59 results about "Tobacco chemistry" patented technology

The invention discloses a of a phenylpropanoid compound showing as the first formula (Please see the formula in the instruction) and a preparation method and application thereof and belongs to the technical field of tobacco chemistry. Tobacco serves as a raw material, extraction is conducted with a high-concentration methanol aqueous solution or a high-concentration ethanol aqueous solution or a high-concentration acetone aqueous solution as an extraction solvent, and the extraction solutions are merged, filtered, and decompressed condensed to form extract; the extract is packed by the adoption of a silica gel to be conducted silica gel column chromatography; a mixing solvent of chloroform-acetone serves as an eluent to conduct gradient elution, and TLC monitors and merges same parts and condenses; separation and purification by use of high-pressure liquid chromatography are further conducted on the part obtained by eluting, collecting and condensing by the mixing solvent of the chloroform-acetone with a volume matching of 6:4, and the phenylpropanoid compound is obtained. The activity test indicates that the compound has a good inhibiting effect on rotavirus. The compound is simple in structure, good in activity and capable of being used as a guiding compound for resisting the rotavirus.

The invention discloses a method for detecting alkaloids and nitrosamines in tobaccos simultaneously, and belongs to the technical field of tobacco chemical composition detection. The method comprises the working procedures of: sample extraction, purification, analysis and quantification. Firstly an internal standard solution and ultrapure water are added into tobacco leaf powder, extract liquor passes through a filter film after ultrasonic extraction, then methanol is added, protein is removed through centrifugation after shaking, and supernatant is subjected to liquid chromatography-mass spectrometry. After standard solutions of compounds to be detected with different concentration gradients are prepared by adopting a mixing preparation method, and the liquid chromatography-mass spectrometry is carried out, standard curves of instrument responses of the compounds to the actual concentrations of the solutions are drawn, curve equations are fitted, and the detection concentrations are calculated according to measured values of the instrument responses of the compounds to be detected and converted to the actual concentrations of the compounds to be detected in tobacco samples. The detection method provided by the invention can be used for carrying out the qualitative and quantitative analysis on alkaloids and nitrosamines in the tobacco samples simultaneously, and is easy and convenient to operate, good in reproducibility, high in analytic sensitivity and accurate in quantification.

The invention discloses a benzolactone compound shown in the formula (I) and its preparation method and use, and belongs to the technical field of tobacco chemistry. The preparation method comprises carrying out extraction on tobacco leaf as a raw material by a high concentration methanol aqueous solution, a high concentration ethanol aqueous solution or a high concentration acetone aqueous solution as an extraction solvent, merging the extract, carrying out filtration, carrying out reduced pressure condensation to obtain extract, carrying out silica gel column chromatography on the extract by a silica gel dry-packaged column, carrying out gradient elution by a chloroform-acetone mixed solvent as an eluant, carrying out TLC monitoring, merging the same parts, carrying out condensation, and by high pressure liquid chromatography, further separating and purifying the part subjected to elution based on the chloroform-acetone mixed solvent with a volume ratio of 7: 3, collection and condensation so that the benzolactone compound is obtained. A cigarette addition experiment proves that the benzolactone compound can make cigarette smell soft, improve cigarette smell fineness and improve oral cavity moistening feeling.

The invention relates to a method for identifying two flue-cured tobaccos with different odor types in Guizhou, belonging to the field of tobacco chemistry. The method disclosed by the invention adopts a three-dimensional fluorescence spectrum technology to perform clustering identification on the two different flue-cured tobaccos, namely fresh, sweet and aromas flue-cured tobaccos planted in the southwest of Guizhou province, Liupanshui and Bijieyu, and mellow, sweet and aromas flue-cured tobaccos planted in the southeast of Guizhou province, Tongren, Zunyi and the south of Guizhou province. Relative content difference information of chemical substances in the flue-cured tobaccos of different aromas types is obtained by measuring overall characteristic spectrums of chemical components in petroleum ether extracting solutions of tobacco leaves according to positions and intensities of characteristic spectrum peaks; and clustering analysis is carried out by statistical analysis software. The characteristic spectrum detection method is capable of identifying the aromas types of the two flue-cured tobaccos in Guizhou rapidly, simply, conveniently and accurately.

The invention discloses a tobacco chemical value quantifying method based on near-infrared spectrum wave number K-means clustering, comprising the following steps: establishing a training set and a test set, and collecting near-infrared spectrum and target component content of all tobacco samples in the training set; clustering the wave number of the near-infrared spectrum of each tobacco sample in the training set through K-means clustering; after each clustering, using PLS to establish a relationship model between each subclass spectral band and the target component content, and calculating the root mean square error for cross validation of each relationship model; taking the number of clustering with the minimum sum of the root mean square error for cross validation corresponding to the relationship models as the optimal clustering number, and performing weighted summation on the relationship models corresponding to the optimal clustering number to obtain a full-spectrum model; and collecting near-infrared spectrum of each tobacco sample in the test set, and obtaining the target component content of each tobacco sample in the test set on the basis of the full-spectrum model. Compared with the existing PLS method, the method of the invention can significantly reduce the prediction error of a model.

The invention discloses a determination method of volatile N-nitrosamine in tobacco, tobacco products and smoke and belongs to the technical field of tobacco chemical inspection. The determination method comprises the steps that graphitized carbon black, N-propyl ethylenediamine bonded silica gel, anhydrous magnesium sulfate and a sodium chloride adsorbing agent are added into a sample extracting solution, centrifugation and filtration are carried out after dispersive solid-phase extraction, and a to-be-tested solution is obtained; the to-be-tested solution is taken for gas chromatography-tandem mass spectrum analysis, the content of volatile N-nitrosamine in the to-be-tested solution is obtained with reference to a standard curve, and the content of volatile N-nitrosamine in a sample is obtained through calculation. The method is high in detection sensitivity, good in accuracy and repeatability and easy, fast and efficient to operate, the relative recovery rate is between 82.4% and 112.4%, and the intra-day precision and inter-day precision are smaller than 9.1% and 8.2% respectively.

The invention belongs to the field of the chemical study of nicotiana tobacum, and particularly relates to a furan flavonoid compound separated from the nicotiana tobacum and application of the furan flavonoid compound to prevention and control of tobacco mosaic virus. According to the furan flavonoid compound, stems and roots of the nicotiana tobacum, which serve as raw materials are extracted and separated to obtain a compound 3,4'-dimethoxy-5-hydroxyl-7,8-(2''-ethyl furan)-flavone, wherein a structural formula of the compound is shown in the specification. The furan flavonoid compound has the advantages of environment friendliness, low cost, good effect of resisting the tobacco mosaic virus and the like, does not harm human and livestock and can be used as a novel plant virus prevention and control agent for tobacco mosaic virus.

The invention relates to a nicotine-oxalate compound crystal and application thereof, and belongs to the technical field of tobacco chemistry. The molecular formula of the crystal is 2(C10H15N2).C2O4.2.5(C2H2O4). The crystal belongs to a monoclinic system, the space group is C2, and the cell parameters are as follows: a = 10.8406 (15) angstroms, b = 9.8946 (9) angstroms, c = 33.102 (5) angstroms,alpha = 90.00 degrees, beta = 93.481 (13) degrees, gamma = 90.00 degrees and V = 3544.1 (8) angstroms<3>. The nicotine-oxalate compound crystal provided by the invention provides a new introduction form for nicotine of related products such as electronic cigarettes, heat-not-burn cigarettes and the like; when the crystal is used, the taste can be improved, the nicotine delivery process is influenced, and the physiological satisfaction can be continuously provided.

The invention relates to polyphenol active compound in aromatic tobacco and preparation method and application thereof, belongs to the field of tobacco chemical research, and more particularly relates to polyphenol active compound obtained by separating aromatic tobacco, and application of the polyphenol active compound to controlling tobacco mosaic virus. Entire tobacco plants are subjected to extraction by 95% methanol, and are subjected to silica column chromatography and coarse segmentation prior to separation by preparative chromatography so as to obtain the polyphenol active compound (I). The polyphenol active compound has the advantages of environmental friendliness, low cost, safety to people and livestock, high tobacco mosaic virus resistance and the like, and can be used as novel plant virus controlling agent for tobacco mosaic virus.

The invention relates to an extraction and determination method of special N-nitrosamine of tobacco in tobacco or a tobacco product based on hydrophobic ionic exchange solid phase extraction and belongs to the technical field of tobacco chemical examination. The extraction method, provided by the invention comprises the following steps of: adding the tobacco or the tobacco product into an ammonium acetate solution, the concentration of which is 5-100mmol/L, to be extracted; passing the obtained coarse extraction liquid through an activated HiCapt MCX solid extraction column; and then leaching and eluting the liquid to obtain the N-nitrosamine. The extraction method of special N-nitrosamine of tobacco in tobacco or the tobacco product based on hydrophobic ionic exchange solid phase extraction provided by the invention can effectively remove impurities and interferents, shorten the treatment time and improve the accuracy and precision of the determination method. The relative recovery rate of the method is between 92.2% and 108.9%. The within-day precision and the between-day precision are separately smaller than 4.6% and 4.2%.

The invention relates to a prediction method of hydrocyanic acid release amount in main stream smoke of flue-cured tobacco leaves. The method comprises the steps of subjecting tobacco leaves to be cured firstly to sample pre-processing such as manual piece tearing, stalk removing and tobacco cutting; detecting 6 chemical components (water, chlorine, malonic acid, volatile acid, potassium and total nitrogen) of a sample to be detected; calculating network values of 11 nodes of a hidden layer according to the detection results of the 6 chemical components and coefficients of input layers of a model; converting the network values of the 11 nodes of the hidden layer to output values of the 11 nodes of the hidden layer; and calculating to obtain a predicted value of the hydrocyanic acid release amount in the smoke according to the output values of the 11 nodes of the hidden layer and coefficients of output layers of the model. According to the method, constant detection of the 6 tobacco chemical components of the sample to be detected is performed, the hydrocyanic acid release amount in the smoke can be predicted through the model, and the possible hydrocyanic acid accumulated content in cigarette finished products produced in future can be predicted effectively according to raw materials of the flue-cured tobacco leaves, so that raw material choosing in a producing process is guided.

The invention relates to a method for predicting the release amount of crotonaldehyde in mainstream smoke of flue-cured tobaccos. The method comprises the following steps of: performing sample pretreatment on the flue-cured tobaccos to be tested through three steps of manual tearing, stem removal, shredding; detecting five chemical components comprising palmitic acid, tobacco moisture, total polyphenol, total nitrogen and protein of a sample to be tested; calculating network values of eight nodes of a hidden layer by combining the measurement results of the five chemical components with the coefficients of each input layer of a model; converting the network values of the eight nodes of the hidden layer into the output values of the eight nodes of the hidden layer; and calculating to obtain the predicted value of the release amount of the tobacco crotonaldehyde by combining the output values of the eight nodes of the hidden layer with the coefficients of the output layers of the model. According to the method, the five chemical component constants of the tobacco in the sample to be tested are detected, prediction calculation is performed by matching the established model structure with the model coefficients, and the crotonaldehyde accumulation content to be formed in the future can be effectively predicted through the flue-cured tobacco raw materials, so that raw material selection in the production process is guided.

The invention belongs to the technical field of tobacco chemical component analysis, and particularly relates to an HPLC-DAD segmented detection method for simultaneously determining 14 polyphenol compounds in tobacco. According to the method, a potassium dihydrogen phosphate aqueous solution and methanol are used as mobile phases; gradient elution is carried out according to a specific proportion; and 14 polyphenol compounds in the tobacco can be simultaneously determined, so that the segmented wavelength is further limited, the signal intensity of the polyphenol compounds can be remarkably improved, the sensitivity is high, the peak pattern of a target peak is good, the separation degree is high, the determination of low-content polyphenol compounds in the tobacco is facilitated, and theinterference of other impurities is reduced; and it is proved by methodological investigation that the method provided by the invention has high precision, good reproducibility and high accuracy, and can be used in tobacco quality control and sensory characteristic analysis.

The invention relates to a method for preparing nicotine sulfate, and belongs to the technical field of tobacco chemistry. According to the method, an aquaporin membrane and a polyamide membrane are used as forward osmosis concentration membranes, an inorganic salt is used as an absorbing agent, and a low-concentration nicotine distillate is subjected to forward osmosis concentration until the trapped fluid nicotine concentration is 5%-20%; 5%-40% sulfuric acid which is slightly excessive in chemical calculation is added into a trapped fluid to obtain a nicotine sulfate solution with relatively high concentration, and then evaporating and concentrating are carried out to obtain a 40%-60% nicotine sulfate product. According to the method, the concentration of the reactant nicotine during nicotine sulfate generation is greatly improved by adopting forward osmosis concentration, so that the concentration of the reaction product nicotine sulfate is improved, the evaporation capacity of water during preparation of a final product by subsequent evaporation and concentration is reduced, and the evaporation energy consumption is greatly reduced; by utilizing the characteristic of poor interception efficiency of forward osmosis concentration on ammonia, the ammonia content in the trapped liquid is greatly reduced, so that the ammonium salt content in a final product is greatly reduced,and the purity of nicotine sulfate is greatly improved.

The invention relates to a sterol compound in tobacco and a preparation method and application of the sterol compound and belongs to the technical field of tobacco chemicals. The sterol compound is prepared from air-cured tobacco in Yunan through separation, is named as Tobsterol-A, has a molecular formula of C22H34O3, is white powder and has a structural formula (I) shown in the description. Thepreparation method of the sterol compound comprises the following steps: performing extract extraction, performing column chromatography on silica gel, and performing separation and purification withhigh pressure liquid chromatography. The compound provided by the invention is simple in preparation process and easy in industrial production, and has conditions of large-scale popularization and application. Meanwhile, the compound provided by the invention has good tobacco black shank prevention activity, the diameter of an inhibition zone of the sterol compound provided by the invention is 14.5+/-1.4mm, and the compound has a tobacco phytophthora inhibition effect which is remarkably prior to that of positive control, namely agricultural chloramphenicol. In addition, the compound providedby the invention has a remarkable tobacco black shank prevention and treatment effect and is easy to popularize and use.

The invention relates to a phase chromatography tandem mass spectrum measuring method for 4-(methyl nitrosamino)-1-(3-pyridyl)-1-butanone(NNK) and N-nitrosonornicotine (NNN) in tobacco, and belongs tothe technical field of tobacco chemistry analysis. A methanol solution containing 0.5% of acetic acid is used for extracting NNK and NNN in a tobacco sample, and after the extracting solution is filtered, UPC2-MS/MS is used for analyzing and quantifying the concentration of NNK and NNN in the extracting solution. The sample treating process is simple and quick, and batched strict work analysis isconvenient. When the phase chromatography tandem mass spectrum is used for detection, the used main moving phase is supercutical fluid CO2, a small amount of methyl alcohol is used as a cosolvent, and the environmentally friendly advantage is achieved. According to the method, the detection limit of NNK is 0.05 ng/mL, the quantitation limit is 0.18 ng/mL, the detection limit of NNN is 0.04 ng/mL,and the quantitation limit is 0.12 ng/mL. The method is high in sensitivity and good in repeatability and is applied to synchronous measurement of NNK and NNN in the tobacco sample.

The invention relates to a preparation method of a tobacco powder monitoring sample suitable for chemical conventional component inspection of tobacco. The preparation method is characterized by comprising the following steps: crushing, uniformly mixing, sub-packaging and sealing, inactivating, carrying out a uniformity experiment, valuing and inspecting stability. The preparation method has the advantages that the manufactured sample is good in uniformity, the constant value of related indexes is accurate, the storage life of the sample is long, the stability is good, the quality control of the chemical index test result can be effectively realized and the accuracy and the objectivity of the inspection result are ensured.

The invention discloses a method for carrying out chemical bud inhibition on tobaccos through bud suppression swabs. The method comprises the following steps: pesticide preparation: fully and uniformly mixing butralin emulsifiable concentrate, water and ethanol; or fully and uniformly mixing flumetidine emulsifiable concentrate, water and ethanol; preparing wooden toothpicks, soaking the wooden toothpicks which are dried by subsequent use in prepared pesticide, drying the wooden toothpicks in the shade after the toothpicks fully absorb the pesticide, and properly storing the toothpicks; enabling a vertex to be slightly higher than a top leaf; after tobacco plants are topped, successively germinating axillary buds from top to bottom and grow into tobacco branches, so that buds are timely smeared after topping, unnecessary nutrient consumption is reduced, and normal leaf growth is promoted; and after the buds are smeared, inserting the prepared bud suppression swabs into axillary positions of tobacco plant leaves in time, and inserting the bud suppression swabs according to the sequence from top to bottom, wherein the insertion depth is more than half of the length of the bud suppression swabs. The method disclosed by the invention has the beneficial effects that the regional precise pesticide application of tobacco chemical bud inhibition is realized, the method is simple to operate and practical, the labor intensity of the bud inhibition link of a tobacco grower after the topping of the tobacco is reduced, and the personnel labor is also reduced.

The invention relates to a determination method of correlation between functions of natural essence and flavor used in cigarettes and volatile components and belongs to the technical field of tobacco chemical industry and applications. In the determination method provided by the invention, the classification of volatile chemical components and the statistical analysis of regression coefficients are introduced. The method comprises the steps of: positioning of functional properties of the natural essence and flavor used in the cigarettes, detection of the volatile components and component classification, and research on the correlation between the functions and the components. Instruments and equipment used in the determination method are all purchased from the market, and the determination method has the beneficial effects that through the combination of the smoking positioning with the detection and the classification of the volatile components, a regression curve is built through a regress function of an MATLAB (Matrix Laboratory), and the functional properties of the unknown natural essence and flavor used in the cigarettes are determined through mathematical calculation so as to open up a new application field for the tobacco chemistry and application technologies, and provide a new method and train of thought for the chemical research of aa cigarette mass spectrometry.