208 results about "Gene cluster" patented technology

The invention discloses enterobacter mori capable of resisting fish aeromonas and application of the enterobacter mori, the bacterial strain is classified and named as enterobacter mori SCH0241, the enterobacter mori SCH0241 is preserved in the China Center for Type Culture Collection on October 17, 2024, and the preservation number is CCTCC NO: M 20242236. The enterobacter mori SCH0241 disclosed by the invention is derived from the intestinal tract of high-stress-resistance healthy hybrid crucian carassius auratus No. 2, has good contact dependence antibacterial activity on aeromonas veronii, and can improve the capability of resisting aeromonas veronii infection of zebra fish. Through construction of an insertion mutation library and phenotype screening, it is found that a gene cluster of SCH0241, which exerts contact inhibition toxicity, is T6SS-2. The enterobacter mori SCH0241 disclosed by the invention provides a new powerful tool for biological prevention and treatment of fish diseases caused by aeromonas veronii.

The invention relates to the technical field of microorganisms, and discloses a pseudomonas putida strain and a preparation method and application thereof.The strain is pseudomonas putida which is separated from soil and has the capacity of dissimilatory reduction of nitrate into ammonium and is classified into a new pseudomonas putida strain based on GTDB-TK. A dissimilatory NOreductive gene cluster, namely an nirBD gene cluster, exists on a genome of the gene, nirK / nirS genes are deleted, and NOs can be reduced into NH under the anaerobic culture condition. According to the application of the pseudomonas putida strain in improvement of soil nitrogen management, the pseudomonas putida strain can be mixed with organic fertilizer for use so as to enhance the effective utilization and conversion capacity of nitrogen in soil. By means of the pseudomonas putida strain, NON can be reduced into NHH under the anaerobic condition, the culture method is simple, the growth speed is high, variation is not prone to occurring, and a brand new microbial resource is provided for soil nitrogen cycle research.

Methods for making synthetic gene clusters are described.

The disclosure provides methods for increasing saturated, monounsaturated, and poly unsaturated fatty acid production using the PUFA gene cluster plasmid and the gene regulator pfaR.

The invention relates to the field of PQQ production, in particular to an engineering bacterium for producing pyrroloquinoline quinone and a preparation method of the engineering bacterium. According to the invention, different broad-host shuttle vectors are used, PQQ is expressed in denitrifying hyphomicrobe to synthesize related gene clusters, denitrifying hyphomicrobe engineering bacteria with high yield of PQQ are successfully constructed, and the engineering bacteria have the characteristics of rapid growth, high fermentation yield and the like, and lay a foundation for realizing green production of PQQ.

The present disclosure relates to systems, non-transitory computer-readable media, and methods that analyze gene perturbation machine learning embeddings and clinical observation data sets utilizing machine learning, explainability models, and causal discovery models to generate causal predictions between one or more genes and clinical outcomes. Indeed, in one or more implementations, the disclosed systems identify gene perturbation embeddings generated from cells exposed to perturbations. For instance, the disclosed systems select a cluster of genes from a plurality of genes by applying a clustering model to the gene perturbation embeddings. In some instances, the disclosed systems select gene targets from the cluster of genes by using a machine learning classification model trained on a plurality of features of the clinical observation data set. Moreover, in some instances, the disclosed systems generate the causal prediction from the gene targets and the clinical observation data set utilizing a causal discovery model.

This invention relates to a method for producing chondroitin with improved safety and extracellular secretion levels, belonging to the field of genetic engineering. Using the probiotic *Escherichia coli* Nissle 1917 as the host, this invention designs methods to increase chondroitin production by studying the chondroitin synthase KfoAC and the chondroitin-linking Kdo synthase. The relationship between chondroitin synthesis and secretion in *E. coli* was clarified, and the specificity of the chondroitin-linking Kdo was investigated, resulting in an extracellular chondroitin production of 0.32 g / L. Subsequently, the introduction of the protein KfoB from the chondroitin synthesis gene cluster increased the extracellular chondroitin production to 0.51 g / L. Further screening and optimization of the expression of transport proteins resulted in an extracellular chondroitin production of 0.73 g / L in shake flasks. Further optimization using a 7-L fermenter resulted in an extracellular chondroitin production of 5.9 g / L.

The invention discloses a method for increasing the fermentation yield of mernomycin A based on a reinforced chassis strain glycosyl donor. Specifically, in the heterologous expression process of a sirnomycin A biosynthetic gene cluster in a chassis strain Streptomyces albus J1074, a glycosyl donor synthesis module gene and a resistance gene (MaPBP) are introduced at the same time, and on the basis of improving a glycosyl biosynthetic donor, the physiological tolerance of the chassis strain is also enhanced. Compared with a high-yield strain Streptomyces albus LX03 (CGMCC NO. 20985) of which the tolerance is only optimized, the yield of the strain Streptomyces albus LX02: pJQK610 subjected to genetic engineering optimization is improved by 285%, and the yield reaches 114 mg / L. The strain disclosed by the invention has the advantages that the yield of the strain Streptomyces albus LX02: pJQK610 is improved by 285%, and the yield of the strain Streptomyces albus LX02: pJQK610 is improved by 285%. The invention provides a high-yield technology for improving heterologous expression of the mernomycin A through collaborative optimization of precursor supply and physiological tolerance, and provides reference for efficient production of the medicine.

The invention discloses a gas-producing vibrio strain for producing prodigiosin and a prodigiosin synthetic gene cluster, and belongs to the technical field of microorganisms. The invention relates to a strain B1D10 for producing prodigiosin, the classification name of the strain B1D10 is Vibrio gazogenes, the strain B1D10 is preserved in Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC NO: 66510, the preservation date is June 13, 2025, and the preservation address is No.59 building, No.100 Courtyard, Xianlie Middle Road, Guangzhou. The strain B1D10 contains a prodigiosin synthetic gene cluster consisting of 16 genes. The strain is rapid in propagation and can be applied to large-scale fermentation production of prodigiosin.

The application discloses a construction method and application of a genetically engineered corynebacterium glutamicum for synthesizing a bacteriochlorophyllide amino acid shinorine, and belongs to the field of genetic engineering and fermentation technology. The genetically engineered corynebacterium glutamicum is constructed by taking corynebacterium glutamicum ATCC 13032 as a host strain, knocking out a competitive pathway gene, integrating a key enzyme gene in multiple copies on a genome, knocking out an acid production pathway gene and a serine dehydrogenase gene, and expressing a gene cluster for synthesizing shinorine on a plasmid, so that the synthesis yield of shinorine in a microbial cell is improved. The strain can produce shinorine in a flask, and 1.12 g / L of shinorine can be accumulated in 72 hours. In fed-batch fermentation in a 50 L fermenter, the strain can be cultured to 8.6 g / L.

This invention discloses a *Bacillus laterosporus* strain for the stable production of nonribosomal cyclic lipolipeptide and its construction method. The M1A gene in the Bogorol biosynthetic gene cluster is knocked out using CRISPR-Cas9 technology to block the Bogorol pathway, which competes with Brevicidine for substrates. Subsequently, the pMCCas9-S48 plasmid, which integrates S8 and S41 sgRNAs and their homologous arms as repair templates, is used to simultaneously knock out S8 and S41 family peptidase genes, preventing the expression of extracellular degradation enzymes. This invention also provides the application of the above strain in the preparation of nonribosomal cyclic lipolipeptide of Brevicidine and a method for preparing the nonribosomal cyclic lipolipeptide. This strain, through reducing metabolic competition and preventing product degradation, exhibits rapid cell growth, stable production of Brevicidine, and significantly reduced degradation rate, resulting in a 66.13% increase in fermentation yield compared to the unmodified strain. This invention solves the problems of degradation and unstable yield in the production of Brevicidine, provides an effective technical approach for the industrial production of Brevicidine, and provides important technical support for the development and application of novel antimicrobial peptide drugs.

The present application relates to a group of peptide compounds with antiviral activity, the structure of the peptide compound is shown in formula (1): the virus is a respiratory virus, especially influenza virus or coronavirus. The present application also relates to a gene cluster for biosynthesis of the peptide compound by microorganisms, the gene cluster is Streptomyces sp. CPCC 200451 genome chromosome 1:7,822,964-7,875,615, with a full length of 52.6 kb.

The invention provides a method for rapidly detecting microbial species, and particularly, the method comprises the following steps: constructing a double-level database and a corresponding gene cluster; constructing a reference matrix X based on the database and the gene cluster; comparing to-be-detected third-generation sequencing data with the gene cluster to generate equivalence classes, and constructing a counting matrix Y based on the equivalence classes and the gene cluster; based on the counting matrix Y and the reference matrix X, determining the gene abundance in the to-be-detected sample; and determining microbial species in the sample to be detected based on the gene abundance. The rapid pathogen detection method provided by the invention is high in analysis speed, fully utilizes the advantages of the three-generation sequencing data, and can more directly obtain the microorganism composition information of the sample; the method is low in computing resource demand, easy to deploy and high in precision detection capability.

The application discloses a construction method of a multiplex PCR reaction system for detecting and identifying Podosphaera species and application thereof. The method comprises the following steps: obtaining whole genome sequences of multiple Podosphaera species, and performing multiple sequence alignment to obtain specific genes and specific gene clusters of each species; taking the genes in the specific gene clusters of each species as templates, designing multiple upstream primers and downstream primers according to a conventional primer design method to generate primer pairs, and excluding unreasonable primer pairs and primer pairs with low sensitivity and specificity; according to the size of the amplification products, the primers are freely combined and matched to generate four pairs of mixed multiplex primers, and the multiplex PCR reaction conditions are optimized to obtain a multiplex PCR system capable of specifically detecting Podosphaera and simultaneously identifying single and multiple species. The multiplex PCR method for detecting and identifying Podosphaera provided by the application can quickly and accurately complete the detection of Podosphaera and identify the species identity of Podosphaera.

The present application belongs to the field of biotechnology, and particularly relates to a biosynthesis gene cluster of fungal polyketide compounds and application thereof. The present application provides a heterologous expression method of an aspertaichunol compound biosynthesis gene combination, and a compound aspertaichunol B is obtained through the method, and a compound aspertaichunol A is prepared through acid hydrolysis of the aspertaichunol B, which has important significance for future research and development of an efficient biosynthesis method for preparing the aspertaichunol compound.

The present application belongs to the field of synthetic biology and functional food technology, and provides a swallowing safety-oriented xanthan gum directional biosynthesis method. In view of the problem that existing xanthan gum is difficult to reduce the risk of swallowing residue and aspiration by relying on viscosity regulation alone, a multi-dimensional evaluation system combining interfacial tribology and extensional rheology is established to determine the side chain modification parameters suitable for swallowing disorder food. By chromosome scarless reconstruction of xanthan gum synthesis gene cluster, the gumF 、 gumG gene is knocked out and the gumL promoter is replaced, an engineering strain without antibiotic resistance gene residue and with genetic stability is constructed, and directional synthesis of xanthan gum with specific side chain structure is realized. The obtained structure customized xanthan gum has a lower mucosal interfacial friction coefficient and a more optimal extensional rheological property, and can synergistically realize interfacial lubrication and bolus cohesion, significantly reducing the wall residue in the pharynx, and keeping the consistency stable in complex food matrix. The present application can provide a safe, stable and industrialized functional xanthan gum raw material for swallowing disorder food.

Aiming at a lactic acid return loss phenomenon existing in a process for producing lactic acid by microbial fermentation, the invention firstly provides application of a target gene in inhibiting lactic acid return loss in lactic acid production by a microbial fermentation method, and the target gene is a lactic acid permease gene lutP and / or an iron-sulfur oxidase gene cluster lutABC. On the basis of the application, the invention also provides a method for inhibiting lactic acid return consumption in lactic acid production by a microbial fermentation method, and a bacillus coagulans engineering strain with low lactic acid return consumption and high sugar-acid conversion rate. According to the method, effective inhibition of intracellular transport and / or metabolism of lactic acid is realized, so that the problem of lactic acid return consumption in a process for producing lactic acid through microbial fermentation is solved, and the technical effect is remarkable. According to the engineering strain, a LutR palindromic sequence in a lactic acid permease gene lutP promoter region is modified, and / or an iron-sulfur oxidase gene cluster lutABC is inactivated, so that transmembrane transport and / or intracellular metabolism of lactic acid are / is effectively inhibited, a lactic acid return loss phenomenon is remarkably weakened, the sugar-acid conversion efficiency is improved, the fermentation process flow is simplified, the production cost is reduced, and the engineering strain is suitable for industrial production. And the method has important value for large-scale production.

This invention discloses a method for preparing self-healing concrete based on microbial gene programming, belonging to the field of building materials technology. The method includes: constructing a recombinant engineered strain incorporating a urease gene cluster and a carbonic anhydrase gene; preparing a spore suspension and protecting it with a three-layer microencapsulation system of sodium alginate / chitosan / gelatin; preparing nutrient-releasing microspheres encapsulating calcium lactate and urea in a polylactic acid-glycolic acid copolymer; and mixing cement, standard sand, water, fly ash, microencapsulated spore particles, nutrient-releasing microspheres, nano-silica, and modified bentonite to prepare self-healing concrete. This invention enhances the urease activity of the strain through genetic engineering, protects the spores using a three-layer microencapsulation technology, and combines a nutrient-releasing system and nano-synergists to achieve efficient self-repair of concrete cracks.

The invention discloses a 13-hydroxyglucopiericidin A heterologous expression engineering strain, a construction method of the 13-hydroxyglucopiericidin A heterologous expression engineering strain and an application of the 13-hydroxyglucopiericidin A heterologous expression engineering strain. According to the engineering strain, Streptomyces avicularis SUKA17 is taken as a host, and heterologous synthesis of the chalicimycin glycoside, especially 13OH-GPA, is realized by introducing a biological synthesis gene cluster of the chalicimycin glycoside; the bacterial strain is classified and named as Streptomyces sp. SK-SA23E, and the preservation number of the bacterial strain is GDMCC NO.67271. The invention further discloses a preparation method of the bacterial strain. According to the invention, the efficient heterologous expression of the biological synthesis gene cluster of the chalcone glycoside is realized for the first time, the yield of the 13OH-GPA in the engineering strain is increased by 2.8 times compared with that of the original production strain, the relative content of the 13OH-GPA in the chalcone glycoside is increased to 48.1% from 23.6%, and the method is suitable for large-scale preparation of the 13OH-GPA.

This invention provides a method and a production strain for increasing the yield of carbamoyl tobramycin, belonging to the field of genetic engineering technology. By knocking out the tobR gene, which encodes an Lrp / AsnC family transcriptional regulator, within the tobramycin synthesis gene cluster region of the *Streptoalloteichus tenebrarius* genome, and by overexpressing the tobO gene, which encodes a TauD / TfdA family oxidase directly acted upon by TobR, using a constitutive strong promoter, a hybrid mutant strain was obtained, exhibiting a significantly increased fermentation yield of carbamoyl tobramycin. The shake-flask fermentation level of carbamoyl tobramycin by this hybrid mutant strain reached 3.76 g / L, a 42.5% increase compared to the 2.64 g / L of the starting strain. The advantages of this invention are: by inactivating the tobR gene and simultaneously overexpressing the tobO gene in *S. tenebrarius*, a high-yielding carbamoyl tobramycin strain was constructed. This strain can increase the fermentation yield of carbamoyl tobramycin without increasing the cost of additional culture medium, thus reducing production costs and showing significant industrial application potential.

The present application belongs to the field of genetic engineering and microbial fermentation technology, and particularly relates to a recombinant Escherichia coli for producing N-acetylglucosamine as well as a construction method and application thereof. The present application takes Escherichia coli as a chassis, knocks out the nagDCABE gene cluster, the manXYZ gene cluster and the serA gene of the genome of the Escherichia coli, and introduces the glmS, GNA1 and serA genes into the Escherichia coli through a vector for expression. The yield of the Escherichia coli for fermenting GlcNAc provided by the present application can reach more than 100 g / L.

The invention relates to a methanophilic genetically engineered bacterium for producing gamma-polyglutamic acid (gamma-PGA) as well as a construction method and application of the methanophilic genetically engineered bacterium, EQU2420670 gene is knocked out on a host bacterium genome, and meanwhile, an exogenous gamma-PGA synthetase encoding gene cluster pgsBCAE under the control of an inducible arabinose promoter (Pbad) is introduced at the site. The construction method comprises the following steps: obtaining a multi-cistron structure DNA fragment pgsBCAE for coding gamma-PGA synthetase through artificial gene synthesis in combination with a codon optimization strategy, and constructing the expression module Pbad-pgsBCAE under the control of a Pbad promoter. A suicide vector pK18mobsacB carrying a SacB sucrose reverse selection system is used for constructing a gene replacement vector, upstream and downstream homologous arms of an EQU2420670 gene on a genome of methanophilic bacteria M. buryatase 5GB1 are designed on two sides, an EQU2420670 site is replaced by a Pbad-pgsBCAE expression module, the methanophilic genetically engineered bacterium PGA is obtained, and the methanophilic genetically engineered bacterium PGA is applied to production of gamma-PGA and has important economic and social significance; the gamma-PGA produced by the engineering methanophilic bacteria by using methane as a carbon source and an energy source can be used as a cosmetic additive; the invention provides a novel low-carbon biosynthesis approach for commercial production and application of gamma-PGA, and has a good industrial application prospect.

This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using a method comprising the following steps: using *Corynebacterium glutamicum* as the starting strain, feedback inhibition by aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed. Based on this, the activity of any one or at least two of the following—diaminobutyrate acetyltransferase EctA, pyruvate carboxylase Pyc, or aspartate kinase LysC—is enhanced, and / or the activity of any one or at least two of the following—maloquinone oxidoreductase Mqo or homoserine dehydrogenase Hom—is reduced to further increase ectoine yield.

Provided herein are stoichiometric Gas Vesicle Expression Systems (GVES), sets of polynucleotide constructs, and related vectors, cells, compositions, and methods configured for robust expression of Gas Vesicle Gene Clusters (GVGCs) in mammalian cells, particularly primary and immune cells. The GVES comprises distinct gene modules for the primary structural protein (gvpA / B) and assembly factors (AF1, AF2), operably configured to achieve a stoichiometric expression ratio, quantified by Dosage Index (DI), wherein the gvpA / B module is expressed at least 2-fold higher than the AF1 and AF2 modules.

The invention relates to the technical field of biology, and particularly discloses a construction method and application of escherichia coli engineering bacteria for overexpressing uric acid degradation gene clusters. According to the invention, a mixed promoter box is inserted into Escherichia coli, overexpression coding is carried out on genes of regulatory protein YgeV and key genes in a uric acid degradation pathway, and the Escherichia coli engineering bacteria which can overcome inhibition of intestinal glucose on uric acid degradation and enable the Escherichia coli engineering bacteria to overexpress uric acid degradation gene clusters are successfully constructed. The coliform engineering provided by the invention provides a new strategy for prevention and treatment of hyperuricemia and gout.

The invention belongs to the field of molecular biology and genetic engineering, and discloses a chemiluminescent reporter plasmid pBBR402 and application thereof in detection of the transcription level of a target gene of acetobacter pasteurianus. The plasmid comprises a replication element (oriV and rep) from a pBBR1MCS-2 plasmid, a kanamycin resistance gene from a pMS402 plasmid, a promoter insertion site (BamHI / AvaI / XhoI), a luxCDABE reporter gene cluster and a T1 transcription terminator, and the kanamycin resistance gene, the promoter insertion site (BamHI / AvaI / XhoI), the luxCDABE reporter gene cluster and the T1 transcription terminator are obtained from the pBBR1MCS-2 plasmid. The plasmid can be inserted into a to-be-detected gene promoter of acetobacter pasteurianus to drive luxCDABE expression. During application, the recombinant plasmid is transferred into acetobacter pasteurianus, the OD value and the LUM value of the living bacteria liquid are directly detected, and the transcription level is quantified by the LUM / OD value. The method is easy to operate, high in sensitivity and suitable for gene regulation and control research of acetobacter pasteurianus.

The invention relates to the technical field of precise nutrition, and particularly discloses an alpha-linolenic acid personalized supplement judgment method and system based on fatty acid metabolism related genotypes. The method comprises the following steps: acquiring genotype information of at least one single nucleotide polymorphism site of an individual in a fatty acid desaturase gene cluster and / or a fatty acid elongase gene cluster; evaluating the expected metabolic response efficacy of the individual to alpha-linolenic acid supplementation; and judging whether alpha-linolenic acid is suitable for supplementing n-3 polyunsaturated fatty acid or not according to an evaluation result. The technical problem that an existing one-cut type alpha-linolenic acid supplement scheme is ineffective to people with low hereditary metabolism is solved, and precise nutrition intervention based on the genetic background is achieved.

The invention discloses a 5 / 8 / 5 chitosan clostridium diterpenoid compound, a biosynthetic gene cluster and application of the biosynthetic gene cluster, and relates to the technical field of biological medicines. A 5 / 8 / 5 chitin type diterpenoid biosynthetic gene cluster Thm in trichoderma harzianum is identified through a genome mining system, chitin diterpenoid compounds with diversified structures are obtained by means of heterologous expression, the obtained compounds are subjected to anti-thrombotic activity comprehensive evaluation, and the result shows that the compounds 6 and 31 can obviously inhibit vascular thrombosis, the compound 6 has a remarkable inhibition effect on collagen-induced platelet aggregation, and the compound 31 has a better inhibition capability on thrombin-induced platelet activation. The compounds 6 and 31 show powerful antithrombotic effects in artery, vein and pulmonary artery thrombosis models, so that powerful technical support is provided for development of antithrombotic drugs.

The invention discloses a method for reducing the molecular weight of gamma-PGA based on a polycistron expression vector, which comprises the following steps: cloning a gamma-polyglutamic acid synthetase gene cluster pgsBCA of bacillus licheniformis into corynebacterium glutamicum F343 with high yield of glutamic acid in a polycistron expression mode for exogenous expression to obtain a recombinant strain pBCA; compared with a recombinant strain pBpCpA expressed by a monocistron, the recombinant strain pBCA carrying a polycistron expression system has the advantage that the molecular weight of gamma-PGA generated by the recombinant strain pBCA is reduced by 15.98%. Therefore, the polycistron expression system is an effective method for reducing the molecular weight of the gamma-PGA.

This invention relates to an engineered bacterium that heterologously expresses Indochrome C based on the cold-stressed NRPS gene cluster. The engineered bacterium is... Escherichia coli The pET41a-Indochrome C-Cluster, with accession number GDMCC No. 66715, is disclosed in this invention. The invention also discloses a method for constructing this engineered bacterium. By reconstructing the Indochrome C NRPS gene cluster using synthetic biology techniques, this invention solves the problems of long fermentation cycles and insufficient yields in the original strain, significantly improving pigment production efficiency and industrial application potential.