Method for detecting content of linoleic acid in tissues by liquid chromatography-tandem mass spectrometry and diagnostic kit of colorectal cancer

Abstract

The invention provides a method for detecting the content of linoleic acid in tissues by liquid chromatography-tandem mass spectrometry and a diagnostic kit of colorectal cancer. The method comprises the following steps: grinding a tissue sample into powder in liquid nitrogen; homogenizing the tissue powder with iced methanol containing BHT (Butylated Hydroxytoluene) and centrifuging; adding an interior label into supernatant and carrying out derivatization treatment with n-butanol; dispersing and dissolving the mixture by a mobile phase again to obtain a sample to be loaded and detected; analyzing the sample to be loaded and detected with the liquid chromatography-tandem mass spectrometry to obtain the content of the linoleic acid in the tissue sample. Furthermore, a metabolic enzyme of a linoleic acid pathway is subjected to expression analysis and a result shows that gene expression of FADS1 (Fatty Acid Desaturase 1), FADS2 (Fatty Acid Desaturase 2), ELOVL2 (elongase of very long chain fatty acid 2) and ELOVL5 (elongase of very long chain fatty acid 5) in the linoleic acid pathway is increased. The method provided by the invention can be used for detecting changes of the content of the linoleic acid of tumor tissues and normal tissues of patients with the colorectal cancer and expression changes of genes in a related metabolism pathway, and important reference is provided for diagnosis and treatment of the colorectal cancer and post-period nutrition support.

Description

technical field [0001] The invention belongs to the field of tumor medicine, and relates to liquid chromatography-tandem mass spectrometry (LC-MS / MS method) detection of linoleic acid content in intestinal tissue samples and a diagnostic kit for colorectal cancer. Background technique [0002] Colorectal cancer (Colorectal cancer) is a common malignant tumor in the digestive system, and its death rate ranks fourth in the world. Although endoscopy is helpful for the diagnosis of colorectal cancer, its application is limited due to economic conditions and patient compliance. At present, carcinoembryonic antigen (CEA) is commonly used in the early screening of ovarian cancer and digestive tract tumors, but its detection sensitivity and specificity need to be improved. The diagnosis of colorectal cancer is of great significance to improve the survival rate of patients. [0003] Because essential fatty acids cannot be synthesized in the body, they must be provided by food, main...

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Problems solved by technology

However, LC-MS / MS derivatization methods are too tedious and time-consuming

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