Preparing method for tobacco mosaic virus nanowire composite material with surface functionalized

Abstract

The invention discloses a preparing method for a tobacco mosaic virus nanowire composite material with the surface functionalized. The preparing method includes the following steps that while tobacco mosaic virus head-tail assembly is carried out on the acidic condition, dopamine and an oxidant are subjected to in-situ oxidative polymerization on the outer surface of virus protein to form tobacco mosaic virus nanowires coated with polydopamine, and on the alkaline condition, the own reducibility and reactivity of the surface polydopamine are used for further loading gold nanoparticles and modifying bioactive molecules containing amino groups or sulfydryl groups in a functionalized mode without extra reductant or chemical modification so as to obtain the tobacco mosaic virus nanowire composite material with the surface functionalized. The preparing method has the advantages of being simple in process, convenient, efficient and capable of being widely applied to biological catalysis, biological imaging, tumor targeted probes, nano-drug carriers and other fields of biomedical new nano materials.

Description

technical field [0001] The invention relates to the field of new nanometer materials. More specifically, it relates to a method for preparing a surface-functionalized tobacco mosaic virus nanowire composite material. Background technique [0002] Nanocomposite materials are a new type of nanomaterials, which not only have the advantages of small size effect, high specific surface area, various morphology characteristics and assembly forms of nanomaterials, but also can be combined with other inorganic, organic, polymer and biological functions. The special properties of materials have been widely developed in the fields of industrial high-efficiency catalysis, biomedical engineering and other new functional materials. Therefore, the development of new one-dimensional nanowire composites has great potential application value in biocatalysis, bioimaging, tumor targeting probes and nano drug carriers. [0003] Polydopamine is formed by oxidative polymerization of dopamine in ...

AI Technical Summary

Problems solved by technology

However, the coating materials used in the existing methods are all conductive polymers, which are not biocompatible and cannot be applied in the biomedical field.

[0005] In addition, although the method of using tobacco mosaic virus as a template and carrier to load gold nanoparticles and functional modification molecules on its surface has been realized (for example, Chinese patent CN103706800 expresses hexahistidine-modified tobacco by molecular biology methods) The loading of gold nanoparticles on the surface of tobacco mosaic virus by adding a reducing agent to the mosaic virus coat protein; Chinese patent CN102578695 is through electrostatic mutual attraction, and under the action of a reducing agent, it is deposited on the surface of tobacco mosaic virus by chemical deposition Loaded with gold nanoparticles; Chinese patent CN103613647 expresses a cysteine-mutated tobacco mosaic virus coat protein through molecular biology methods, and then modifies fluorescent molecules and anticancer drugs to the surface of the cysteine-mutated protein to prepare Nano drug carrier), but these methods have complex operation steps, long preparation period, need molecular biological methods to modify gene sequence and express protein, need to add additional reducing agent to reduce to obtain gold nanoparticles and other problems and defects

[0006] So far, the use of biocompatible polydopamine for coating and the preparation of one-dimensional nanowire composites assembled with tobacco mosaic virus as a template has not been reported; at the same time, in this tobacco mosaic virus nanowire On the surface, using the reducibility and reactivity of polydopamine itself, without the need for additional reducing agents and molecular biology methods, it is possible to further conveniently and efficiently load gold nanoparticles and functionalize bioactive molecules.

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