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Chalcone compounds and preparation method and application thereof.

A kind of compound, technology of chalcone, applied in the field of chalcone compound and preparation thereof

Inactive Publication Date: 2014-07-16
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the present invention, a new chalcone compound with anti-tobacco mosaic virus activity is obtained from the leech locust, which has not been reported so far

Method used

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  • Chalcone compounds and preparation method and application thereof.
  • Chalcone compounds and preparation method and application thereof.
  • Chalcone compounds and preparation method and application thereof.

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preparation example Construction

[0026] Chalcone compound C of the present invention 20 h 20 o 5 The preparation method comprises extract extraction, silica gel column chromatography, high-pressure liquid chromatography separation steps, specifically comprises:

[0027] A. Extraction of extract: take a sample of the leech locust, crush it to 20-40 mesh, use 90-99% methanol to ultrasonically extract 3-5 times, each time for 30-60 min, combine the extracts, filter, and concentrate under reduced pressure to obtain extract;

[0028] B. Silica gel column chromatography: the extract is packed with 160~200 mesh silica gel dry method with a weight ratio of 2~3 times for silica gel column chromatography; chloroform-acetone solution with a volume ratio of 1:0~1:2 Perform gradient elution, pool the same fractions, collect and concentrate the eluate from each fraction;

[0029] C. High-pressure liquid chromatography separation: the 8:2 part of the eluent in step B is further separated and purified by high-pressure li...

Embodiment 1

[0038] The samples of leech leech came from Xishuangbanna, Yunnan. Sampling 2.2 kg of the leech locust was crushed to 30 mesh, extracted 3 times with 95% methanol by ultrasonic, 30 min each time, the extracts were combined, filtered, concentrated under reduced pressure to obtain an extract, 88.5 g of extract was obtained. The extract was dissolved in pure methanol with a weight ratio of 2.5 times, and then mixed with 120 g of 100-mesh crude silica gel, and 1.5 kg of 160-mesh silica gel was packed into a column for silica gel column chromatography, and the volume ratio was 1:0, 20:1 , 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 8 The chloroform-acetone elution part of :2 was separated by Agilent 1100 semi-preparative high-performance liquid chromatography, with 58% methanol as mobile phase, Zorbax SB-C18 (21.2 × 250 mm, 5 μm) preparative column as stationary phase, Th...

Embodiment 2

[0040] The leech leech sample comes from Dehong, Yunnan. 3.5 kg of the leech leech was crushed to 40 meshes, extracted 5 times with 90% methanol by ultrasound, 45 min each time, the extracts were combined, filtered, and concentrated under reduced pressure into an extract, and 158 g of extract was obtained. The extract was dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 300 g of 80-mesh crude silica gel, and 1.5 kg of 200-mesh silica gel was packed into a column for silica gel column chromatography, and the volume ratio was 1:0, 20:1 , 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same parts to obtain 8 parts, wherein the volume ratio is 8 The chloroform-acetone elution part of :2 was separated by Agilent 1100 semi-preparative high-performance liquid chromatography, with 58% methanol as mobile phase, Zorbax SB-C18 (21.2 × 250 mm, 5 μm) preparative column as stationary phase, The flow rate was 2...

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Abstract

The invention discloses chalcone compounds and a preparation method and application thereof. The chalcone compounds are separated from Desmodium renifolium and have a molecular formula of C20H20O5 and the structure of the chalcone compounds is shown in the specification. The preparation method of the chalcone compounds comprises the following steps: extracting extract from the whole Desmodium renifolium strain serving as a raw material and separating by column chromatography on silica gel and high pressure liquid chromatography. Particularly, the preparation method of the chalcone compounds comprises the following steps: pulverizing the whole Desmodium renifolium strain, carrying out ultrasonic extraction with methanol, mixing extractive solution, filtering, concentrating under reduced pressure to obtain an extract; loading the extract on the column by a silica gel dry method to carry out column chromatography on silica gel, gradiently eluting with chloroform-acetone solution having a volume ratio of (1:0) to (1:2), further separating and purifying 8:2 of eluate by high pressure liquid chromatography to obtain the chalcone compounds. The experimental results show that the chalcone compounds have good cell activity to tobacco mosaic virus (TMV). The chalcone compounds disclosed by the invention are simple in structure, can be synthesized artificially, have good activity and can be used as anti-TMV lead compounds.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a chalcone compound extracted from the leech locust and its preparation method and application. Background technique [0002] Kidney leaf leech is a small shrub of the leguminous family, mainly distributed in Yunnan, Shaanxi, Sichuan, Hubei, Hunan, Jiangxi, Fujian, Zhejiang, Jiangsu; it is also distributed in Korea and Japan. The root and the whole plant of the aboveground part of the root and the whole plant are used for medicinal purposes, and have the functions of dispelling wind, activating blood circulation, diuresis, and killing insects. Studies at home and abroad have shown that the main active ingredients of M. chinensis are flavonoids, including flavonoids, isoflavones, and chalcones. Chalcone refers to a compound containing 1,3-diphenylpropenone structure in its molecule. They are widely distributed in the roots, leaves and bark of various medicinal...

Claims

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Application Information

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IPC IPC(8): C07C49/83C07C45/78A01P1/00
CPCA01N35/04C07C45/78C07C49/83C07C2602/08
Inventor 胡秋芬李银科叶艳清杜刚杨海英李萍艳高雪梅
Owner YUNNAN MINZU UNIV
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