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Fluorophototmeter capable of detecting light absorption or light transmission

A fluorescence photometer and a technology of fluorescence photometry, applied in the field of fluorescence photometry, can solve problems such as the reduction of fluorescence intensity

Inactive Publication Date: 2010-05-12
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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Problems solved by technology

It can be seen that different fluorescent substances have different fluorescence characteristics and concentration ranges in which characteristic fluorescence occurs, but when the concentration is high, inner filtering and self-absorption (reabsorbance) phenomena will occur, resulting in a decrease in fluorescence intensity

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  • Fluorophototmeter capable of detecting light absorption or light transmission
  • Fluorophototmeter capable of detecting light absorption or light transmission
  • Fluorophototmeter capable of detecting light absorption or light transmission

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Embodiment Construction

[0034] like figure 1Published Fluorescence Photometer. The fluorescence photometer includes a light source (generally a xenon lamp or a high-pressure mercury lamp with a wavelength of 200-900nm), an excitation light monochromator, an incident slit, a sample cell, an emission slit, an emission light monochromator, a detector, an amplifier, and a recorder. instrument, reflective slit and transmitted light reflectance system. A transmitted light reflectance system reflects transmitted light into a detector for detection of transmittance. The transmitted light reflection system may be a simple reflection mirror, a reflection prism, or a reflection mirror group or a reflection prism group. The specific position and setting of the transmitted light reflection system depends on the specific setting of the fluorescence photometer. The simplest way is to adjust the installation angle of the reflector group to reflect the transmitted light into the photodetector to achieve the detecti...

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Abstract

The invention provides a fluorophototmeter capable of detecting light absorption or light transmission, which is improved on the basis of the original fluorophototmeter; the fluorescence detection and light absorption or light transmission detection are combined to achieve the purposes that not only the sample fluorescence spectrum, such as three-dimensional fluorescence and synchronous fluorescence can be detected, but also the absorption spectrum of the detection sample to the light, such as light absorbency or light transmittance and the like can be detected; and meanwhile, the fluorophototmeter can conveniently realize correction to the three-dimensional fluorescence spectrum. On the basis of the existing fluorophototmeter, by simply adding a reflecting system or a new detector, the fluorophototmeter can detect the transmission light transmitting a sample cell, and realizes the purposes of the invention.

Description

technical field [0001] The invention protects a fluorescence photometer capable of detecting light absorption or light transmission. The fluorescence photometer belongs to a combined detector, and the detector includes two parts: an ultraviolet / visible spectrophotometer and a fluorescence photometer, and the detector can realize the two functions of the ultraviolet / visible spectrophotometer and the fluorescence photometer. At the same time, the photometer can conveniently realize the correction of the three-dimensional fluorescence spectrum. Background technique [0002] Three-dimensional fluorescence spectroscopy is a new analytical technique developed on the basis of fluorescence spectral analysis in the 1980s. The method of obtaining three-dimensional fluorescence data is generally to continuously scan the emission spectrum at different excitation wavelength positions, and use various plots The software visualizes it in the form of isometric three-dimensional fluorescenc...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/59G01N21/17
Inventor 刘俐李发生王立
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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