Recombinant escherichia coli for producing L-valine and application thereof

A valine and amino acid technology, applied in the direction of recombinant DNA technology, microorganism-based methods, bacteria, etc., can solve the problems of unbalanced reducing power, etc., to reduce production costs, increase yield and conversion rate, and cell tolerance, The production process is stable and easy to operate
CN113278568AActive Publication Date: 2021-08-20ANHUI HUAHENG BIOTECH +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ANHUI HUAHENG BIOTECH
Publication Date
2021-08-20

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Abstract

According to the invention, the amino acid dehydrogenase activity of an L-valine fermentation strain is enhanced, and / or an Entner-Doudoroff (ED) metabolism pathway is activated, such that the problem of unbalanced reducing power during the L-valine fermentation process is solved, therefore the yield and the conversion rate of the L-valine produced by the escherichia coli are improved, and the one-step method anaerobic fermentation of the L-valine is achieved.
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Description

Technical field

[0001] The present invention relates to the construction method of producing recombinant microorganisms of L-proline, and recombinant microorganisms obtained by the constructing method are specifically recombinant E. coli, and a method of producing L-proline by fermentation.

[0002] Inventory background

[0003] L-proline is one of the three branched-chainaminoacid, BCAA, belongs to the essential amino acid, and cannot be synthesized in people and animals, and can only be obtained from the outside world. At present, L-proline is widely used in the field of food and medicine, mainly including food additives, nutritional supplements and flavoring agents; widely used in cosmetic preparation, and used as antibiotics or herbicide precursors; Increased feed mass and ratio demand, the role of L-proline in the feed additive industry will become more important in the feed additive industry, the demand will become bigger and bigger, and the future market has great potential...

Claims

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