52results about How to "Good activity against tobacco mosaic virus" patented technology

The invention discloses a chromone derivative extracted from Cassia leschenaultiana as well as a preparation method and application of the chromone derivative. The chromone derivative is separated from complete Cassia leschenaultiana, the molecular formula of the chromone derivative is C18H20O3, and a compound of the chromone derivative is 6-(2,2-dimethyl-2-H-chromene)-2-isopropyl-8-methyl-4H-chromen-4-one. The preparation method of the chromone derivative comprises the following steps: taking the complete Cassia leschenaultiana as a raw material, smashing the complete Cassia leschenaultiana,extracting the smashed complete Cassia leschenaultiana and performing slica column chromatography and HPLC separation. Through a test for biological activity of the compound, the compound has a good effect of inhibiting a tobacco mosaic virus. The compound is easy to separate, is good in activity and can serve as a guiding compound for resisting the tobacco mosaic virus.

The invention discloses a chromone derivative, a preparation method and application thereof. The chromone derivative is separated from a cassia leschenaultiana whole plant, the molecular formula is C18H20O4, and the compound is named as: 6-(2, 2-dimethyl-2H-chromene)-2-isopropyl-8-methoxy-4H-chromen-4-one. The preparation method of the chromone derivative includes: taking a cassia leschenaultianawhole plant as the raw material, and conducting crushing, extraction, silica gel column chromatography and high pressure liquid chromatography separation steps. Biological activity test indicates thatthe compound provided by the invention has a good inhibiting effect on tobacco mosaic virus. The compound provided by the invention is easily separable, has good activity, and can be used as a leading compound against tobacco mosaic virus.

The invention provides an application of D-amino acid and D-amino acid derivatives to resistance of TMV (tobacco mosaic virus). The structural formula of the D-amino acid derivatives is shown as general formula I in the description, the anti-TMV activity of the D-amino acid and the D-amino acid derivatives is equivalent to or better than that of the commercialized variety virazole, further, the D-amino acid and the D-amino acid derivatives are not absorbed by a human body and are safer, and the D-amino acid is more stable than L-amino acid and is suitable for being used in the field. The D-amino acid and the D-amino acid derivatives have quite good anti-TMV activity (in the aspects of protection, treatment and passivation).

The invention discloses polyphenol compounds in aromatic tobaccos as well as a preparation method and application of the polyphenol compounds. The polyphenol compounds are obtained by separating from roots and stems of the aromatic tobaccos, and have a molecular formula of C18H18NaO6, and have a structure as shown in the specification. A preparation method of the polyphenol compounds comprises the following steps of extract extraction, silica-gel column chromatography and high-pressure liquid-phase chromatographic separation by taking roots and stems of the aromatic tobaccos as materials, and specifically comprises the following steps: crushing the roots and the stems of the aromatic tobaccos, ultrasonically extracting by alcohol, combining extracting liquor, filtering, reducing pressure and concentrating into extract; and carrying out silica-gel column chromatography on the extract by filling the column through a silica-gel dry method; carrying out gradient elution by adopting chloroform-acetone liquor with a volume ratio of (1:0)-(1:2); and separating and purifying 7:3 part of the eluant by using high-pressure liquid-phase chromatography to obtain the polyphenol compounds. A test proves that the polyphenol compounds disclosed by the invention have good cell activity for tobacco mosaic virus. Besides, the compounds disclosed by the invention are simple in structure, easy to artificially synthesize and good in activity, and can be used as guiding compounds for resisting the tobacco mosaic virus.

The invention discloses a chalcone compound in a higher plant desmodium renifolium and a preparation method and an application thereof. The chalcone compound is separated from desmodium renifolium, and the molecular formula is C21H24O6. The chalcone compound has the following structure as shown in the specification. The preparation method comprises the following steps: by taking a complete stool of desmodium renifolium as a raw material, carrying out extract extraction, column chromatography on silica gel and high-performance liquid chromatography separation, namely smashing the complete stool of desmodium renifolium, and fractionally carrying out ultrasonic treatment, filtering and reduced-pressure concentrating to form an extract; carrying out column chromatography on silica gel on the extract by a silica gel dry column-packing method; carrying out gradient eluting on a chloroform-acetone solution in a volume ratio of 1:0-1:2; and further carrying out separation and purification of high-performance liquid chromatography on an 8:2 part of the eluant to obtain the chalcone compound. Tests verify that the compound has good cell viability to the tobacco mosaic virus. The compound disclosed by the invention is simple in structure, easy to realize artificial synthesis and good in activity, and can be used as a guiding compound for resisting the tobacco mosaic virus.