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Application of labdanum compound in control of crop bacterial wilt

A technology of Lai Baidang and compounds, applied in the field of biological control of crop diseases, can solve problems such as unsatisfactory control effects of bacterial wilt, food safety, environmental pollution, etc., and achieve rich content, low production cost, and rich sources of raw materials Effect

Inactive Publication Date: 2020-07-10
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problems of unsatisfactory control effect, environmental pollution and food safety of existing crop bacterial wilt control technology, the present invention provides a method for preventing and controlling crop bacterial wilt by using Labatin-like compounds

Method used

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  • Application of labdanum compound in control of crop bacterial wilt
  • Application of labdanum compound in control of crop bacterial wilt
  • Application of labdanum compound in control of crop bacterial wilt

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Extraction of Layburn-like compounds from tobacco

[0022] (1) Raw material selection: select the sun-cured variety Longleaf Willow, with a planting area of ​​667m 2 , Picking fireworks and tobacco branches during the flowering period as raw materials for extraction.

[0023] (2) Extraction: The above-mentioned raw materials are directly extracted in a fresh state without being dried and pulverized. The solvent is absolute ethanol, and the amount of solvent used is 1.2 times the volume of the raw material. All the raw materials are continuously extracted twice, and the extraction time is 2 minutes each time. The extraction liquid is recycled. solvent, and all extracts were combined and filtered to obtain 120L extract.

[0024] (3) Concentration: the extract was concentrated to 20 L under reduced pressure at 50° C., and the solvent was recovered.

[0025] (4) Purification: The concentrated solution was left to stand at 2°C for 6 hours, and flocculent precipitates a...

Embodiment 2

[0054] 1. Extraction of Layburn-like compounds from tobacco

[0055] Selected cigar variety Oosikappal with 667m acreage 2 , Picking fireworks and tobacco branches during the flowering period as raw materials for extraction. Extraction method is the same as implementation example 1. Obtain 295g of tobacco rasbutane compound extract.

[0056] 2. Determination of abitol content

[0057] The detection method is the same as that in Example 1. After detection, the content of abbitol in the obtained extract is 9.8%.

[0058] 3. Preparation of Extract Emulsifier

[0059] Prepare the oil phase: prepare the emulsifier oil phase according to the following ratio: 0.5% abitol (weighed according to the content of the target compound in the extract), 20% solvent 150#, 10% emulsifier 3326, mix well;

[0060] Prepare the water phase: prepare the emulsifier water phase according to the following ratio: 10% ethylene glycol, 50% water, mix well;

[0061] Preparation of emulsion in water: t...

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Abstract

The invention belongs to the field of biological control of crop diseases, and provides application of cis-abienol in control of bacterial wilt and application of a tobacco labdanum compound in control of bacterial wilt directed at the problems of unsatisfactory control effect, environmental pollution and food safety in existing crop bacterial wilt control technology. According to the invention, bacterial wilt is controlled by improving the resistance of crops, and compared with currently reported biological source preparations, the labdanum compound has the characteristics of low effective concentration, high control effect, no toxicity, durability and safety.

Description

technical field [0001] The invention belongs to the field of biological control of crop diseases, and in particular relates to the application of resemblance compounds in the control of crop bacterial wilt. Background technique [0002] Crop bacterial wilt is a devastating soil-borne bacterial disease caused by Ralstonia solanacearum, commonly known as "crop febrile disease". The pathogen has a complex strain and a wide range of hosts. In addition to harming a variety of Solanaceae tobacco, tomato, eggplant, pepper, potato and other crops, it can also infect nearly 450 species of more than 50 families including peanuts, mulberry trees, and olives. plant. The prevention and control of bacterial wilt is the focus and difficulty of research in the field of agricultural science at home and abroad. Due to the lack of resistant varieties in production, although the current control is mainly based on chemical control, the control effect of the currently used chemical agents (such...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N31/06C07C29/76C07C35/36A01P21/00A01P3/00
CPCA01N31/06C07C29/76C07C2602/10C07C35/36
Inventor 杜咏梅张忠锋侯小东刘艳华付秋娟张鹏袁晓龙闫宁绪扩
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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