Preparing method for tobacco mosaic virus nanowire composite material with surface functionalized

A technology of tobacco mosaic virus and surface functionalization, which is applied in the fields of nanotechnology, general/multifunctional contrast agent, nanotechnology, etc. for materials and surface science. To solve problems such as application in the field, to achieve the effect of simple process, simplified preparation method, and shortened preparation cycle

Active Publication Date: 2016-02-17
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the coating materials used in the existing methods are all conductive polymers, which are not biocompatible and cannot be applied in the biomedical field.
[0005] In addition, although the method of using tobacco mosaic virus as a template and carrier to load gold nanoparticles and functional modification molecules on its surface has been realized (for example, Chinese patent CN103706800 expresses hexahistidine-modified tobacco by molecular biology methods) The loading of gold nanoparticles on the surface of tobacco mosaic virus by adding a reducing agent to the mosaic virus coat protein; Chinese patent CN102578695 is through electrostatic mutual attraction, and under the action of a reducing agent, it is deposited on the surface of tobacco mosaic virus by chemical deposition Loaded with gold nanoparticles; Chinese patent CN103613647 expresses a cysteine-mutated tobacco mosaic virus coat protein through molecular biol

Method used

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  • Preparing method for tobacco mosaic virus nanowire composite material with surface functionalized
  • Preparing method for tobacco mosaic virus nanowire composite material with surface functionalized
  • Preparing method for tobacco mosaic virus nanowire composite material with surface functionalized

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Disperse 1 mg of tobacco mosaic virus into 2 mL of 100 mM sodium acetate buffer solution with a pH value of 5.5 to prepare a reaction solution with a mass concentration of 0.5 mg / mL. After stirring for 2 hours at 4 ° C, add to the reaction solution Add 1mg of dopamine hydrochloride and 2.4mg of ammonium persulfate, stir and react at 25°C for 4 hours, then add 50uL of 100mM aqueous sodium hydroxide solution several times, and use a wide range of pH test paper in the range of 1-14 Monitor, adjust the pH value of the reaction solution to 8.5 and continue to react for 2 hours, then centrifuge the reaction solution at a speed of 9500rpm for 10min, take the supernatant, and dialyze in pure water for 48 hours with a dialysis bag with a molecular weight cut-off of 1 million Daltons. Hours, the pure water was changed every 8 hours, and finally freeze-dried to obtain the polydopamine-coated tobacco mosaic virus nanowires.

[0042] 1 mg of the above-prepared polydopamine-coated to...

Embodiment 2

[0046] Disperse 1 mg of tobacco mosaic virus into 2 mL of 100 mM sodium acetate buffer solution with a pH value of 5.5 to prepare a reaction solution with a mass concentration of 0.5 mg / mL. After stirring for 2 hours at 4 ° C, add to the reaction solution 1 mg of dopamine hydrochloride and 2.4 mg of ammonium persulfate were added, and the reaction was fully stirred at 25° C. for 24 hours, and samples were taken according to time points to test the absorption value of the ultraviolet-visible spectrum. Then the reaction solution was centrifuged at 9500rpm for 10min, and the supernatant was taken, dialyzed in pure water for 48 hours with a dialysis bag with a molecular weight cut-off of 1 million Daltons, and the pure water was changed every 8 hours, and freeze-dried.

[0047] The ultraviolet-visible absorption spectrum of the tobacco mosaic virus nanowires coated with polydopamine prepared in this example is as follows: Figure 5 As shown in (a), its UV absorption value at 314nm...

Embodiment 3

[0049] Disperse 1 mg of tobacco mosaic virus into 2 mL of 100 mM sodium acetate buffer solution with a pH value of 5.5 to prepare a reaction solution with a mass concentration of 0.5 mg / mL. After stirring for 2 hours at 4 ° C, add to the reaction solution Add 0.2, 0.4, 0.6, 0.8, 1.0, 1.2, 1.4, 1.6, 1.8, 2.0 mg of dopamine hydrochloride and 0.48-4.8 mg of ammonium persulfate with a molar concentration ratio of 1:2, and fully After stirring and reacting for 4 hours, add 50uL of 100mM sodium hydroxide aqueous solution several times, and monitor with a wide range of pH test paper in the range of 1-14 at the same time, adjust the pH value of the reaction solution to 8.5 and continue the reaction for 2 hours. centrifuge for 10 min at a high speed, take the supernatant, dialyze in pure water for 48 hours with a dialysis bag with a molecular weight cut-off of 1 million Daltons, change the pure water every 8 hours, and finally freeze-dry to obtain polymers with different thicknesses on ...

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Abstract

The invention discloses a preparing method for a tobacco mosaic virus nanowire composite material with the surface functionalized. The preparing method includes the following steps that while tobacco mosaic virus head-tail assembly is carried out on the acidic condition, dopamine and an oxidant are subjected to in-situ oxidative polymerization on the outer surface of virus protein to form tobacco mosaic virus nanowires coated with polydopamine, and on the alkaline condition, the own reducibility and reactivity of the surface polydopamine are used for further loading gold nanoparticles and modifying bioactive molecules containing amino groups or sulfydryl groups in a functionalized mode without extra reductant or chemical modification so as to obtain the tobacco mosaic virus nanowire composite material with the surface functionalized. The preparing method has the advantages of being simple in process, convenient, efficient and capable of being widely applied to biological catalysis, biological imaging, tumor targeted probes, nano-drug carriers and other fields of biomedical new nano materials.

Description

technical field [0001] The invention relates to the field of new nanometer materials. More specifically, it relates to a method for preparing a surface-functionalized tobacco mosaic virus nanowire composite material. Background technique [0002] Nanocomposite materials are a new type of nanomaterials, which not only have the advantages of small size effect, high specific surface area, various morphology characteristics and assembly forms of nanomaterials, but also can be combined with other inorganic, organic, polymer and biological functions. The special properties of materials have been widely developed in the fields of industrial high-efficiency catalysis, biomedical engineering and other new functional materials. Therefore, the development of new one-dimensional nanowire composites has great potential application value in biocatalysis, bioimaging, tumor targeting probes and nano drug carriers. [0003] Polydopamine is formed by oxidative polymerization of dopamine in ...

Claims

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Application Information

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IPC IPC(8): A61K47/02A61K47/34A61K47/46A61K31/704A61P35/00A61K49/00B82Y30/00B82Y40/00
CPCA61K31/704A61K47/02A61K47/34A61K47/46A61K49/0002B82Y30/00B82Y40/00
Inventor 周全牛忠伟蒋士冬
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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