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Sterol compound in tobacco and preparation method and application of sterol compound

A technology of compounds and sterols, applied in the field of sterols in Yunnan local air-cured tobacco and its preparation, can solve problems such as no reports.

Active Publication Date: 2019-12-20
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on the above considerations, we studied the chemical components of the dried whole plant of Yunnan sun-cured tobacco produced in Yunnan, and isolated a new sterol compound from it, which has not been reported so far

Method used

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  • Sterol compound in tobacco and preparation method and application of sterol compound
  • Sterol compound in tobacco and preparation method and application of sterol compound
  • Sterol compound in tobacco and preparation method and application of sterol compound

Examples

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Comparison scheme
Effect test

Embodiment 1

[0054] The preparation of embodiment 1-compound

[0055] The preparation method of a kind of sterol compound in tobacco, is to take the air-dried tobacco in Yunnan as raw material (from Honghe, Yunnan), and it is prepared through extraction of extract, silica gel column chromatography and high-pressure liquid chromatography separation and purification steps, specifically comprising the following steps :

[0056] A. Extraction of extract: crush the air-cured tobacco from Yunnan local area to 30-50 meshes, use 3 times the weight of Yunnan local air-dried tobacco to extract ultrasonically with 60% acetone for 3 times, each time for 30 minutes, and concentrate the combined extracts to obtain Extract a;

[0057] B. Silica gel column chromatography: the extract a obtained in step A is first dissolved in pure methanol with a weight ratio of 1.5 times of extract a, and then mixed with 80 mesh silica gel with 0.8 times the weight of extract a. At the same time, use extract a 300-mesh...

Embodiment 2

[0061] The preparation of embodiment 2-compound

[0062] The preparation method of a kind of sterol compound in tobacco, is to take the air-dried tobacco in Yunnan as raw material (from Honghe, Yunnan), and it is prepared through extraction of extract, silica gel column chromatography and high-pressure liquid chromatography separation and purification steps, specifically comprising the following steps :

[0063] A. Extraction: crush the air-cured tobacco from Yunnan to 30-50 meshes, use 10 times the weight of air-cured tobacco in Yunnan to extract ultrasonically with 90% acetone for 5 times, each time for 60 minutes, and concentrate the combined extracts to obtain Extract a;

[0064] B. Silica gel column chromatography: the extract a obtained in step A is first dissolved in pure ethanol with a weight ratio of 3 times of extract a, and then mixed with 100 mesh silica gel with 01.5 times the weight of extract a. At the same time, use extract a 160-mesh silica gel dry-packed wi...

Embodiment 3

[0068] The preparation of embodiment 3-compound

[0069] The preparation method of a kind of sterol compound in tobacco, is to take Yunnan local air-dried tobacco as raw material (from Yuanjiang, Yunnan), and prepare through extraction of extract, silica gel column chromatography and high-pressure liquid chromatography separation and purification steps, specifically comprising the following steps :

[0070] A. Extraction: crush 5.8kg of Yunnan local air-cured tobacco to 30-50 meshes, use 5 times the weight of Yunnan local air-cured tobacco for 75% acetone ultrasonic extraction for 3 times, each time for 50 minutes, and combine the extracts Concentrate to obtain flowable viscous extract a 427g;

[0071] B. Silica gel column chromatography: first dissolve the extract a obtained in step A with pure methanol with a weight ratio of 1.6 times of extract a, then mix the sample with 90 mesh silica gel with 1.2 times of extract a weight, and at the same time, use extract a 180-mesh s...

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Abstract

The invention relates to a sterol compound in tobacco and a preparation method and application of the sterol compound and belongs to the technical field of tobacco chemicals. The sterol compound is prepared from air-cured tobacco in Yunan through separation, is named as Tobsterol-A, has a molecular formula of C22H34O3, is white powder and has a structural formula (I) shown in the description. Thepreparation method of the sterol compound comprises the following steps: performing extract extraction, performing column chromatography on silica gel, and performing separation and purification withhigh pressure liquid chromatography. The compound provided by the invention is simple in preparation process and easy in industrial production, and has conditions of large-scale popularization and application. Meanwhile, the compound provided by the invention has good tobacco black shank prevention activity, the diameter of an inhibition zone of the sterol compound provided by the invention is 14.5+ / -1.4mm, and the compound has a tobacco phytophthora inhibition effect which is remarkably prior to that of positive control, namely agricultural chloramphenicol. In addition, the compound providedby the invention has a remarkable tobacco black shank prevention and treatment effect and is easy to popularize and use.

Description

technical field [0001] The invention belongs to the technical field of tobacco chemistry, and specifically relates to a sterol compound in tobacco and its preparation method and application, in particular to a sterol compound in Yunnan local air-cured tobacco and its preparation method and application. Background technique [0002] Sun-cured tobacco in Yunnan is a unique variant of tobacco in Yunnan. Tobacco entered Yunnan from the end of Wanli in Ming Dynasty, and became a common local crop in Qianlong period of Qing Dynasty. Yunnan has good natural conditions. Warm valleys, cool mid-levels, and mountainous areas are all suitable for the growth of tobacco. Moreover, due to the diverse climate and geographical environment in Yunnan, tobacco has formed many air-cured tobacco varieties with local characteristics during the cultivation process for many years, among which the famous ones are: Luoping Badahe tobacco, Shizong Wuluohe tobacco, Huize Leye Yan, Mengzi Xin'an smoke, ...

Claims

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Application Information

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IPC IPC(8): C07D307/93A01N43/12A01P3/00
CPCC07D307/93A01N43/12C07B2200/07
Inventor 曾婉俐杨光宇高茜向海英孔维松李雪梅李晶黄海涛王晋许永宋春满刘欣胡秋芬
Owner CHINA TOBACCO YUNNAN IND
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