The invention belongs to the technical field of biomass energy preparation, and particularly relates to a method for producing acetone-ethanol-butanol (ABE) by fermentation with a straw hydrolysate asa raw material. The method specifically includes the steps of straw pretreatment, biological enzymolysis, preparation of an ABE fermentation culture solution, a fermentation hydrolysate and the like.According to the method, when butanol is fermented with a straw acid hydrolysate as a raw material and in combination with the fermentation treatment of specific microbial strains, the expression level of related key enzymes in a solvent metabolism pathway can be significantly affected by adding glutathione, and thus chemical compositions of cell membranes of the strains are changed, and the butanol production capacity is improved. According to the method, straws are pretreated into hydrolysate and then directly fermented to produce ABE, the complex detoxification process is avoided, so thatthe production cost is reduced, and the industrialization of ABE fermentation is promoted; and furthermore, through the optimization of the fermentation process, especially the addition and utilization of the glutathione in the fermentation process, the fermentation yield of the ABE is further improved, and the ABE industrial production is promoted.