Method for determination of form of selenium in selenium-rich plant material

A technology of selenium-enriched plants and a determination method, which is applied in the field of determination of selenium form after pretreatment of selenium-enriched plant materials, can solve the problems of unclear form of organic selenium, cumbersome operation steps, poor repeatability, etc., and achieve thorough hydrolysis and use The effect of small quantity and low cost
CN102520104AInactive Publication Date: 2012-06-27SUZHOU SETEK
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU SETEK
Publication Date
2012-06-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for determination of the form of selenium in a selenium-rich plant material. The method provided by the invention is characterized by comprising the following steps of 1, a selenium-rich plant material pretreatment process comprising carrying out extraction of a crushed selenium-rich plant material sample by an extraction buffer Tris-HCl, after the extraction, adding cellulase into the extract, carrying out wall breaking of cells of the selenium-rich plant material sample, orderly adding effective amounts of protease K and protease XIV into the extract, and carrying out hydrolysis extraction to obtain a solution of a sample needing to be detected, and 2, carrying out determination processes respectively on a solution of selenium having a standard form and the solution of the sample needing to be detected by the combination of the hydride generation-atomic fluorescence spectroscopy technology and the high performance liquid chromatography. The method provided by the invention is simple, can be realized easily, adopts nontoxic reagents and does not produce pollution.
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Description

technical field

[0001] The invention relates to a method for determining the form of selenium in a selenium-rich plant material, in particular to a method for measuring the form of selenium in a selenium-rich plant material after pretreatment. Background technique

[0002] Selenium is one of the essential trace elements for the human body and a component of glutathione peroxidase (GSH-Px), which has the functions of anti-oxidation, anti-cancer, anti-aging, improving human immunity, and protecting cardiovascular and cerebrovascular ( Rotruck J T, Pope A L, Ganther H E, et al Selenium: biochemical role as a component of glutathione peroxidase. Science, 1973, 179, 588-590.). There are two-thirds of selenium-deficient areas in my country, and one-third of them are severely selenium-deficient areas. Selenium deficiency in humans and animals can cause Keshan disease, Kaschin-Beck disease, white muscle disease of animals, etc. In addition, some cancers, endemic thyroid disorders a...

Claims

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