mL 0.2M cetyltrimethylammonium bromide (CTMAB) solution to the flask, stir, add 5mL 0.5mM HAuCl dropwise 4 solution, injected with 0.01M NaBH 4 Solution 0.6mL, react for 2min; preparation of gold nanorods: Add the newly prepared growth solution containing 2mL 0.2M CTMAB and 0.1mM 4mM silver nitrate into the Erlenmeyer flask, add 0.8mL 14mM sodium hexadecylbenzenesulfonate, Stir and add 2 mL of 1 mM HAuCl 4 and 0.18mL 0.0394M ascorbic acid solution, then add 0.04mL of the gold seed solution prepared above, mix, heat the reaction in a water bath for 10h, cool, and filter to remove the precipitated CTMAB precipitate; modification of gold nanorods: pipette 2.5mL of the above-prepared Put gold nanorods in a centrifuge tube, add pH 7.0, 10nmol PBS buffer solution and shrimp allergen probe DNA solution modified with sulfhydryl groups, mix, and place in the refrigerator for 12h; the determination of allergens: take the above prepared Add 250 μL...

Embodiment 3

[0040] Preparation of gold seeds: add 5mL 0.2M cetyltrimethylammonium bromide (CTMAB) solution to the flask, stir, add 5mL 0.5mM HAuCl dropwise 4 solution, injected with 0.01M NaBH 4 Solution 0.6mL, reacted for 2min; preparation of gold nanorods: Add the newly prepared growth solution containing 2mL 0.16M CTMAB and 0.14mM 4mM silver nitrate to the Erlenmeyer flask, add 0.8mL 14mM sodium dodecylbenzenesulfonate, Stir and add 2 mL of 1 mM HAuCl 4 and 0.18mL 0.0394M ascorbic acid solution, then add 0.04mL of the gold seed solution prepared above, mix, heat the reaction in a water bath for 10h, cool, and filter to remove the precipitated CTMAB precipitate; modification of gold nanorods: pipette 2.5mL of the above-prepared Put gold nanorods in a centrifuge tube, add pH 7.0, 10nmol PBS buffer solution and shrimp allergen probe DNA solution modified with sulfhydryl groups, mix, and place in the refrigerator for 12h; the determination of allergens: take the above prepared Add 250 μL...

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Abstract

The invention belongs to the chemical field, and provides a preparation method for monodispersed and low-biotoxicity gold nanorods, and use for detection of allergen. The preparation method comprises the steps of (1) adding cetyltrimethylammonium bromide (CTMBA) in a beaker, adding HAuCl4 and NaBH4 and stirring; (2) adding a growth solution and an anionic surfactant into the beaker, adding HAuCl4 and ascorbic acid with stirring, adding gold seeds prepared by the step (1), heating in a water bath, cooling, and filtering precipitated CTMAB; (3) taking the gold nanorods prepared by the step (2), adding a PBS solution and a probe DNA and putting in a refrigerator; and (4) taking a certain amount of the modified gold nanorods prepared by the step (3), adding a target DNA, measuring synchronous fluorescence and calculating the content of allergen according to a regression curve. According to the preparation method, the anionic surfactant is used as an additive, so that the usage amount of CTMAB is reduced. The prepared gold nanorods have good monodispersity and low biotoxicity. By using the gold nanorods in the detection of allergen, a detection limit and sensitivity of the method is obviously higher than that of a conventional technology.

Description

technical field [0001] The invention belongs to the field of chemical industry, and in particular relates to a preparation method of monodisperse and low biological toxicity gold nanorods and its use in the detection of allergens. Background technique [0002] Gold nanorods have excellent properties different from other materials (Jie Cao; EwanK.Galbrainth; Tong Sun, Sensors and Actuators B : Chemical, 2012, 169: 360-367), which has attracted much attention in the fields of biomedicine and analytical science, and is widely used in the fields of nano-devices, biomarkers, medical detection and surface-enhanced Raman (Seunghyun Lee; Lindsey J.E. Anderson; Courtney M. Payne; Jason H. Hafner, Langmuir, 2011, 27, 14748-14756). Recently, researchers found that gold nanorods have special resonant light scattering (Ning Bi; Ying Sun; Hanqi Zhang, Colloids and Surfaces B: Biointerfaces, 2010, 81: 249-254.), plasmon resonance light scattering based on gold nanorods ( PRLS) can determ...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 李在均黄颖娟
Owner JIANGNAN UNIV
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