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Method for identifying two flue-cured tobaccos with different odor types in Guizhou

A technology of flue-cured tobacco and aroma type, applied in the field of tobacco chemistry, can solve problems such as difficulty in giving objective, quantitative and digital descriptions, etc., and achieve the effects of objective discrimination results, fast spectral measurement, and simple sample processing methods.

Inactive Publication Date: 2013-07-24
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] At present, the traditional discrimination of tobacco flavor type and aroma style is carried out by sampling and manual evaluation. It is carried out through aroma profile analysis, and it is difficult to give an objective, quantitative and digital description.

Method used

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  • Method for identifying two flue-cured tobaccos with different odor types in Guizhou
  • Method for identifying two flue-cured tobaccos with different odor types in Guizhou
  • Method for identifying two flue-cured tobaccos with different odor types in Guizhou

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Discrimination of Fluorescence Intensity Fractions in Fluorescence Spectroscopy for Two Types of Flue-cured Tobacco with Different Aromas in Guizhou

[0034] The grade of collecting 7 tobacco planting areas in Guizhou is C 3 F (Zhongju 3) 125 samples of flue-cured tobacco leaves, each with a sampling volume of 1kg, were dried in an oven at 40°C for 6 hours, crushed, and passed through a 40-mesh sieve. Accurately weigh 1g of smoke sample, infiltrate with 30ml of chromatographically pure petroleum ether, vibrate for 2 hours and then let it stand for 24 hours. Take 0.5 mL of the supernatant treated by the above sample processing method, dilute it 20 times with chromatographically pure petroleum ether, and transfer it to a polished non-fluorescent quartz cuvette with a thickness of 1 cm on four sides. The excitation wavelength is 200-500 nm, and the emission wavelength is 200 nm. In the range of ~600 nm, the wavelength is incremented by 2.0 nm, the exit and entr...

Embodiment 2

[0036] Example 2: Discrimination of Fluorescence Intensity Ratio in Fluorescence Spectrometry Two Types of Flue-cured Tobacco with Different Aromas in Guizhou

[0037] Sample collection, processing and spectral measurement methods are the same as in Example 1. Calculate the fluorescence intensity ratio R of the three characteristic fluorescence peaks 1 , R 2 , R 3 . where R 1 =FI / FIII,R 2 = FII / FIII,R 3 = FIII / FI. The three-dimensional fluorescence intensity ratio data of each smoke sample were obtained (Table 2). Using SPSS17.0 data statistical analysis software, in the "variable view" define "sample number, R 1 , R 2 , R 3 "4 variables, of which the "sample number" is a string type, and the other three are numeric types. Input the sample numbers of different tobacco growing areas and the fluorescence intensity ratio data of tobacco leaf petroleum ether extracts in each area into the "Data View", select "Analysis"→"Classification"→"System Cluster Analysis" operatio...

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Abstract

The invention relates to a method for identifying two flue-cured tobaccos with different odor types in Guizhou, belonging to the field of tobacco chemistry. The method disclosed by the invention adopts a three-dimensional fluorescence spectrum technology to perform clustering identification on the two different flue-cured tobaccos, namely fresh, sweet and aromas flue-cured tobaccos planted in the southwest of Guizhou province, Liupanshui and Bijieyu, and mellow, sweet and aromas flue-cured tobaccos planted in the southeast of Guizhou province, Tongren, Zunyi and the south of Guizhou province. Relative content difference information of chemical substances in the flue-cured tobaccos of different aromas types is obtained by measuring overall characteristic spectrums of chemical components in petroleum ether extracting solutions of tobacco leaves according to positions and intensities of characteristic spectrum peaks; and clustering analysis is carried out by statistical analysis software. The characteristic spectrum detection method is capable of identifying the aromas types of the two flue-cured tobaccos in Guizhou rapidly, simply, conveniently and accurately.

Description

Technical field [0001] The present invention is a tobacco chemistry field. Specifically, it is a method of identifying two different types of fragrant smoke in Guizhou, that is, the method of identifying the two different types of fragrant smoke in the "sweet fragrance" type and "mellow sweet fragrance" type. Background technique [0002] Tobacco flavor is the result of the common effect of a variety of aroma ingredients, and it is an important indicator of the quality of tobacco and its products.The quality and fragrance of tobacco leaves are directly related to the type and content of the incense substances.There are many types of substances that form the flavor of tobacco, the content of the ingredients is extremely small, and there are extremely complicated interactions between various fragrance components.The fragrance and aroma quality of tobacco leaves in different planting areas are related to the type, variety, ecological environment, and cultivation conditions. The comp...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 冉霞牟兰刘仁祥曾晞
Owner GUIZHOU UNIV
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