Preparation method of a surface-functionalized tobacco mosaic virus nanowire composite material

Abstract

The invention discloses a preparing method for a tobacco mosaic virus nanowire composite material with the surface functionalized. The preparing method includes the following steps that while tobacco mosaic virus head-tail assembly is carried out on the acidic condition, dopamine and an oxidant are subjected to in-situ oxidative polymerization on the outer surface of virus protein to form tobacco mosaic virus nanowires coated with polydopamine, and on the alkaline condition, the own reducibility and reactivity of the surface polydopamine are used for further loading gold nanoparticles and modifying bioactive molecules containing amino groups or sulfydryl groups in a functionalized mode without extra reductant or chemical modification so as to obtain the tobacco mosaic virus nanowire composite material with the surface functionalized. The preparing method has the advantages of being simple in process, convenient, efficient and capable of being widely applied to biological catalysis, biological imaging, tumor targeted probes, nano-drug carriers and other fields of biomedical new nano materials.

Description

Technical field [0001] The invention relates to the field of nano new materials. More specifically, it relates to a method for preparing a surface-functionalized tobacco mosaic virus nanowire composite material. Background technique [0002] Nanocomposites are a new type of nanomaterials, which not only have the advantages of nanomaterials' small size effect, high specific surface area, various morphological features and assembly forms, but also can compound other inorganic, organic, polymer and biological functions. The special properties of materials have been extensively developed in the fields of industrial high-efficiency catalysis, biomedical engineering and other new functional materials. Therefore, the development of new one-dimensional nanowire composite materials has great potential application value in biocatalysis, bioimaging, tumor targeting probes and nano drug carriers. [0003] Polydopamine is formed by oxidative polymerization of dopamine in an alkaline environme...

AI Technical Summary

Problems solved by technology

However, the coating materials used in the existing methods are all conductive polymers, which are not biocompatible and cannot be applied in the biomedical field.

[0005] In addition, although the method of using tobacco mosaic virus as a template and carrier to load gold nanoparticles and functional modified molecules on its surface has been realized (for example, Chinese patent CN 103706800 is expressed through molecular biology methods to obtain hexahistidine modification Tobacco mosaic virus coat protein, and adding a reducing agent to realize the loading of gold nanoparticles on the surface of tobacco mosaic virus; Chinese patent CN 102578695 is through electrostatic mutual attraction, under the action of a reducing agent, chemically deposited on tobacco mosaic leaves The surface of the virus is loaded with gold nanoparticles; Chinese patent CN 103613647 expresses the cysteine-mutated tobacco mosaic virus coat protein through the method of molecular biology, and then modifies the fluorescent molecules and anticancer drugs to the cysteine-mutated Nano-drug carriers are prepared on the surface of the protein), but these methods have complex operation steps, long preparation cycle, need molecular biology methods to modify gene sequence and express protein, and need to add additional reducing agent to reduce to obtain gold nanoparticles and other problems and defects

[0006] So far, the use of biocompatible polydopamine for coating and the preparation of one-dimensional nanowire composites assembled with tobacco mosaic virus as a template has not been reported; at the same time, in this tobacco mosaic virus nanowire On the surface, using the reducibility and reactivity of polydopamine itself, without the need for additional reducing agents and molecular biology methods, it is possible to further conveniently and efficiently load gold nanoparticles and functionalize bioactive molecules.

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