Method for correcting and calibrating three-dimensional fluorescence data of colored soluble organic matters

Abstract

The invention provides a method for correcting and calibrating three-dimensional fluorescence data of colored soluble organic matters. The method comprises the steps of setting measurement conditions such as three-dimensional fluorescence excitation, a wavelength generation range and a scanning speed; measuring a three-dimensional fluorescence spectrum of a sample; measuring a three-dimensional fluorescence spectrum of ultrapure water; performing Raman scattering correction by subtracting excitation-emission three-dimensional fluorescence spectrum of the ultrapure water from the excitation-emission three-dimensional fluorescence spectrum of the sample; assigning 0 to a triangular region of each three-dimensional fluorescence spectrum to perform Rayleigh scattering correction; performing calibration on the fluorescence intensity through the excitation wavelength of 350nm and the emission wavelength of 371-428nm of the ultrapure water; measuring an absorption spectrum of the sample to obtain the absorbency of the sample; performing inner filering effect correction on the three-dimensional fluorescence data according to the absorbency of the sample. According to the method disclosed by the invention, the influence of the inner filtering effect of the high-concentration sample on the fluorescence intensity can be eliminated without dilution of the sample.

Description

technical field [0001] The invention relates to the field of environmental science and monitoring technology, in particular to a three-dimensional fluorescence measurement of colored soluble organic matter in environmental samples and a data correction and calibration method. Background technique [0002] Colored soluble organic matter is an important chemical component in various environmental media on the surface and is widely distributed. More and more studies have shown that: it plays a very important role in the physical, chemical and biological processes of the surface ecosystem; important way. Therefore, colored soluble organic matter is not only one of the important contents in the research fields of biogeochemistry, ecology and environmental science, but also a basic theoretical issue in the evaluation, prediction and treatment of environmental pollution. Scientific issues of common concern to environmental legislation and management research. Colored soluble org...

AI Technical Summary

Problems solved by technology

However, due to the fact that the fluorescence of colored soluble organic compounds has no consistent standard for the emission and absorption of different fluorometers, and the intensity of the fluorometer lamp is affected by environmental conditions and service life, different fluorometers and the same fluorometer can be used in different environments. There are differences in the three-dimensional fluorescence instrument data of the same sample measured under the same conditions, and quantitative comparison cannot be performed

[0004] Although the measurement results of samples on different fluorometers are significantly different before calibration, and the instrument calibration factors provided by different instrument manufacturers are different, the overlap of the fluorescence spectra measured by the same sample after calibration is improved.

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