52results about How to "Good activity against tobacco mosaic virus" patented technology

The present invention relates to a process for preparing 1,2,3-thiadiazole compounds, which can be used to prevent and treat hepatitis B and plant viruses.

The invention discloses an isocoumarin compound and a preparation method and an application thereof. The isocoumarin compound is separated from canella tailed spicebush root and leaf (Lindera caudata) and is named caudacoumarin A; the English name is caudacoumarin A; the molecular formula of the caudacoumarin A is C16H18O5; and the caudacoumarin A has the following structure as shown in the specification. The preparation method of the isocoumarin compound comprises the following steps: with canella tailed spicebush root and leaf as a raw material, extractum extraction, organic solvent extraction, MCI decoloration, column chromatography on silica gel, and high-pressure liquid chromatography separation. The application is an application of the isocoumarin compound in preparation of an anti-mosaic virus medicine. Through a test by a half-leaf method, the caudacoumarin A displays good anti-tobacco mosaic virus activity. The isocoumarin compound disclosed by the invention is simple in structure and good in activity, can be used as a lead compound of resisting the tobacco mosaic virus, and has a relatively good application prospect.

The invention discloses a butyrolactone lignan compound and a preparation method and an application thereof. The butyrolactone lignan is prepared from a P. ramigena, YNCA0361 strain by steps of fermentation, extraction, chromatography and purification. The molecular formula is C23H22O8 and the butyrolactone lignan compound has a structural formula shown in the specification, wherein the compound is named versicolatcone A. The preservation number of the P. ramigena, YNCA0361 strain is CGMCCNo.4824, and the preservation date is May 2, 2011. The preparation method comprises the following steps: by using fermented solids of the P. ramigena, YNCA0361 strain as raw material, carrying out organic solvent extraction; carrying out column chromatography on silica gel; carrying out MCI decoloring; carrying out reversed-phase column chromatography; carrying out high pressure liquid chromatography and the like. The butyrolactone lignan is applied to preparation of anti-tobacco mosaic virus drugs. The compound disclosed by the invention is novel in structure and good in activity, can be used as a lead compound for resisting the tobacco mosaic virus, and has a relatively good application prospect.

The invention discloses a hexacyclic alkaloid compound, a preparation method and an application thereof. The hexacyclic alkaloid compound is obtained through fermentation, extract extraction, chromatography and purification on a Penicilliumramigena (YNCA0361) strain, a molecular formula is C19H20N2O6, a structural formula is shown as the following, the compound is named as Penicilliumramigena A; a bacterial strain preservation number of the Penicilliumramigena (YNCA0361) strain is CGMCC No.4824, and the preservation date is 2nd, May, 2011. The preparation method comprises the steps of solid fermentation, extract extraction, slica column chromatography, and separation and purification by high pressure liquid chromatography. The application of the present invention is the application of the hexacyclic alkaloid compound for preparing tobacco mosaic virus medicines. The compound has good application prospect for preparing the tobacco mosaic virus medicines. The compound has good activity, and can be obtained through a biological fermentation approach, and can be used as the guiding compound for the tobacco mosaic virus medicines.

The invention discloses a chalcone compound in a higher plant desmodium renifolium and a preparation method and an application thereof. The chalcone compound is separated from desmodium renifolium, and the molecular formula is C21H24O6. The chalcone compound has the following structure as shown in the specification. The preparation method comprises the following steps: by taking a complete stool of desmodium renifolium as a raw material, carrying out extract extraction, column chromatography on silica gel and high-performance liquid chromatography separation, namely smashing the complete stool of desmodium renifolium, and fractionally carrying out ultrasonic treatment, filtering and reduced-pressure concentrating to form an extract; carrying out column chromatography on silica gel on the extract by a silica gel dry column-packing method; carrying out gradient eluting on a chloroform-acetone solution in a volume ratio of 1:0-1:2; and further carrying out separation and purification of high-performance liquid chromatography on an 8:2 part of the eluant to obtain the chalcone compound. Tests verify that the compound has good cell viability to the tobacco mosaic virus. The compound disclosed by the invention is simple in structure, easy to realize artificial synthesis and good in activity, and can be used as a guiding compound for resisting the tobacco mosaic virus.

The present invention discloses a parallel six-membered ring biphenyl compound (I), a preparation method and an application thereof. The preparation method is as follows: adopting tobacco whole plants as raw materials, extracting the raw materials with high-concentration methanol or high-concentration acetone/water or high-concentration ethanol/water, combining extracting solutions, carrying out filtering, concentrating the filtrate under reduced pressure to prepare an extract; loading the extract onto a column with a silica gel dry method for performing silica gel column chromatography; carrying out gradient elution with a chloroform-acetone solution, and further separating and purifying a 9:1 part of the eluent with high-pressure liquid phase chromatography to obtain the biphenyl compound. An activity test shows that the compound in the present invention has good inhibitory effects on tobacco mosaic viruses. The compound is simple in structure, has high activity, and can be taken as a pilot compound for resisting tobacco mosaic viruses.

The invention discloses a chromone derivative, a preparation method and application thereof. The chromone derivative is separated from a cassia leschenaultiana whole plant, the molecular formula is C18H20O4, and the compound is named as: 6-(2, 2-dimethyl-2H-chromene)-2-isopropyl-8-methoxy-4H-chromen-4-one. The preparation method of the chromone derivative includes: taking a cassia leschenaultianawhole plant as the raw material, and conducting crushing, extraction, silica gel column chromatography and high pressure liquid chromatography separation steps. Biological activity test indicates thatthe compound provided by the invention has a good inhibiting effect on tobacco mosaic virus. The compound provided by the invention is easily separable, has good activity, and can be used as a leading compound against tobacco mosaic virus.

The invention discloses a chromone derivative extracted from Cassia leschenaultiana as well as a preparation method and application of the chromone derivative. The chromone derivative is separated from complete Cassia leschenaultiana, the molecular formula of the chromone derivative is C18H20O3, and a compound of the chromone derivative is 6-(2,2-dimethyl-2-H-chromene)-2-isopropyl-8-methyl-4H-chromen-4-one. The preparation method of the chromone derivative comprises the following steps: taking the complete Cassia leschenaultiana as a raw material, smashing the complete Cassia leschenaultiana,extracting the smashed complete Cassia leschenaultiana and performing slica column chromatography and HPLC separation. Through a test for biological activity of the compound, the compound has a good effect of inhibiting a tobacco mosaic virus. The compound is easy to separate, is good in activity and can serve as a guiding compound for resisting the tobacco mosaic virus.

The invention discloses a flavonoid compound, and a preparation method and an application of the flavonoid compound. The flavonoid compound is separated from cassia leschenaultiana. A molecular formula of the flavonoid compound is C20H20O5; and the compound is named as 6,4'-dimethoxy-7-(3-hydroxypropyl)-flavone and has the following structure as shown in the specification. The preparation method comprises the steps of extract extraction, silicagel column chromatography, high pressure liquid chromatography separation and gel column chromatography. The application is an application of the flavonoid compound in preparing a tobacco mosaic virus resistance medicine. The compound has good application prospects in preparing the tobacco mosaic virus resistance medicine. The compound is simple in structure and good in activity, and can serve as a pilot compound of the tobacco mosaic virus resistance medicine.

The invention discloses chalcone compounds and a preparation method and application thereof. The chalcone compounds are separated from Desmodium renifolium and have a molecular formula of C20H20O5 and the structure of the chalcone compounds is shown in the specification. The preparation method of the chalcone compounds comprises the following steps: extracting extract from the whole Desmodium renifolium strain serving as a raw material and separating by column chromatography on silica gel and high pressure liquid chromatography. Particularly, the preparation method of the chalcone compounds comprises the following steps: pulverizing the whole Desmodium renifolium strain, carrying out ultrasonic extraction with methanol, mixing extractive solution, filtering, concentrating under reduced pressure to obtain an extract; loading the extract on the column by a silica gel dry method to carry out column chromatography on silica gel, gradiently eluting with chloroform-acetone solution having a volume ratio of (1:0) to (1:2), further separating and purifying 8:2 of eluate by high pressure liquid chromatography to obtain the chalcone compounds. The experimental results show that the chalcone compounds have good cell activity to tobacco mosaic virus (TMV). The chalcone compounds disclosed by the invention are simple in structure, can be synthesized artificially, have good activity and can be used as anti-TMV lead compounds.

The invention discloses polyphenol compounds in aromatic tobaccos as well as a preparation method and application of the polyphenol compounds. The polyphenol compounds are obtained by separating from roots and stems of the aromatic tobaccos, and have a molecular formula of C18H18NaO6, and have a structure as shown in the specification. A preparation method of the polyphenol compounds comprises the following steps of extract extraction, silica-gel column chromatography and high-pressure liquid-phase chromatographic separation by taking roots and stems of the aromatic tobaccos as materials, and specifically comprises the following steps: crushing the roots and the stems of the aromatic tobaccos, ultrasonically extracting by alcohol, combining extracting liquor, filtering, reducing pressure and concentrating into extract; and carrying out silica-gel column chromatography on the extract by filling the column through a silica-gel dry method; carrying out gradient elution by adopting chloroform-acetone liquor with a volume ratio of (1:0)-(1:2); and separating and purifying 7:3 part of the eluant by using high-pressure liquid-phase chromatography to obtain the polyphenol compounds. A test proves that the polyphenol compounds disclosed by the invention have good cell activity for tobacco mosaic virus. Besides, the compounds disclosed by the invention are simple in structure, easy to artificially synthesize and good in activity, and can be used as guiding compounds for resisting the tobacco mosaic virus.

The invention relates to two optical pure isomers called NK007 for short, synthesis thereof and application thereof in the aspects of plant virus resistance and immunoregulatory activity. Compounds NK007-1 and NK007-2 have good anti-tobacco-mosaic-virus activity, and can be used for treating plant virus diseases. The compounds NK007-1 and NK007-2 have good immunoregulatory activity and can be used for preparing medicine treating rheumatic arthritis. The method for testing the immunoregulatory activity of the compounds can be used for screening novel arthritis medicine. Please see the chemical structural formulae of the compounds in the specifications.

The invention discloses a flavonoid compound and a preparation method and application thereof. The flavonoid compound is separated out and obtained from cassia leschenaultiana, a molecular formula of the flavonoid compound is C20H18O6, the compound is named as 6,4'-dimethoxy-7-(3-hydroxypropan1-one)-flavone, and an English name is 6,4'-dimethoxy-7-(3-hydroxypropan1-one)-flavone. The flavonoid compound is of a following structure shown in the description. The preparation method comprises the steps of extractum extraction, silica column chromatography, high pressure liquid chromatography separation and gel column chromatography. The application is that the flvonoid compound is applied to preparing tobacco mosaic virus resisting medicine. The compound disclosed by the invention has a good application prospect in preparing the tobacco mosaic virus resisting medicine. The compound disclosed by the invention has the advantages of simple structure and good activity and can serve as a pilot compound of the tobacco mosaic virus resisting medicine.

The invention discloses a chromone derivative from Cassia pumila and a preparation method and application thereof. The chromone derivative is isolated from a whole plant of Cassia pumila and has a molecular formula of C16H16O6 and a compound name of 8-hydroxy-2-(3-hydroxypropyl)-5-(isobutyryl)-4H-4-chromone. The preparation method of the chromone derivative includes: crushing the whole plant of Cassia pumila, extracting, carrying out silica gel column chromatography, and carrying out high-pressure liquid chromatographic separation. Biological activity test on the chromone derivative herein shows that the chromone derivative can well inhibit tobacco mosaic virus. The chromone derivative is easy to isolate, has good activity and may act as a pilot compound to resist tobacco mosaic virus.