Nitrogen defect control method for production of ethanol through fermentation of synthesis gas with mixed bacteria

A technology of mixing bacteria and syngas, used in fermentation, biofuels, etc., to reduce emissions

Inactive Publication Date: 2017-02-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A composite flora proposed by the University of Science and Technology of China and its application in syngas fermentation and alcohol production (CN105087441A) also does not involve nitrogen deficiency regulation methods

Method used

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  • Nitrogen defect control method for production of ethanol through fermentation of synthesis gas with mixed bacteria
  • Nitrogen defect control method for production of ethanol through fermentation of synthesis gas with mixed bacteria
  • Nitrogen defect control method for production of ethanol through fermentation of synthesis gas with mixed bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Add 50g of homoacetogenic bacteria enrichment to 500mL liquid medium containing 1.0g / L yeast extract, filled with CO / CO 2 / H 2 (4:3:3) to a standard atmospheric pressure, adjust the pH=7.0, and carry out the first stage of cultivation at 37°C. After 6 days of cultivation, the homoacetogenic bacteria grow to a stable state and enter the second stage. Take 10g of the enrichment after the first stage of cultivation and inoculate it into 100mL of basal medium containing 0.05g / L yeast extract, and place it on a constant temperature shaker at 37°C at 100rpm. The components and concentrations of the basic elements in the medium are the same as Same as first stage: K 2 HPO 4 0.25g / L, KH 2 PO 4 0.25g / L, MgCl 2 ·6H 2 O0.3g / L, BES (bromoethanesulfonate) 10.5g / L. Charge CO / CO 2 / H 2 (4:3:3) to a standard atmospheric pressure to maintain pH 7.0. Gas consumption and organic product formation were measured by gas chromatography. The results showed that the ethanol accumul...

Embodiment 2

[0028] Add 50g of homoacetogenic bacteria enrichment to 500mL containing 0.5g / L NH 4 In the liquid medium of Cl, filled with CO / CO 2 / H 2(4:3:3) to a standard atmospheric pressure, adjust the pH=7.0, and carry out the first stage of cultivation at 37°C. After 6 days of cultivation, the homoacetogenic bacteria grow to a stable state and enter the second stage. Take 10g of the enrichment after the first stage culture to inoculate 100mL without NH 4 In the basal culture medium of Cl (other basic components and concentrations are the same as in Example 1 except the nitrogen source), culture on a constant temperature shaker at 37° C. and 100 rpm. Charge CO / CO 2 / H 2 (4:3:3) to a standard atmospheric pressure to maintain pH 7.0. Gas consumption and organic product formation were measured by gas chromatography. The results showed that the maximum ethanol accumulation in the second stage was 7.8mmol / L, which was 40.2% higher than the maximum ethanol accumulation in the first sta...

Embodiment 3

[0031] Add 50g of homoacetogenic bacteria enrichment to 500mL liquid medium containing 10g / L yeast extract, filled with CO / CO 2 / H 2 (4:3:3) to a standard atmospheric pressure, adjust the pH=7.0, and carry out the first stage of cultivation at 37°C. After 6 days of cultivation, the homoacetogenic bacteria grow to a stable state and enter the second stage. Take 10 g of the enrichment after the first-stage culture and inoculate it into 100 mL of basal medium containing 0.1 g / L yeast extract (basic element components and concentrations are the same as in Example 1), and place it on a constant temperature shaker at 37°C and 100 rpm for cultivation . Charge CO / CO 2 / H 2 (4:3:3) to a standard atmospheric pressure to maintain pH 7.0. Gas consumption and organic product formation were measured by gas chromatography. The results showed that the ethanol accumulation in the second stage was 10.9mmol / L, which was 45.9% higher than the ethanol concentration in the first stage ( imag...

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Abstract

The invention belongs to the field of biological energy sources and particularly relates to a nitrogen defect control method for production of ethanol through fermentation of synthesis gas with mixed bacteria. The method comprises steps as follows: a first stage: mixed bacteria are cultured to grow in a fermentation medium to reach a stable state, that is, the yield of fermentation products is stable and unchanged; a second stage: the mixed bacteria growing to reach the stable state are cultured at the second stage, the nitrogen concentration of the fermentation medium at the stage is controlled to be lower than that at the first stage, the mixed bacteria are the same type of acetogenic bacteria which come from cow dung, urban excess sludge and enriched products thereof, as ethanol is a non-growth coupling type product of the acetogenic bacteria, insufficient nitrogen nutrients possibly cause abnormal cell metabolism, cells enter a non-growth state or dormant state, the condition not beneficial to growth of the acetogenic bacteria is created through nitrogen nutrient defects, so that the metabolism pathway is changed from the acid producing phase to the solvent producing phase, and ethanol production is controlled.

Description

technical field [0001] The invention belongs to the field of biological energy, and in particular relates to a nitrogen deficiency control method for producing ethanol by fermenting synthetic gas with mixed bacteria. Background technique [0002] Syngas fermentation to produce ethanol is a new technology to produce ethanol from biomass, that is, complete gasification of biomass to obtain CO, H 2 , CO 2 Syngas as the main component, and then use microorganisms to convert the syngas into ethanol. This technology does not require expensive enzymes and reagents such as acids and alkalis, which reduces production costs, and in theory microorganisms can completely utilize gases, which is an environmentally friendly technology. [0003] Mixed culture biotechnology is a very promising technology in recent years. Its concept is to combine traditional elements of environmental biotechnology from the perspective of bioengineering, to maximize products like industrial biotechnology wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/06
CPCC12P7/06Y02E50/10
Inventor 刘和符波田淼徐晓宇刘宏波
Owner JIANGNAN UNIV
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