44 results about "Gene cluster" patented technology

This invention relates to a method for producing chondroitin with improved safety and extracellular secretion levels, belonging to the field of genetic engineering. Using the probiotic *Escherichia coli* Nissle 1917 as the host, this invention designs methods to increase chondroitin production by studying the chondroitin synthase KfoAC and the chondroitin-linking Kdo synthase. The relationship between chondroitin synthesis and secretion in *E. coli* was clarified, and the specificity of the chondroitin-linking Kdo was investigated, resulting in an extracellular chondroitin production of 0.32 g / L. Subsequently, the introduction of the protein KfoB from the chondroitin synthesis gene cluster increased the extracellular chondroitin production to 0.51 g / L. Further screening and optimization of the expression of transport proteins resulted in an extracellular chondroitin production of 0.73 g / L in shake flasks. Further optimization using a 7-L fermenter resulted in an extracellular chondroitin production of 5.9 g / L.

This invention discloses a *Bacillus laterosporus* strain for the stable production of nonribosomal cyclic lipolipeptide and its construction method. The M1A gene in the Bogorol biosynthetic gene cluster is knocked out using CRISPR-Cas9 technology to block the Bogorol pathway, which competes with Brevicidine for substrates. Subsequently, the pMCCas9-S48 plasmid, which integrates S8 and S41 sgRNAs and their homologous arms as repair templates, is used to simultaneously knock out S8 and S41 family peptidase genes, preventing the expression of extracellular degradation enzymes. This invention also provides the application of the above strain in the preparation of nonribosomal cyclic lipolipeptide of Brevicidine and a method for preparing the nonribosomal cyclic lipolipeptide. This strain, through reducing metabolic competition and preventing product degradation, exhibits rapid cell growth, stable production of Brevicidine, and significantly reduced degradation rate, resulting in a 66.13% increase in fermentation yield compared to the unmodified strain. This invention solves the problems of degradation and unstable yield in the production of Brevicidine, provides an effective technical approach for the industrial production of Brevicidine, and provides important technical support for the development and application of novel antimicrobial peptide drugs.

The application discloses a construction method of a multiplex PCR reaction system for detecting and identifying Podosphaera species and application thereof. The method comprises the following steps: obtaining whole genome sequences of multiple Podosphaera species, and performing multiple sequence alignment to obtain specific genes and specific gene clusters of each species; taking the genes in the specific gene clusters of each species as templates, designing multiple upstream primers and downstream primers according to a conventional primer design method to generate primer pairs, and excluding unreasonable primer pairs and primer pairs with low sensitivity and specificity; according to the size of the amplification products, the primers are freely combined and matched to generate four pairs of mixed multiplex primers, and the multiplex PCR reaction conditions are optimized to obtain a multiplex PCR system capable of specifically detecting Podosphaera and simultaneously identifying single and multiple species. The multiplex PCR method for detecting and identifying Podosphaera provided by the application can quickly and accurately complete the detection of Podosphaera and identify the species identity of Podosphaera.

The present application belongs to the field of biotechnology, and particularly relates to a biosynthesis gene cluster of fungal polyketide compounds and application thereof. The present application provides a heterologous expression method of an aspertaichunol compound biosynthesis gene combination, and a compound aspertaichunol B is obtained through the method, and a compound aspertaichunol A is prepared through acid hydrolysis of the aspertaichunol B, which has important significance for future research and development of an efficient biosynthesis method for preparing the aspertaichunol compound.

The present application belongs to the field of synthetic biology and functional food technology, and provides a swallowing safety-oriented xanthan gum directional biosynthesis method. In view of the problem that existing xanthan gum is difficult to reduce the risk of swallowing residue and aspiration by relying on viscosity regulation alone, a multi-dimensional evaluation system combining interfacial tribology and extensional rheology is established to determine the side chain modification parameters suitable for swallowing disorder food. By chromosome scarless reconstruction of xanthan gum synthesis gene cluster, the gumF 、 gumG gene is knocked out and the gumL promoter is replaced, an engineering strain without antibiotic resistance gene residue and with genetic stability is constructed, and directional synthesis of xanthan gum with specific side chain structure is realized. The obtained structure customized xanthan gum has a lower mucosal interfacial friction coefficient and a more optimal extensional rheological property, and can synergistically realize interfacial lubrication and bolus cohesion, significantly reducing the wall residue in the pharynx, and keeping the consistency stable in complex food matrix. The present application can provide a safe, stable and industrialized functional xanthan gum raw material for swallowing disorder food.

This invention discloses a method for preparing self-healing concrete based on microbial gene programming, belonging to the field of building materials technology. The method includes: constructing a recombinant engineered strain incorporating a urease gene cluster and a carbonic anhydrase gene; preparing a spore suspension and protecting it with a three-layer microencapsulation system of sodium alginate / chitosan / gelatin; preparing nutrient-releasing microspheres encapsulating calcium lactate and urea in a polylactic acid-glycolic acid copolymer; and mixing cement, standard sand, water, fly ash, microencapsulated spore particles, nutrient-releasing microspheres, nano-silica, and modified bentonite to prepare self-healing concrete. This invention enhances the urease activity of the strain through genetic engineering, protects the spores using a three-layer microencapsulation technology, and combines a nutrient-releasing system and nano-synergists to achieve efficient self-repair of concrete cracks.

The present application belongs to the field of genetic engineering and microbial fermentation technology, and particularly relates to a recombinant Escherichia coli for producing N-acetylglucosamine as well as a construction method and application thereof. The present application takes Escherichia coli as a chassis, knocks out the nagDCABE gene cluster, the manXYZ gene cluster and the serA gene of the genome of the Escherichia coli, and introduces the glmS, GNA1 and serA genes into the Escherichia coli through a vector for expression. The yield of the Escherichia coli for fermenting GlcNAc provided by the present application can reach more than 100 g / L.

This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using a method comprising the following steps: using *Corynebacterium glutamicum* as the starting strain, feedback inhibition by aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed. Based on this, the activity of any one or at least two of the following—diaminobutyrate acetyltransferase EctA, pyruvate carboxylase Pyc, or aspartate kinase LysC—is enhanced, and / or the activity of any one or at least two of the following—maloquinone oxidoreductase Mqo or homoserine dehydrogenase Hom—is reduced to further increase ectoine yield.

This invention relates to an engineered bacterium that heterologously expresses Indochrome C based on the cold-stressed NRPS gene cluster. The engineered bacterium is... Escherichia coli The pET41a-Indochrome C-Cluster, with accession number GDMCC No. 66715, is disclosed in this invention. The invention also discloses a method for constructing this engineered bacterium. By reconstructing the Indochrome C NRPS gene cluster using synthetic biology techniques, this invention solves the problems of long fermentation cycles and insufficient yields in the original strain, significantly improving pigment production efficiency and industrial application potential.

This invention relates to the field of microbial technology and discloses a biocontrol strain for controlling peanut stem rot, wherein the biocontrol strain is *Bacillus pilaris* (…). Paenibacillus peoriae This invention also provides a biocontrol agent containing this strain and its preparation method. Genomic analysis revealed that this strain contains gene clusters encoding active substances such as fusarium and polymyxin, exhibiting significant antagonistic activity against peanut stem rot fungus, and broad-spectrum inhibitory activity against various plant pathogenic fungi, including wheat stem base rot fungus and rapeseed sclerotinia. Furthermore, this strain possesses the ability to secrete auxin, produce proteases, and produce cellulases. The prepared inoculum, applied through root irrigation, can construct a preventative biological barrier in the rhizosphere, providing both disease prevention and growth promotion effects. This invention provides a highly efficient germplasm resource for the green control of crop diseases.

The application belongs to the field of agricultural biotechnology and microbial engineering technology, and particularly relates to a biosynthesis gene cluster of cystobactamids and application. The nucleotide sequence of the biosynthesis gene cluster is shown as SEQ ID NO:1. The application discloses a novel biological function of cystobactamids except for antibiotics, broadens the application scene, and has a good development prospect in the field of agricultural biological control.

Provided herein are stoichiometric Gas Vesicle Expression Systems (GVES), sets of polynucleotide constructs, and related vectors, cells, compositions, and methods configured for robust expression of Gas Vesicle Gene Clusters (GVGCs) in mammalian cells, particularly primary and immune cells. The GVES comprises distinct gene modules for the primary structural protein (gvpA / B) and assembly factors (AF1, AF2), operably configured to achieve a stoichiometric expression ratio, quantified by Dosage Index (DI), wherein the gvpA / B module is expressed at least 2-fold higher than the AF1 and AF2 modules.

The application relates to the field of environmental microorganism technology and discloses a salt-tolerant aerobic denitrification bacterium and a method for simultaneously realizing denitrification and carbonate precipitation and carbon fixation. Zobellella denitrificans A36, which is preserved in the Guangdong Microbial Culture Collection Center and has a preservation number of GDMCC No: 66789 and a preservation date of August 7, 2025. The Zobellella denitrificans A36 has complete denitrification key functional genes and carbonic anhydrase related gene clusters; can induce the formation of carbonate precipitation in a salt-containing heterotrophic denitrification system, fixes CO2 generated by the system into inorganic carbonate, and effectively reduces CO2 emission. Under preferred conditions, the removal rate of nitrate nitrogen can reach 93.8%, and stable carbonate carbon fixation effect is realized.

The present application relates to the field of genetic engineering and bioengineering, and particularly relates to a genetically engineered surfactin-producing bacterium and application thereof.The genome of the genetically engineered bacterium comprises the following: an expression frame of T7 RNA polymerase; an expression frame of a surfactin synthesis gene cluster srfAABCD; and an expression frame of a long-chain fatty acid-coenzyme A ligase gene lcfA; the expression frame of the surfactin synthesis gene cluster srfAABCD and the expression frame of the long-chain fatty acid-coenzyme A ligase gene lcfA further comprise a T7 promoter.The present application realizes efficient synthesis of surfactin by Bacillus subtilis, and the final shake flask yield reaches more than 19.3 g / L, which has great industrial application potential.

The application belongs to the technical field of life science and computer science, and provides a gene cluster and protein complex detection method and system based on multi-supergroup coupling, which comprises the following steps: obtaining gene and protein data of a species from a public biological database, constructing a gene supergroup and a protein supergroup based on the relationship between the gene data and the protein data of the species; constructing an inter-layer edge relationship between the gene supergroup and the protein supergroup; constructing a multi-supergroup coupling random block model with shared potential functional modules; obtaining a community membership matrix of proteins and genes in different relationship supergroups through joint inference, and then identifying the gene cluster and the protein complex. Through the method, the accuracy of gene cluster and protein complex detection is improved.

This invention discloses a strain of *Bacillus belyceae* with broad-spectrum antibacterial activity and its application in the prevention and treatment of necrotic enteritis in poultry. The *Bacillus belyceae* strain provided by this invention (… Bacillus from Velez The genome of strain GDG25CB056 is approximately 3.91 Mb in size, contains no virulence factors, and is rich in gene clusters encoding non-ribosomal peptides and polyketide synthases. Studies have confirmed that this strain exhibits significant growth advantages, strong tolerance to high temperatures, gastric acid, and bile salts, which facilitates intestinal colonization. It can produce siderophores and various enzymes, and has overcome the technical bottleneck of a narrow antibacterial spectrum, possessing broad-spectrum antibacterial activity, especially strong specific antagonistic activity against Clostridium perfringens. In vivo experiments have shown that this strain can significantly reduce the Clostridium perfringens load in the cecum of chicks with necrotizing enterocolitis, alleviate intestinal congestion and swelling, repair damaged intestinal villi, and enhance mucosal barrier function, providing a safe and efficient strain resource for the prevention and treatment of bacterial enteric diseases.

This invention belongs to the fields of microbiology and molecular biology, specifically relating to a homologous recombinase combination in myxobacteria and its application in gene editing. Specifically, this invention provides four pairs of homologous recombinase systems capable of efficiently mediating in vivo recombination in myxobacteria. Each pair includes a protein YqaJ with exonuclease function and a protein RecT with single-strand annealing function. This invention provides a method for myxobacterial gene editing: under the action of homologous recombinase pairs, mutant strains are obtained through positive screening using resistance tags. The resistance tags are then successfully removed through the combined action of the Cre / loxP system and the reverse screening gene galK. This method achieves a gene editing efficiency of up to 100%, has a short cycle time, requires no purification, and can be used for iterative deletion of multiple genes to achieve genome simplification in myxobacteria, or for inserting functional promoters and other elements to activate silent gene clusters. This method is of great significance for the bioexploration and functional genomics research of myxobacteria.

This invention belongs to the field of plant molecular breeding, and specifically discloses a gene related to rice fertility restoration. OsRf19 This invention addresses the cumbersome process of identifying restorer lines for the existing Fujian abortive cytoplasmic male sterility (CMS-FA / OsRf19) system by developing a method for creating tightly linked artificial haplotypes, the development of InDel molecular markers, and their application in rice fertility. Through bioinformatics analysis, this invention identifies a fertility restoration gene associated with the rice sporophytic sterility system CMS-FA. OsRf19 A specific region closely linked to the PPR gene cluster and with extremely low similarity to the loci was edited using CRISPR / Cas12a gene editing technology, resulting in multiple large deletions. Based on these genotype sequence variations, a set of InDel molecular markers was developed to realize the editing of this artificially created restoration gene. OsRf19 Rapid identification of closely linked artificial haplotypes accelerates the breeding process of molecular marker-assisted selection in the rice CMS-FA system.

This invention relates to the field of gene technology, specifically disclosing a method for enhancing the secretion of secondary metabolites based on the regulation of quorum sensing signal molecules. This method activates the expression of bacterial secondary metabolite synthesis gene clusters through bacterial-fungal interaction, comprising the following steps: (a) co-culturing bacteria and fungi in contact or non-contact mode; (b) during co-culturing, the fungi secrete the quorum sensing signal molecule farnesol; (c) the bacteria sense farnesol through a two-component system, activating the expression of secondary metabolite synthesis genes. This invention effectively overcomes the gene silencing problem caused by metabolic load or lack of regulation in natural strains by activating the originally trace expression of secondary metabolite synthesis gene clusters in bacteria using the fungal-derived quorum sensing signal molecule farnesol.

This invention belongs to the field of gene technology and discloses a bacterial strain B11 antagonistic to soft rot disease of Amorphophallus muelleri. Isolated from the rhizosphere soil of Amorphophallus muelleri, it was identified as the golden subspecies of *Pseudomonas aeruginosa*. This strain effectively inhibits the growth of the soft rot pathogen Pcc, reducing the incidence and disease index of soft rot in Amorphophallus muelleri. It also possesses the ability to dissolve organophosphates and produce siderophores, and exhibits broad-spectrum antagonistic activity against various fungal diseases of Amorphophallus muelleri, including white mold, leaf spot, and dry rot. Whole-genome analysis shows that the B11 genome is 6.77 Mb in size, with a GC content of 62.81%, encoding 5,964 genes, including gene clusters related to antibacterial activity such as phenazine, nitropyrrolizin, and siderophores. This strain has good development and application potential in the control of soft rot in Amorphophallus muelleri.

This invention discloses a method for heterologous production of 7-aminodeacetoxycephalosporanic acid using *Aspergillus nidulans*. This invention targets a *Aspergillus nidulans* strain that heterologously produces 7-aminodeacetoxycephalosporanic acid. Genes derived from the biosynthetic gene clusters pcbAB / C, isopentine N-acetyltransferase gene, and cefE gene, or their mutants, are assembled into a *Aspergillus nidulans* expression vector. Co-expression is performed in a *Aspergillus nidulans* basal cell strain to obtain a recombinant strain, successfully achieving heterologous expression of 7-aminodeacetoxycephalosporanic acid in filamentous fungi. Experiments show that the yield can reach 0.13 g / L at the shake-flask level. The strain constructed in this invention provides a good heterologous expression system for studying the synthesis and regulatory mechanism of 7-aminodeacetoxycephalosporanic acid.

This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using the following steps: using Corynebacterium glutamicum as the starting strain, feedback inhibition of aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed; based on this, the activity of any one or at least two combinations of aspartate semialdehyde dehydrogenase Asd, aspartate transaminase AspB, or 6-phosphogluconate dehydrogenase Gnd is enhanced to further increase the yield of ectoine.

This invention discloses an engineered bacterium producing tetracrine, its construction, and its application. The invention utilizes a strong promoter to drive the high expression of regulatory genes ovmZn and ovmWn in *Streptomyces neyagawa*, constructing a recombinant engineered strain Sne / pZWn; alternatively, it utilizes a strong promoter to drive the high expression of homologous genes of both, including the regulatory genes ovmZ and ovmW from *Streptomyces chromogenicus*, in *Streptomyces neyagawa*, constructing a recombinant engineered strain Sne / pZW. These engineered strains can express previously silenced gene clusters, activating tetracrine biosynthesis and laying the foundation for the development and application of tetracrine.

This invention discloses a high-glutathione-producing engineered Escherichia coli strain and its applications, belonging to the field of biotechnology; the engineered Escherichia coli strain is named ZMGSH01F, with accession number CCTCC M 20252236; the engineered Escherichia coli strain is based on Escherichia coli BL21(DE3) as the starting strain, with gene clusters knocked out. ptsH‐ ptsI‐crr And integrate at this site glf‐glk Fusion genes, knockout gene clusters gsiA‐gsiB‐gsiC Integrate the endogenous glutathione transporter gene at this site. cycDC Knockout of γ-glutamyltransferase gene ggt Simultaneously expressing the recombinant plasmid pRSFDuet- gshAB K177E‑E275K‑V513A - ppk2 The engineered Escherichia coli strain can efficiently produce glutathione through fermentation culture using glucose, monosodium glutamate, cysteine, and glycine as substrates.

The application discloses a kind of recombinant bacillus subtilis and its method for efficiently producing tetrahydropyrimidine using bean dregs hydrolysate, belong to microbial fermentation technical field.First, with bacillus subtilis 168 as host, heterologous import from Halomonas elongate ectABC Gene cluster constructs basic expression strain.Then through CRISPR-Cas9 technology, key genes of competitive metabolic branch hom And dapA Are precisely knocked out, successfully block the conversion of precursor material to threonine, methionine and lysine, significantly improve the carbon flux to the direction of tetrahydropyrimidine synthesis.Not only solve the equipment corrosion problem caused by traditional salt-tolerant bacteria fermentation, but also overcome the conventional engineering bacteria when using bean dregs fermentation byproduct many, low conversion rate bottleneck.Finally, the recombinant strain fermentation 36 h, tetrahydropyrimidine production can reach 28.68 g / L, provide extremely creative solution for the high-value utilization of agricultural waste bean dregs and the low-cost green production of tetrahydropyrimidine.

The application belongs to the field of molecular biology, and particularly relates to a method for efficiently constructing large fragment deletion of zebrafish helt gene and application. The method for efficiently constructing large fragment deletion of zebrafish helt gene is applied to constructing an animal model for treating and drug screening of Alzheimer disease caused by large fragment deletion of genome. The application also provides a method for efficiently constructing large fragment deletion of zebrafish helt gene. Through design of a target and a primer, a mutant with large fragment deletion of zebrafish helt gene is obtained. The CRISPR / Cas system is used to perform genome large fragment deletion in a zebrafish helt gene cluster. The length of the deleted genome fragment ranges from 1.4 kb, and the average efficiency reaches more than 60%, which greatly improves the efficiency of genome large fragment deletion.

The application comprises a RAS gene cluster-based pediatric AML prognosis model construction method, which relates to the cross field of bioinformatics and medical technology. It comprises: S1: data acquisition and integration, standardization and integration processing of expression data; S2: sample typing, obtaining at least two subtypes with different prognoses; S3: prognosis model construction; S4: clinical correlation verification, constructing a visual prognosis evaluation system integrating risk stratification and clinical characteristics; S5: immune infiltration correlation verification, establishing the correlation between risk stratification and immune infiltration characteristics; S6: drug sensitivity correlation verification, determining the correlation between risk stratification and drug sensitivity; S7: key gene verification. The application realizes the precise risk stratification of pediatric AML patients by integrating RAS signal pathway gene characteristics and clinical data, and the prognosis model is verified by training set and verification set, which provides a standardized scientific research model framework and reliable data support, and provides an efficient auxiliary tool for pediatric AML related scientific research.

The application discloses a kossakia sp. LY-1 and application thereof in promoting rice growth, which is preserved in China Center for Type Culture Collection and has a preservation number CCTCC NO: M 20242320. The strain carries complete nifHDKY-ENX-BQ-VZW nitrogen fixation gene cluster, and has a nitrogenase content of 160 ng / mL. Meanwhile, the strain has four growth promoting functions of dissolving inorganic phosphorus (57 mg / L), producing indole acetic acid (19.76 mg / L) and producing siderophore (48%). Under the condition of nitrogen-free water culture, the strain can significantly promote the growth of rice seedlings, and the stem length is increased by 25.7% and the biomass is increased by 45.6% compared with a control, so that the strain provides a functional strain resource for green production of rice.

This invention belongs to the field of synthetic biology and discloses tetracycline compounds modified with a polysaccharide group at the C8 position, their biosynthetic methods, and applications. The tetracycline compounds with a polysaccharide group modification at the C8 position provided by this invention have a novel structure; the trisaccharide and hexasaccharide groups present at the C8 position are not found in known tetracycline natural products or tetracycline skeleton antibiotics. The biosynthetic method involves using a polysaccharide group derived from *Streptomyces rubrum*. ech Gene clusters, in which mis This invention expresses the gene cluster in the *Streptomyces micranthum* host, fully utilizing the different biosynthetic enzymes contained in two similar but different tetracycline natural product biosynthetic gene clusters to synthesize novel compounds that neither host bacterium can synthesize. These compounds exhibit significant inhibitory activity against *Staphylococcus aureus*, *Enterococcus faecalis*, and *Enterococcus faecium*. This invention provides new candidate compounds for the innovative development of tetracycline-based antibacterial drugs and is of great significance to the development of microbial drug resources.

The invention discloses a gamma-PGA synthetase gene cluster pgsBCA '. The gamma-PGA synthetase gene cluster pgsBCA' is formed by sequentially connecting genes pgsB, pgsC and pgsA 'in series, the genes pgsB and pgsC are derived from a gamma-PGA synthetase gene cluster BA-pgsBCA of the bacillus altitudinis; the gene pgsA'is derived from a gamma-PGA synthetase gene cluster BM-pgsB 'C' A 'of bacillus megatherium. Bacillus amyloliquefaciens CF of which a genome knockout polyglutamate synthetase gene cluster is used as a host, and a recombinant polyglutamate synthetase gene cluster pgsBCA'is subjected to free expression through plasmids, so that a gene engineering strain capable of synthesizing gamma-L-PGA is obtained. When the strain is used for producing gamma-L-PGA in a 5L fermentation tank through amplification fermentation, the maximum yield can reach 10.5 g / L. Besides, the gamma-poly-L-glutamic acid is free of cytotoxicity and low in immunogenicity, so that the gamma-poly-L-glutamic acid has a wide application prospect in the fields of preparation of wound dressings, in-vivo absorption materials and the like. The invention provides a new material basis and technical support for application and development of polyglutamic acid in the fields of medicine and related biomedicine.