A preparation method of monodisperse, low biotoxic gold nanorods for allergen detection

A low biological toxicity, gold nanorod technology, applied in the chemical industry, can solve the problems of unfavorable popularization, complicated test methods, and high cost, and achieve the effect of stable double-layer structure, avoiding the destruction of micellar structure, and reducing the amount of use

Active Publication Date: 2016-09-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a monodisperse, low biotoxicity gold nanorod for allergen detection in view of the existing food allergen detection method. Preparation

Method used

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  • A preparation method of monodisperse, low biotoxic gold nanorods for allergen detection

Examples

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Effect test

Embodiment 1

[0038] Gold seed preparation: add 5mL 0.2M cetyltrimethylammonium bromide (CTMAB) solution to the flask, stir, and add 5mL 0.5mM HAuCl dropwise 4 Solution, inject 0.01M NaBH 4 Solution 0.6mL, reaction for 2min; preparation of gold nanorods: add the newly prepared growth solution containing 2mL 0.2M CTMAB and 0.12mL 4mM silver nitrate into the Erlenmeyer flask, add 1mL 14mM sodium dodecylbenzene sulfonate and stir , Add 2mL 1mM HAuCl 4 And 0.18mL of 0.0394M ascorbic acid solution, and then add 0.04mL of the gold seed solution prepared above, mix, heat in a water bath for 10h, cool, filter to remove the precipitated CTMAB precipitate; modification of gold nanorods: pipette 2.5mL of the prepared above Add gold nanorods to a centrifuge tube, add pH 7.0, 10nmol PBS buffer solution and peanut allergen probe DNA solution modified with sulfhydryl groups, mix, and place in the refrigerator for 12 hours; determination of allergens: take the prepared above 250μL of gold nanorod solution fo...

Embodiment 2

[0040] Gold seed preparation: add 5mL 0.2M cetyltrimethylammonium bromide (CTMAB) solution to the flask, stir, and add 5mL 0.5mM HAuCl dropwise 4 Solution, inject 0.01M NaBH 4 Solution 0.6mL, reaction for 2min; preparation of gold nanorods: add the newly prepared growth solution containing 2mL 0.2M CTMAB and 0.1mL 4mM silver nitrate to the Erlenmeyer flask, add 0.8mL 14mM sodium cetylbenzene sulfonate, Stir, add 2mL 1mM HAuCl 4 And 0.18mL of 0.0394M ascorbic acid solution, and then add 0.04mL of the gold seed solution prepared above, mix, heat in a water bath for 10h, cool, filter to remove the precipitated CTMAB precipitate; modification of gold nanorods: pipette 2.5mL of the prepared above Put gold nanorods in a centrifuge tube, add pH 7.0, 10nmol PBS buffer solution and end-position modified sulfhydryl shrimp allergen probe DNA solution, mix, and place in the refrigerator for 12 hours; determination of allergen: take the prepared above 250μL of gold nanorod solution of modifi...

Embodiment 3

[0042] Gold seed preparation: add 5mL 0.2M cetyltrimethylammonium bromide (CTMAB) solution to the flask, stir, and add 5mL 0.5mM HAuCl dropwise 4 Solution, inject 0.01M NaBH 4 Solution 0.6mL, reaction for 2min; preparation of gold nanorods: add the newly prepared growth solution containing 2mL 0.16M CTMAB and 0.14mL 4mM silver nitrate into the Erlenmeyer flask, add 0.8mL 14mM sodium dodecylbenzene sulfonate, Stir, add 2mL 1mM HAuCl 4 And 0.18mL of 0.0394M ascorbic acid solution, and then add 0.04mL of the gold seed solution prepared above, mix, heat in a water bath for 10h, cool, filter to remove the precipitated CTMAB precipitate; modification of gold nanorods: pipette 2.5mL of the prepared above Put gold nanorods in a centrifuge tube, add pH 7.0, 10nmol PBS buffer solution and end-position modified sulfhydryl shrimp allergen probe DNA solution, mix, and place in the refrigerator for 12 hours; determination of allergen: take the prepared above 250μL of gold nanorod solution of ...

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Abstract

The invention belongs to the chemical field, and provides a preparation method for monodispersed and low-biotoxicity gold nanorods, and use for detection of allergen. The preparation method comprises the steps of (1) adding cetyltrimethylammonium bromide (CTMBA) in a beaker, adding HAuCl4 and NaBH4 and stirring; (2) adding a growth solution and an anionic surfactant into the beaker, adding HAuCl4 and ascorbic acid with stirring, adding gold seeds prepared by the step (1), heating in a water bath, cooling, and filtering precipitated CTMAB; (3) taking the gold nanorods prepared by the step (2), adding a PBS solution and a probe DNA and putting in a refrigerator; and (4) taking a certain amount of the modified gold nanorods prepared by the step (3), adding a target DNA, measuring synchronous fluorescence and calculating the content of allergen according to a regression curve. According to the preparation method, the anionic surfactant is used as an additive, so that the usage amount of CTMAB is reduced. The prepared gold nanorods have good monodispersity and low biotoxicity. By using the gold nanorods in the detection of allergen, a detection limit and sensitivity of the method is obviously higher than that of a conventional technology.

Description

Technical field [0001] The invention belongs to the field of chemical engineering, and particularly relates to a preparation method of monodisperse and low biological toxicity gold nanorods for allergen detection. Background technique [0002] Gold nanorods have anisotropic, tunable surface ionomer resonance wavelength and extremely high surface electric field strength enhancement effect. They have excellent properties that are different from other materials (Jie Cao; EwanK. Galbrainth; Tong Sun, Sensors and Actuators B : Chemical, 2012, 169: 360-367), which has attracted much attention in the fields of biomedicine and analytical science, and is widely used in the fields of nanodevices, biomarkers, medical detection and surface enhanced Raman (SeunghyunLee; Lindsey JE Anderson ; Courtney M. Payne; Jason H. Hafner, Langmuir, 2011, 27, 14748-14756). Recently, researchers discovered that gold nanorods have special resonance light scattering (Ning Bi; Ying Sun; Hanqi Zhang, Colloids...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 李在均黄颖娟
Owner JIANGNAN UNIV
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