264 results about "Three dimensional fluorescence" patented technology

The invention discloses an ultraviolet fluorescence double-signal water quality monitoring device taking an LED (light emitting diode) as a light source and an application method of the device, and belongs to the field of environmental monitoring and water treatment. The ultraviolet fluorescence double-signal water quality monitoring device taking the LED as the light source consists of a sample collecting part and a detection part. The application method comprises the steps of selecting a deep ultraviolet LED lamp light source with specific wavelength and a photoelectric detector component according to a three-dimensional fluorescence atlas, detecting fluorescence intensity and ultraviolet absorption at the specific wavelength at the same time and calculating a ratio of the fluorescence intensity to the corresponding ultraviolet absorption. According to the device, no chemical reagent is consumed, monitoring of total concentration change of soluble organic matters in a water body can be realized, change of concentration of fluorescence ingredients such as proteins and humus can be reflected at the same time, and the sensitive, quick, efficient, economical, easy and information-rich on-line monitoring device is provided for the water treatment.

The invention is suitable for the field of microscope imaging, and provides a three-dimensional fluorescence nano microscope imaging method and a three-dimensional fluorescence nano microscope imaging system, and image equipment. The method comprises the following steps of: producing exciting light; converting the exciting light into a laminar exciting beam; making the laminar exciting beam act on a sample; detecting a fluorescent light emitted by a fluorescent label in the sample layer on which the laminar exciting beam acts; transversely positioning to acquire the two-dimensional position of the fluorescent label; axially positioning to acquire the axial position of the fluorescent label; three-dimensionally reconstructing, combining the two-dimensional position with the axial position to acquire a three-dimensional nano resolution image of the sample layer on which the laminar exciting beam acts; axially scanning to acquire the three-dimensional nano resolution images of different sample layers; and acquiring the three-dimensional nano resolution image of the complete sample. Through the three-dimensional fluorescence nano microscope imaging method and the three-dimensional fluorescence nano microscope imaging system, and the image equipment, the high-precision three-dimensional nano microscope imaging is realized by taking the laminar light beam as the exciting light, and combining with axial scanning, and the method, system and equipment are suitable for three-dimensional microscope imaging of the thick sample, such as cells in the biological field and the like, and solve the problems that the positioning accuracy of molecules in the thick sample is low and the sample is difficult to observe.

The invention discloses a discrete three-dimensional fluorescence spectrum-based phytoplankton identification and measurement method and a discrete three-dimensional fluorescence spectrum-based phytoplankton identification and measurement device. The measurement method comprises the following steps: selecting a characteristic fluorescence peak which corresponds to different photosynthetic pigments as an analytic target according to fingerprint characteristics of a phytoplankton fluorescence spectrum, maintaining the phytoplankton characteristic information in the three-dimensional fluorescence spectrum to the greatest degree, fitting a characteristic spectrum of the sample according to each characteristic spectrum in a standard discrete three-dimensional fluorescence spectrum library, and inverting the types and the content of the phytoplankton in the sample. According to the measurement device, a multiband light-emitting diode (LED) array serves as an excitation light source, and a multiband light filter group serves as a band selector for receiving fluorescence. The device is simple and compact in structure, small in size and convenient to carry and is suitable for identification and measurement of field phytoplankton, the phytoplankton identification and measurement method provided by the invention is combined, so that rapid classified measurement on the phytoplankton site can be realized.

The invention relates to a quickly judging method for underground water polluted by organic matter, which comprises the following steps: A) collecting underground water samples in, pollution source areas, the upstream, two sides and the downstream of the pollution source areas; B) filtering collected underground samples, and determining soluble organic carbon concentration; C) converging the three-dimensional fluorescence spectrum matrix data of all underground water samples so as to process parallel factor analysis; and D) judging that the underground water is not polluted if component acquired in the parallel factor analysis does not have fluorescence peaks within a range that an excitation wavelength is larger than or equal to 200nm and is less than or equal to 440nm; and an emission wavelength is larger than or equal to 280nm and is less than 380nm; judging that the underground water is polluted if the component acquired in the parallel factor analysis appears the fluorescence peaks within a range that the excitation wavelength is larger than or equal to 200nm and is less than or equal to 440nm; and the emission wavelength is larger than or equal to 280nm and is less than 380nm, wherein the larger the maximum fluorescence strength of the underground fluorescence component is, the severer the pollution degree of the underground is. According to the invention, the quickly judging method is characterized by little sample quantity, quick analysis and high sensitivity.

The invention relates to a real-time three-dimensional laser fluorescence microscopic imaging device. The real-time three-dimensional laser fluorescence microscopic imaging device is compatible with a traditional bright field illumination microscopic imaging working mode. The real-time three-dimensional laser fluorescence microscopic imaging device is divided into a laser excitation module, a bright field imaging illumination module, a digital detection module, an eyepiece observation module and a control module according to functions. A spatial light modulator is adopted to load a pyramid phase, so that a Gaussian-Bessel illumination light field can be generated at the backfield of a focusing objective lens. In a detection optical path, a twisted Dammann grating is additionally adopted so as to realize simultaneous imaging of a multi-surface object, fluorescence collected from the objective lens simultaneously images a plurality of axial planes to an electron-enhanced CCD detection surface through a multi-surface imaging technology, and therefore, real-time three-dimensional fluorescence imaging can be realized. The real-time three-dimensional fluorescence imaging technique has an important practical value in biological living tissue and living cell microscopic imaging.

The invention provides a method for correcting and calibrating three-dimensional fluorescence data of colored soluble organic matters. The method comprises the steps of setting measurement conditions such as three-dimensional fluorescence excitation, a wavelength generation range and a scanning speed; measuring a three-dimensional fluorescence spectrum of a sample; measuring a three-dimensional fluorescence spectrum of ultrapure water; performing Raman scattering correction by subtracting excitation-emission three-dimensional fluorescence spectrum of the ultrapure water from the excitation-emission three-dimensional fluorescence spectrum of the sample; assigning 0 to a triangular region of each three-dimensional fluorescence spectrum to perform Rayleigh scattering correction; performing calibration on the fluorescence intensity through the excitation wavelength of 350nm and the emission wavelength of 371-428nm of the ultrapure water; measuring an absorption spectrum of the sample to obtain the absorbency of the sample; performing inner filering effect correction on the three-dimensional fluorescence data according to the absorbency of the sample. According to the method disclosed by the invention, the influence of the inner filtering effect of the high-concentration sample on the fluorescence intensity can be eliminated without dilution of the sample.

The invention discloses a method for identifying baijiu of different brands by combining a three-dimensional fluorescence spectrum and a parallel factor, belonging to the technical field of baijiu identification. The method disclosed by the invention comprises the following steps: (1) obtaining the three-dimensional fluorescence spectrum data of baijiu of different brands by using a fluorescence spectrophotometer; (2) establishing an identification model of baijiu of different brands, namely decomposing the spectrum data by utilizing a parallel factor method, thus obtaining a loading matrix and a concentration score, and by taking the concentration score as input of a support vector machine, establishing an identification model of the brands of the baijiu; and (3) predicting the brands of the baijiu, specifically, for the predicted sample, performing testing to obtain the three-dimensional fluorescence spectrum data, and obtaining the concentration score by utilizing a genetic algorithm and by keeping the loading matrix invariable, inputting the concentration score into the identification model and outputting the predicted brand value. According to the method disclosed by the invention, a complicated chemical separation process is avoided, the method has the advantages of rapidness, safety, simplicity in operation, low price, nondestructive property and the like, and the identification model of baiju in different places of origin can be further established.

The invention discloses a method and apparatus for acquiring plant three-dimensional chlorophyll fluorescent image information, wherein the method comprises the steps of subjecting a plant to be measured to dark adaptive treatment, and acquiring, under induction of exciting light, the plant's chlorophyll fluorescent image and gray image information at different angles; pretreating the chlorophyll fluorescent image and gray image information, acquired at different angles, of the plant to be measured so as to obtain chlorophyll fluorescent image and gray image to be reconstructed; loading the chlorophyll fluorescent image and gray image to be reconstructed, reconstructing the chlorophyll fluorescent image and gray image of the plant to be measured, correcting the three-dimensional fluorescent image by using a three-dimensional gray image to obtain a final three-dimensional chlorophyll fluorescent image. By using the method and apparatus, a chlorophyll fluorescent image of a single leaf can be acquired, and heterogeneity of plant photosynthesis can be sensed in three-dimensional space sense.

The invention discloses a three-dimensional fluorescent differential super-resolution microscopic device which comprises a laser, an electric sample stage carrying a sample to be detected, and a microobjective for projecting light rays to the electric sample stage, wherein a polarizer for converting a beam emitted by the laser into linear polarized light, a first half wave plate for modulating a polarization direction of the linear polarized light, a space light modulation module for sequentially modulating a horizontal component and a vertical component of the beam and a scanning galvanometer system for light path deflection of circularly polarized light are sequentially arranged between the laser and the microobjective; the circularly polarized light emitted by the scanning galvanometer system is projected by the microobjective onto the sample to be detected; the device further comprises a detection system acquiring signal light emitted by the sample to be detected, and a computer controlling the space light modulation module and the scanning galvanometer system. The invention further discloses a microscopic method achieved on a basis of the three-dimensional fluorescent differential super-resolution microscopic device.

The invention relates to a method for quickly identifying refuse landfill percolate in the sewage water discharged into a sewage treatment plant, in particular to a method for quickly judging whether percolate is illegally discharged into the sewage treatment plant. The method comprises the following steps: taking residual active sludge in the sewage treatment plant, then carrying out centrifugation on the residual active sludge so as to obtain a supernatant liquid; carrying out filtration on the supernatant liquid by a filter membrane (0.45 mu m) so as to remove suspended matters in the supernatant liquid to obtain a sample; taking a point every 2nm and then carrying out three-dimensional fluorescence spectrum survey on the sample so as to obtain a numerical three-dimensional fluorogram under the conditions that the excitation wavelength is 200 to 500nm, the emission wavelength is 250 to 600nm, the slit width is 5nm, and the scanning speed is 1200nm/min, wherein in case the fluorescence peak of the sample only refers to fulvic acid-lile substances (Ex/Em: 240/440nm) and humic acid-like substances (Ex/Em: 330/420 nm), but without protein-like substances, the numerical three-dimensional fluorogram shows that the refuse landfill percolate exists in the sewage water discharged into the sewage treatment plant, otherwise, the numerical three-dimensional fluorogram shows that no refuse landfill percolate exists in the sewage water discharged into the sewage treatment plant. The method of the invention has the advantages of simpleness, speediness, less required samples, high sensitivity, low cost, and capability of being widely applicable for quickly judging whether percolate is discharged into a sewage treatment plant.

A method for distinguishing water bursting source of a coal mine comprises the specific steps as follows: 1, determining the hydrogeological characteristics of a target area; 2, conducting water sample collection and detection on the target area; 3, preliminarily analyzing the information of water samples in the target area; 4, determining the finger-print sensitive area in the three-dimensional fluorescence spectrum of DOM (Dissolved Organic Matter) in different water bearing layers in the target area; 5, establishing a hydrochemical characteristic information table of each water layer in the target area. The method provided by the invention is fast in distinguishing, and can establish the hydrochemical characteristics of different water bearing layers by utilizing the total content and the fluorescence characteristics of DOM in groundwater, so as to provide a technical method for distinguishing a water bursting source, provide hydrochemical characteristic data that can easily distinguish water bearing layers for water prevention and control, determine an accurate water bursting source for water shutoff in the process that water bursting happens in the coal mine, and buy precious time for emergency rescue and disaster relief.

The invention provides a method for tracing pollutants by combining water quality parameters with a three-dimensional fluorescence spectrum. The method comprises the following steps of water quality testing, fluorescence detection, pollution source type identification, and pollution source determination. The pollutant tracing method is combined with PMF model factor analysis results and the fluorescence intensity ratio of characteristic fluorescent components to quickly judge whether a water body environment is affected by human activities or not and judge the type of a water body pollution source at the same time; in addition, targeted screening of pollution sources is carried out by combining with relevant regional water quality monitoring distribution points with hydrological parameters; and finally, main pollution sources of a water body are defined by comparing test results of the corresponding pollution sources. The method has the advantages of simple operation, high accuracy, good reproducibility and the like; typical pollution sources in the water body environment can be quickly and effectively identified and theoretical guidance is provided for control and treatment of related pollution.

The invention discloses a method for rapidly identifying and determining the black and odor of an urban water body based on multi-source spectral characteristics. A three-dimensional fluorescence spectrum is used for analyzing concentrations of humic acid, albumin and oil black and odor precursors in the water body, and a UV-visible absorption spectrum is utilized for analyzing and obtaining waterquality parameters of turbidity, chroma and nitrate black and odor and other related parameters, and water quality comprehensive indexes of COD, BOD and TOC are obtained by using fluorescence spectrum and absorption spectrum fusion inversion; combining dissolved oxygen and water temperature parameters, a water body black and odor grade measurement algorithm model is established based on data driving through testing and training of different types of water bodies in a plurality of cities, and the rapid identification and determination of the black odor grade of the water body is achieved. Themethod solves the problems of 'single monitoring index, organic index missing and ammonia nitrogen sampling and off-line measuring' in a water body black and odor monitoring method specified in Guideto Regulation of Urban Black and Odor Water Bodies, has the advantages of no reagent, no need of sample pretreatment, rapid measurement and the like, and is a rapid and effective method for measuringthe black and odor of water bodies.

The invention relates to a method for quickly determining flow direction of groundwater polluted by organic matter, which mainly comprises the following steps of A, sample collection; B, sample pretreatment and fluorescence spectrum determination; C, three-dimensional fluorescence spectrum data processing; D, proportion calculation of area fluorescence volume of different kinds of fluorescent organic matter in total volume; E, similarity analysis of components of the fluorescent organic matter in groundwater samples in different places; and F, determination of the flow direction of the groundwater. The method has the advantages of little sample demand, high detection sensitivity, less workload, high detection speed and environment friendliness.

The invention relates to a method for identifying two flue-cured tobaccos with different odor types in Guizhou, belonging to the field of tobacco chemistry. The method disclosed by the invention adopts a three-dimensional fluorescence spectrum technology to perform clustering identification on the two different flue-cured tobaccos, namely fresh, sweet and aromas flue-cured tobaccos planted in the southwest of Guizhou province, Liupanshui and Bijieyu, and mellow, sweet and aromas flue-cured tobaccos planted in the southeast of Guizhou province, Tongren, Zunyi and the south of Guizhou province. Relative content difference information of chemical substances in the flue-cured tobaccos of different aromas types is obtained by measuring overall characteristic spectrums of chemical components in petroleum ether extracting solutions of tobacco leaves according to positions and intensities of characteristic spectrum peaks; and clustering analysis is carried out by statistical analysis software. The characteristic spectrum detection method is capable of identifying the aromas types of the two flue-cured tobaccos in Guizhou rapidly, simply, conveniently and accurately.

The invention discloses a percolate and a method for evaluating bioavailability of heavy metals in water polluted thereby. The method comprises the following main steps: collecting the percolate and/or water polluted by the percolate; extracting and purifying a sample; assaying and regulating the concentration of dissolved organic carbon (DOC) in the sample; assaying the heavy metal content of the regulated sample; performing three-dimensional fluorescence spectrum scanning on the regulated sample; performing parallel factor analysis on the scanning data; performing correlation analysis on the concentration of the heavy metals in the sample and a relative component concentration value obtained in the parallel factor analysis; and according to a result of the correlation analysis, evaluating the bioavailability of the heavy metals. The method is easy and safe to operate, short in required analysis time and low in analysis cost.

The invention discloses a structure and an application of a three-dimensional fluorescence standard spectrum library used for recognizing toxic-to-fish algae. The hemolytic activity and the change of the toxic-to-fish algae in different growing periods and under the control of environmental factors, and a chlorophyll three-dimensional fluorescence spectrum of the toxic-to-fish algae are researched and contrasted with a chlorophyll three-dimensional fluorescence spectrum of large sample nontoxic-to-fish algae, three-dimensional fluorescence spectrum analysis and recognition methods are screened, a fluorescence characteristic spectrum closely related to the toxic-to-fish algae and the hemolytic activity of the toxic-to-fish algae is extracted, and three-dimensional fluorescence standard spectrum libraries of the toxic-to-fish algae and the nontoxic-to-fish algae are screened by a clustering method; based on the three-dimensional fluorescence standard spectrum libraries, a Fisher discrimination function for recognizing the toxic-to-fish algae and a function for discriminating the degree of the hemolytic activity of the toxic-to-fish algae are established respectively. Discrimination results obtained by utilizing the discrimination functions are more stable, accuracy and reliable. The method provided by the invention realizes highly correct diagnosis and recognition functions for the toxic-to-fish algae in an in-place red tide water body and the hemolytic activity of the toxic-to-fish algae.

The invention discloses a sewage plant water-quality monitoring early-warning method and system. The method comprises the following steps that 1, sampling is conducted on the portion of sewage plant inflow water and the portion of effluent subjected to complete flow treatment of a sewage plant, and multiple inflow water samples and multiple corresponding effluent samples are obtained; 2, according to a three-dimensional fluorescence spectrogram of the inflow water samples and water quality indexes of the inflow water samples, a mapping relation between three-dimensional fluorescence spectrogram data and the water quality indexes of the inflow water samples is established; 3, the water quality indexes of the inflow water samples serve as input values, water quality indexes of the effluent samples serve as output values, and a BP neural network is established through training; 4, a three-dimensional fluorescence spectrogram of current inflow water is obtained; 5, the water quality index of the current inflow water is obtained through mapping relationship analysis according to the three-dimensional fluorescence spectrogram of the current inflow water, and the water quality index of the effluent corresponding to the inflow water is obtained through BP neural network prediction according to the water quality index of the current inflow water. By means of the sewage plant water-quality monitoring early-warning method and system, the anomaly of the effluent quality can be monitored in advance.

The invention discloses a method for determining the content of pigments in mixed pigment solutions by adopting three-dimensional fluorescence spectroscopy combined with the PARAFAC (parallel factor analysis) algorithm. According to the method, firstly, a correction sample set is formed by preparing multiple mixed pigment solutions according to different concentrations, then three-dimensional fluorescence spectra of the correction sample set and the background are measured, measured signals are pre-processed, a three-dimensional data matrix after pre-processing is analyzed with the PARAFAC algorithm, a correction model is established according to an analysis result, and the content of the pigments in unknown samples is predicted with the correction model. The method overcomes the defects of tedious process, time consumption of detection, higher detection cost and the like of a traditional chemical detection method, the concentration of each pigment in the to-be-detected samples can be determined rapidly, the method has the advantages of rapidness, safety, reliability, simple operation, lower cost and the like, technical support is provided for determination of synthetic edible pigments in food, and supervision of the food industry and protection of consumer interests are facilitated.

The invention discloses a rapid identification and quantitative analysis method for water pollution based on three-dimensional fluorescence spectrum, and belongs to the field of spectral analysis. The method comprises firstly arranging three-dimensional fluorescence spectrum parameters, performing rapid identification on polluted water and analyzing compositions in the polluted water, then preparing a to-be-detected liquid, arranging parameters, acquiring data and performing quantitative analysis. The method is rapid and simple for determination and high in sensitivity, is applicable to qualitative analysis and quantitative evaluation on sewage processing effects, helps to relatively well guide design, operation, management and control of a sewage processing technology, and also possesses extremely good reference value on water quality monitoring.