581 results about "Tobacco mosaic virus" patented technology

The present invention provides one kind of 1, 2, 3-thiobiazole-5-formamide compounds and their synthesis process and pesticide bioactivity screening. The screening system includes the determination of tobacco mosaic disease resisting activity, tobacco mosaic disease virus resistance inducing activity, and pesticidal and fungus-inhibiting activity. The related compounds are 1, 2, 3-thiobiazole-5-formamide derivatives with the general expression as shown, and include 7 hetero cycle containing 1, 2, 3-thiobiazole-5-formamide derivatives, 2 benzene ring containing 1, 2, 3-thiobiazole-5-formamide derivatives, and 2 aliphatic radical containing 1, 2, 3-thiobiazole-5-formamide derivatives.

The invention relates to a ferulic acid shown in a general formula (I) and the application of derivatives thereof to pesticides. When used as novel anti-phytoviral agents, the derivatives can well inhibit tobacco mosaic viruses, pepper viruses, tomato viruses, sweet potato viruses, potato viruses, melon viruses, maize dwarf mosaic viruses and the like, and effectively prevent and control the virus diseases of various crops such as tobacco, peppers, tomatoes, melons, grains, vegetables, beans and the like, and are particularly suitable for preventing and controlling tobacco mosaic. Meanings of each group are shown in specifications.

The invention discloses a vanillin derivative containing dithioacetals, a preparation method and a purpose thereof. A structural general formula of the derivative is shown in a specification, wherein, R1 is a substituted aromatic ring, heterocycle or 1,1-dichloroethylene; a methyl atom, a nitro atom or a halogen atom is contained on an o-position, a meta-position and a para-position of the aromatic ring, the halogen atom can be fluorine, chlorine, or bromine; the heterocycle is five-membered or six-membered heterocycle, and one halogen atom is contained on the heterocycle. R2 is substituted aromatic ring, 1-propanol or heterocycle; fluorine or chlorine is contained on para-position of the aromatic ring, and the heterocycle is furan ring. The compound can inhibit plant virus such as tobacco mosaic virus, cucumber mosaic virus and potato y potyvirus.

The invention provides bacillus velezensis for inhibiting virus and promoting plant growth and application of a bacillus velezensis SNB55 strain in prevention and treatment of plant virus diseases andplant fungal diseases and promotion of plant growth. The bacillus velezensis for inhibiting virus and promoting plant growth and the application of the bacillus velezensis have the beneficial effectsthat the bacillus velezensis has no toxicity and pathogenicity, is safe to human and livestock, is environment-friendly, and has high stress resistance and stable antibacterial activity; the bacillusvelezensis can inhibit phytopathogens such as bacillus cereus, tobacco mosaic virus, tomato yellow leaf curl virus, fusarium wilt of cucumbers, tobacco target spot pathogens, phytophthora capsici, rice sheath blight disease and botrytis cinerea, can be secrete indoleacetic acid to promote seed germination and plant growth; and the strain is a multifunctional biocontrol bacterium and has a wide application prospect.

The invention provides a pesticide composition, and in particular relates to an active pesticide composition, compounding one or more of toxic fluoride phosphate, the weight ratio of toxic fluoride phosphate to other active substances is 30:0.15 to 3.3:30, and the content of effective components by weight is 1%-90%. The compound composition can be processed into water dispersible powder, suspending agent, microemulsion, aqueous emulsion, water dispersible granule, dry suspension emulsion and seed coating agent and the like, after accessory ingredient or carrying agent is added. The composition can be used to prevent and treat virus diseases caused by rice stripe virus, rice dwarf virus, maze rough dwarf virus, rice black-streaked dwarf virus, tomato virus, cucumber mosaic virus and tobacco mosaic virus, and has the beneficial effects of enhanced effect, concurrent treatment, postponed pesticide resistance, decreased dosage of toxic fluoride phosphate and reduced cost.

The invention provides a formulation composite comprising toxic fluoride phosphate and at least one growth regulator. According to the weight part, the toxic fluoride phosphate accounts for 10%-80% of the formulation composite, the growth regulator accounts for 0.0001%-2% of the formulation composite, the formulation composite also comprises an assistant, an surface active agent, a solvent and a carrier accepted by agriculture, and the formulation composite can be prepared into wettable powder, suspending agent, missible oil, microemulsion, emulsion in water, water dispersible granule, dry suspending agent, seed coating agent and other agents. The formulation composite can be used for preventing and curing tobacco mosaic virus, cucumber mosaic virus, and various virus diseases caused by viruses on rice, such as tobacco mosaic, cucumber mosaic, tomato virus disease, pepper virus disease, rice virus disease and the like and achieves the effects of improving efficacy, treating in a compatible manner, delaying medicine resistance, reducing the using amount of toxic fluoride phosphate and the like.

The invention relates to a method for detecting tobacco viruses by a reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) technology, which comprises the steps of tobacco virus total ribonucleic acid (RNA) extraction, RT-LAMP reaction, electrophoresis detection and virus type determination, wherein tobacco viruses are selected from two or more than two kinds of viruses from cucumber mosaic viruses (CMV), potato viruses Y (PVY), tobacco etch viruses (TEV), tobacco mosaic viruses (TMV) and tobacco vein banding mosaic viruses (TVBMV). LAMP primers are designed according to a case protein gene conserved region of the tobacco viruses, and a fast, sensitive and high-specificity method is built for detecting various main tobacco viruses by the one-step RT-LAMP technology. A newmeasure is provided for the fast detection of the tobacco viruses.

The invention discloses a Harpin coding gene (i) hrpZPsgS1( / i) and an application of a harpinZPsgS1 protein expressed by the Harpin coding gene (i) hrpZPsgS1( / i). If the obtained harpinZPsgS1 protein is used for treating tobacco plants, the growing of the tobacco plants is promoted; and the resistance of the tobacco plants against tobacco mosaic viruses and tobacco phytophthora diseases is improved obviously, wherein the resistance is not only limited in parts treated by the harpinZPsgS1 protein but also acts on the peripheral leaves and the whole plants, and the expression of PR (pathogenesis related) protein is carried out in the treating process, so that the hrpZPsgS1( / i) can induct the plant systems to obtain disease resistance. The result shows that the hrpZPsgS1 has the application value of being developed into a biogenic pesticide.

The invention discloses a preparing method for a tobacco mosaic virus nanowire composite material with the surface functionalized. The preparing method includes the following steps that while tobacco mosaic virus head-tail assembly is carried out on the acidic condition, dopamine and an oxidant are subjected to in-situ oxidative polymerization on the outer surface of virus protein to form tobacco mosaic virus nanowires coated with polydopamine, and on the alkaline condition, the own reducibility and reactivity of the surface polydopamine are used for further loading gold nanoparticles and modifying bioactive molecules containing amino groups or sulfydryl groups in a functionalized mode without extra reductant or chemical modification so as to obtain the tobacco mosaic virus nanowire composite material with the surface functionalized. The preparing method has the advantages of being simple in process, convenient, efficient and capable of being widely applied to biological catalysis, biological imaging, tumor targeted probes, nano-drug carriers and other fields of biomedical new nano materials.

The invention discloses a coumarin compound and a preparation method and application thereof. The coumarin compound is obtained by being separated from tobacco rhizome, the molecular formula of the coumarin compound is C17H14O5, and the structure of the coumarin compound is as shown in the specification. The preparation method of the coumarin compound comprises the following steps: smashing a tobacco rhizome sample, then ultrasonically extracting for 3-5 times by using 90%-99% ethanol; merging extracting solutions; filtering, carrying out reduced-pressure concentration to obtain an extractum; and initially separating the extractum by adopting silica-gel column chromatography, and then further separating by adopting high performance liquid semi-preparation chromatography to obtain the coumarin compound. The coumarin compound disclosed by the invention has the advantages of simple structure, easiness for artificial synthesis realization and better cell activity on tobacco mosaic viruses and can be used as a lead compound resisting the tobacco mosaic viruses.

The invention relates to a flavanone compound and application thereof, belonging to the technical field of tobacco chemistry. The flavanone compound is obtained by the following steps: smashing a tobacco rootstalk sample to 30 meshes, then carrying out ultrasound extraction three times with 95% ethanol, and mixing extracting solutions; filtering, and concentrating the mixed extracting solution atreduced pressure to form extract; dissolving the extract with analytically pure methanol and then carrying out primary separation with silica gel column chromatography; further separating with high performance liquid semi-preparative chromatography so as to obtain the compound. The flavanone compound is named as 5-hydroxyl-6-methyl-3',7-dimethoxyl-8-aldehydel-flavanone. The flavanone compound hasthe advantages of low preparation cost and good tobacco mosaic virus resisting activity, and can be used for preparing tobacco mosaic virus resisting active preparations; and according to the invention, a beneficial new way is provided for comprehensive utilization of tobacco resources.

The invention discloses a myricetin derivative containing amide thioether thiadiazole, of which a general formula is represented as follows, wherein R is hydrocarbyl groups, phenyl groups, substituted phenyl groups, heteroaromatic ring groups or substituted heteroaromatic ring groups. The compound has high treatment and prevention effect against tobacco mosaic virus (TMV), has high anti-plant virus activity and can be used for preparing anti-plant virus pesticides. The compound has high inhibition activity on Xanthomonas axonopodis and Xanthomonas oryzae and can be used for preparing agricultural bactericides.

The invention discloses a special organic and inorganic compound pesticide fertilizer for tobacco. The special organic and inorganic compound pesticide fertilizer comprises the following components in parts by weight based on dry materials: 30-40 parts of organic active fertilizer, 12-20 parts of ammonium nitrate, 20-30 parts of calcium superphosphate, 16-32 parts of potassium nitrate, 5-10 parts of magnesium sulfate, 1-5 parts of zinc sulfate, 1-4 parts of sodium silicate, 0.1-1 part of borax, 0.02-0.1 part of ammonium molybdate, 0.5-1 part of insecticide, 0.5-1 part of bactericide and 0.5-1 part of coating agent. Compared with a conventional fertilizer, the special organic and inorganic compound pesticide fertilizer for the tobacco, produced according to the formula of the invention, has the advantages that 20-30 percent employees can be saved, the application of a pesticide is reduced, underground insects such as agrotis ypsilon and grubs, and root black rot can be controlled, a certain resistance is achieved for tobacco mosaic virus, the ratio of first-class tobacco is increased by 3.2-5.7 percent, and the output value is increased by 294.5-2512yuan / hm<2>.

The present invention relates to a novel microorganism Bacillus amyloliquefaciens KTGB0202 and a method for controlling plant pathogens using the same. More particularly, the present invention relates to a novel microorganism Bacillus amyloliquefaciens KTGB0202 (accession number: KCTC 10564BP) bacterium which has an excellent control effect against crop powdery mildew and a broad spectrum of antifungal activity against plant pathogenic fungi and inhibits tobacco mosaic virus infection, as well as an eco-friendly bacterial culture broth for controlling powdery mildew, which contains the same. Also, the present invention relates to antifungal substance KTGB0202 AFO1 obtained by extraction and purification from the Bacillus amyloliquefaciens KTGB0202 bacteria. The inventive Bacillus amyloliquefaciens KTGB0202 bacteria have a broad spectrum of antifungal activity, and are excellent in activity continuance and used for controlling various plant pathogens, including powdery mildew.

The invention relates to the field of genetic engineering, in particular to preparation of an infectious clone vector of a Pepper mild mottle virus (PMMoV) of RNA (ribonucleic acid) plant viruses and tobacco mosaic viruses, pathogenesis analysis on an infectiously cloned agrobacterium tumefaciens strain with the pepper mild mottle virus and exogenous proteins infectiously cloned and expressed by the aid of the PMMoV. The preparation, the pathogenesis analysis and exogenous proteins have the advantages that genomes of Shanghai bay isolate of the pepper mild mottle virus are constructed onto efficient plant expression vectors pCB301-CH by the aid of a genetic recombination process, the recombinant vectors are transferred into the agrobacterium tumefaciens strain by means of electroporation, and accordingly the virus vector with infection ability can be obtained; the plant expression vectors can be constructed by means of infectious cloning, and the exogenous proteins can be efficiently and stably expressed in host plants; mature systems can be provided for studying structures and functions of the virus genomes and studying interaction between the virus and hosts.

The invention discloses a novel isocoumarin compound. The isocoumarin compound has the chemical name of 2-(hydroxymethyl)-8,8-dimethyl-9,10-dihydropyrano[4,3-f]benzopyran-4 (8H)-one, the molecular formula of C15H16O4 and the structure represented by a formula shown in the specification. The invention further discloses a preparation method for preparing the isocoumarin compound from tobacco. The invention further discloses use of the compound, and shown by activity tests, the compound plays a good role in inhibiting tobacco mosaic virus. The compound disclosed by the invention is simple in structure and relatively good in tobacco mosaic virus resisting activity and can be applied to the research and development of tobacco mosaic virus resisting pesticide preparations as a tobacco mosaic virus resisting lead compound.

The invention provides a fertilizer for cultivation of tobacco. The fertilizer consists of the components by weight as follows: 0.5-1.5 parts of a composite microbial agent, 3-7 parts of an activating agent, 3-7 parts of binder, and 86-92 parts of nitrogen, phosphorus and potassium fertilizer. The effective components of the composite microbial agent are Bacillus licheniformis, Bacillus pumilus, Brevibacillus laterosporus, Bacillus megatherium and Silicate bacteria. The invention further provides a preparation method and an application of the fertilizer. The product provided by the invention can remarkably improve crumb-structure of soil for cultivation of tobacco, and microflora distribution and micro-environment of the soil. A plenty of derivatives are generated, thereby chelating or blocking damage of heavy metals, and absorption and assimilation of tobacco to nutrient elements are improved, the drought resisting and disease resisting abilities of tobacco are enhanced, damage of diseases and insects caused by continuous cropping of tobacco in successive years are effectively prevented, and in particular, tobacco mosaic virus and brown spot disease are specially prevented and treated. According to the invention, the raw materials are low in cost and easy to obtain and the preparation and using methods are simple and easy to operate.

The invention belongs to the field of biotechnology, and provides an RNAi vector pGEM-PCTRi capable of mediating the resistance to tobacco viral diseases in China. Analyzing the genome characteristics of main virus groups in China, which comprise Potato Virus Y (PVY), Cucumber Mosaic Virus (CMV) and Tobacco Mosaic Virus (TMV) and then selecting three segments of conserved effective sequences capable of inducing RNAi in PVY CP, CMV RNA1 and TMV CP; and integrating the three segments in a tabling manner and constructing an inverted repeat sequence so as to obtain an RNAi vector pGEM-PCTRi. Massproduction of dsRNA can be realized in escherichia coli by utilizing the vector, and the resistance of tobacco to PVY, CMV and TMV can be induced to generate after the tobacco treatment. The invention has the potential of being applicable to the prevention of viral diseases for tobacco farmland production, and endowed with the advantages of broad spectrum, high efficiency, no reference to transgenosis, and the like.

The invention discloses an indole carbazole compound which is characterized by having a structure as shown in a formula I, a formula II, a formula III or a formula IV described in the specification. The indole carbazole has anti-TMV (tobacco mosaic virus) activity.

The invention discloses a 1,4-pentadiene-3-ketone derivative containing benzotriazinone. The 1,4-pentadiene-3-ketone derivative containing benzotriazinone is characterized by having the general formula as shown in the specification, wherein R1 is phenyl, substituted phenyl (p-fluorophenyl, p-chlorophenyl, o-chlorophenyl, 2-methyoxyphenyl, 4-methyoxyphenyl, 4-methylphenyl, 2,4-dimethyoxyphenyl, 3,4-dimethyoxyphenyl, cinnamaldehyde group, 3-nitrophenyl, 2-chloro-5-nitrophenyl and the like), heterocyclic group (furyl, thienyl, 2-pyridyl, 3-pyridyl, 4-pyridyl, pyrryl and the like) or substituted aromatic heterocyclic group (5-methyl thiazole, 5-methyl-2-thienyl, 4-bromo-2-thienyl and the like), and R2 is a hydrogen atom, methyl (ethyl), methoxyl (ethyoxyl) and the like. The compound disclosedby the invention has relatively high inhibiting activity for citrus canker bacteria, ralstonia solanacearum and tobacco mosaic viruses so as to be used for preparing an agricultural bactericide and anantiviral agent.

The invention discloses quinoxaline-containing 1,4-pentadiene-3-one derivatives. The quinoxaline-containing 1,4-pentadiene-3-one derivatives are characterized by having a general formula which is as shown in the specification, wherein R1 is a phenyl, a substituted phenyl or a substituted aromatic heterocycle, and R2 one or more hydrogen atoms, methoxyls, nitryls, methyls, trifluoromethyls or halogen atoms contained on the 5-position, 6-position, 7-position and the 8-position of a quinoxaline structure. The compound provided by the invention has very good treating, protecting and passivating effects on TMV (Tobacco Mosaic Virus), shows high resistance to plant virus, and can be used for preparing a plant virus resisting pesticide.

The invention provides a tobacco mosaic virus (TMV) resistant bacillus amyloliquefaciens strain Z5. The strain was collected in the China Center for Type Culture Collection (CCTCC) on March 24, 2014, with the collection number of CCTCC NO:M2014102. Fermentation liquor of the strain Z5 has inhibiting effects on the TMV. The in-vitro TMV inhibition ratio of the aseptic fermentation liquor is measured to be above 96% by utilizing a local lesion method. Coarse protein liquor prepared by precipitating the fermentation liquor has inhibiting effects on the TMV as well, has good antivirus effects and obvious control effects in the fields and can be used for effectively reducing the occurrence of TMV.

The invention relates to isolation cloning and breeding application of a tobacco mosaic virus (TMV) resistant N'au gene. A nucleotide sequence of the TMV resistant N'au gene is shown as SEQ ID NO.1, and amino acid of polypeptide coded by the TMV resistant N'au gene is shown as SEQ ID NO.2. A non-transgenic TMV-resistant tobacco variety can be obtained by trans-breeding an N'au gene contained in germplasm resources into a TMV-sensitive main planting tobacco variety through a conventional breeding means. The N'au gene in the main planting tobacco variety has a homologous sequence high in nucleotide rate, so that short introgression fragment single plants carrying the N'au gene can be obtained easily through conventional breeding so as to obtain a TMV-resistant variety low in linkage drag. The gene can resist strains U1 and Cg of TMV at the same time. The novel antiviral gene N'au has great application prospect in cultivation of TMV-resistant tobacco.

The invention relates to the field of microorganisms, in particular to a biocontrol bacterium for efficiently producing lipopeptide substances. The biocontrol bacterium is Bacillus velezensis HN-2 andpreserved in the China Center for Type Culture Collection on June 20th, 2018, and the preservation number is CCTCC NO:M 2018382. The invention also discloses a fermentation technology of the Bacillusvelezensis HN-2. It is found through activity testing that an extract of fermentation broth of the strain has a protective function on tobacco when the tobacco mosaic virus (TMV) infects the tobacco,the tobacco defense reaction can be induced, the resistance of the tobacco to the TMV is improved, and the economic benefits are relatively high.

The invention relates to chitosan oligosaccharide Schiff base phosphonate, which has the structural formula shown in the specification, and in the formula, n is 2-20. The preparation method comprises the following steps of: carrying out chemical modification on chitosan oligosaccharide with salicylaldehyde and substituted salicylaldehyde by adopting a microwave radiation method to obtain a chitosan oligosaccharide Schiff base (S-COS), and then carrying out addition reaction on the S-COS and diethyl phosphite to obtain the chitosan oligosaccharide Schiff base phosphonate. The chitosan oligosaccharide Schiff base phosphonate has better activity for resisting tobacco mosaic virus.

The invention discloses an isocoumarin compound and a preparation method and an application thereof. The isocoumarin compound is separated from canella tailed spicebush root and leaf (Lindera caudata) and is named caudacoumarin A; the English name is caudacoumarin A; the molecular formula of the caudacoumarin A is C16H18O5; and the caudacoumarin A has the following structure as shown in the specification. The preparation method of the isocoumarin compound comprises the following steps: with canella tailed spicebush root and leaf as a raw material, extractum extraction, organic solvent extraction, MCI decoloration, column chromatography on silica gel, and high-pressure liquid chromatography separation. The application is an application of the isocoumarin compound in preparation of an anti-mosaic virus medicine. Through a test by a half-leaf method, the caudacoumarin A displays good anti-tobacco mosaic virus activity. The isocoumarin compound disclosed by the invention is simple in structure and good in activity, can be used as a lead compound of resisting the tobacco mosaic virus, and has a relatively good application prospect.

The invention discloses an indol-pyrrolidine-2-ketone alkaloid and a preparation method and application thereof. The indol-pyrrolidine-2-ketone alkaloid is obtained after fermentation, extraction, chromatography and purification by using a Penicilliumramigena, YNCA0361 bacterial strain, the molecular formula of the indol-pyrrolidine-2-ketone alkaloid is C21H24N2O7, the structural formula of the indol-pyrrolidine-2-ketone alkaloid is FORMULA, and the compound is called after aspergillineD; the preservation number of the Penicilliumramigena, YNCA0361 bacterial strain is CGMCC (China General Microbiological Culture Collection Center) No. 4824, and the preservation date is May 2nd, 2011. By taking the solid yeast of the Penicilliumramigena, YNCA0361 bacterial strain as a raw material, the preparation method comprises the steps of organic solvent extraction, slica column chromatography and high pressure liquid chromatography separation. The application is that the alkaloids compound is applied to the preparation of anticancer and anti-tobacco mosaic virus drugs. The compound is novel in structure and good in activity, can serve as an anticancer drug or a lead compound for resisting tobacco mosaic virus, and has a better application prospect.

The invention discloses a sesquiterpene compound which is prepared through supercritical fluid chromatography and is novel in structure. The compound is named as nicotianasesterpene-F, wherein the molecular formula of nicotianasesterpene-F is C16H20O2, and the nicotianasesterpene-F has the following structure (please see the structure in the specification). The invention further discloses application of the compound. Activity tests show that nicotianasesterpene-F has a very good restraining effect on tobacco mosaic viruses and can be used for research and development on tobacco mosaic virus prevention pharmaceutic preparations as the lead compound for preventing tobacco mosaic viruses.

The invention relates to 13a substituted phenanthroindolizidine alkaloid derivatives and 14a substituted phenanthroquinolizidine alkaloid derivatives, preparation methods and anti-plant virus activities thereof, wherein various groups in a formula are defined in an instruction. The 13a substituted phenanthroindolizidine alkaloid derivatives and the 14a substituted phenanthroquinolizidine alkaloid derivatives have good light stability and good thermal stability, show excellent anti-plant virus activities, and can be provided for well inhibiting tobacco mosaic virus (TMV).

The invention discloses an application of an A-ring trihydroxyl substituted pentacyclic triterpene compound to preparation of an antibacterial or anti-tobacco mosaic virus drug. The general formula of the compound is as shown in the specification, wherein hydroxyls of C1, C2 and C3 are respectively in alpha configuration or beta configuration; R1 and R2 are selected from hydrogen or methyl and are different from each other; R3 is selected from -COOH, -CH2OH, -CHO, -COOR1, -CONH2, -CONHR1 and -CONR1R2; R1 and R2 are selected from alkyl containing 1-15 carbon atoms, substituted or unsubstituted phenyl and substituted or unsubstituted phenyl alkyl; and the substituent group is selected from halogen, hydroxyl, cyan, amino, nitryl, sulfydryl or phenyl, acyl, aryl, alkoxy and alkyl containing 1-15 carbon atoms. The compounds have antibacterial or anti-tobacco mosaic virus activity, have extremely high bacteriostatic activity especially to gram positive bacteria, phytophthora nicotiana and tobacco mosaic virus, and have a good application prospect in fields of medicines and pesticides.