Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of mevalonic acid metabolism pathway inhibitor and alphavirus in preparing antitumor drug

A technology of mevalonate and inhibitor, applied in the field of biomedicine, can solve problems such as unclear mechanism of action

Active Publication Date: 2019-07-09
GUANGZHOU VIROTECH PHARMA
View PDF7 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the patent 201510990705.7 previously applied by the inventor, chrysophanol and its derivatives are used as anti-tumor synergists of oncolytic viruses. The combination of the two can reduce the survival rate of tumor cells to 39.6%, but there is a big difference in its anti-cancer strength. room for improvement, in addition, the mechanism of action of this combined application is not yet clear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of mevalonic acid metabolism pathway inhibitor and alphavirus in preparing antitumor drug
  • Application of mevalonic acid metabolism pathway inhibitor and alphavirus in preparing antitumor drug
  • Application of mevalonic acid metabolism pathway inhibitor and alphavirus in preparing antitumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0128] Example 1 siRNA targeting 3-hydroxy-3-methylglutaryl-CoA reductase HMGCR and M1 virus significantly increased the morphological lesions of human intestinal cancer and pancreatic cancer cell lines.

[0129] Material:

[0130] Human intestinal cell carcinoma HCT-116 (purchased from the Cell Bank of the Chinese Academy of Sciences), human pancreatic cell carcinoma Capan-1 (purchased from ATCC), SW1990 (purchased from ATCC), M1 virus (preservation number CCTCC V201423), high-glucose DMEM medium (purchased from Corning), inverted phase contrast microscope.

[0131] method:

[0132] Cells were seeded in a 35mm culture dish, and the following interference treatment was performed when the cell confluence reached 60%; first, Lipofectamine RNAiMAX solution was prepared with optimized medium (Opti-MEM), diluted according to 2 μL per culture dish: 198 μL, and mixed; Secondly, prepare the siRNA solution with the optimized medium (Opti-MEM), dilute it according to 1.8μL:198μL per c...

Embodiment 2

[0137] Example 2 Combined treatment of siRNA targeting 3-hydroxy-3-methylglutaryl-CoA reductase HMGCR with M1 virus significantly reduced the survival rate of human intestinal cell carcinoma lines.

[0138] Material:

[0139] Human intestinal cell carcinoma HCT-116 (purchased from the Cell Bank of the Chinese Academy of Sciences), M1 virus (preservation number CCTCCV201423), high-glucose DMEM medium (purchased from Corning).

[0140] method:

[0141] a) Cell culture: human intestinal cell carcinoma HCT-116 was grown in DMEM complete medium containing 10% FBS, 100U / ml penicillin and 0.1mg / ml streptomycin; all cell lines were placed in 5% CO 2 , cultured and subcultured in a closed incubator with a constant temperature of 37°C (95% relative humidity), and observed the growth with an inverted microscope. The cells were subcultured every 2-3 days, and the cells in the logarithmic growth phase were taken for formal experiments.

[0142] b) Cells were seeded in a 24-well plate, 3...

Embodiment 3

[0147] Example 3 Statins and M1 virus significantly increased the morphological lesions of human intestinal cell carcinoma lines and promoted the replication of M1 virus. Material:

[0148] Human intestinal cell carcinoma HCT-116 (purchased from the Cell Bank of the Chinese Academy of Sciences), M1 virus (preservation number CCTCCV201423), high-glucose DMEM medium (purchased from Corning), and an inverted phase-contrast microscope.

[0149] method:

[0150] a) Cell culture: human intestinal cell carcinoma HCT-116 was grown in DMEM complete medium containing 10% FBS, 100U / ml penicillin and 0.1mg / ml streptomycin; all cell lines were placed in 5% CO 2 , cultured and subcultured in a closed incubator with a constant temperature of 37°C (95% relative humidity), and observed the growth with an inverted microscope. The cells were subcultured every 2-3 days, and the cells in the logarithmic growth phase were taken for formal experiments.

[0151] b) Cell treatment and morphological...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of biomedicine and relates to application of a mevalonic acid metabolism pathway inhibitor and alphavirus in preparing antitumor drug. The invention finds for the first time that the mevalonic acid metabolism pathway inhibitor can be used for preparing an alphavirus antitumor synergist. The invention further relates to a drug composition and a drug set which respectively contain the mevalonic acid metabolism pathway inhibitor and the alphavirus and application of the inhibitor and the alphavirus in treating tumor, especially for tumor insensitive to the alphavirus.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of the combination of mevalonate metabolic pathway inhibitors and alphaviruses in the preparation of antitumor drugs. Background technique [0002] Oncolytic virus is a kind of replication-competent virus that selectively infects and kills tumor cells without damaging normal cells. Oncolytic virus therapy (oncolytic virotherapy) is an innovative tumor-targeted treatment strategy, which uses natural or genetically engineered viruses to selectively infect tumor cells and replicate in tumor cells to achieve targeted dissolution, The effect of killing tumor cells, but no damage to normal cells. [0003] M1 virus (Alphavirus M1) belongs to the genus Alphavirus (Alphavirus). The M1 virus can selectively cause the death of tumor cells without affecting the survival of normal cells, and it has a very good application prospect in anti-tumor. However, different tumors have differ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K45/06A61P35/00
CPCA61K45/06A61K35/768A61K2300/00A61P35/00A61K31/404A61K31/40A61K31/4709A61K31/216A61K31/713C12N7/00C12N2770/36132
Inventor 颜光美梁剑开朱文博张海鹏林园蔡静龚守芳
Owner GUANGZHOU VIROTECH PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com