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Method for characterizing humification degree of compost

A technology of humification and composting, applied in the direction of material excitation analysis, fluorescence/phosphorescence, etc., can solve the problems of compost humification degree errors, etc., and achieve high accuracy, high repeatability, and high repeatability

Inactive Publication Date: 2012-08-15
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Application Information

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Problems solved by technology

However, when the compost organic matter contains a large amount of complex structure substances, the degree of humification is high, and the fluorescence peak of the sample appears in the range of excitation wavelength greater than 254nm, there will be large errors in calculating the degree of humification of compost by this method

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  • Method for characterizing humification degree of compost
  • Method for characterizing humification degree of compost
  • Method for characterizing humification degree of compost

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Collect 8g samples of cow manure composting days 0, 7, 14, 21, 28 and 41 from a composting plant in Zhangjiakou, add 80ml of deionized water after grinding, shake at 150rmp for 16h to obtain the extract, centrifuge at 4°C and 10000rmp for 10min to obtain The centrifugate was passed through a 0.45 μm filter membrane to obtain water-soluble organic matter samples.

[0027] Measure the DOC of the water-soluble organic matter sample and adjust the solution to DOC=7.0mg / L, put it into a cuvette to measure the three-dimensional fluorescence spectrum, set the excitation wavelength to 200-440nm, set the emission wavelength to 280-550nm, and set the slit The width is set to 10nm, and the scanning speed is 1500nm / min. During the scanning process, a 290nm filter is used to remove secondary Rayleigh scattering. After the spectrum is scanned, the data is exported. One point is taken every 1nm of the emission wavelength, and a 266-line×25 Columns of 3D fluorescence spectrum matrix da...

Embodiment 2

[0030] Collect samples 10 of 0, 7, 14, 21 and 41 days of composting in Beijing Asuwei Domestic Garbage Composting Plant, add 100ml of deionized water after grinding, shake at 150rmp for 16h to obtain the extract, centrifuge at 10000rmp for 10min to obtain the centrifugate, and pass the centrifugate A 0.45 μm filter membrane was used to obtain samples of water-soluble organic matter.

[0031] Measure the organic carbon DOC content of the water-soluble organic matter sample and adjust it to DOC=6.0mg / L, put it into a cuvette to measure the three-dimensional fluorescence spectrum, set the excitation wavelength to 200-440nm, and set the emission wavelength to 280-550nm. Slit width: the excitation wavelength is 10nm, the emission wavelength is 10nm, and the scanning speed is 1500nm / min. During the scanning process, a 290nm filter is used to remove secondary Rayleigh scattering, and the data is exported after spectral scanning. One point is taken for every 1nm of the emission wavelen...

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Abstract

A method for characterizing humification degree of compost includes steps of taking a compost sample, adding water in the compost sample and shaking to obtain extract liquor, and taking a supernate filter membrane after centrifugation to prepare water-soluble organic matter sample solution of the compost; performing three-dimensional fluorescence spectrometry for the water-soluble organic matter sample solution of the compost and exporting data, and taking points in identical intervals one by one according to transmitting wavelength so as to obtain three-dimensional fluorescence spectrum matrix data; assigning the data with excitation wavelength larger than or equal to the transmitting wavelength in the three-dimensional fluorescence spectrum matrix data as 0, and adducting data in a range that the excitation wavelength is larger than or equal to 200nm and smaller than or equal to 440nm and the transmitting wavelength is larger than or equal to 280nm and smaller than 380nm so as to obtain the sum P of total fluorescence intensities of albuminoid substances; and adducting data in a range that the excitation wavelength is larger than or equal to 200nm and smaller than or equal to 440nm and the transmitting wavelength is larger than or equal to 380nm and smaller than or equal to 550nm so as to obtain the sum H of total fluorescence intensities of humic-like substances. The humification degree of the compost sample can be compared and evaluated according to an H / P value.

Description

technical field [0001] The invention belongs to the field of solid organic waste treatment and resource utilization, and in particular relates to a method for characterizing the degree of humification of compost samples through three-dimensional fluorescence spectrum. Background technique [0002] Composting can realize the harmless treatment and resource utilization of organic solid waste, and obtain the organic fertilizer needed for soil improvement. In the composting process, organic matter degrades on the one hand, and humifies on the other hand. The degree of humification of organic waste directly affects the quality of compost products and the potential for environmental nutrient release. Compost structures with a high degree of humification Stable, it can release the fixed nutrient elements stably for a long time, and it will not degrade and produce malodorous gas that pollutes the surrounding environment. [0003] The most commonly used techniques for evaluating the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 何小松席北斗姜永海白顺果安达杨昱
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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