944 results about "Gene cluster" patented technology

A method for associating a gene with a trait exhibited by one or more organisms in a plurality of organisms from a species. A genetic marker map is constructed from a set of genetic markers associated with the plurality of organisms. For each gene in a plurality of genes, a quantitative trait locus analysis is performed using the genetic marker map and a quantitative trait. The quantitative trait locus analysis produces quantitative trait locus data. A quantitative trait comprises an expression statistic for a gene. The expression statistic for a gene is derived from a cellular constituent level that corresponds to the gene in each organism in the plurality of organisms. The quantitative trait locus data are clustered from each quantitative trait locus analysis to form a quantitative trait locus interaction map. Clusters of genes in the map are identified as a candidate pathway group. An expression cluster map is used to refine the candidate pathway group. Multivariate analysis is used to validate the candidate pathway group as a set of genes that are genetically interacting.

Methods are provided for the detection of and determining prognosis of metastatic breast, lung, prostate, and/or pancreatic cancer using various genetic markers, including markers for gene clusters linked by Esx. In one method, breast cancer micrometastases and non-small cell lung cancer metastases or micrometastases are detected in a patient by determining whether the AGR2 or TFF1 genes are overexpressed in a cell sample compared to control lymph node tissue cells. In a further method, the likelihood that a patient diagnosed with breast cancer will respond to hormonal therapy is predicted by determining a higher expression level of the AGR2 gene compared to a control gene. In a further method, a decreased probability of survival for a patient diagnosed with early stage non-small cell lung cancer is predicted by determining a higher expression level of the AGR2 gene compared to a control gene. Kits for practicing the methods of the invention are further provided. Methods are also provided for the identification of markers for which overexpression is indicative of the presence of micrometastatic disease.

The invention relates to methods of evaluating transplant rejection in a host comprising determining a heightened magnitude of gene expression of genes in rejection-associated gene clusters. The disclosed gene clusters include genes that are substantially co-expressed with cytotoxic lymphocyte pro-apoptotic genes, cytoprotective genes and several other cytokine and immune cell genes.

The invention discloses a construction method of a penicillin-producing recombined strain of streptomyces virginiae IBL14. The method involves sequence properties of a beta-lactamase gene containing hydrolysis penicillin and gene clusters producing penicillin, and the whole process of a construction method of the penicillin-producing recombined strain constructed on this basis. Related genes are compiled through the penicillin-producing gene and the CRISPR-Cas I-B gene of streptomyces virginiae IBL14, and therefore the aim of increasing the penicillin yield is achieved. A new route and method are provided for increasing the types of biological medicine, improving the production level and improving the product quality.

Methods for making synthetic gene clusters are described.

The invention discloses a dual-spacer sequence recognition and cleavage CRISPR-Cas9 vector construction, and application thereof in verrucosispora. The invention provides a dual-spacer sequence recognition and cleavage CRISPR-Cas9 vector construction method. A dual-spacer sequence recognition and cleavage CRISPR-Cas9 vector for deleting an ocean verrucosispora abyssomicin gene cluster is successfully constructed by utilizing the method. The experiment proves that the dual-spacer sequence recognition and cleavage CRISPR-Cas9 vector for deleting the ocean verrucosispora abyssomicin gene cluster constructed by the invention can successfully delete the ocean verrucosispora abyssomicin gene cluster.

The invention relates to Streptococcus suis infection in pigs, vaccines directed against those infections and tests for diagnosing Streptococcus suis infections. The invention provides an isolated or recombinant nucleic acid encoding a capsular gene cluster of Streptococcus suis or a gene or gene fragment derivated thereof. The invention further provides a nucleic acid probe or primer allowing species or serotype-specific detection of Streptococcus suis. The invention also provides a Streptococcus suis antigen and vaccine derived thereof.

The invention provides genetically engineered bacteria for efficiently producing N-acetylglucosamine. The genetically engineered bacteria are prepared by the following steps: integrating chromosome deficiency nag DCABE gene clusters of Escherichia coli, respectively connecting 6-glucosamine phosphate synthetase mutant genes and N-acetylglucosamine transferase genes which are respectively mediated by a T7 promoter and a Trc promoter with a gene expression cassette in series, wherein the 6-glucosamine phosphate synthetase mutant genes are obtained by mutating wild 6-glucosamine phosphate synthetase genes from an Escherichia coli W3110 strain source into A38T/R249C/G471S mutants. The genetically engineered bacteria constructed by the invention have the advantages of high N-acetylglucosamine fermentation yield and high strain stability and have wide industrial application prospects.

The present invention provides methods for single nucleotide polymorphism (SNP)-based killer cell immunoglobulin-like receptor (KIR) gene cluster genotyping using the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer. In general, the methods involve amplifying a plurality of target sequences of a plurality of KIR genes, and detecting the presence or absence of a plurality of single SNPs of the plurality of KIR genes by MALDI-TOF mass spectrometry. The invention also features compositions, including arrays of capture primers and optionally extension primers on a substrate surface, and kits, for use in the methods of the invention.

A unique carotenogenic biosynthetic gene cluster has been isolated from Panteoa agglomerans strain DC404, wherein the genetic organization of the cluster is crtE-idi-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

The present invention refers to the gene cluster and genes comprised by the gene cluster which are involved in the biosynthesis of griselimycin and methylgriselimycin and to the use of the gene cluster, genes comprised thereby and proteins encoded thereby for the production of antibiotic agents.

The present invention relates to a transformant, containing a lactate dehydrogenase gene which is introduced into Schizosaccharomyces pombe as a host, in which a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated.

Disclosed are methods and systems for applying independent component analysis (ICA) and other advanced signal processing techniques to automatically identify an optimal number of independent gene clusters and to efficiently separate gene expression data into biologically relevant groups. Embodiments of the methods and systems of the present invention provide an interface that allows the user to review the results at various stages during the analysis, thereby optimizing the type of analysis performed for a specific experiment. Also disclosed are methods and systems to mathematically define the relationship for gene expression within a group of interrelated genes.

The invention relates to a genetically engineered bacterium for high-yield production of hydroxytetrahydropyrimidine as well as a construction method and application of the genetically engineered bacterium. The strain integrates RNA (Ribonucleic Acid) polymerase which is controlled by a xylose promoter and is derived from T7 bacteriophage; a thrA gene for encoding homoserine dehydrogenase I is knocked out; a lysC gene derived from corynebacterium glutamicum, an ectABC gene cluster derived from halomonas elongate and an ectD gene for encoding tetrahydropyrimidine hydroxylase are integrated andare promoted by a strong promoter PT7; a hydroxytetrahydropyrimidine synthesis way is reconstructed; the ectD gene is integrated at a sucCD site of succinyl coenzyme A synthetase and is started by thestrong promoter PT7; the genetically engineered bacterium has the highest level of producing the hydroxytetrahydropyrimidine by a fermentation method which is reported at present.

The invention relates to a method for analyzing the correlation between CT imaging genomics characteristics and gene expression in lung cancer. The method includes: using a semi-automatic segmentationmethod to extract CT imaging genomics characteristics of a segmented tumor were; performing clustering analysis on the basis of preprocessed gene data, and taking a first principal component as a representative of a gene clustering result with a similar expression profile; and finally, using a gene chip saliency analysis algorithm to find the correlation between CT imaging genomics characteristics and gene expression in lung cancer, and verifying and analyzing the result. The invention provides a novel scheme for exploring the relationship between the image characteristics and the gene data,and attempts to find an imaging substitute of the gene, interprets the image characteristics from the gene level, and assists the personalized treatment of the tumor well.

The present invention is generally directed to the evolution of new metabolic pathways and the enhancement of bioprocessing through a process herein termed recursive sequence recombination. Recursive sequence recombination entails performing iterative cycles of recombination and screening or selection to “evolve” individual genes, whole plasmids or viruses, multigene clusters, or even whole genomes. Such techniques do not require the extensive analysis and computation required by conventional methods for metabolic engineering.

A modified S7 polysaccharide is disclosed. The polysaccharide contains 20% less glucose that S7. An S7c6 gene cluster is disclosed. A mutated Sphingomonas is also disclosed.

Two gene clusters have been isolated from an Brevibacterium sp HCU that encode the enzymes expected to convert cyclohexanol to adipic acid. Individual open reading frames (ORF's) on each gene cluster are useful for the production of intermediates in the adipic acid biosynthetic pathway or of related molecules. All the ORF's have been sequenced. Identification of gene function has been made on the basis of sequence comparison and biochemical analysis.

The invention belongs to the technical field of biology and in particular provides a pectate lyase (PL) gene Pcpel1 which is cloned from phytophthora capsici and protein preparation technology thereof. Gene and protein levels prove that the gene is effectively involved in the process that the phytophthora capsici infects hot pepper hosts and results in occurrence of the course of diseases on hot pepper leaves. Plant pathology and cytochemistry technology further prove that after the protein coded by the gene is inoculated onto the hot pepper leaves, obvious withering and shrinking occur on theinoculated parts of the leaves and the cell walls on the affected parts of the leaves are obviously degraded, namely the gene code is an important protein related to the course of diseases or is possibly an important target pathogenic gene of a phytophthora capsici PL gene cluster. The invention provides important technical reserve for further developing phytophthora capsici molecule detection technology.

The present invention provides cloning sequencing, analyzing, function research of a biosynthesis gene cluster of an antibiotic-Azinomycin B having antitumor activity produced by streptomyces, and its application. The whole gene cluster includes 34 genes: one repeatedly using I type polyketide synthase gene; two naphthalene ring modification enzyme genes; 8 non-ribosomal polypeptide skeleton synthesis and modification enzyme genes; 11 non-natural amino acid structure unit synthase genes; 1 resistance gene; 3 post modification enzyme genes and 8 genes which functions are not determined. The genetic operation of the biosynthesis gene breaks the synthesis of Azinomycin B; the precursor compound is produced by the heterologous expression of synthesis gene and modification gene of naphthalene ring. The gene of the invention and the protein can be used for searching and finding compound or gene, protein applied in medical, industry or agriculture.

The invention discloses a human beta globin gene containing a human beta globin gene promoter, a recombinant adeno-associated virus vector containing the human beta globin gene, and a method for preparing the recombinant vector. The recombinant adeno-associated virus vector inserts an HS2 segment, an HS3 segment and an HS4 segment of a human beta globin gene cluster enhancer core sequence and a human beta globin gene sequence containing the human beta globin gene promoter into repeated sequence ITRs on the reverse terminal of an adeno-associated virus. The recombinant vector has high transfection efficiency; and mediated exogenous genes can be expressed in vivo for a long time, have good safety and can be used for gene therapy of beta Mediterranean anemia.

The invention discloses a biosynthetic gene cluster of romatic-polyketide atypical fluostatins and applications thereof. The nucleotide sequence of the biosynthetic gene cluster of fluostatins derived from micromonospora rosaria SCSIO N160 (preservation No: CCTCC NO: M2012392) is shown in the 1st-40128th base sequences in SEQ ID NO. 1, and contains 32 genes. Genes and proteins thereof provided by the invention can be used for seeking and finding compounds or genes and proteins for medicine, industry or agriculture.