Harpin coding gene (i) hrpZPsgS1(/i) or application of harpinZPsgS1 protein expressed by Harpin coding gene (i) hrpZPsgS1(/i)

Abstract

The invention discloses a Harpin coding gene (i) hrpZPsgS1( / i) and an application of a harpinZPsgS1 protein expressed by the Harpin coding gene (i) hrpZPsgS1( / i). If the obtained harpinZPsgS1 protein is used for treating tobacco plants, the growing of the tobacco plants is promoted; and the resistance of the tobacco plants against tobacco mosaic viruses and tobacco phytophthora diseases is improved obviously, wherein the resistance is not only limited in parts treated by the harpinZPsgS1 protein but also acts on the peripheral leaves and the whole plants, and the expression of PR (pathogenesis related) protein is carried out in the treating process, so that the hrpZPsgS1( / i) can induct the plant systems to obtain disease resistance. The result shows that the hrpZPsgS1 has the application value of being developed into a biogenic pesticide.

Description

technical field

[0001] The invention belongs to the field of biological genetic engineering and relates to the Harpin coding gene wxya PsgS1 or its expressed harpinZ PsgS1 protein application. Background technique

[0002] Soybean bacterial spot is a major disease in my country, especially in northern soybean producing areas, which has brought huge losses to agricultural production. The pathogenic bacteria Pseudomonas syringae pathogenic variety Pseudomonas syringae pv. glycinea ) is also an important plant pathogenic bacteria worldwide. Like other Gram-negative plant pathogenic bacteria, Pseudomonas syringae pv. glycinea have a conservative hrp Gene clusters determine the hypersensitive response (HR) of pathogenic bacteria on non-host plants and disease-resistant plants and the pathogenicity (pathogenicity) on susceptible hosts. hrp The specific gene-encoded product of the gene cluster, harpins, is a class of non-specific protein elicitors, which are rich i...

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